List of works - Carol A. Fierke

A Novel Y-Shaped, S-O-N-O-S-Bridged Cross-Link between Three Residues C22, C44, and K61 Is Frequently Observed in the SARS-CoV-2 Main Protease

A bacterial selection for the directed evolution of pyruvate aldolases.

scientific article published in August 2004

A divalent cation stabilizes the active conformation of the B. subtilis RNase P x pre-tRNA complex: a role for an inner-sphere metal ion in RNase P.

scientific article

A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P

scientific article

Activation and inhibition of histone deacetylase 8 by monovalent cations

scientific article

Active site metal ion in UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches between Fe(II) and Zn(II) depending on cellular conditions

scientific article

Biochemical characterization of the Yersinia YopT protease: cleavage site and recognition elements in Rho GTPases

scientific article

Carbonic Anhydrase: Evolution of the Zinc Binding Site by Nature and by Design

article

Carbonic anhydrase II-based metal ion sensing: Advances and new perspectives

scientific article

Catalytic activity and inhibition of human histone deacetylase 8 is dependent on the identity of the active site metal ion.

scientific article

Characterization and crystal structure of Escherichia coli KDPGal aldolase

scientific article

Characterization of conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme assembly and tRNA processing

scientific article

Combinatorial modulation of protein prenylation

scientific article

Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli

scientific article

Crystal structure of LpxC, a zinc-dependent deacetylase essential for endotoxin biosynthesis

scientific article

Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate

scientific article

Discovering RNA-Protein Interactome by Using Chemical Context Profiling of the RNA-Protein Interface ⬇️

scientific article (publication date: 30 May 2013)

Dissecting allosteric effects of activator-coactivator complexes using a covalent small molecule ligand

scientific article

Eukaryotic ribonuclease P: a plurality of ribonucleoprotein enzymes

scientific article (publication date: 2002)

Evaluation of protein farnesyltransferase substrate specificity using synthetic peptide libraries

scientific article

Evolution reversed: the ability to bind iron restored to the N-lobe of the murine inhibitor of carbonic anhydrase by strategic mutagenesis

scientific journal article

Expanded Dynamic Range of Free Zinc Ion Determination by Fluorescence Anisotropy ⬇️

scientific article published on 01 May 1998

Expansion of protein farnesyltransferase specificity using "tunable" active site interactions: development of bioengineered prenylation pathways.

scientific article

Farnesyl diphosphate analogues with aryl moieties are efficient alternate substrates for protein farnesyltransferase

scientific article

Function and mechanism of zinc metalloenzymes.

scientific article

Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP

scientific article

HDAC8 substrate selectivity is determined by long- and short-range interactions leading to enhanced reactivity for full-length histone substrates compared with peptides.

scientific article published on 6 November 2017

HDAC8 substrates: Histones and beyond

scientific article

Identification of a novel class of farnesylation targets by structure-based modeling of binding specificity

scientific article

Identification of novel peptide substrates for protein farnesyltransferase reveals two substrate classes with distinct sequence selectivities

scientific article

Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates

scientific article

In Search of Aggregation Pathways of IAPP and Other Amyloidogenic Proteins: Finding Answers through NMR Spectroscopy.

scientific article published on 15 May 2014

Influence of a curcumin derivative on hIAPP aggregation in the absence and presence of lipid membranes

scientific article published on 20 November 2015

Inhibition of IAPP Aggregation and Toxicity by Natural Products and Derivatives

scientific article

Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay

scientific article

Kinetics and thermodynamics of metal-binding to histone deacetylase 8.

scientific article published on 13 January 2015

Ligand concentration regulates the pathways of coupled protein folding and binding.

scientific article

Long wavelength fluorescence ratiometric zinc biosensor

scientific article

Mechanism of the Class I KDPG aldolase

scientific article

Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing

scientific article

Molecular recognition of pre-tRNA by Arabidopsis protein-only Ribonuclease P.

scientific article published on 5 September 2017

NMR and XAS reveal an inner-sphere metal binding site in the P4 helix of the metallo-ribozyme ribonuclease P.

scientific article

Phage-Assisted, Active Site-Directed Ligand Evolution with a Genetically Encoded N ε -Butyryl-L-Lysine to Identify a Cellularly Potent and Selective Inhibitor for the ENL YEATS Domain as an Anti-Leukemia Agent

Phage-assisted, active site-directed ligand evolution of a potent and selective histone deacetylase 8 inhibitor

Phosphorylation of Histone Deacetylase 8: Structural and Mechanistic Analysis of the Phosphomimetic S39E Mutant

scientific article published on 04 November 2019

Pre-tRNA turnover catalyzed by the yeast nuclear RNase P holoenzyme is limited by product release

scientific article

Protein-precursor tRNA contact leads to sequence-specific recognition of 5' leaders by bacterial ribonuclease P

scientific article

Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response

scientific article published in 2022

Residue ionization in LpxC directly observed by 67Zn NMR spectroscopy

scientific article

Ribonuclease P Protein Structure: Evolutionary Origins in the Translational Apparatus ⬇️

scientific article published on May 1, 1998

Roles of protein subunits in RNA-protein complexes: lessons from ribonuclease P.

scientific article

Self-assembly of a nine-residue amyloid-forming peptide fragment of SARS corona virus E-protein: mechanism of self aggregation and amyloid-inhibition of hIAPP.

scientific article published on 24 March 2015

SmgGDS-607 Regulation of RhoA GTPase Prenylation Is Nucleotide-Dependent

scientific article published on 10 July 2018

Specific phosphorothioate substitutions probe the active site of Bacillus subtilis ribonuclease P.

scientific article

Structural Studies of Human Histone Deacetylase 8 and Its Site-Specific Variants Complexed with Substrate and Inhibitors † , ‡

scientific article

Structure of the Metal-Dependent Deacetylase LpxC from Yersinia enterocolitica Complexed with the Potent Inhibitor CHIR-090,

scientific article

Structure-based prediction of HDAC6 substrates validated by enzymatic assay reveals determinants of promiscuity and detects new potential substrates

Structures of Metal-Substituted Human Histone Deacetylase 8 Provide Mechanistic Inferences on Biological Function,

scientific article

Synthesis and screening of a CaaL peptide library versus FTase reveals a surprising number of substrates

scientific article

The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions

scientific article

UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase functions through a general acid-base catalyst pair mechanism.

scientific article

Unexpected specificity within dynamic transcriptional protein-protein complexes

scientific article published on 19 October 2020

Zinc hydrolases: the mechanisms of zinc-dependent deacetylases

scientific article

List of works by Carol A. Fierke

A Novel Y-Shaped, S-O-N-O-S-Bridged Cross-Link between Three Residues C22, C44, and K61 Is Frequently Observed in the SARS-CoV-2 Main Protease

A bacterial selection for the directed evolution of pyruvate aldolases.

A divalent cation stabilizes the active conformation of the B. subtilis RNase P x pre-tRNA complex: a role for an inner-sphere metal ion in RNase P.

A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P

Activation and inhibition of histone deacetylase 8 by monovalent cations

Active site metal ion in UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) switches between Fe(II) and Zn(II) depending on cellular conditions

Biochemical characterization of the Yersinia YopT protease: cleavage site and recognition elements in Rho GTPases

Carbonic Anhydrase: Evolution of the Zinc Binding Site by Nature and by Design

Carbonic anhydrase II-based metal ion sensing: Advances and new perspectives

Catalytic activity and inhibition of human histone deacetylase 8 is dependent on the identity of the active site metal ion.

Characterization and crystal structure of Escherichia coli KDPGal aldolase

Characterization of conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme assembly and tRNA processing

Combinatorial modulation of protein prenylation

Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli

Crystal structure of LpxC, a zinc-dependent deacetylase essential for endotoxin biosynthesis

Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate

Discovering RNA-Protein Interactome by Using Chemical Context Profiling of the RNA-Protein Interface ⬇️

Dissecting allosteric effects of activator-coactivator complexes using a covalent small molecule ligand

Eukaryotic ribonuclease P: a plurality of ribonucleoprotein enzymes

Evaluation of protein farnesyltransferase substrate specificity using synthetic peptide libraries

Evolution reversed: the ability to bind iron restored to the N-lobe of the murine inhibitor of carbonic anhydrase by strategic mutagenesis

Expanded Dynamic Range of Free Zinc Ion Determination by Fluorescence Anisotropy ⬇️

Expansion of protein farnesyltransferase specificity using "tunable" active site interactions: development of bioengineered prenylation pathways.

Farnesyl diphosphate analogues with aryl moieties are efficient alternate substrates for protein farnesyltransferase

Function and mechanism of zinc metalloenzymes.

Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP

HDAC8 substrate selectivity is determined by long- and short-range interactions leading to enhanced reactivity for full-length histone substrates compared with peptides.

HDAC8 substrates: Histones and beyond

Identification of a novel class of farnesylation targets by structure-based modeling of binding specificity

Identification of novel peptide substrates for protein farnesyltransferase reveals two substrate classes with distinct sequence selectivities

Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates

In Search of Aggregation Pathways of IAPP and Other Amyloidogenic Proteins: Finding Answers through NMR Spectroscopy.

Influence of a curcumin derivative on hIAPP aggregation in the absence and presence of lipid membranes

Inhibition of IAPP Aggregation and Toxicity by Natural Products and Derivatives

Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay

Kinetics and thermodynamics of metal-binding to histone deacetylase 8.

Ligand concentration regulates the pathways of coupled protein folding and binding.

Long wavelength fluorescence ratiometric zinc biosensor

Mechanism of the Class I KDPG aldolase

Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing

Molecular recognition of pre-tRNA by Arabidopsis protein-only Ribonuclease P.

NMR and XAS reveal an inner-sphere metal binding site in the P4 helix of the metallo-ribozyme ribonuclease P.

Phage-Assisted, Active Site-Directed Ligand Evolution with a Genetically Encoded N ε -Butyryl-L-Lysine to Identify a Cellularly Potent and Selective Inhibitor for the ENL YEATS Domain as an Anti-Leukemia Agent

Phage-assisted, active site-directed ligand evolution of a potent and selective histone deacetylase 8 inhibitor

Phosphorylation of Histone Deacetylase 8: Structural and Mechanistic Analysis of the Phosphomimetic S39E Mutant

Pre-tRNA turnover catalyzed by the yeast nuclear RNase P holoenzyme is limited by product release

Protein-precursor tRNA contact leads to sequence-specific recognition of 5' leaders by bacterial ribonuclease P

Ratiometric Zinc Biosensor Based on Bioluminescence Resonance Energy Transfer: Trace Metal Ion Determination with Tunable Response

Residue ionization in LpxC directly observed by 67Zn NMR spectroscopy

Ribonuclease P Protein Structure: Evolutionary Origins in the Translational Apparatus ⬇️

Roles of protein subunits in RNA-protein complexes: lessons from ribonuclease P.

Self-assembly of a nine-residue amyloid-forming peptide fragment of SARS corona virus E-protein: mechanism of self aggregation and amyloid-inhibition of hIAPP.

SmgGDS-607 Regulation of RhoA GTPase Prenylation Is Nucleotide-Dependent

Specific phosphorothioate substitutions probe the active site of Bacillus subtilis ribonuclease P.

Structural Studies of Human Histone Deacetylase 8 and Its Site-Specific Variants Complexed with Substrate and Inhibitors † , ‡

Structure of the Metal-Dependent Deacetylase LpxC from Yersinia enterocolitica Complexed with the Potent Inhibitor CHIR-090,

Structure-based prediction of HDAC6 substrates validated by enzymatic assay reveals determinants of promiscuity and detects new potential substrates

Structures of Metal-Substituted Human Histone Deacetylase 8 Provide Mechanistic Inferences on Biological Function,

Synthesis and screening of a CaaL peptide library versus FTase reveals a surprising number of substrates

The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions

UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase functions through a general acid-base catalyst pair mechanism.

Unexpected specificity within dynamic transcriptional protein-protein complexes

Zinc hydrolases: the mechanisms of zinc-dependent deacetylases