List of works by Richard T. Wyatt

A Blueprint for HIV Vaccine Discovery

scientific article

A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly ⬇️

scientific article published on October 15, 2010

A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding

scientific journal article

A method for identification of HIV gp140 binding memory B cells in human blood

scientific article

A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples

scientific article

A tyrosine-rich region in the N terminus of CCR5 is important for human immunodeficiency virus type 1 entry and mediates an association between gp120 and CCR5

scientific article published on February 1998

Access of antibody molecules to the conserved coreceptor binding site on glycoprotein gp120 is sterically restricted on primary human immunodeficiency virus type 1

scientific article

Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease

scientific article published on 15 July 2019

An HIV-1 Env-Antibody Complex Focuses Antibody Responses to Conserved Neutralizing Epitopes.

scientific article published on 10 October 2016

An aptamer that neutralizes R5 strains of human immunodeficiency virus type 1 blocks gp120-CCR5 interaction

scientific article

Analysis of neutralization specificities in polyclonal sera derived from human immunodeficiency virus type 1-infected individuals

scientific article

Analysis of the human immunodeficiency virus type 1 gp41 membrane proximal external region arrayed on hepatitis B surface antigen particles

scientific article published on 26 December 2007

Analysis of the interaction of antibodies with a conserved enzymatically deglycosylated core of the HIV type 1 envelope glycoprotein 120

scientific article published on 01 February 1998

Analysis of the interaction of the human immunodeficiency virus type 1 gp120 envelope glycoprotein with the gp41 transmembrane glycoprotein.

scientific article published on December 1997

Analysis of the neutralizing antibody response elicited in rabbits by repeated inoculation with trimeric HIV-1 envelope glycoproteins.

scientific article published in January 2005

Anti-HIV B Cell lines as candidate vaccine biosensors

scientific article published on 12 October 2012

Antibody 17b binding at the coreceptor site weakens the kinetics of the interaction of envelope glycoprotein gp120 with CD4.

scientific article published on February 2001

Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs

scientific article published in 2019

B cell recognition of the conserved HIV-1 co-receptor binding site is altered by endogenous primate CD4.

scientific article

B cells from knock-in mice expressing broadly neutralizing HIV antibody b12 carry an innocuous B cell receptor responsive to HIV vaccine candidates

scientific article published on 12 August 2013

BLyS-mediated modulation of naive B cell subsets impacts HIV Env-induced antibody responses

scientific article

Biochemical and immunogenic characterization of soluble human immunodeficiency virus type 1 envelope glycoprotein trimers expressed by semliki forest virus

scientific article

Biochemically defined HIV-1 envelope glycoprotein variant immunogens display differential binding and neutralizing specificities to the CD4-binding site

scientific article published on 13 December 2011

Breadth of human immunodeficiency virus-specific neutralizing activity in sera: clustering analysis and association with clinical variables

scientific article

Broad HIV-1 neutralization mediated by CD4-binding site antibodies

scientific article published on 26 August 2007

Broad and potent HIV-1 neutralization by a human antibody that binds the gp41-gp120 interface

scientific article

Broad and potent neutralization of HIV-1 by a gp41-specific human antibody

scientific article

Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals

scientific article

CD4-independent binding of SIV gp120 to rhesus CCR5.

scientific article

CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5.

scientific article published on November 1996

CD4-mimetic small molecules sensitize human immunodeficiency virus to vaccine-elicited antibodies

scientific article

CTA1-DD adjuvant promotes strong immunity against human immunodeficiency virus type 1 envelope glycoproteins following mucosal immunization

scientific article published in December 2008

Calcium Phosphate Nanoparticle-Based Vaccines as a Platform for Improvement of HIV-1 Env Antibody Responses by Intrastructural Help

scientific article published on 27 September 2019

Characterization of antibody responses elicited by human immunodeficiency virus type 1 primary isolate trimeric and monomeric envelope glycoproteins in selected adjuvants

scientific article

Characterization of antibody responses to purified HIV-1 gp120 glycoproteins fused with the molecular adjuvant C3d

scientific article published on 01 September 2005

Characterization of human immunodeficiency virus type 1 monomeric and trimeric gp120 glycoproteins stabilized in the CD4-bound state: antigenicity, biophysics, and immunogenicity ⬇️

scientific article published on 14 March 2007

Characterization of stable, soluble trimers containing complete ectodomains of human immunodeficiency virus type 1 envelope glycoproteins

scientific article

Chemical cross-linking of HIV-1 Env for direct TLR7/8 ligand conjugation compromises recognition of conserved antigenic determinants

scientific article published on 15 August 2013

Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies.

scientific article

Cleavage-independent HIV-1 Env trimers engineered as soluble native spike mimetics for vaccine design

scientific article published on 16 April 2015

Conformation of the HIV-1 gp 120 envelope glycoprotein

scientific article published on January 1996

Conformational changes of gp120 in epitopes near the CCR5 binding site are induced by CD4 and a CD4 miniprotein mimetic

scientific article

Correction for Chakrabarti et al., Robust Neutralizing Antibodies Elicited by HIV-1 JRFL Envelope Glycoprotein Trimers in Nonhuman Primates.

scientific article published on 16 December 2014

Correction for Ingale et al., Hyperglycosylated stable core immunogens designed to present the CD4 binding site are preferentially recognized by broadly neutralizing antibodies

scientific article published on June 2015

Correction for Khan et al., "Targeting the HIV-1 Spike and Coreceptor with Bi- and Trispecific Antibodies for Single-Component Broad Inhibition of Entry".

scientific article published on 15 May 2019

Correction: Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability

scientific article published on 24 May 2016

Corrigendum to “A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples”

scholarly article published in Journal of Immunological Methods

Covalent Linkage of HIV-1 Trimers to Synthetic Liposomes Elicits Improved B Cell and Antibody Responses

scientific article published on 07 June 2017

Dense Array of Spikes on HIV-1 Virion Particles

scientific article published on 26 April 2017

Design clues from functional constraints and broadly neutralizing antibodies

Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer

scientific article published in Nature Communications

Direct antibody access to the HIV-1 membrane-proximal external region positively correlates with neutralization sensitivity

scientific article published on 8 June 2011

Diverse antibody genetic and recognition properties revealed following HIV-1 envelope glycoprotein immunization

scientific article published on 11 May 2015

Elicitation of Neutralizing Antibodies Targeting the V2 Apex of the HIV Envelope Trimer in a Wild-Type Animal Model

scientific article published on October 2017

Elicitation of Neutralizing Antibodies Targeting the V2 Apex of the HIV Envelope Trimer in a Wild-Type Animal Model.

scientific article published on 28 January 2018

Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches

scientific article

Elicitation of structure-specific antibodies by epitope scaffolds

scientific article

Energetics of the HIV gp120-CD4 binding reaction

scientific article

Engineered mice and B cell lines expressing broadly neutralizing antibodies and their unmutated precursors: tools for HIV vaccinology.

scientific article published on 13 September 2012

Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor

scientific article

Evolution of B cell analysis and Env trimer redesign

scientific article

Extensive dissemination and intraclonal maturation of HIV Env vaccine-induced B cell responses

scientific article published on 01 February 2020

Factors limiting the immunogenicity of HIV-1 gp120 envelope glycoproteins

scientific article published on 01 December 2004

Focused dampening of antibody response to the immunodominant variable loops by engineered soluble gp140.

scientific article published on February 2008

Frequency and phenotype of human immunodeficiency virus envelope-specific B cells from patients with broadly cross-neutralizing antibodies

scientific article published on 15 October 2008

Glutaraldehyde Cross-linking of HIV-1 Env Trimers Skews the Antibody Subclass Response in Mice

scientific article published on 27 November 2017

Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein

scientific article

HIV type 1 Env precursor cleavage state affects recognition by both neutralizing and nonneutralizing gp41 antibodies

scientific article

HIV vaccine design and the neutralizing antibody problem

scientific article published in March 2004

HIV-1 Cross-reactive Primary Virus Neutralizing Antibody Response Elicited by Immunization in Non-human Primate

scientific article

HIV-1 Vaccine-elicited Antibodies Reverted to Their Inferred Naive Germline Reveal Associations between Binding Affinity and in vivo Activation

scientific article published on 16 February 2016

HIV-1 envelope glycoprotein trimer immunogenicity elicited in the presence of human CD4 alters the neutralization profile

scientific article

HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites

scientific article (publication date: 12 December 2002)

HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelope glycoproteins

scientific article

HIV-1 neutralizing antibodies display dual specificity for the primary and coreceptor binding sites and preferential recognition of fully-cleaved Env.

scientific article published on 13 September 2012

HIV-1 receptor binding site-directed antibodies using a VH1-2 gene segment orthologue are activated by Env trimer immunization

scientific article

Heterologous epitope-scaffold prime:boosting immuno-focuses B cell responses to the HIV-1 gp41 2F5 neutralization determinant

scientific article

High-Density Array of Well-Ordered HIV-1 Spikes on Synthetic Liposomal Nanoparticles Efficiently Activate B Cells.

scientific article published on 18 May 2016

High-Resolution Longitudinal Study of HIV-1 Env Vaccine-Elicited B Cell Responses to the Virus Primary Receptor Binding Site Reveals Affinity Maturation and Clonal Persistence.

scientific article published on 21 March 2016

High-resolution definition of vaccine-elicited B cell responses against the HIV primary receptor binding site

scientific article published on July 2012

Highly stable trimers formed by human immunodeficiency virus type 1 envelope glycoproteins fused with the trimeric motif of T4 bacteriophage fibritin

scientific article

Human immunodeficiency virus type 1 env trimer immunization of macaques and impact of priming with viral vector or stabilized core protein.

scientific article

Human plasmacytoid dendritic cells efficiently capture HIV-1 envelope glycoproteins via CD4 for antigen presentation

scientific article published on 31 May 2013

Hyperglycosylated stable core immunogens designed to present the CD4 binding site are preferentially recognized by broadly neutralizing antibodies

scientific article

Immunization with recombinant HLA classes I and II, HIV-1 gp140, and SIV p27 elicits protection against heterologous SHIV infection in rhesus macaques.

scientific article

Immunization with wild-type or CD4-binding-defective HIV-1 Env trimers reduces viremia equivalently following heterologous challenge with simian-human immunodeficiency virus

scientific article

Immunogenicity of DNA vaccines expressing human immunodeficiency virus type 1 envelope glycoprotein with and without deletions in the V1/2 and V3 regions

scientific article published in January 1998

Immunogenicity of HIV-1 envelope glycoprotein oligomers

scientific article published on September 2009

Improved elicitation of neutralizing antibodies against primary human immunodeficiency viruses by soluble stabilized envelope glycoprotein trimers

scientific article

Independent expansion of epitope-specific plasma cell responses upon HIV-1 envelope glycoprotein immunization.

scientific article published on 5 June 2013

Influence of novel CD4 binding-defective HIV-1 envelope glycoprotein immunogens on neutralizing antibody and T-cell responses in nonhuman primates

scientific article

Interactions among HIV gp120, CD4, and CXCR4: dependence on CD4 expression level, gp120 viral origin, conservation of the gp120 COOH- and NH2-termini and V1/V2 and V3 loops, and sensitivity to neutralizing antibodies.

scientific article published on September 1998

Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 epitopes induced by receptor binding

scientific article published in September 1995

Key gp120 Glycans Pose Roadblocks to the Rapid Development of VRC01-Class Antibodies in an HIV-1-Infected Chinese Donor

scientific article published on 5 April 2016

Ligand accessibility to the HIV-1 Env co-receptor binding site can occur prior to CD4 engagement and is independent of viral tier category.

scientific article published on 20 April 2018

Mechanism of human immunodeficiency virus type 1 resistance to monoclonal antibody B12 that effectively targets the site of CD4 attachment

scientific article published on 19 August 2009

Mechanism of neutralization by the broadly neutralizing HIV-1 monoclonal antibody VRC01.

scientific article

Migration of antigen-specific T cells away from CXCR4-binding human immunodeficiency virus type 1 gp120.

scientific article published on May 2004

Modifications that stabilize human immunodeficiency virus envelope glycoprotein trimers in solution

scientific article

Multiple Antibody Lineages in One Donor Target the Glycan-V3 Supersite of the HIV-1 Envelope Glycoprotein and Display a Preference for Quaternary Binding

scientific article published on 21 September 2016

Mutagenic stabilization and/or disruption of a CD4-bound state reveals distinct conformations of the human immunodeficiency virus type 1 gp120 envelope glycoprotein

scientific article

OA05-05. Impact of in vivo CD4 binding during HIV-1 Env trimer immunizations of rhesus macaques.

scientific article published on 22 October 2009

Oligomeric modeling and electrostatic analysis of the gp120 envelope glycoprotein of human immunodeficiency virus

scientific article

Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization

scientific article published on 01 December 2019

P05-07. Evaluation of peripheral and bone marrow B cell responses in rhesus macaques after immunization with soluble HIV-1 gp140 trimers.

scientific article

P09-05. Mechanism of HIV-1 resistance to a monoclonal antibody that effectively targets the site of CD4 attachment

article

P09-13. Structure of HIV-1 gp41 interactive region: layered architecture and basis of conformational mobility

article

P12-12. Analysis of antibody and B cell responses following inoculation with computationally designed HIV-1 2F5 epitope scaffold proteins

scientific article

P19-08. Immunisation with recombinant HLA class I and II, HIV-1gp140 and SIVp27 antigens elicits protection against SHIV-SF162P4 infection in rhesus macaques.

scientific article published on 22 October 2009

PGV04, an HIV-1 gp120 CD4 binding site antibody, is broad and potent in neutralization but does not induce conformational changes characteristic of CD4.

scientific article published on 15 February 2012

Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach

scientific article published on May 2017

Phosphoserine acidic cluster motifs bind distinct basic regions on the μ subunits of clathrin adaptor protein complexes

scientific article published in Journal of Biological Chemistry

Primate immune responses to HIV-1 Env formulated in the saponin-based adjuvant AbISCO-100 in the presence or absence of TLR9 co-stimulation

scientific article

Priming with DNA encoding functional trimers and boosting with soluble trimeric protein elicited tier 2 neutralizing antibodies in non-human primates

scientific article published on 13 September 2012

Probability analysis of variational crystallization and its application to gp120, the exterior envelope glycoprotein of type 1 human immunodeficiency virus (HIV-1).

scientific article published in February 1999

Probing the structure of the human immunodeficiency virus surface glycoprotein gp120 with a panel of monoclonal antibodies

scientific article published on January 1994

Proof of principle for epitope-focused vaccine design

scientific article

Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1

scientific article (publication date: 13 August 2010)

Relationship between antibody 2F5 neutralization of HIV-1 and hydrophobicity of its heavy chain third complementarity-determining region

scientific article

Relationship of the human immunodeficiency virus type 1 gp120 third variable loop to a component of the CD4 binding site in the fourth conserved region ⬇️

scientific article published on December 1, 1992

Rhesus Macaque B-Cell Responses to an HIV-1 Trimer Vaccine Revealed by Unbiased Longitudinal Repertoire Analysis

scientific article

Robust neutralizing antibodies elicited by HIV-1 JRFL envelope glycoprotein trimers in nonhuman primates

scientific article published on 25 September 2013

Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity

scientific article published on 01 March 2020

Scorpion-toxin mimics of CD4 in complex with human immunodeficiency virus gp120 crystal structures, molecular mimicry, and neutralization breadth

scientific article

Selection pressure on HIV-1 envelope by broadly neutralizing antibodies to the conserved CD4-binding site

scientific article

Selective expansion of HIV-1 envelope glycoprotein-specific B cell subsets recognizing distinct structural elements following immunization

scientific article

Selective interactions of polyanions with basic surfaces on human immunodeficiency virus type 1 gp120

scientific article

Selective recognition of oligomeric HIV-1 primary isolate envelope glycoproteins by potently neutralizing ligands requires efficient precursor cleavage.

scientific article published on February 2005

Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization

scientific article published on 12 March 2014

Small-molecule CD4 mimics interact with a highly conserved pocket on HIV-1 gp120.

scientific article

Solid-phase proteoliposomes containing human immunodeficiency virus envelope glycoproteins

scientific article

Soluble HIV-1 Env trimers in adjuvant elicit potent and diverse functional B cell responses in primates

scientific article

Soluble HIV-1 Env trimers in adjuvant elicit potent and diverse functional B cell responses in primates

Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis

scientific article published on August 2005

Stabilization of human immunodeficiency virus type 1 envelope glycoprotein trimers by disulfide bonds introduced into the gp41 glycoprotein ectodomain

scientific article

Structural Basis of Immune Evasion at the Site of CD4 Attachment on HIV-1 gp120

scientific article

Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120

scientific article

Structural definition of a conserved neutralization epitope on HIV-1 gp120

scientific article

Structure and mechanistic analysis of the anti-human immunodeficiency virus type 1 antibody 2F5 in complex with its gp41 epitope

scientific article

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9

scientific article

Structure of HIV-1 gp120 with gp41-interactive region reveals layered envelope architecture and basis of conformational mobility

scientific article

Structure of a V3-containing HIV-1 gp120 core

scientific article

Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.

scientific article

Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody

scientific article

Structure-Guided Redesign Improves NFL HIV Env Trimer Integrity and Identifies an Inter-Protomer Disulfide Permitting Post-Expression Cleavage

scientific article published on 17 July 2018

Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers

scientific article published on 30 December 2015

Structure-activity relationships in the binding of chemically derivatized CD4 to gp120 from human immunodeficiency virus

scientific article published on 6 September 2007

Structure-based stabilization of HIV-1 gp120 enhances humoral immune responses to the induced co-receptor binding site

scientific article (publication date: May 2009)

Structure-based, targeted deglycosylation of HIV-1 gp120 and effects on neutralization sensitivity and antibody recognition.

scientific article published in September 2003

Structure-guided alterations of the gp41-directed HIV-1 broadly neutralizing antibody 2F5 reveal new properties regarding its neutralizing function

scientific article

Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates

scientific article

Structures of the CCR5 N Terminus and of a Tyrosine-Sulfated Antibody with HIV-1 gp120 and CD4

scientific article

Studies with monoclonal antibodies to the V3 region of HIV-1 gp120 reveal limitations to the utility of solid-phase peptide binding assays

scientific article published in April 1994

Sudan Ebolavirus VP35-NP Crystal Structure Reveals a Potential Target for Pan-Filovirus Treatment

scientific article published on 23 July 2019

Synthetic multivalent V3 glycopeptides display enhanced recognition by glycan-dependent HIV-1 broadly neutralizing antibodies

scientific article published on 3 May 2017

Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability

scientific article

Targeted N-glycan deletion at the receptor-binding site retains HIV Env NFL trimer integrity and accelerates the elicited antibody response

scientific article published on 13 September 2017

Targeting the HIV-1 Spike and Coreceptor with Bi- and Trispecific Antibodies for Single-Component Broad Inhibition of Entry

scholarly article by Salar N Khan et al published 15 September 2018 in Journal of Virology

The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization

article

The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens

scientific article

The antigenic structure of the HIV gp120 envelope glycoprotein

scientific article

The challenges of eliciting neutralizing antibodies to HIV-1 and to influenza virus.

scientific article

The envelope glycoprotein ectodomains determine the efficiency of CD4+ T lymphocyte depletion in simian-human immunodeficiency virus-infected macaques

scientific article

Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies

scientific article

Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry

scientific article published in March 1999

Tyrosine-sulfate isosteres of CCR5 N-terminus as tools for studying HIV-1 entry

scientific article

Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability

scientific article published on 12 November 2019

Vaccine-Elicited Tier 2 HIV-1 Neutralizing Antibodies Bind to Quaternary Epitopes Involving Glycan-Deficient Patches Proximal to the CD4 Binding Site

scientific article

Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers

scholarly article by Matthias G Pauthner et al published 15 January 2019 in Immunity

Vaccine-elicited primate antibodies use a distinct approach to the HIV-1 primary receptor binding site informing vaccine redesign

scientific article

Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop

scientific article published on May 2017

Well-ordered trimeric HIV-1 subtype B and C soluble spike mimetics generated by negative selection display native-like properties

scientific article published on 8 January 2015

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