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List of works by Gyun Min Lee

A DIGE approach for the assessment of differential expression of the CHO proteome under sodium butyrate addition: Effect of Bcl-x(L) overexpression

scientific article published in February 2010

A proteomic approach for identifying cellular proteins interacting with erythropoietin in recombinant Chinese hamster ovary cells

scientific article

A simple analysis system for the estimation of recombination efficiency using fluorescence-activated cell sorting

scientific article published on 31 July 2006

Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment

scientific article published on 9 May 2016

Adaptation of Chinese hamster ovary cells to low culture temperature: cell growth and recombinant protein production

scientific article published on 25 October 2005

Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system

scientific article published on 16 November 2020

Analysis of Golgi pH in Chinese hamster ovary cells using ratiometric pH-sensitive fluorescent proteins

scientific article published on 22 January 2019

Angiopoietin-2 exocytosis is stimulated by sphingosine-1-phosphate in human blood and lymphatic endothelial cells

scientific article

Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells

scientific article

Anti-cell death engineering of CHO cells: co-overexpression of Bcl-2 for apoptosis inhibition, Beclin-1 for autophagy induction

scientific article published on 16 March 2013

Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

scientific article published in January 2006

Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells

scientific article published on May 2008

Autophagy and apoptosis in Chinese hamster ovary cell culture

scientific article published on 20 September 2007

Autophagy and apoptosis of recombinant Chinese hamster ovary cells during fed‐batch culture: Effect of nutrient supplementation ⬇️

scientific article published on May 7, 2011

Autophagy and its implication in Chinese hamster ovary cell culture ⬇️

scientific article published on July 24, 2013

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

scientific article published on 12 November 2019

Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity

scientific article

Bcl-xL overexpression delays the onset of autophagy and apoptosis in hyperosmotic recombinant Chinese hamster ovary cell cultures ⬇️

scientific article published on August 3, 2011

Bcl-xL overexpression does not enhance specific erythropoietin productivity of recombinant CHO cells grown at 33 degrees C and 37 degrees C.

scientific article published in January 2009

BiP Inducer X: An ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture

scientific article published on 05 July 2019

Biological characterization of angiopoietin-3 and angiopoietin-4

scientific article

Biphasic culture strategy based on hyperosmotic pressure for improved humanized antibody production in Chinese hamster ovary cell culture

scientific article published in June 2002

CHO cells in biotechnology for production of recombinant proteins: current state and further potential ⬇️

scientific article published on 09 December 2011

COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity

scholarly article

CRISPR/Cas9-Multiplexed Editing of Chinese Hamster Ovary B4Gal-T1, 2, 3, and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins

article

Calnexin overexpression sensitizes recombinant CHO cells to apoptosis induced by sodium butyrate treatment

scientific article published on 29 July 2008

Case study on human α1-antitrypsin: Recombinant protein titers obtained by commercial ELISA kits are inaccurate

scientific article

Characterization and efficacy of PKH26 as a probe to study the replication history of the human hematopoietic KG1a progenitor cell line

scientific article

Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7.

scientific article published on 19 November 2014

Chemical inhibition of autophagy: Examining its potential to increase the specific productivity of recombinant CHO cell lines

scientific article published on 23 February 2016

Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells

scientific article published on 13 April 2018

Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells ⬇️

scientific article published on July 27, 2011

Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells

scientific article published on 29 May 2020

Comprehensive Characterization of Dihydrofolate Reductase-Mediated Gene Amplification for the Establishment of Recombinant Human Embryonic Kidney 293 Cells Producing Monoclonal Antibodies

scientific article published on 13 December 2020

Comprehensive Physicochemical and Biological Characterization of the Proposed Biosimilar Darbepoetin Alfa, LBDE, and Its Originator Darbepoetin Alfa, NESP®.

scientific article

Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies.

scientific article

Controlling medium osmolality improves the expansion of human articular chondrocytes in serum-free media

scientific article published on 01 October 2010

Correlation between enhancing effect of sodium butyrate on specific productivity and mRNA transcription level in recombinant Chinese hamster ovary cells producing antibody.

scientific article published in June 2007

Current state and perspectives on erythropoietin production ⬇️

scientific article published on July 22, 2012

Designed angiopoietin-1 variant, COMP-Ang1, protects against radiation-induced endothelial cell apoptosis

scholarly article

Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

scientific article

Development of apoptosis‐resistant dihydrofolate reductase‐deficient Chinese hamster ovary cell line ⬇️

scientific article published on June 30, 2003

Development of serum-free medium supplemented with hydrolysates for the production of therapeutic antibodies in CHO cell cultures using design of experiments

scientific article published on 6 March 2009

Differences in optimal pH and temperature for cell growth and antibody production between two Chinese hamster ovary clones derived from the same parental clone.

scientific article published in May 2007

Differential expression of microRNAs in recombinant Chinese hamster ovary cells treated with sodium butyrate using digital RNA counting

article

Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment ⬇️

scientific article published on June 20, 2012

Differential in‐gel electrophoresis (DIGE) analysis of CHO cells under hyperosmotic pressure: Osmoprotective effect of glycine betaine addition ⬇️

scientific article published on January 23, 2012

Differential protein expression in human articular chondrocytes expanded in serum-free media of different medium osmolalities by DIGE.

scientific article

Digital mRNA profiling of N-glycosylation gene expression in recombinant Chinese hamster ovary cells treated with sodium butyrate

scientific article published on 12 December 2013

Double Anti-angiogenic and Anti-inflammatory Protein Valpha Targeting VEGF-A and TNF-α in Retinopathy and Psoriasis ⬇️

scientific article published on February 23, 2011

Double antiangiogenic protein, DAAP, targeting VEGF-A and angiopoietins in tumor angiogenesis, metastasis, and vascular leakage

scientific article

Double-antiangiogenic protein DAAP targeting vascular endothelial growth factor A and angiopoietins attenuates collagen-induced arthritis ⬇️

scientific article published on August 14, 2013

Down-regulation of cold-inducible RNA-binding protein does not improve hypothermic growth of Chinese hamster ovary cells producing erythropoietin.

scientific article published on 15 December 2006

Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietin

scientific article published on 4 November 2006

Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells

scientific article published on 14 October 2008

Effect of Bcl-x(L) overexpression on lactate metabolism in chinese hamster ovary cells producing antibody

scientific article published on 30 September 2013

Effect of Bcl-xL overexpression on apoptosis and autophagy in recombinant Chinese hamster ovary cells under nutrient-deprived condition

scientific article published on July 2009

Effect of Bcl-xL overexpression on sialylation of Fc-fusion protein in recombinant Chinese hamster ovary cell cultures

scientific article published on 28 May 2015

Effect of Ca2+ and Mg2+ concentration in culture medium on the activation of recombinant factor IX produced in Chinese hamster ovary cells.

scientific article

Effect of XIAP overexpression on sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells producing erythropoietin

scientific article published on 30 September 2009

Effect of chemical chaperone addition on production and aggregation of recombinant flag‐tagged COMP‐angiopoietin 1 in chinese hamster ovary cells ⬇️

scientific article published on March 18, 2011

Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells ⬇️

scientific article published on March 25, 2011

Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0 degrees C.

scientific article published in February 2005

Effect of culture pH on recombinant antibody production by a new human cell line, F2N78, grown in suspension at 33.0 °C and 37.0 °C ⬇️

scientific article published on April 4, 2013

Effect of doxycycline-regulated ERp57 expression on specific thrombopoietin productivity of recombinant CHO cells

scientific article published on January 2003

Effect of doxycycline-regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant Chinese hamster ovary cells

scientific article published on March 2004

Effect of doxycycline-regulated protein disulfide isomerase expression on the specific productivity of recombinant CHO cells: thrombopoietin and antibody

scientific article

Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture

scientific article published on 3 January 2014

Effect of glutamine substitution by TCA cycle intermediates on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture

scientific article published on 8 April 2014

Effect of inducible co-overexpression of protein disulfide isomerase and endoplasmic reticulum oxidoreductase on the specific antibody productivity of recombinant Chinese hamster ovary cells

scientific article

Effect of lithium chloride on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture

scientific article published on 19 August 2014

Effect of low culture temperature on specific productivity and transcription level of anti-4-1BB antibody in recombinant Chinese hamster ovary cells

scientific article published in July 2003

Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells

scientific article published in May 2003

Effect of simultaneous application of stressful culture conditions on specific productivity and heterogeneity of erythropoietin in Chinese hamster ovary cells

scientific article published in July 2004

Effect of sodium butyrate on autophagy and apoptosis in Chinese hamster ovary cells

scientific article published on 4 January 2012

Effect of sodium butyrate on the assembly, charge variants, and galactosylation of antibody produced in recombinant Chinese hamster ovary cells

scientific article published on 21 February 2014

Effect of sodium butyrate on the production, heterogeneity and biological activity of human thrombopoietin by recombinant Chinese hamster ovary cells

scientific article

Effect of trisodium citrate treatment on hybridoma cell viability

Effects of culture temperature and pH on flag-tagged COMP angiopoietin-1 (FCA1) production from recombinant CHO cells: FCA1 aggregation ⬇️

scientific article published on April 21, 2011

Efficient Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells by Modulating Intracellular Signaling Pathways in a Feeder/Serum-Free System ⬇️

scientific article published on September 27, 2011

Endoplasmic reticulum-directed recombinant mRNA displays subcellular localization equal to endogenous mRNA during transient expression in CHO cells

scientific article published on 14 September 2016

Enhanced human thrombopoietin production by sodium butyrate addition to serum-free suspension culture of bcl-2-overexpressing CHO cells

scientific article published in January 2005

Enhanced interferon-beta production by CHO cells through elevated osmolality and reduced culture temperature

scientific article published in September 2009

Enhancing effect of low culture temperature on specific antibody productivity of recombinant Chinese hamster ovary cells: clonal variation

scientific article published in November 2004

Expression and purification of recombinant human angiopoietin-1 produced in Chinese hamster ovary cells

scientific article published on May 2007

Expression and purification of recombinant human angiopoietin-2 produced in Chinese hamster ovary cells.

scientific article published on February 2005

Flow cytometric application of helper adenovirus (HAd) containing GFP gene flanked by two parallel loxP sites to evaluation of 293 cre-complementing cell line and monitoring of HAd in Gutless Ad production

scientific article published on May 2004

Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44).

scientific article

Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion

scientific article published on 02 January 2020

Glutamine synthetase gene knockout-human embryonic kidney 293E cells for stable production of monoclonal antibodies.

scientific article published on 23 January 2018

Glyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles

scientific article published on 01 December 2018

Growth factor withdrawal in combination with sodium butyrate addition extends culture longevity and enhances antibody production in CHO cells ⬇️

scientific article published on June 23, 2011

Guidelines for the use and interpretation of assays for monitoring autophagy

scientific article

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

scientific article

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

article by Daniel J Klionsky et al published January 2021 in Autophagy

High-level expression and purification of a designed angiopoietin-1 chimeric protein, COMP-Ang1, produced in Chinese hamster ovary cells

scientific article published in August 2008

Hyperosmotic stress induces autophagy and apoptosis in recombinant Chinese hamster ovary cell culture

scientific article published in April 2010

Improving recombinant bone morphogenetic protein-4 (BMP-4) production by autoregulatory feedback loop removal using BMP receptor-knockout CHO cell lines

scientific article published on 14 November 2018

Improving the production of recombinant human bone morphogenetic protein-4 in Chinese hamster ovary cell cultures by inhibition of undesirable endocytosis

scientific article published on 13 August 2018

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions.

scientific article published on 05 December 2016

Influence of co-down-regulation of caspase-3 and caspase-7 by siRNAs on sodium butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin

scientific article published on 25 August 2007

Influence of culture passages on growth kinetics and adenovirus vector production for gene therapy in monolayer and suspension cultures of HEK 293 cells

scientific article published on 27 May 2004

Influence of down-regulation of caspase-3 by siRNAs on sodium-butyrate-induced apoptotic cell death of Chinese hamster ovary cells producing thrombopoietin

scientific article published on 19 September 2005

Inhibition of sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase-3.

scientific article published in April 2002

Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin

scientific article

Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures

scientific article published on 27 November 2019

Limitations to the comparative proteomic analysis of thrombopoietin producing Chinese hamster ovary cells treated with sodium butyrate

scientific article

Limitations to the development of humanized antibody producing Chinese hamster ovary cells using glutamine synthetase-mediated gene amplification

scientific article published in May 2006

Limitations to the development of recombinant human embryonic kidney 293E cells using glutamine synthetase-mediated gene amplification: Methionine sulfoximine resistance

scientific article published on 8 June 2016

Long-term and sustained COMP-Ang1 induces long-lasting vascular enlargement and enhanced blood flow

scientific article published on 16 June 2005

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

scientific article published on 16 August 2018

Mitigating Clonal Variation in Recombinant Mammalian Cell Lines

scientific article published on 18 March 2019

Monitoring of autophagy in Chinese hamster ovary cells using flow cytometry ⬇️

scientific article published on November 28, 2011

Multi-copy targeted integration for accelerated development of high-producing CHO cells

scientific article published on 24 August 2020

Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2

scientific article

Multiplex secretome engineering enhances recombinant protein production and purity

scientific article published on 20 April 2020

Novel glycosylated VEGF decoy receptor fusion protein, VEGF-Grab, efficiently suppresses tumor angiogenesis and progression.

scientific article published on 22 December 2014

Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture

scientific article published in February 2008

Oligomerization and multimerization are critical for angiopoietin-1 to bind and phosphorylate Tie2.

scientific article

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

scientific article published on 10 April 2015

Overexpression of PACEsol improves BMP-7 processing in recombinant CHO cells ⬇️

scientific article published on December 15, 2012

Protein reference mapping of dihydrofolate reductase-deficient CHO DG44 cell lines using 2-dimensional electrophoresis

scientific article

Proteome analysis of antibody-expressing CHO cells in response to hyperosmotic pressure

scientific article

Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells.

scientific article published on 10 March 2017

Proteomic analysis of host cell protein dynamics in the supernatant of Fc-fusion protein-producing CHO DG44 and DUKX-B11 cell lines in batch and fed-batch cultures.

scientific article published on 19 June 2017

Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates

scientific article published on February 2011

Rapamycin treatment inhibits CHO cell death in a serum‐free suspension culture by autophagy induction ⬇️

scientific article published on June 7, 2012

Rapid selection of single cells with high antibody production rates by microwell array ⬇️

scientific article published on September 8, 2011

Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference

scientific article published on 02 April 2019

Reduction of ammonia and lactate through the coupling of glutamine synthetase selection and downregulation of lactate dehydrogenase-A in CHO cells

scientific article published on 04 October 2016

Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression

scientific article

Revealing key determinants of clonal variation in transgene expression in recombinant CHO cells using targeted genome editing

scientific article published on 14 November 2018

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

scientific article published on 16 January 2017

Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification

scientific article

Simple and Robust N-Glycan Analysis Based on Improved 2-Aminobenzoic Acid Labeling for Recombinant Therapeutic Glycoproteins

scientific article published on 21 March 2018

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering

scientific article published on 12 March 2019

Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture

scientific article published on 8 February 2016

The enhancement of recombinant protein production by polymer nanospheres in cell suspension culture

scientific article published in May 2005

The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures

scientific article published on 25 April 2016

Understanding of altered N-glycosylation-related gene expression in recombinant Chinese hamster ovary cells subjected to elevated ammonium concentration by digital mRNA counting

scientific article published on 10 March 2015

Understanding of decreased sialylation of Fc-fusion protein in hyperosmotic recombinant Chinese hamster ovary cell culture: N-glycosylation gene expression and N-linked glycan antennary profile.

scientific article published on 7 March 2017

Untangling the mechanism of 3-methyladenine in enhancing the specific productivity: Transcriptome analysis of recombinant Chinese hamster ovary cells treated with 3-methyladenine

scientific article published on 12 July 2018

Use of Flp-mediated cassette exchange in the development of a CHO cell line stably producing erythropoietin

scientific article

Use of NaCl prevents aggregation of recombinant COMP-angiopoietin-1 in Chinese hamster ovary cells.

scientific article published on 24 June 2009

Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept

scientific article published on 23 January 2018

Valeric acid induces cell cycle arrest at G1 phase in CHO cell cultures and improves recombinant antibody productivity

scientific article published on 11 December 2015

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

scientific article

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