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List of works by Carlos M.G.A. Fontes

A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum

scientific article published on 01 April 2016

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli.

scientific article published in January 2017

A family 6 carbohydrate-binding module potentiates the efficiency of a recombinant xylanase used to supplement cereal-based diets for poultry

scientific article published on 01 October 2004

A novel α-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum

scientific article (publication date: 2013)

A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets

scientific article published on 03 November 2019

Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins

scientific article published on 23 May 2017

Biodistribution of a (67)Ga-labeled anti-TNF VHH single-domain antibody containing a bacterial albumin-binding domain (Zag).

scientific article published on 17 January 2014

Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes.

scientific article

Carbohydrate Depolymerization by Intricate Cellulosomal Systems

scientific article published in January 2017

Carcass fat partitioning and meat quality of Alentejana and Barrosã young bulls fed high or low maize silage diets

scientific article published on 08 November 2012

Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors.

scientific article published on 8 April 2015

Cellulosome assembly: paradigms are meant to be broken!

scientific article published on 26 March 2018

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates

scientific article

Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice

scientific article published on 01 November 1997

Combined Crystal Structure of a Type I Cohesin: MUTATION AND AFFINITY BINDING STUDIES REVEAL STRUCTURAL DETERMINANTS OF COHESIN-DOCKERIN SPECIFICITIES.

scientific article published on May 2015

Common Inhibition of Both β-Glucosidases and β-Mannosidases by Isofagomine Lactam Reflects Different Conformational Itineraries for Pyranoside Hydrolysis

scientific article published on 01 November 2004

Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions.

scientific article published on 10 February 2017

Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition.

scientific article published on 13 June 2016

Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.

scientific article published on 28 October 2016

Construction of GH16 β-glucanase mini-cellulosomes to improve the nutritive value of barley-based diets for broilers.

scientific article

Crop beta-glucanase activity limits the effectiveness of a recombinant cellulase used to supplement a barley-based feed for free-range broilers

scientific article published on 01 May 2008

Crystal structure of a cellulosomal family 3 carbohydrate esterase from Clostridium thermocellum provides insights into the mechanism of substrate recognition

scientific article

Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation.

scientific article published on 13 June 2006

Crystallization and preliminary X-ray crystallographic analysis of a novel α-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.

scientific article

Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.

scientific article published on January 2015

Deciphering ligand specificity of a Clostridium thermocellum family 35 carbohydrate binding module (CtCBM35) for gluco- and galacto- substituted mannans and its calcium induced stability.

scientific article

Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins.

scientific article

Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors

scientific article

Direct supplementation of diet is the most efficient way of enriching broiler meat with n-3 long-chain polyunsaturated fatty acids

scientific article published in January 2013

Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants

scientific article published on 24 January 2019

Discovery of hyperstable carbohydrate-active enzymes through metagenomics of extreme environments

scientific article published on 21 October 2019

Diverse specificity of cellulosome attachment to the bacterial cell surface

scientific article published on 07 December 2016

Doppel gene polymorphisms in Portuguese sheep breeds: insights on ram fertility.

scientific article published on 11 October 2008

Effect of reduced dietary protein and supplementation with a docosahexaenoic acid product on broiler performance and meat quality

scientific article published on 01 January 2014

Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal.

scientific article published on 17 August 2006

Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability

article published in 2009

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin-dockerin interactions.

scientific article

Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum.

scientific article

Evidence for temporal regulation of the two Pseudomonas cellulosa xylanases belonging to glycoside hydrolase family 11.

scientific article published on August 2002

Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis.

scientific article published on 23 April 2018

Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets.

scientific article

Expression, purification, crystallization and preliminary X-ray analysis of CttA, a putative cellulose-binding protein from Ruminococcus flavefaciens

scientific article published on 22 May 2015

Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities

scientific article

Family 6 carbohydrate-binding modules display multiple beta1,3-linked glucan-specific binding interfaces

scientific article published on 19 August 2009

Fatty acid composition, conjugated linoleic acid isomers and cholesterol in beef from crossbred bullocks intensively produced and from Alentejana purebred bullocks reared according to Carnalentejana-PDO specifications.

scientific article published on 3 October 2005

Fatty acid composition, including isomeric profile of conjugated linoleic acid, and cholesterol in Mertolenga-PDO beef

article

Functional and structural characterization of family 6 carbohydrate-binding module (CtCBM6A) of Clostridium thermocellum α-L-Arabinofuranosidase.

scientific article

Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum.

scientific article published on 10 December 2009

Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.

scientific article

Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.

scientific article

Genetic background and diet impact beef fatty acid composition and stearoyl-CoA desaturase mRNA expression.

scientific article published on 7 March 2013

High-Throughput Production of Oxidized Animal Toxins in Escherichia coli

scientific article published on 01 January 2019

High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay

scientific article published on 01 January 2019

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

scientific article (publication date: 17 January 2017)

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction.

scientific article published on 3 May 2018

Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes.

scientific article published on August 1999

Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus

scientific article published on May 1, 1998

Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface

scientific article published on 14 August 2019

Improving the lipid nutritive value of poultry meat through the incorporation of a dehydrated leguminous-based forage in the diet for broiler chicks.

scientific article published in August 2008

Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase.

scientific article published on 4 May 2012

Influence of pasture intake on the fatty acid composition, and cholesterol, tocopherols, and tocotrienols content in meat from free-range broilers.

scientific article

Influence of slaughter season and muscle type on fatty acid composition, conjugated linoleic acid isomeric distribution and nutritional quality of intramuscular fat in Arouquesa-PDO veal.

scientific article published on 12 March 2007

Inhibition of ovine in vitro fertilization by anti-Prt antibody: hypothetical model for Prt/ZP interaction

scientific article published on 26 March 2013

Insights into the Structural Determinants of Cohesin—Dockerin Specificity Revealed by the Crystal Structure of the Type II Cohesin from Clostridium thermocellum SdbA

scientific article published on 01 June 2005

Insights into the molecular determinants of substrate specificity in glycoside hydrolase family 5 revealed by the crystal structure and kinetics of Cellvibrio mixtus mannosidase 5A.

scientific article published on 10 March 2004

Irradiation effect on fatty acid composition and conjugated linoleic acid isomers in frozen lamb meat.

scientific article published on 5 June 2007

Is prnt a pseudogene? Identification of ram Prt in testis and ejaculated spermatozoa.

scientific article

Molecular Cloning, Expression and Biochemical Characterization of a Family 5 Glycoside Hydrolase First Endo-Mannanase (RfGH5_7) from Ruminococcus flavefaciens FD-1 v3

scientific article published on 01 November 2019

Molecular determinants of ligand specificity in family 11 carbohydrate binding modules: an NMR, X-ray crystallography and computational chemistry approach.

scientific article

Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16.

scientific article published on 29 November 2016

Molecular organization and protein stability of the Clostridium thermocellum glucuronoxylan endo-β-1,4-xylanase of family 30 glycoside hydrolase in solution

scientific article published on 05 April 2019

NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein

scientific article published in November 2013

Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode

scientific article

Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall

scientific article published on 29 March 2019

Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase

scientific article published on 02 June 2018

Novel modular enzymes encoded by a cellulase gene cluster in Cellvibrio mixtus

scientific article published on 27 September 2006

Overexpression, crystallization and preliminary X-ray characterization of Ruminococcus flavefaciens scaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein.

scientific article published on 23 July 2014

Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum.

scientific article published on 30 November 2013

Overexpression, purification, crystallization and preliminary X-ray characterization of the fourth scaffoldin A cohesin from Acetivibrio cellulolyticus in complex with a dockerin from a family 5 glycoside hydrolase

scientific article published on 23 July 2014

Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens.

scientific article published on 25 March 2011

Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-β-1,4-glucanase B (CelB) from Bacillus halodurans

scientific article published on 10 May 2014

Pasture intake improves the performance and meat sensory attributes of free-range broilers.

scientific article published in January 2008

Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus

scientific article published on October 1, 1997

Prion-like Doppel gene polymorphisms and scrapie susceptibility in Portuguese sheep breeds

scientific article published on 26 November 2009

Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants

scientific article

Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A.

scientific article published on 28 July 2015

Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.

scientific article published on 30 June 2011

Purification, crystallization and preliminary X-ray characterization of the third ScaB cohesin in complex with an ScaA X-dockerin from Acetivibrio cellulolyticus

scientific article

Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose

scientific article

Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module.

scientific article published on 18 July 2015

Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality.

scientific article published in October 2008

Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens.

scientific article published on July 2008

Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

scientific article published on 6 February 2015

Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome

scientific article

Single versus dual-binding conformations in cellulosomal cohesin-dockerin complexes

scientific article

Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure

scientific article (publication date: 15 March 2013)

Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11)

scientific article

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C.

scientific article

Structural and biochemical properties of lichenase from Clostridium thermocellum.

scientific article published on March 2009

Structure and Function of an Arabinoxylan-specific Xylanase

scientific article published on March 4, 2011

Structure and activity of two metal ion-dependent acetylxylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases.

scientific article published on 23 January 2006

Structure-function analyses generate novel specificities to assemble the components of multi-enzyme bacterial cellulosome complexes

scientific article published on 24 January 2018

T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis.

scientific article published on 22 June 2016

Target highlights from the first post-PSI CASP experiment (CASP12, May-August 2016).

scientific article published on 28 September 2017

The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans

scientific article

The X6 "thermostabilizing" domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain

scientific article

The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities.

scientific article published on 08 March 2004

The family 6 Carbohydrate Binding Module (CtCBM6) of glucuronoxylanase (CtXynGH30) of Clostridium thermocellum binds decorated and undecorated xylans through cleft A.

scientific article published on 07 April 2015

The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities.

scientific article

The mechanisms by which family 10 glycoside hydrolases bind decorated substrates

scientific article

The membrane-bound alpha-glucuronidase from Pseudomonas cellulosa hydrolyzes 4-O-methyl-D-glucuronoxylooligosaccharides but not 4-O-methyl-D-glucuronoxylan.

scientific article published on September 2002

The prion-related protein (testis-specific) gene (PRNT) is highly polymorphic in Portuguese sheep

scientific article published on 05 November 2015

The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition

scientific article

Thermostable recombinant β-(1→4)-mannanase from C. thermocellum: biochemical characterization and manno-oligosaccharides production.

scientific article

Variation in levels of non-starch polysaccharides and endogenous endo-1,4-β-xylanases affect the nutritive value of wheat for poultry.

scientific article published on 9 February 2018

Xylo-oligosaccharides display a prebiotic activity when used to supplement wheat or corn-based diets for broilers

scientific article published on 01 December 2018