List of works - Niels C Bols

A CXC chemokine sequence isolated from the rainbow trout Oncorhynchus mykiss resembles the closely related interferon-gamma-inducible chemokines CXCL9, CXCL10 and CXCL11

scientific article published on 01 October 2002

A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes

scientific article published on 18 August 2006

A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.

scientific article published on 24 April 2010

Ammonia-containing industrial effluents, lethal to rainbow trout, induce vacuolisation and Neutral Red uptake in the rainbow trout gill cell line, RTgill-W1.

scientific article published on February 2009

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV.

scientific article

Animal cell cultures in microsporidial research: their general roles and their specific use for fish microsporidia.

scientific article published on 30 January 2009

Applying whole water samples to cell bioassays for detecting dioxin-like compounds at contaminated sites.

scientific article

Applying whole-water samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent

scientific article published on September 2002

Atlantic salmon endothelial cells from the heart were more susceptible than fibroblasts from the bulbus arteriosus to four RNA viruses but protected from two viruses by dsRNA pretreatment.

scientific article published on 4 September 2017

CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine

scientific article published on 10 February 2018

Calreticulin in rainbow trout: a limited response to endoplasmic reticulum (ER) stress

scientific article published on 6 April 2007

Carbon nanotube compared with carbon black: effects on bacterial survival against grazing by ciliates and antimicrobial treatments ⬇️

scientific article published on February 7, 2012

Cell-based data to predict the toxicity of chemicals to fish. Commentary on the manuscript by Rodrigues et al., 2019. Cell-based assays seem not to accurately predict fish short-term toxicity of pesticides. Environmental Pollution 252:476-482

scientific article published on 01 November 2019

Characterization of p53 expression in rainbow trout

scientific article

Characterization of rainbow trout CHK2 and its potential as a genotoxicity biomarker

scientific article published on 24 November 2008

Characterization of the continuous skin fibroblastoid cell line, WE-skin11f, from walleye (Sander vitreus)

scientific article published on 11 September 2019

Cloning and characterization of cDNA clones encoding CD9 from Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).

scientific article published on 11 October 2002

Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss.

scientific article published in March 2005

Comparing a ciliate and a fish cell line for their sensitivity to several classes of toxicants by the novel application of multiwell filter plates to Tetrahymena.

scientific article

Cytotoxicity of metals common in mining effluent to rainbow trout cell lines and to the ciliated protozoan, Tetrahymena thermophila.

scientific article published on 21 January 2005

Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells

scientific article published on 16 May 2014

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

scientific article published on 7 August 2015

Developing a list of reference chemicals for testing alternatives to whole fish toxicity tests.

scientific article published on 22 August 2008

Development and characterization of an endothelial cell line from the bulbus arteriosus of walleye, Sander vitreus.

scientific article

Development of a cell line from the American eel brain expressing endothelial cell properties

scientific article published on 29 December 2015

Development of a solvent-free, solid-phase in vitro bioassay using vertebrate cells

scientific article published on May 2006

Development of a walleye spleen stromal cell line sensitive to viral hemorrhagic septicemia virus (VHSV IVb) and to protection by synthetic dsRNA.

scientific article

Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA).

scientific article published on September 2016

Effect of acid blue 80, an anthracenedione dye, on rainbow trout liver, gill and gut cells in vitro ⬇️

scientific article published on August 6, 2011

Effect of selenomethionine on cell viability and heat shock protein 70 levels in rainbow trout intestinal epithelial cells at hypo-, normo-, and hyper-thermic temperatures

scientific article published on 23 July 2018

Effect of suboptimal temperature on the regulation of endogenous antigen presentation in a rainbow trout hypodermal fibroblast cell line

scientific article published on 26 June 2019

Effects of ibuprofen on the viability and proliferation of rainbow trout liver cell lines and potential problems and interactions in effects assessment

scientific article

Effects of viral hemorrhagic septicemia virus (VHSV) on the rainbow trout (Oncorhynchus mykiss) monocyte cell line RTS-11.

scientific article

Evaluating the toxic potential of benzothiazoles with the rainbow trout cell lines, RTgill-W1 and RTL-W1.

scientific article published on 28 April 2016

Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability

scientific article published on March 2004

Evaluation of a porcine lens and fluorescence assay approach for in vitro ocular toxicological investigations

scientific article

Evolution of IFN subgroups in bony fish - 1:Group I-III IFN exist in early ray-finned fish, with group II IFN subgroups present in the Holostean spotted gar, Lepisosteus oculatus

scientific article published on 15 October 2019

Expression of tapasin in rainbow trout tissues and cell lines and up regulation in a monocyte/macrophage cell line (RTS11) by a viral mimic and viral infection

scientific article published on 7 December 2013

Fish Cell Lines as Rapid and Inexpensive Screening and Supplemental Tools for Whole Effluent Testing

Fish cell lines as rapid and inexpensive screening and supplemental tools for whole effluent testing

scientific article published in July 2008

Fish viruses stored in RNAlater can remain infectious and even be temporarily protected from inactivation by heat or by tissue homogenates

scientific article published on 26 December 2017

Genomic cloning of novel isotypes of the rainbow trout interleukin-8.

scientific article published on 15 April 2003

Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss

scientific article

Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss).

scientific article published on May 2004

Impact of carbon nanotubes on the ingestion and digestion of bacteria by ciliated protozoa

scientific article published on 11 May 2008

Improving a fish intestinal barrier model by combining two rainbow trout cell lines: epithelial RTgutGC and fibroblastic RTgutF

scientific article published on 29 June 2019

In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells

scientific article

In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish.

scientific article published on 18 November 2010

Induction of antiviral genes, Mx and vig-1, by dsRNA and Chum salmon reovirus in rainbow trout monocyte/macrophage and fibroblast cell lines

scientific article published on February 2007

Induction of rainbow trout MH class I and accessory proteins by viral haemorrhagic septicaemia virus.

scientific article published on 5 March 2014

Initiation of a zebrafish blastula cell line on rainbow trout stromal cells and subsequent development under feeder-free conditions into a cell line, ZEB2J.

scientific article

Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout

scientific article

Molecular cloning and characterization of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein beta

scientific article published on 28 May 2003

Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout.

scientific article published in May 2010

Productive infection of Piscirickettsia salmonis in macrophages and monocyte-like cells from rainbow trout, a possible survival strategy

scientific article

Proteomic profiles of white sucker (Catostomus commersonii) sampled from within the Thunder Bay Area of Concern reveal up-regulation of proteins associated with tumor formation and exposure to environmental estrogens

scientific article published on 30 January 2012

Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins.

scientific article published on 20 September 2013

Regulation of endogenous antigen presentation in response to suboptimal temperatures in a walleye skin fibroblast cell line

scientific article published on 15 November 2019

Response of the rainbow trout monocyte/macrophage cell line, RTS11 to the water molds Achlya and Saprolegnia

scientific article published on 3 January 2007

Responses of an American eel brain endothelial-like cell line to selenium deprivation and to selenite, selenate, and selenomethionine additions in different exposure media

scientific article

Responses of rainbow trout intestinal epithelial cells to different kinds of nutritional deprivation.

scientific article published on 12 May 2018

Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome

scientific article published on 12 October 2020

Senescence-associated β-galactosidase staining in fish cell lines and primary cultures from several tissues and species, including rainbow trout coelomic fluid and milt

scientific article

Sequencing and expression of the second allele of the interleukin-1beta1 gene in rainbow trout (Oncorhynchus mykiss): identification of a novel SINE in the third intron.

scientific article

Single and combined toxicity of pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line RTL-W1

article

Single and combined toxicity of pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line RTL-W1.

scientific article

Surfactant and UV-B-induced damage of the cultured bovine lens

scientific article

Survival and growth of Tetrahymena thermophila in media that are conventionally used for piscine and mammalian cells

scientific article published on October 2013

Tapasin's protein interactions in the rainbow trout peptide-loading complex.

scientific article published on 15 December 2017

The cloning and inducible expression of the rainbow trout ERp57 gene

scientific article published on 17 November 2012

The functional characterisation of CK-1, a putative CC chemokine from rainbow trout (Oncorhynchus mykiss).

scientific article

The p53 inhibitor, pifithrin-α, disrupts microtubule organization, arrests growth, and induces polyploidy in the rainbow trout gill cell line, RTgill-W1.

scientific article published on 17 August 2015

The p53/HSP70 inhibitor, 2-phenylethynesulfonamide, causes oxidative stress, unfolded protein response and apoptosis in rainbow trout cells.

scientific article published on 2 November 2013

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

scientific article

The use of fish cells in ecotoxicology. The report and recommendations of ECVAM Workshop 47

scientific article published on 01 May 2003

The use of fish-derived cell lines for investigation of environmental contaminants.

scientific article published on January 2003

The use of fish-derived cell lines for investigation of environmental contaminants: an update following OECD's fish toxicity testing framework No. 171.

scientific article published on May 2013

Understanding the pathogenesis of Flavobacterium psychrophilum using the rainbow trout monocyte/macrophage-like cell line, RTS11, as an infection model

scientific article published on 03 December 2019

Use of Tetrahymena thermophila to study the role of protozoa in inactivation of viruses in water

scientific article published on 17 November 2006

Use of the rainbow trout cell lines, RTgill-W1 and RTL-W1 to evaluate the toxic potential of benzotriazoles

scientific article published on 13 November 2015

Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces ⬇️

scientific article published on April 22, 2011

VER-155008 induced Hsp70 proteins expression in fish cell cultures while impeding replication of two RNA viruses

scientific article published on 06 January 2019

VHSV IVb infection and autophagy modulation in the rainbow trout gill epithelial cell line RTgill-W1

scientific article published on 13 August 2020

Xela DS2 and Xela VS2: Two novel skin epithelial-like cell lines from adult African clawed frog (Xenopus laevis) and their response to an extracellular viral dsRNA analogue

scientific article published on 08 June 2020

List of works by Niels C Bols

A CXC chemokine sequence isolated from the rainbow trout Oncorhynchus mykiss resembles the closely related interferon-gamma-inducible chemokines CXCL9, CXCL10 and CXCL11

A cell line (HEW) from embryos of haddock (Melanogrammus aeglefinius) and its capacity to tolerate environmental extremes

A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.

Ammonia-containing industrial effluents, lethal to rainbow trout, induce vacuolisation and Neutral Red uptake in the rainbow trout gill cell line, RTgill-W1.

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV.

Animal cell cultures in microsporidial research: their general roles and their specific use for fish microsporidia.

Applying whole water samples to cell bioassays for detecting dioxin-like compounds at contaminated sites.

Applying whole-water samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent

Atlantic salmon endothelial cells from the heart were more susceptible than fibroblasts from the bulbus arteriosus to four RNA viruses but protected from two viruses by dsRNA pretreatment.

CK-2 of rainbow trout (Oncorhynchus mykiss) has two differentially regulated alleles that encode a functional chemokine

Calreticulin in rainbow trout: a limited response to endoplasmic reticulum (ER) stress

Carbon nanotube compared with carbon black: effects on bacterial survival against grazing by ciliates and antimicrobial treatments ⬇️

Cell-based data to predict the toxicity of chemicals to fish. Commentary on the manuscript by Rodrigues et al., 2019. Cell-based assays seem not to accurately predict fish short-term toxicity of pesticides. Environmental Pollution 252:476-482

Characterization of p53 expression in rainbow trout

Characterization of rainbow trout CHK2 and its potential as a genotoxicity biomarker

Characterization of the continuous skin fibroblastoid cell line, WE-skin11f, from walleye (Sander vitreus)

Cloning and characterization of cDNA clones encoding CD9 from Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).

Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss.

Comparing a ciliate and a fish cell line for their sensitivity to several classes of toxicants by the novel application of multiwell filter plates to Tetrahymena.

Cytotoxicity of metals common in mining effluent to rainbow trout cell lines and to the ciliated protozoan, Tetrahymena thermophila.

Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells

Demonstration of primary cilia and acetylated α-tubulin in fish endothelial, epithelial and fibroblast cell lines

Developing a list of reference chemicals for testing alternatives to whole fish toxicity tests.

Development and characterization of an endothelial cell line from the bulbus arteriosus of walleye, Sander vitreus.

Development of a cell line from the American eel brain expressing endothelial cell properties

Development of a solvent-free, solid-phase in vitro bioassay using vertebrate cells

Development of a walleye spleen stromal cell line sensitive to viral hemorrhagic septicemia virus (VHSV IVb) and to protection by synthetic dsRNA.

Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA).

Effect of acid blue 80, an anthracenedione dye, on rainbow trout liver, gill and gut cells in vitro ⬇️

Effect of selenomethionine on cell viability and heat shock protein 70 levels in rainbow trout intestinal epithelial cells at hypo-, normo-, and hyper-thermic temperatures

Effect of suboptimal temperature on the regulation of endogenous antigen presentation in a rainbow trout hypodermal fibroblast cell line

Effects of ibuprofen on the viability and proliferation of rainbow trout liver cell lines and potential problems and interactions in effects assessment

Effects of viral hemorrhagic septicemia virus (VHSV) on the rainbow trout (Oncorhynchus mykiss) monocyte cell line RTS-11.

Evaluating the toxic potential of benzothiazoles with the rainbow trout cell lines, RTgill-W1 and RTL-W1.

Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability

Evaluation of a porcine lens and fluorescence assay approach for in vitro ocular toxicological investigations

Evolution of IFN subgroups in bony fish - 1:Group I-III IFN exist in early ray-finned fish, with group II IFN subgroups present in the Holostean spotted gar, Lepisosteus oculatus

Expression of tapasin in rainbow trout tissues and cell lines and up regulation in a monocyte/macrophage cell line (RTS11) by a viral mimic and viral infection

Fish Cell Lines as Rapid and Inexpensive Screening and Supplemental Tools for Whole Effluent Testing

Fish cell lines as rapid and inexpensive screening and supplemental tools for whole effluent testing

Fish viruses stored in RNAlater can remain infectious and even be temporarily protected from inactivation by heat or by tissue homogenates

Genomic cloning of novel isotypes of the rainbow trout interleukin-8.

Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss

Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss).

Impact of carbon nanotubes on the ingestion and digestion of bacteria by ciliated protozoa

Improving a fish intestinal barrier model by combining two rainbow trout cell lines: epithelial RTgutGC and fibroblastic RTgutF

In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells

In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish.

Induction of antiviral genes, Mx and vig-1, by dsRNA and Chum salmon reovirus in rainbow trout monocyte/macrophage and fibroblast cell lines

Induction of rainbow trout MH class I and accessory proteins by viral haemorrhagic septicaemia virus.

Initiation of a zebrafish blastula cell line on rainbow trout stromal cells and subsequent development under feeder-free conditions into a cell line, ZEB2J.

Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout

Molecular cloning and characterization of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein beta

Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout.

Productive infection of Piscirickettsia salmonis in macrophages and monocyte-like cells from rainbow trout, a possible survival strategy

Proteomic profiles of white sucker (Catostomus commersonii) sampled from within the Thunder Bay Area of Concern reveal up-regulation of proteins associated with tumor formation and exposure to environmental estrogens

Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins.

Regulation of endogenous antigen presentation in response to suboptimal temperatures in a walleye skin fibroblast cell line

Response of the rainbow trout monocyte/macrophage cell line, RTS11 to the water molds Achlya and Saprolegnia

Responses of an American eel brain endothelial-like cell line to selenium deprivation and to selenite, selenate, and selenomethionine additions in different exposure media

Responses of rainbow trout intestinal epithelial cells to different kinds of nutritional deprivation.

Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome

Senescence-associated β-galactosidase staining in fish cell lines and primary cultures from several tissues and species, including rainbow trout coelomic fluid and milt

Sequencing and expression of the second allele of the interleukin-1beta1 gene in rainbow trout (Oncorhynchus mykiss): identification of a novel SINE in the third intron.

Single and combined toxicity of pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line RTL-W1

Single and combined toxicity of pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line RTL-W1.

Surfactant and UV-B-induced damage of the cultured bovine lens

Survival and growth of Tetrahymena thermophila in media that are conventionally used for piscine and mammalian cells

Tapasin's protein interactions in the rainbow trout peptide-loading complex.

The cloning and inducible expression of the rainbow trout ERp57 gene

The functional characterisation of CK-1, a putative CC chemokine from rainbow trout (Oncorhynchus mykiss).

The p53 inhibitor, pifithrin-α, disrupts microtubule organization, arrests growth, and induces polyploidy in the rainbow trout gill cell line, RTgill-W1.

The p53/HSP70 inhibitor, 2-phenylethynesulfonamide, causes oxidative stress, unfolded protein response and apoptosis in rainbow trout cells.

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

The use of fish cells in ecotoxicology. The report and recommendations of ECVAM Workshop 47

The use of fish-derived cell lines for investigation of environmental contaminants.

The use of fish-derived cell lines for investigation of environmental contaminants: an update following OECD's fish toxicity testing framework No. 171.

Understanding the pathogenesis of Flavobacterium psychrophilum using the rainbow trout monocyte/macrophage-like cell line, RTS11, as an infection model

Use of Tetrahymena thermophila to study the role of protozoa in inactivation of viruses in water