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List of works by Gregory Stephanopoulos

13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica

scientific article published on 4 July 2016

13C isotope-assisted methods for quantifying glutamine metabolism in cancer cells

scientific article

A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors

scientific article

A roadmap for interpreting (13)C metabolite labeling patterns from cells

scientific article

A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates

scientific article published on 10 October 2007

Accessing Nature's diversity through metabolic engineering and synthetic biology

scientific article

Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis

scientific article published on 7 September 2007

Akt regulation of glycolysis mediates bioenergetic stability in epithelial cells

scientific article published on 14 December 2017

An elementary metabolite unit (EMU) based method of isotopically nonstationary flux analysis

scientific article published in February 2008

Analysis of heterologous taxadiene production in K- and B-derived Escherichia coli ⬇️

scientific article published on August 18, 2011

Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations

scientific article published on 30 October 2009

Application of metabolic controls for the maximization of lipid production in semicontinuous fermentation

scientific article published on 19 June 2017

Assessment of heterologous butyrate and butanol pathway activity by measurement of intracellular pathway intermediates in recombinant Escherichia coli

scientific article published on 13 July 2010

Biosynthesis of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose by metabolically engineered Escherichia coli

scientific article published on 14 January 2016

Carboxyl-terminated dendrimer-coated bioactive interface for protein microarray: high-sensitivity detection of antigen in complex biological samples

scientific article published on 28 March 2007

Cell free biosynthesis of isoprenoids from isopentenol

scientific article published on 03 September 2019

Characterization of lycopene-overproducing E. coli strains in high cell density fermentations

scientific article published on 25 February 2006

Co-culture engineering for microbial biosynthesis of 3-amino-benzoic acid in Escherichia coli

scientific article published on 11 May 2016

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle ⬇️

scientific article published on March 15, 2013

Combinatorial engineering of 1-deoxy-D-xylulose 5-phosphate pathway using cross-lapping in vitro assembly (CLIVA) method

scientific article

Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: identification of enzymatic bottlenecks by systematic gene overexpression

scientific article

Combining genotype improvement and statistical media optimization for isoprenoid production in E. coli

scientific article published on 04 October 2013

Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control

scientific article

Constructing an ethanol utilization pathway in Escherichia coli to produce acetyl-CoA derived compounds

scientific article published on 25 November 2020

Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets

scientific article published on 10 April 2005

Deep learning classification of lipid droplets in quantitative phase images

scientific article published on 05 April 2021

Designing a New Entry Point into Isoprenoid Metabolism by Exploiting Fructose-6-Phosphate Aldolase Side Reactivity of Escherichia coli.

scientific article published on 4 April 2017

Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements

scientific article

Development of a formaldehyde biosensor with application to synthetic methylotrophy.

scientific article

Direct evidence for cancer-cell-autonomous extracellular protein catabolism in pancreatic tumors.

scientific article published on 26 December 2016

Directed Evolution of Promoters and Tandem Gene Arrays for Customizing RNA Synthesis Rates and Regulation

scientific article published on January 1, 2011

Dissecting Mammalian Cell Metabolism through ¹³C- and ²H-Isotope Tracing: Interpretations at the Molecular and Systems Levels

scientific article published in 2019

Distributing a metabolic pathway among a microbial consortium enhances production of natural products

scientific article published on 5 January 2015

Efficient utilization of pentoses for bioproduction of the renewable two-carbon compounds ethylene glycol and glycolate

scientific article published on 19 December 2015

Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli.

scientific article published on 25 January 2016

Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions

scientific article published on 17 September 2006

Elucidation of Cellular Metabolism Via Metabolomics and Stable-Isotope Assisted Metabolomics ⬇️

scientific article published on July 1, 2011

Engineering Corynebacterium glutamicum for high-titer biosynthesis of hyaluronic acid

scientific article published on 10 July 2019

Engineering E. coli for caffeic acid biosynthesis from renewable sugars

scientific article published on 21 November 2012

Engineering E. coli-E. coli cocultures for production of muconic acid from glycerol.

scientific article

Engineering Escherichia coli coculture systems for the production of biochemical products.

scientific article published on 25 June 2015

Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals

scientific article published on 12 September 2016

Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production

scientific article published on 8 November 2016

Engineering Yarrowia lipolytica for the utilization of acid whey

scientific article published on 25 September 2019

Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol.

scientific article published on 29 July 2015

Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica.

scientific article published on 27 February 2015

Engineering microbial cell factories for biosynthesis of isoprenoid molecules: beyond lycopene

scientific article published on 2 August 2007

Engineering of Taxadiene Synthase for Improved Selectivity and Yield of a Key Taxol Biosynthetic Intermediate

scientific article published on 30 October 2016

Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae

scientific article

Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica.

scientific article published on 14 March 2017

Engineering promoter regulation

scientific article published in February 2007

Engineering yeast transcription machinery for improved ethanol tolerance and production

scientific article published in December 2006

Enhanced Biosynthesis of Hyaluronic Acid Using Engineered Corynebacterium glutamicum Via Metabolic Pathway Regulation

scientific article

Enhancing hydrogen-dependent growth of and carbon dioxide fixation by Clostridium ljungdahlii through nitrate supplementation

scientific article published on 09 December 2018

Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity

scientific article published on 03 August 2020

Enhancing solubility of deoxyxylulose phosphate pathway enzymes for microbial isoprenoid production ⬇️

scientific article published on November 14, 2012

Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation

scientific article

Erratum: Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis

article

Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells

scientific article

Evaluation of regression models in metabolic physiology: predicting fluxes from isotopic data without knowledge of the pathway

scientific article

Expanding the concepts and tools of metabolic engineering to elucidate cancer metabolism

scientific article published on 18 October 2012

Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production.

scientific article published on 26 February 2015

Exploiting Bioprocessing Fluctuations to Elicit the Mechanistics of De Novo Lipogenesis in Yarrowia lipolytica

scientific article

Exploiting biological complexity for strain improvement through systems biology

scientific article published in October 2004

Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutants

scientific article

Functional overexpression and characterization of lipogenesis-related genes in the oleaginous yeast Yarrowia lipolytica

scientific article published on 26 February 2016

Global transcription machinery engineering: a new approach for improving cellular phenotype

scientific article published on 8 January 2007

Glutaminase and poly(ADP-ribose) polymerase inhibitors suppress pyrimidine synthesis and VHL-deficient renal cancers

scientific article published on 27 March 2017

Harnessing a Methane-Fueled, Sediment-Free Mixed Microbial Community for Utilization of Distributed Sources of Natural Gas.

scientific article published on 20 February 2018

Heterologous expression and characterization of bacterial 2-C-methyl-D-erythritol-4-phosphate pathway in Saccharomyces cerevisiae

scientific article

High-throughput metabolic state analysis: the missing link in integrated functional genomics of yeasts.

scientific article published on June 2005

High-titer biosynthesis of hyaluronic acid by recombinant Corynebacterium glutamicum

scientific article published on 28 December 2015

Holistic Approaches in Lipid Production by Yarrowia lipolytica

scientific article published on 11 July 2018

Identification of gene disruptions for increased poly-3-hydroxybutyrate accumulation in Synechocystis PCC 6803.

scientific article

Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach

scientific article published in December 2005

Improving Metabolic Pathway Efficiency by Statistical Model-Based Multivariate Regulatory Metabolic Engineering.

scientific article

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

scientific article

Improving formaldehyde consumption drives methanol assimilation in engineered E. coli.

scientific article

In Vitro Metabolic Engineering of Amorpha-4,11-diene Biosynthesis at Enhanced Rate and Specific Yield of Production.

scientific article published on 18 May 2017

In vivo HIF-mediated reductive carboxylation is regulated by citrate levels and sensitizes VHL-deficient cells to glutamine deprivation

scientific article published on March 2013

Integrated bioprocess for conversion of gaseous substrates to liquids

scientific article

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli ⬇️

scientific article published on October 1, 2010

Ketogenic essential amino acids modulate lipid synthetic pathways and prevent hepatic steatosis in mice

scientific article

Key Role of the Carboxyl Terminus of Hyaluronan Synthase in Processive Synthesis and Size Control of Hyaluronic Acid Polymers.

scientific article published on 13 February 2017

L-tyrosine production by deregulated strains of Escherichia coli

scientific article published on 13 January 2007

Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p

scholarly article

Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism

scientific article published on 16 January 2017

Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis ⬇️

scientific article published on September 1, 2011

Measuring deuterium enrichment of glucose hydrogen atoms by gas chromatography/mass spectrometry

scientific article published on 17 March 2011

Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase

scientific article published on 17 December 2015

Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli

scientific article

Metabolic engineering in the host Yarrowia lipolytica

scientific article published on 26 July 2018

Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid

scientific article published on 18 September 2007

Metabolic engineering of Escherichia coli for the production of L-malate from xylose

scientific article published on 22 May 2018

Metabolic engineering of Escherichia coli for the production of isoprenoids

scientific article published on 01 May 2018

Metabolic engineering of Escherichia coli for the synthesis of the quadripolymer poly(glycolate-co-lactate-co-3-hydroxybutyrate-co-4-hydroxybutyrate) from glucose

scientific article

Metabolic flux analysis in a nonstationary system: fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol

scientific article published on 23 February 2007

Metabolic requirements for cancer cell proliferation

scientific article published on 18 August 2016

Metabolite Profiling Identified Methylerythritol Cyclodiphosphate Efflux as a Limiting Step in Microbial Isoprenoid Production ⬇️

scientific article published on November 2, 2012

Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase

scientific article

Metformin decreases glucose oxidation and increases the dependency of prostate cancer cells on reductive glutamine metabolism.

scientific article published on 17 May 2013

Method for designing and optimizing random-search libraries for strain improvement

scientific article

Mixed carbon substrates: a necessary nuisance or a missed opportunity?

scientific article published on 09 September 2019

Multi-dimensional gene target search for improving lycopene biosynthesis in Escherichia coli

scientific article published on 12 April 2007

Mutagenesis of the bacterial RNA polymerase alpha subunit for improvement of complex phenotypes

scientific article published on 27 February 2009

Nontargeted elucidation of metabolic pathways using stable-isotope tracers and mass spectrometry

scientific article

Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR ⬇️

scientific article published on April 23, 2011

Oncogenic K-Ras decouples glucose and glutamine metabolism to support cancer cell growth

scientific article

Optimization of 13C isotopic tracers for metabolic flux analysis in mammalian cells ⬇️

scientific article published on December 19, 2011

Optimization of a heterologous pathway for the production of flavonoids from glucose ⬇️

scientific article published on February 12, 2011

Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation

scientific article published on 07 April 2013

Optimization of the Isopentenol Utilization Pathway for Isoprenoid Synthesis in <i>Escherichia coli</i>

scientific article published in 2022

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis in Escherichia coli

scientific article published on 7 March 2016

Perspectives of biotechnological production of L-tyrosine and its applications

scientific article

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

scientific article published on 20 March 2019

Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis

scientific article

Profiling RNA polymerase-promoter interaction by using ssDNA-dsDNA probe on a surface addressable microarray

scientific article published on September 2007

Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis

scientific article

Pyruvate kinase isoform expression alters nucleotide synthesis to impact cell proliferation

scientific article

Quantifying carbon sources for de novo lipogenesis in wild-type and IRS-1 knockout brown adipocytes

scientific article published on 21 April 2004

Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line

scientific article published on 25 March 2008

Rational, combinatorial, and genomic approaches for engineering L-tyrosine production in Escherichia coli ⬇️

scientific article published on August 6, 2012

Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi)

scientific article published on 15 June 2018

Reductive glutamine metabolism is a function of the α-ketoglutarate to citrate ratio in cells.

scientific article published on January 2013

Response to Hammer et al.: Tuning genetic control--importance of thorough promoter characterization versus generating promoter diversity

scientific article published on 27 December 2005

Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB

scientific article

Review of metabolic pathways activated in cancer cells as determined through isotopic labeling and network analysis

scientific article published on 10 February 2017

Review of methods to probe single cell metabolism and bioenergetics

scientific article

Selection and optimization of microbial hosts for biofuels production

scientific article published on 03 July 2008

Spatially addressable protein array: ssDNA-directed assembly for antibody microarray

scientific article published in December 2007

Stabilized gene duplication enables long-term selection-free heterologous pathway expression.

scientific article published on 26 July 2009

Statistical experimental design guided optimization of a one-pot biphasic multienzyme total synthesis of amorpha-4,11-diene.

scientific article

Stimulation of MC38 tumor growth by insulin analog X10 involves the serine synthesis pathway.

scientific article

Synergistic substrate cofeeding stimulates reductive metabolism

scientific article published on 14 June 2019

Synthesis of high-titer alka(e)nes in Yarrowia lipolytica is enabled by a discovered mechanism

scientific article published on 03 December 2020

Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms

scientific article

The future of metabolic engineering and synthetic biology: towards a systematic practice

scientific article

The mTORC1 pathway stimulates glutamine metabolism and cell proliferation by repressing SIRT4

scientific journal article

The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica

scientific article published on 6 March 2015

The p85alpha regulatory subunit of phosphoinositide 3-kinase potentiates c-Jun N-terminal kinase-mediated insulin resistance

scientific article published on 5 February 2007

The phosphoinositide 3-kinase regulatory subunit p85alpha can exert tumor suppressor properties through negative regulation of growth factor signaling

scientific article published on 8 June 2010

Tuning genetic control through promoter engineering

scientific article published on 25 August 2005

Two-step pathway for isoprenoid synthesis

scientific article published on 24 December 2018

Uncovering the gene knockout landscape for improved lycopene production in E. coli

scientific article

Using biopolymer bodies for encapsulation of hydrophobic products in bacterium

scientific article published on 24 April 2020

Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae.

scientific article

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