List of works - Alan N Engelman

A New Class of Allosteric HIV-1 Integrase Inhibitors Identified by Crystallographic Fragment Screening of the Catalytic Core Domain

scientific article

A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75

scientific article

A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin

scientific article published on 8 February 2016

A supramolecular assembly mediates lentiviral DNA integration ⬇️

scientific article published on January 2017

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo

scientific article

Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation ⬇️

scientific article published on April 22, 2013

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

scientific article

Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication

scientific article

Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase.

scientific article

Biochemical characterization of novel retroviral integrase proteins

scientific article (publication date: 2013)

Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo

scientific article published on 15 June 2016

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication

scientific article published on December 2004

Correction: Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration

scientific article published on 10 October 2016

Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration

scientific article

Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model

scientific article published on 09 December 2019

Distinct viral reservoirs in individuals with spontaneous control of HIV-1

scientific article published on 26 August 2020

Distribution and Redistribution of HIV-1 Nucleocapsid Protein in Immature, Mature, and Integrase-Inhibited Virions: a Role for Integrase in Maturation ⬇️

scientific article

Efficient Transduction of LEDGF/p75 Mutant Cells by Gain-of-Function HIV-1 Integrase Mutant Viruses.

scientific article

Embryonic Lethality Due to Arrested Cardiac Development in Psip1/Hdgfrp2 Double-Deficient Mice

scientific article

Engineered Murine HSCs Reconstitute Multi-lineage Hematopoiesis and Adaptive Immunity

scientific article published on December 2016

Engineered hyperactive integrase for concerted HIV-1 DNA integration

scientific article

Establishment and Reversal of HIV-1 Latency in Naive and Central Memory CD4+ T Cells In Vitro.

scientific article published on 29 June 2016

Flexible use of nuclear import pathways by HIV-1.

scientific article

Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase

scientific article

HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases

scientific article

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor

scientific article

Haematopoietic stem and progenitor cells from human pluripotent stem cells.

scientific article published on 17 May 2017

Host and viral determinants for MxB restriction of HIV-1 infection

scientific article

Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration

scientific article

Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75

scientific article

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase

scientific article

Identification of host proteins required for HIV infection through a functional genomic screen

scientific article

Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding.

scientific article published on 11 February 2014

Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity

scientific article

Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration.

scientific article

Key determinants of target DNA recognition by retroviral intasomes

scientific article published on 30 April 2015

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration

scientific article

Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration

scientific article

Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication

scientific article published on October 2005

Molecular mechanisms of retroviral integration site selection

scientific article

Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition

scientific article published on 29 August 2019

Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors ⬇️

scientific article published on March 21, 2012

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import

scientific article

Permeability of the HIV-1 capsid to metabolites modulates viral DNA synthesis

scientific article published on 17 December 2020

Proceedings of the Frontiers of Retrovirology Conference 2016.

scientific article published on 26 September 2016

Retroviral DNA Integration

scientific article

Retroviral Integrase Structure and DNA Recombination Mechanism

scientific article

Retroviral intasome assembly and inhibition of DNA strand transfer

scientific article

Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74.

scientific article published on 13 April 2016

Sites of retroviral DNA integration: From basic research to clinical applications

scientific article published on 28 October 2015

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75

scientific article

Structural and mechanistic bases for a potent HIV-1 capsid inhibitor

scientific article

Structural basis for functional tetramerization of lentiviral integrase

scientific article (publication date: July 2009)

Structural basis for nuclear import of splicing factors by human Transportin 3

scientific article

Structural basis for retroviral integration into nucleosomes.

scientific article

Structural basis for spumavirus GAG tethering to chromatin ⬇️

scientific article

Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75.

scientific article

Structural basis of second-generation HIV integrase inhibitor action and viral resistance

scientific article

Structural biology of retroviral DNA integration

scientific article published on 8 January 2011

Structure-based modeling of the functional HIV-1 intasome and its inhibition

scientific article

Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness

scientific article published on 7 September 2006

Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding

scientific article

The A128T Resistance Mutation Reveals Aberrant Protein Multimerization as the Primary Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors ⬇️

scientific article published on April 24, 2013

The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes

scientific article published on 18 March 2016

The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency

scientific article published on 24 February 2016

The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b

scientific article published on 09 March 2020

The SET complex acts as a barrier to autointegration of HIV-1

scientific article

The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes

scientific article

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication

scientific article

The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase

scientific article

The structural biology of HIV-1: mechanistic and therapeutic insights

scientific article published on 16 March 2012

The ups and downs of gene expression and retroviral DNA integration

scientific article

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity

scientific article

Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin

scientific article

rigrag: high-resolution mapping of genic targeting preferences during HIV-1 integration in vitro and in vivo

scientific article published on 01 July 2021

List of works by Alan N Engelman

A New Class of Allosteric HIV-1 Integrase Inhibitors Identified by Crystallographic Fragment Screening of the Catalytic Core Domain

A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75

A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin

A supramolecular assembly mediates lentiviral DNA integration ⬇️

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo

Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation ⬇️

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication

Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase.

Biochemical characterization of novel retroviral integrase proteins

Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication

Correction: Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration

Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration

Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model

Distinct viral reservoirs in individuals with spontaneous control of HIV-1

Distribution and Redistribution of HIV-1 Nucleocapsid Protein in Immature, Mature, and Integrase-Inhibited Virions: a Role for Integrase in Maturation ⬇️

Efficient Transduction of LEDGF/p75 Mutant Cells by Gain-of-Function HIV-1 Integrase Mutant Viruses.

Embryonic Lethality Due to Arrested Cardiac Development in Psip1/Hdgfrp2 Double-Deficient Mice

Engineered Murine HSCs Reconstitute Multi-lineage Hematopoiesis and Adaptive Immunity

Engineered hyperactive integrase for concerted HIV-1 DNA integration

Establishment and Reversal of HIV-1 Latency in Naive and Central Memory CD4+ T Cells In Vitro.

Flexible use of nuclear import pathways by HIV-1.

Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase

HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor

Haematopoietic stem and progenitor cells from human pluripotent stem cells.

Host and viral determinants for MxB restriction of HIV-1 infection

Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration

Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase

Identification of host proteins required for HIV infection through a functional genomic screen

Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding.

Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity

Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration.

Key determinants of target DNA recognition by retroviral intasomes

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration

Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration

Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication

Molecular mechanisms of retroviral integration site selection

Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition

Multimode, Cooperative Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors ⬇️

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import

Permeability of the HIV-1 capsid to metabolites modulates viral DNA synthesis

Proceedings of the Frontiers of Retrovirology Conference 2016.

Retroviral DNA Integration

Retroviral Integrase Structure and DNA Recombination Mechanism

Retroviral intasome assembly and inhibition of DNA strand transfer

Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74.

Sites of retroviral DNA integration: From basic research to clinical applications

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75

Structural and mechanistic bases for a potent HIV-1 capsid inhibitor

Structural basis for functional tetramerization of lentiviral integrase

Structural basis for nuclear import of splicing factors by human Transportin 3

Structural basis for retroviral integration into nucleosomes.

Structural basis for spumavirus GAG tethering to chromatin ⬇️

Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75.

Structural basis of second-generation HIV integrase inhibitor action and viral resistance

Structural biology of retroviral DNA integration

Structure-based modeling of the functional HIV-1 intasome and its inhibition

Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness

Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding

The A128T Resistance Mutation Reveals Aberrant Protein Multimerization as the Primary Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors ⬇️

The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes

The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency

The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b

The SET complex acts as a barrier to autointegration of HIV-1

The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication

The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase

The structural biology of HIV-1: mechanistic and therapeutic insights

The ups and downs of gene expression and retroviral DNA integration

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity

Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin

rigrag: high-resolution mapping of genic targeting preferences during HIV-1 integration in vitro and in vivo