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List of works by Klaas Hellingwerf

A Kdp-like, high-affinity, K+-translocating ATPase is expressed during growth of Rhodobacter sphaeroides in low potassium media

scholarly article by Tjakko Abee published in October 1992

A base-catalyzed mechanism for dark state recovery in the Avena sativa phototropin-1 LOV2 domain

scientific article published on 21 February 2007

A kinetic model of catabolic adaptation and protein reprofiling in Saccharomyces cerevisiae during temperature shifts

scientific article published in February 2014

A network of net-workers: report of the Euresco conference on 'Bacterial Neural Networks' held at San Feliu (Spain) from 8 to 14 May 2004

scientific article published on 01 October 2004

Activation of the General Stress Response of Bacillus subtilis by Visible Light

scientific article published on 18 July 2015

Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation

scientific article published on August 2005

Algal photosynthesis as the primary driver for a sustainable development in energy, feed, and food production

scientific article

Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth

scientific article published on 13 February 2018

All Three Endogenous Quinone Species of Escherichia coli Are Involved in Controlling the Activity of the Aerobic/Anaerobic Response Regulator ArcA.

scientific article published on 7 September 2016

Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light

scientific article published on 27 July 2018

Assessment of heat resistance of bacterial spores from food product isolates by fluorescence monitoring of dipicolinic acid release

scientific article published on July 2005

Bacterial observations: a rudimentary form of intelligence?

scientific article published on 01 April 2005

Bacteriorhodopsin in liposomes: Quantitative evaluation of ΔpH changes induced by variations of light intensity and conductivity parameters

article published in 1981

Binding of Hydrogen-Citrate to Photoactive Yellow Protein Is Affected by the Structural Changes Related to Signaling State Formation

article

Binding, tuning and mechanical function of the 4-hydroxy-cinnamic acid chromophore in photoactive yellow protein

scientific article published on 19 March 2007

Blue light activates the sigmaB-dependent stress response of Bacillus subtilis via YtvA.

scientific article published on September 2006

Blue light induces major changes in the gene expression profile of the cyanobacterium Synechocystis sp. PCC 6803

scientific article published on 06 March 2020

Blue light reduces photosynthetic efficiency of cyanobacteria through an imbalance between photosystems I and II

scientific article published on 19 July 2018

Blue-light-dependent inhibition of twitching motility in Acinetobacter baylyi ADP1: additive involvement of three BLUF-domain-containing proteins.

scientific article

Carbon Monoxide Gas Is Not Inert, but Global, in Its Consequences for Bacterial Gene Expression, Iron Acquisition, and Antibiotic Resistance

scientific article published on 24 February 2016

Carbon sink removal: Increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant.

scientific article published on 21 May 2014

Challenges in the Application of Synthetic Biology Toward Synthesis of Commodity Products by Cyanobacteria via "Direct Conversion"

scholarly article by Wei Du et al published 2018 in Advances in Experimental Medicine and Biology

Changes at the KinA PAS-A dimerization interface influence histidine kinase function

scientific article

Chapter 6 Photoactive yellow protein, a photoreceptor from purple bacteria

scholarly article published 2001

Characterization of photocycle intermediates in crystalline photoactive yellow protein

scientific article published on 01 August 2003

Characterization of the primary photochemistry of proteorhodopsin with femtosecond spectroscopy

scientific article published on 30 January 2008

Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae.

scientific article

Chirality Matters: Synthesis and Consumption of the d-Enantiomer of Lactic Acid by Synechocystis sp. Strain PCC6803.

scientific article published on 18 December 2015

Chromophore composition of a heterologously expressed BLUF-domain

scientific article published on 4 November 2004

Combining retinal-based and chlorophyll-based (oxygenic) photosynthesis: Proteorhodopsin expression increases growth rate and fitness of a ∆PSI strain of Synechocystis sp. PCC6803

scientific article published on 14 November 2018

Comparative analysis of transcriptome and fitness profiles reveals general and condition-specific cellular functions involved in adaptation to environmental change in Saccharomyces cerevisiae.

scientific article published on 9 August 2010

Comparison of the Photosynthetic Yield of Cyanobacteria and Green Algae: Different Methods Give Different Answers

scientific article published on 22 September 2015

Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein

scientific article published on 25 August 2020

Conformational heterogeneity and propagation of structural changes in the LOV2/Jalpha domain from Avena sativa phototropin 1 as recorded by temperature-dependent FTIR spectroscopy

scientific article published in July 2009

Contrasting the excited-state dynamics of the photoactive yellow protein chromophore: protein versus solvent environments

scientific article

Controlled reduction of the humidity induces a shortcut recovery reaction in the photocycle of photoactive yellow protein

scientific article published in June 2005

Cooperativity in signal transfer through the Uhp system of Escherichia coli

scientific article published on August 2002

Corrigendum: All Three Endogenous Quinone Species of Escherichia coli Are Involved in Controlling the Activity of the Aerobic/Anaerobic Response Regulator ArcA

scientific article published on 15 July 2020

Corrigendum: Synechocystis: not just a plug-bug for CO2, but a green E. coli

scientific article published on 13 April 2016

Culturing Synechocystis sp. Strain PCC 6803 with N2 and CO2 in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs

scientific article

Deletion of sll1541 in Synechocystis sp. PCC 6803 allows formation of a far-red shifted holo-proteorhodopsin in vivo

scientific article published on 23 February 2018

Demonstration and characterization of phosphate transport in mammalian peroxisomes

scientific article published on August 2005

Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae

scientific article published on 2 April 2018

Deuterium isotope effects in the photocycle transitions of the photoactive yellow protein

scientific article published on February 2003

Development of a Light-Dependent Protein Histidine Kinase

scientific article published on 01 January 2020

Differentiation of function among the RsbR paralogs in the general stress response of Bacillus subtilis with regard to light perception

scientific article published on 27 January 2012

Dynamics of cell wall structure in Saccharomyces cerevisiae

scientific article

Dynamics of protein and chromophore structural changes in the photocycle of photoactive yellow protein monitored by time-resolved optical rotatory dispersion

scientific article published in February 2003

Early molecular events in the photoactive yellow protein: role of the chromophore photophysics

scientific article

Effect of hxk2 deletion and HAP4 overexpression on fermentative capacity in Saccharomyces cerevisiae

scientific article published on 20 December 2007

Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis

scientific article

Energetics and role of the hydrophobic interaction during photoreaction of the BLUF domain of AppA.

scientific article

Energy biotechnology with cyanobacteria.

scientific article published on 17 June 2009

Engineering a cyanobacterial cell factory for production of lactic acid

scientific article (publication date: October 2012)

Engineering cyanobacteria for direct biofuel production from CO2.

scientific article

Engineering photocycle dynamics. Crystal structures and kinetics of three photoactive yellow protein hinge-bending mutants

scientific article

Ethylene production with engineered Synechocystis sp PCC 6803 strains

scientific article published on 23 February 2017

Exploiting Day- and Night-Time Metabolism of Synechocystis sp. PCC 6803 for Fitness-Coupled Fumarate Production around the Clock

scientific article published on 01 October 2019

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803.

scientific article published on 26 June 2014

Exploring the low photosynthetic efficiency of cyanobacteria in blue light using a mutant lacking phycobilisomes

scientific article published on 28 February 2019

Expression of holo -proteorhodopsin in Synechocystis sp. PCC 6803

scientific article published on 08 February 2016

First identification of a 2-ketoglutarate/isocitrate transport system in mammalian peroxisomes and its characterization

scientific article published on 20 July 2006

Fluorescence resonance energy transfer (FRET) applications using green fluorescent protein. Energy transfer to the endogenous chromophores of phycobilisome light-harvesting complexes

scientific article published on 01 January 2002

Functional Expression of Gloeobacter Rhodopsin in Synechocystis sp. PCC6803.

scientific article

Functional Expression of Rhodopsin in PSI-Less sp. PCC6803

scientific article published on 29 March 2019

Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

scientific article published on 8 April 2016

Genetic engineering of Synechocystis sp. PCC6803 for poly-β-hydroxybutyrate overproduction

Genome-wide identification of fungal GPI proteins

scientific article published in July 2003

Helix formation is a dynamical bottleneck in the recovery reaction of Photoactive Yellow Protein

scientific article published in July 2008

Hydrogen-bond network probed by time-resolved optoacoustic spectroscopy: photoactive yellow protein and the effect of E46Q and E46A mutations

scientific article published in May 2005

Hypothesis: versatile function of ferredoxin-NADP+ reductase in cyanobacteria provides regulation for transient photosystem I-driven cyclic electron flow

scientific article published on 01 April 2002

Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions

scientific article published in February 2008

Identification and quantitation of newly synthesized proteins in Escherichia coli by enrichment of azidohomoalanine-labeled peptides with diagonal chromatography.

scientific article published on 25 March 2009

In vivo effects on photosynthesis gene expression of base pair exchanges in the gene encoding the light-responsive BLUF domain of AppA in Rhodobacter sphaeroides

scientific article published on 21 May 2010

In vivo mutational analysis of YtvA from Bacillus subtilis: mechanism of light activation of the general stress response

scientific article published on 6 July 2009

Incoherent manipulation of the photoactive yellow protein photocycle with dispersed pump-dump-probe spectroscopy

scientific article

Increased stability of recombinant plasmids by Tn1000 insertion in chemostat cultures of recombinantEscherichia coli GT123

article

Increasing the Photoautotrophic Growth Rate of Synechocystis sp. PCC 6803 by Identifying the Limitations of Its Cultivation.

scientific article published on 25 March 2018

Influence of the crystalline state on photoinduced dynamics of photoactive yellow protein studied by ultraviolet-visible transient absorption spectroscopy

scientific article

Initial characterization of the primary photochemistry of AppA, a blue-light-using flavin adenine dinucleotide-domain containing transcriptional antirepressor protein from Rhodobacter sphaeroides: a key role for reversible intramolecular proton tran

scientific article published in September 2003

Initial events in the photocycle of photoactive yellow protein

scientific article published on 16 March 2004

Initial steps of signal generation in photoactive yellow protein revealed with femtosecond mid-infrared spectroscopy.

scientific article published in September 2003

Investigation of in vivo cross-talk between key two-component systems of Escherichia coli ⬇️

scientific article published on January 1, 2002

Is the photoactive yellow protein a UV-B/blue light photoreceptor? ⬇️

scientific article published on January 25, 2011

Kinase activity of ArcB from Escherichia coli is subject to regulation by both ubiquinone and demethylmenaquinone

scientific article

Lack of negative charge in the E46Q mutant of photoactive yellow protein prevents partial unfolding of the blue-shifted intermediate

scientific article

Light-induced behavioral responses (;phototaxis') in prokaryotes

scientific article

Light-induced flipping of a conserved glutamine sidechain and its orientation in the AppA BLUF domain

scientific article published in November 2006

Light-induced generation of a protonmotive force and Ca2+-transport in membrane vesicles of Streptococcus cremoris fused with bacteriorhodopsin proteoliposomes

Locked chromophore analogs reveal that photoactive yellow protein regulates biofilm formation in the deep sea bacterium Idiomarina loihiensis

scientific article published in December 2009

Mass spectrometric quantification of the adaptations in the wall proteome of Candida albicans in response to ambient pH ⬇️

scientific article published on September 23, 2010

Measuring and modelling dynamical changes in the structure of photoactive yellow protein

article by Jocelyne Vreede et al published January 2004 in Phase Transitions

Membrane matters: The impact of a nanodisc-bilayer or a detergent microenvironment on the properties of two eubacterial rhodopsins

scientific article published on 28 October 2019

Metabolic engineering of cyanobacteria for the synthesis of commodity products.

scientific article published on 21 April 2015

Microbial systems biology: new frontiers open to predictive microbiology

scientific article published on 06 May 2008

Modeling the functioning of YtvA in the general stress response in Bacillus subtilis ⬇️

scientific article published on September 1, 2013

Modulation of spectral properties and pump activity of proteorhodopsins by retinal analogues

scientific article published in April 2015

Molecular physiology of the dynamic regulation of carbon catabolite repression in Escherichia coli

scientific article published on 6 March 2014

Nonhierarchical Flux Regulation Exposes the Fitness Burden Associated with Lactate Production in Synechocystis sp. PCC6803.

scientific article

On the Binding of BODIPY‐GTP by the Photosensory Protein YtvA from the Common Soil Bacterium Bacillus subtilis† ⬇️

scientific article published on May 1, 2011

On the Involvement of Single-Bond Rotation in the Primary Photochemistry of Photoactive Yellow Protein ⬇️

scientific article published on September 7, 2011

On the Use of Metabolic Control Analysis in the Optimization of Cyanobacterial Biosolar Cell Factories ⬇️

scientific article published on March 29, 2013

On the function of the various quinone species in Escherichia coli ⬇️

scientific article published on May 30, 2012

On the in vivo redox state of flavin-containing photosensory receptor proteins

scientific article published on 01 January 2014

On the mechanism of activation of the BLUF domain of AppA.

scientific article published on January 2006

On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein ⬇️

scientific article published on November 24, 2010

On the role of aromatic side chains in the photoactivation of BLUF domains

scientific article published on 2 June 2007

On the signaling mechanism and the absence of photoreversibility in the AppA BLUF domain

scientific article published on 13 March 2008

On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals

scientific article published on 26 March 2019

PAS domains. Common structure and common flexibility

scientific article (publication date: 16 May 2003)

Peroxisomal Fatty Acid Uptake Mechanism in Saccharomyces cerevisiae ⬇️

scientific article published on April 9, 2012

Perturbation of the ground-state electronic structure of FMN by the conserved cysteine in phototropin LOV2 domains

scientific article

Photochemical Reactions of the LOV and LOV-Linker Domains of the Blue Light Sensor Protein YtvA.

scientific article published on 20 May 2016

Photocycle of the flavin-binding photoreceptor AppA, a bacterial transcriptional antirepressor of photosynthesis genes

scientific article

Photoionization and electron radical recombination dynamics in photoactive yellow protein investigated by ultrafast spectroscopy in the visible and near-infrared spectral region.

scientific article published on 15 April 2013

Photoisomerization and photoionization of the photoactive yellow protein chromophore in solution

scientific article published on April 2004

Photonfluxostat: A method for light-limited batch cultivation of cyanobacteria at different, yet constant, growth rates

scientific article published in 2016

Photoreaction Dynamics of a Full-Length Protein YtvA and Intermolecular Interaction with RsbRA

scientific article published on 07 December 2020

Photoreceptor proteins, "star actors of modern times": a review of the functional dynamics in the structure of representative members of six different photoreceptor families

scientific article published on January 2004

Photoreceptors in Chemotrophic Prokaryotes: The Case of Acinetobacter spp. Revisited

scientific article published on 04 July 2015

Photosensing in chemotrophic, non-phototrophic bacteria: let there be light sensing too.

scientific article

Photosynthetic production of glycerol by a recombinant cyanobacterium

scientific article published on 23 December 2014

Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222

scientific article published on January 1, 2002

Physiological actions of preservative agents: prospective of use of modern microbiological techniques in assessing microbial behaviour in food preservation

scientific article

Physiological and transcriptional characterization of Saccharomyces cerevisiae strains with modified expression of catabolic regulators

scientific article published on 24 September 2007

Poly-β-hydroxybutyrate (PHB) production by Synechocystis PCC6803 from CO2: Model development

Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae

scientific article published on 04 May 2013

Predicting the signaling state of photoactive yellow protein

scientific article

Primary photochemistry of the dark- and light-adapted states of the YtvA protein from Bacillus subtilis.

scientific article

Primary reactions of the LOV2 domain of phototropin studied with ultrafast mid-infrared spectroscopy and quantum chemistry

scientific article published on July 2009

Prokaryotic phototaxis

scientific article published on 01 January 2009

Proline 68 enhances photoisomerization yield in photoactive yellow protein

scientific article published on 04 May 2011

Proteomic analysis of Candida albicans cell walls reveals covalently bound carbohydrate-active enzymes and adhesins.

scientific article published on August 2004

Proton transfer events in GFP.

scientific article published on 17 August 2011

Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli

scientific article published on March 2002

Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803

scientific article

Reaction pathways of photoexcited retinal in proteorhodopsin studied by pump-dump-probe spectroscopy

scientific article published in December 2009

Red light activates the sigmaB-mediated general stress response of Bacillus subtilis via the energy branch of the upstream signaling cascade

scientific article published on 30 November 2009

Redshifted and Near-infrared Active Analog Pigments Based upon Archaerhodopsin-3

scientific article published on 08 April 2019

Requirement of ArcA for redox regulation in Escherichia coli under microaerobic but not anaerobic or aerobic conditions

scientific article

Resolving protein structure dynamically

scientific article published on 01 January 2005

Response of the thylakoid proteome of Synechocystis sp. PCC 6803 to photohinibitory intensities of orange-red light

scientific article published on 05 October 2018

Retinal-Based Proton Pumping in the Near Infrared

scientific article published on 17 January 2017

Short hydrogen bonds and negative charge in photoactive yellow protein promote fast isomerization but not high quantum yield.

scientific article published on 29 August 2014

Signal transduction in bacteria: phospho-neural network(s) inEscherichia coli?

article by Klaas J. Hellingwerf et al published July 1995 in FEMS Microbiology Reviews

Simplicity in complexity: the photosynthetic reaction center performs as a simple 0.2 V battery

scientific article published on 01 January 2002

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria.

scientific article published on 25 November 2015

Slr1670 from Synechocystis sp. PCC 6803 Is Required for the Re-assimilation of the Osmolyte Glucosylglycerol

scientific article published on 29 August 2016

Spectrally decomposed dark-to-light transitions in Synechocystis sp. PCC 6803.

scientific article published on 29 March 2018

Spectrally decomposed dark-to-light transitions in a PSI-deficient mutant of Synechocystis sp. PCC 6803.

scientific article

Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution

scientific article

Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 Å resolution

article by Daan M. F. van Aalten et al published 22 March 2002 in Acta Crystallographica Section D: Biological Crystallography

Sustained Circadian Rhythms in Continuous Light in Synechocystis sp. PCC6803 Growing in a Well-Controlled Photobioreactor

scientific article published on June 2015

Synechocystis: Not Just a Plug-Bug for CO2, but a Green E. coli

scientific article

Synthesis of 2,3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria.

scientific article

Tetramer formation kinetics in the signaling state of AppA monitored by time-resolved diffusion

scientific article published on 28 April 2006

The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool

scientific article published on 20 November 2009

The effect of trypsin treatment on the incorporation and energy-transducing properties of bacteriorhodopsin in liposomes

The metabolic response of Saccharomyces cerevisiae to continuous heat stress

scientific article published in January 2002

The molecular basis of sensing and responding to light in microorganisms

scientific article

The redox potential of the plastoquinone pool of the cyanobacterium Synechocystis species strain PCC 6803 is under strict homeostatic control

scientific article published on 2 April 2014

The role of two-component regulation systems in the physiology of the bacterial cell.

scientific article published on January 2006

The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein

scientific article

The solution structure of the AppA BLUF domain: insight into the mechanism of light-induced signaling

scientific article published in January 2006

The two photocycles of photoactive yellow protein from Rhodobacter sphaeroides

scientific article published on 20 December 2002

Thinking and decision making, bacterial style: Bacterial Neural Networks, Obernai, France, 7th-12th June 2002.

scientific article

Time-resolved FT-IR spectroscopy traces signal relay within the blue-light receptor AppA.

scientific article published in August 2007

Time-series analysis of the transcriptome and proteome of Escherichia coli upon glucose repression

scientific article published on 3 June 2015

Towards a systems level understanding of the oxygen response of Escherichia coli

scientific article published on January 2014

TraR auto-inducer enhances protein backbone fluctuations in DNA binding domain

scientific article

Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan

scientific article

Transient exposure of hydrophobic surface in the photoactive yellow protein monitored with Nile Red.

scientific article published on March 2002

Tryptophan fluorescence as a reporter for structural changes in photoactive yellow protein elicited by photo-activation ⬇️

scientific article published on March 1, 2013

Tryptophan fluorescence monitors structural changes accompanying signalling state formation in the photocycle of photoactive yellow protein

scientific article

Ultrafast dynamics of isolated model photoactive yellow protein chromophores: "Chemical perturbation theory" in the laboratory

scientific article published on 01 March 2005

Uncoupling of substrate-level phosphorylation in Escherichia coli during glucose-limited growth ⬇️

scientific article published on July 27, 2012

Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803

scientific article published on 02 July 2020

pH Dependence of the photoactive yellow protein photocycle recovery reaction reveals a new late photocycle intermediate with a deprotonated chromophore

scientific article

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