List of works - Beata Wielgus-Kutrowska

1,N6-ethenoadenine and other Fluorescent Nucleobase Analogues as Substrates for Purine-Nucleoside Phosphorylases: Spectroscopic and Kinetic Studies

scientific article published on 11 October 2017

1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state

scientific article

9-Deazaguanine derivatives connected by a linker to difluoromethylene phosphonic acid are slow-binding picomolar inhibitors of trimeric purine nucleoside phosphorylase

scientific article

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

scientific article published on 21 May 2015

Analytical ultracentrifugation as a tool in the studies of aggregation of the fluorescent marker, Enhanced Green Fluorescent Protein

scientific article published on 01 March 2020

Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.

scientific article

Crystal structure of calf spleen purine nucleoside phosphorylase with two full trimers in the asymmetric unit: important implications for the mechanism of catalysis

scientific article published in September 2004

Enzymatic synthesis of highly fluorescent 8-azapurine ribosides using a purine nucleoside phosphorylase reverse reaction: variable ribosylation sites.

scientific article

Helicobacter pylori purine nucleoside phosphorylase shows new distribution patterns of open and closed active site conformations and unusual biochemical features.

scientific article published on 12 February 2018

Heterodimerizing helices as tools for nanoscale control of the organization of protein-protein and protein-quantum dots

scientific article published on 24 September 2019

Homooligomerization is needed for stability: a molecular modelling and solution study of Escherichia coli purine nucleoside phosphorylase

scientific article published on April 2014

How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles

scientific article published on 23 February 2016

In the quest for new targets for pathogen eradication: the adenylosuccinate synthetase from the bacterium Helicobacter pylori

scientific article published on 01 December 2018

Interactions of trimeric purine nucleoside phosphorylases with ground state analogues--calorimetric and fluorimetric studies

scientific article published in May 2003

Kinetic properties of Cellulomonas sp. purine nucleoside phosphorylase with typical and non-typical substrates: implications for the reaction mechanism

scientific article

Kinetics of binding of multisubstrate analogue inhibitor (2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine) with trimeric purine nucleoside phosphorylase

scientific article published in January 2007

New phosphate binding sites in the crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with phosphate and formycin A

scientific article

Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation

scientific article published on 20 April 2018

On the analysis of fluorimetric titration curves of purine nucleoside phosphorylase

scientific article

Open and closed conformation of the E. coli purine nucleoside phosphorylase active center and implications for the catalytic mechanism

scientific article

Overexpressed proteins may act as mops removing their ligands from the host cells: a case study of calf PNP.

scientific article published on 11 December 2009

Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP).

scientific article

Part-of-the-sites binding and reactivity in the homooligomeric enzymes - facts and artifacts

scientific article published on 2 February 2018

Probing the mechanism of purine nucleoside phosphorylase by steady-state kinetic studies and ligand binding characterization determined by fluorimetric titrations

scientific article published on 31 March 2006

Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme

scientific article

Purine nucleoside phosphorylase from Cellulomonas sp.: physicochemical properties and binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic fluorescence, and by protective effects of ligands on thermal inactivation of

scientific article published on 01 June 2002

Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations.

scientific article

Still a Long Way to Fully Understanding the Molecular Mechanism of Escherichia coli Purine Nucleoside Phosphorylase

scientific article published in 2013

Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors.

scientific article

Tri-Cyclic Nucleobase Analogs and their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives

scientific article published on 16 April 2019

Tricyclic Nucleobase Analogs and Their Ribosides as Substrates and Inhibitors of Purine-Nucleoside Phosphorylases III. Aminopurine Derivatives

scientific article published on 05 February 2020

Tricyclic nitrogen base 1,N6-ethenoadenine and its ribosides as substrates for purine-nucleoside phosphorylases: Spectroscopic and kinetic studies.

scientific article published on 29 January 2018

Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state.

scientific article published on 17 September 2012

Two fluorogenic substrates for purine nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme.

scientific article

Validation of the catalytic mechanism of Escherichia coli purine nucleoside phosphorylase by structural and kinetic studies

scientific article

β2-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide

scientific article published on 28 November 2018

List of works by Beata Wielgus-Kutrowska

1,N6-ethenoadenine and other Fluorescent Nucleobase Analogues as Substrates for Purine-Nucleoside Phosphorylases: Spectroscopic and Kinetic Studies

1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state

9-Deazaguanine derivatives connected by a linker to difluoromethylene phosphonic acid are slow-binding picomolar inhibitors of trimeric purine nucleoside phosphorylase

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

Analytical ultracentrifugation as a tool in the studies of aggregation of the fluorescent marker, Enhanced Green Fluorescent Protein

Cloning, expression, purification, and some properties of calf purine nucleoside phosphorylase.

Crystal structure of calf spleen purine nucleoside phosphorylase with two full trimers in the asymmetric unit: important implications for the mechanism of catalysis

Enzymatic synthesis of highly fluorescent 8-azapurine ribosides using a purine nucleoside phosphorylase reverse reaction: variable ribosylation sites.

Helicobacter pylori purine nucleoside phosphorylase shows new distribution patterns of open and closed active site conformations and unusual biochemical features.

Heterodimerizing helices as tools for nanoscale control of the organization of protein-protein and protein-quantum dots

Homooligomerization is needed for stability: a molecular modelling and solution study of Escherichia coli purine nucleoside phosphorylase

How can macromolecular crowding inhibit biological reactions? The enhanced formation of DNA nanoparticles

In the quest for new targets for pathogen eradication: the adenylosuccinate synthetase from the bacterium Helicobacter pylori

Interactions of trimeric purine nucleoside phosphorylases with ground state analogues--calorimetric and fluorimetric studies

Kinetic properties of Cellulomonas sp. purine nucleoside phosphorylase with typical and non-typical substrates: implications for the reaction mechanism

Kinetics of binding of multisubstrate analogue inhibitor (2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine) with trimeric purine nucleoside phosphorylase

New phosphate binding sites in the crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with phosphate and formycin A

Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation

On the analysis of fluorimetric titration curves of purine nucleoside phosphorylase

Open and closed conformation of the E. coli purine nucleoside phosphorylase active center and implications for the catalytic mechanism

Overexpressed proteins may act as mops removing their ligands from the host cells: a case study of calf PNP.

Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP).

Part-of-the-sites binding and reactivity in the homooligomeric enzymes - facts and artifacts

Probing the mechanism of purine nucleoside phosphorylase by steady-state kinetic studies and ligand binding characterization determined by fluorimetric titrations

Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme

Purine nucleoside phosphorylase from Cellulomonas sp.: physicochemical properties and binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic fluorescence, and by protective effects of ligands on thermal inactivation of

Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations.

Still a Long Way to Fully Understanding the Molecular Mechanism of Escherichia coli Purine Nucleoside Phosphorylase

Thermodynamic studies of interactions of calf spleen PNP with acyclic phosphonate inhibitors.

Tri-Cyclic Nucleobase Analogs and their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives

Tricyclic Nucleobase Analogs and Their Ribosides as Substrates and Inhibitors of Purine-Nucleoside Phosphorylases III. Aminopurine Derivatives

Tricyclic nitrogen base 1,N6-ethenoadenine and its ribosides as substrates for purine-nucleoside phosphorylases: Spectroscopic and kinetic studies.

Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state.

Two fluorogenic substrates for purine nucleoside phosphorylase, selective for mammalian and bacterial forms of the enzyme.

Validation of the catalytic mechanism of Escherichia coli purine nucleoside phosphorylase by structural and kinetic studies

β2-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide