List of works - Lucília Domingues

A comparative study of recombinant and native frutalin binding to human prostate tissues

scientific article

Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells

scientific article published in March 2001

Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus

scientific article published on 6 June 2007

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

scientific article published on 06 October 2015

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

scientific article published on 17 July 2019

Bacterial cellulose modified using recombinant proteins to improve neuronal and mesenchymal cell adhesion ⬇️

scientific article published on January 23, 2012

Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system ⬇️

scientific article published on September 9, 2010

Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

scientific article published in January 2015

Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2

scientific article published on 06 September 2011

Cellulase recycling in biorefineries--is it possible?

scientific article published on 29 March 2015

Characterization and genome sequencing of a Citrobacter freundii phage CfP1 harboring a lysin active against multidrug-resistant isolates.

scientific article published on 28 September 2016

Characterization of the Ashbya gossypii secreted N-glycome and genomic insights into its N-glycosylation pathway

scientific article

Combined alkali and hydrothermal pretreatments for oat straw valorization within a biorefinery concept

scientific article published on 24 August 2016

Comparative transcriptome analysis between original and evolved recombinant lactose-consuming Saccharomyces cerevisiae strains.

scientific article published in December 2008

Construction of a flocculent Saccharomyces cerevisiae fermenting lactose

scientific article published on 01 May 1999

Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger beta-galactosidase

scientific article published on 19 February 2002

Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase

scientific article published on 01 July 2000

Contamination of a high-cell-density continuous bioreactor

scientific article published on 5 June 2000

Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

scientific article published on 20 September 1999

Contribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors

scientific article published on 7 May 2015

Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering

scientific article published on 2 May 2014

Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells.

scientific article published on November 2011

Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings.

scientific article published on 16 April 2018

Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence

scientific article

Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production

scientific article

Differentiation of human pre-adipocytes by recombinant adiponectin

scientific article published on 26 January 2008

Effect of hemicellulose liquid phase on the enzymatic hydrolysis of autohydrolyzed Eucalyptus globulus wood

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

scientific article published on 01 December 2020

Enhanced heterologous protein production in Pichia pastoris under increased air pressure.

scientific article published on 6 August 2014

Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum

scientific article

Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum.

scientific article

Evaluation of strategies for second generation bioethanol production from fast growing biomass Paulownia within a biorefinery scheme

Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii

scientific article published on 27 April 2010

Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris.

scientific article

Factors affecting extraction of adsorbed wine volatile compounds and wood extractives from used oak wood

scientific article published on 13 May 2019

Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation

scientific article published on 10 June 2010

Fermentation of high concentrations of lactose to ethanol by engineered flocculent Saccharomyces cerevisiae

scientific article published on 25 June 2008

Fractionation of Eucalyptus globulus Wood by Glycerol–Water Pretreatment: Optimization and Modeling

article published in 2013

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

scientific article

Genome-wide metabolic re-annotation of Ashbya gossypii: new insights into its metabolism through a comparative analysis with Saccharomyces cerevisiae and Kluyveromyces lactis

scientific article

Genome-wide screening of Saccharomyces cerevisiae genes required to foster tolerance towards industrial wheat straw hydrolysates.

scientific article published on 7 October 2014

Guidelines to reach high-quality purified recombinant proteins

scientific article published on 18 November 2017

HAA1 and PRS3 overexpression boosts yeast tolerance towards acetic acid improving xylose or glucose consumption: unravelling the underlying mechanisms.

scientific article published on 2 April 2018

High-level expression of Aspergillus niger b-galactosidase in Ashbya gossypii

scientific article

Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations.

scientific article published on 9 December 2011

Improving bacterial cellulose for blood vessel replacement: Functionalization with a chimeric protein containing a cellulose-binding module and an adhesion peptide

scientific article published on 14 May 2010

Improving the affinity of fibroblasts for bacterial cellulose using carbohydrate-binding modules fused to RGD.

scientific article published on January 2010

Industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass

scientific article published on 20 March 2014

Integral valorization of vine pruning residue by sequential autohydrolysis stages

Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings

article

Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: Importance of yeast chassis linked to process conditions

scientific article published on 11 December 2016

Intensifying ethanol production from brewer's spent grain waste: Use of whole slurry at high solid loadings

scientific article published on 10 June 2019

Investigation of protein secretion and secretion stress in Ashbya gossypii

scientific article

Kinetics of lactose fermentation using a recombinant Saccharomyces cerevisiae strain.

scientific article published in August 2006

Light exposure during growth increases riboflavin production, ROS accumulation and DNA damage in Ashbya gossypii riboflavin-overproducing strains

article

Lignocellulosic bioethanol production with revalorization of low-cost agroindustrial by-products as nutritional supplements

article

Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones

scientific article published on 28 March 2019

Metabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation

article by Aloia Romaní et al published March 2015 in Bioresource Technology

Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation.

scientific article

Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

scientific article published on 26 August 2019

Molecular and Functional Characterization of an Invertase Secreted by Ashbya gossypii

scientific article published on 01 June 2014

Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions

scientific article published in 2018

New biotechnological applications for Ashbya gossypii: Challenges and perspectives

scientific article published on 28 October 2016

Nutritional requirements and strain heterogeneity in Ashbya gossypii

scientific article published on 5 December 2011

On the track for an efficient detection of Escherichia coli in water: A review on PCR-based methods.

scientific article published on 23 December 2014

Optimization of low-cost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs

scientific article published on 02 June 2010

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii

scientific article published on 24 December 2018

Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation–sedimentation

scientific article published on 24 December 2011

Production of a Distilled Spirit Using Cassava Flour as Raw Material: Chemical Characterization and Sensory Profile

scientific article published on 15 July 2020

Production of fermented cheese whey-based beverage using kefir grains as starter culture: evaluation of morphological and microbial variations.

scientific article

Production ofβ-galactosidase from recombinantSaccharomyces cerevisiae grown on lactose

article

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production.

scientific article published on 17 May 2016

RAPD/SCAR Approaches for Identification of Adulterant Breeds' Milk in Dairy Products

scientific article

Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii

scientific article published on 15 April 2013

Recent trends on seaweed fractionation for liquid biofuels production

scientific article published on 14 December 2019

Recombinant CBM-fusion technology - Applications overview.

scientific article

Recombinant expression and purification of the antimicrobial peptide magainin-2.

scientific article

Recombinant lectins: an array of tailor-made glycan-interaction biosynthetic tools.

scientific article published on 24 April 2012

Recombinant production of plant lectins in microbial systems for biomedical application - the frutalin case study

scientific article

Relationships between hydrodynamics and rheology of flocculating yeast suspensions in a high-cell-density airlift bioreactor.

scientific article published in February 2005

Robust industrial Saccharomyces cerevisiae strains for very high gravity bio-ethanol fermentations

scientific article published on 02 May 2011

SLMP53-2 Restores Wild-Type-Like Function to Mutant p53 through Hsp70: Promising Activity in Hepatocellular Carcinoma

scientific article published on 10 August 2019

Selection of Saccharomyces cerevisiae strains for efficient very high gravity bio-ethanol fermentation processes.

scientific article

Simultaneous Saccharification and Fermentation of Hydrothermal Pretreated Lignocellulosic Biomass: Evaluation of Process Performance Under Multiple Stress Conditions

Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

scientific article published in 2023

Synthesis of Fusion Genes for Cloning by Megaprimer-Based PCR.

scientific article published in January 2017

The Fh8 tag: a fusion partner for simple and cost-effective protein purification in Escherichia coli.

scientific article published on 29 September 2013

The inhibitory effect of an RGD-human chitin-binding domain fusion protein on the adhesion of fibroblasts to reacetylated chitosan films.

scientific article

The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology.

scientific article published on 20 November 2012

Third generation bioethanol from invasive macroalgae Sargassum muticum using autohydrolysis pretreatment as first step of a biorefinery

scientific article published in 2019

Validation of a LLME/GC-MS Methodology for Quantification of Volatile Compounds in Fermented Beverages

scientific article published on 31 January 2020

Valorization of Eucalyptus wood by glycerol-organosolv pretreatment within the biorefinery concept: An integrated and intensified approach

Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling

scientific article published on 2 June 2016

Vinegar production from fruit concentrates: effect on volatile composition and antioxidant activity.

scientific article published on 23 September 2017

Virtual laboratories in (bio)chemical engineering education

WITHDRAWN: Technological trends, global market, and challenges of bio-ethanol production

retracted scientific article published on 01 July 2010

Wound healing activity of the human antimicrobial peptide LL37

scientific article published on 13 June 2011

Xylose fermentation efficiency of industrial yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways

scientific article published on 28 January 2019

cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli.

scientific article

List of works by Lucília Domingues

A comparative study of recombinant and native frutalin binding to human prostate tissues

Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells

Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

BSA-based sample clean-up columns for ochratoxin A determination in wine: Method development and validation

Bacterial cellulose modified using recombinant proteins to improve neuronal and mesenchymal cell adhesion ⬇️

Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system ⬇️

Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2

Cellulase recycling in biorefineries--is it possible?

Characterization and genome sequencing of a Citrobacter freundii phage CfP1 harboring a lysin active against multidrug-resistant isolates.

Characterization of the Ashbya gossypii secreted N-glycome and genomic insights into its N-glycosylation pathway

Combined alkali and hydrothermal pretreatments for oat straw valorization within a biorefinery concept

Comparative transcriptome analysis between original and evolved recombinant lactose-consuming Saccharomyces cerevisiae strains.

Construction of a flocculent Saccharomyces cerevisiae fermenting lactose

Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger beta-galactosidase

Construction of a flocculent brewer's yeast strain secreting Aspergillus niger β-galactosidase

Contamination of a high-cell-density continuous bioreactor

Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

Contribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors

Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering

Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells.

Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings.

Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence

Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production

Differentiation of human pre-adipocytes by recombinant adiponectin

Effect of hemicellulose liquid phase on the enzymatic hydrolysis of autohydrolyzed Eucalyptus globulus wood

Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production

Enhanced heterologous protein production in Pichia pastoris under increased air pressure.

Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum

Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum.

Evaluation of strategies for second generation bioethanol production from fast growing biomass Paulownia within a biorefinery scheme

Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii

Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris.

Factors affecting extraction of adsorbed wine volatile compounds and wood extractives from used oak wood

Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation

Fermentation of high concentrations of lactose to ethanol by engineered flocculent Saccharomyces cerevisiae

Fractionation of Eucalyptus globulus Wood by Glycerol–Water Pretreatment: Optimization and Modeling

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

Genome-wide metabolic re-annotation of Ashbya gossypii: new insights into its metabolism through a comparative analysis with Saccharomyces cerevisiae and Kluyveromyces lactis

Genome-wide screening of Saccharomyces cerevisiae genes required to foster tolerance towards industrial wheat straw hydrolysates.

Guidelines to reach high-quality purified recombinant proteins

HAA1 and PRS3 overexpression boosts yeast tolerance towards acetic acid improving xylose or glucose consumption: unravelling the underlying mechanisms.

High-level expression of Aspergillus niger b-galactosidase in Ashbya gossypii

Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations.

Improving bacterial cellulose for blood vessel replacement: Functionalization with a chimeric protein containing a cellulose-binding module and an adhesion peptide

Improving the affinity of fibroblasts for bacterial cellulose using carbohydrate-binding modules fused to RGD.

Industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass

Integral valorization of vine pruning residue by sequential autohydrolysis stages

Integrated approach for effective bioethanol production using whole slurry from autohydrolyzed Eucalyptus globulus wood at high-solid loadings

Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: Importance of yeast chassis linked to process conditions

Intensifying ethanol production from brewer's spent grain waste: Use of whole slurry at high solid loadings

Investigation of protein secretion and secretion stress in Ashbya gossypii

Kinetics of lactose fermentation using a recombinant Saccharomyces cerevisiae strain.

Light exposure during growth increases riboflavin production, ROS accumulation and DNA damage in Ashbya gossypii riboflavin-overproducing strains

Lignocellulosic bioethanol production with revalorization of low-cost agroindustrial by-products as nutritional supplements

Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones

Metabolic engineering of Saccharomyces cerevisiae ethanol strains PE-2 and CAT-1 for efficient lignocellulosic fermentation

Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation.

Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

Molecular and Functional Characterization of an Invertase Secreted by Ashbya gossypii

Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions

New biotechnological applications for Ashbya gossypii: Challenges and perspectives

Nutritional requirements and strain heterogeneity in Ashbya gossypii

On the track for an efficient detection of Escherichia coli in water: A review on PCR-based methods.

Optimization of low-cost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii

Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation–sedimentation

Production of a Distilled Spirit Using Cassava Flour as Raw Material: Chemical Characterization and Sensory Profile

Production of fermented cheese whey-based beverage using kefir grains as starter culture: evaluation of morphological and microbial variations.

Production ofβ-galactosidase from recombinantSaccharomyces cerevisiae grown on lactose

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production.

RAPD/SCAR Approaches for Identification of Adulterant Breeds' Milk in Dairy Products

Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii

Recent trends on seaweed fractionation for liquid biofuels production

Recombinant CBM-fusion technology - Applications overview.

Recombinant expression and purification of the antimicrobial peptide magainin-2.

Recombinant lectins: an array of tailor-made glycan-interaction biosynthetic tools.

Recombinant production of plant lectins in microbial systems for biomedical application - the frutalin case study