List of works - Joelle N Pelletier

15N, 13C and 1H backbone resonance assignments of an artificially engineered TEM-1/PSE-4 class A β-lactamase chimera and its deconvoluted mutant

scientific article

2-tier bacterial and in vitro selection of active and methotrexate-resistant variants of human dihydrofolate reductase

scientific article

A direct fluorometric assay for tissue transglutaminase

scientific article

Asymmetric mutations in the tetrameric R67 dihydrofolate reductase reveal high tolerance to active-site substitutions

scientific article

Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A β-lactamase chimera

scientific article published on 10 April 2010

Biotechnological applications of transglutaminases

scientific article published on 22 October 2013

Chemical profiling of the deacetylase activity of acetyl xylan esterase A (AxeA) variants on chitooligosaccharides using hydrophilic interaction chromatography-mass spectrometry

scientific article published in 2011

Chimeric β-lactamases: global conservation of parental function and fast time-scale dynamics with increased slow motions

scientific article

Cinnamoyl inhibitors of tissue transglutaminase.

scientific article published on 27 June 2008

Combinatorial exploration of the catalytic site of a drug-resistant dihydrofolate reductase: creating alternative functional configurations

scientific article

Comparison of in vivo selection and rational design of heterodimeric coiled coils

scientific article

Computational tools for enzyme improvement: why everyone can - and should - use them.

scientific article

Development of Escherichia coli Asparaginase II for Immunosensing: A Trade-Off between Receptor Density and Sensing Efficiency

scientific article published on 17 May 2017

Dual-Target Inhibitors of the Folate Pathway Inhibit Intrinsically Trimethoprim-Resistant DfrB Dihydrofolate Reductases

scientific article published on 28 September 2020

Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements

scientific article published on 31 August 2017

Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening

scientific article

Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis

scientific article published on 10 January 2012

Expression and rapid purification of highly active hexahistidine-tagged guinea pig liver transglutaminase

scientific article

Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity.

scientific article

Fluorometric assay for tissue transglutaminase-mediated transamidation activity.

scientific article published on 18 July 2009

Fragment-based design of symmetrical bis-benzimidazoles as selective inhibitors of the trimethoprim-resistant, type II R67 dihydrofolate reductase

scientific article

General C-H Arylation Strategy for the Synthesis of Tunable Visible Light-Emitting Benzo[a]imidazo[2,1,5-c,d]indolizine Fluorophores.

scientific article published on 25 April 2017

Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling ⬇️

scientific article published on 20 May 2020

High tolerance to simultaneous active-site mutations in TEM-1 beta-lactamase: Distinct mutational paths provide more generalized beta-lactam recognition

scientific article published on January 2009

Holistic engineering of Cal-A lipase chain-length selectivity identifies triglyceride binding hot-spot

scientific article published in PLoS ONE

Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency

scientific article

Imidazolium-based ionic liquid surfaces for biosensing

scientific article published on 05 June 2013

In vitro selection for catalytic activity with ribosome display

scientific article

Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase

scientific article

Influence of the Debye length on the interaction of a small molecule-modified Au nanoparticle with a surface-bound bioreceptor

scientific article published on 01 May 2014

Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance

scientific article published on 27 February 2017

Investigation of Classical Organic and Ionic Liquid Cosolvents for Early-Stage Screening in Fragment-Based Inhibitor Design with Unrelated Bacterial and Human Dihydrofolate Reductases

scientific article published on 20 April 2017

Known Evolutionary Paths Are Accessible to Engineered ß-Lactamases Having Altered Protein Motions at the Timescale of Catalytic Turnover

scientific article published on 20 November 2020

Maintenance of native-like protein dynamics may not be required for engineering functional proteins

scientific article

Microbial transglutaminase displays broad acyl-acceptor substrate specificity

scientific article published on 25 April 2013

Miniature multi-channel SPR instrument for methotrexate monitoring in clinical samples

scientific article published on 2 October 2014

Modified peptide monolayer binding His-tagged biomolecules for small ligand screening with SPR biosensors.

scientific article

Monitoring methotrexate in clinical samples from cancer patients during chemotherapy with a LSPR-based competitive sensor

scientific article published on 01 October 2012

Multiple Conformers in Active Site of Human Dihydrofolate Reductase F31R/Q35E Double Mutant Suggest Structural Basis for Methotrexate Resistance

scientific article

Mutational 'hot-spots' in mammalian, bacterial and protozoal dihydrofolate reductases associated with antifolate resistance: sequence and structural comparison

scientific article published on February 2009

NMR investigation of Tyr105 mutants in TEM-1 beta-lactamase: dynamics are correlated with function.

scientific article published on 10 April 2007

Non-specific adsorption of crude cell lysate on surface plasmon resonance sensors

scientific article

Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure

scientific article

Peptide self-assembled monolayers for label-free and unamplified surface plasmon resonance biosensing in crude cell lysate

scientific article

Photolabeling of tissue transglutaminase reveals the binding mode of potent cinnamoyl inhibitors.

scientific article

Protein library design and screening: working out the probabilities

scientific article

Protein motions promote catalysis

scientific article

Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins

scientific article

SPR Biosensing in crude serum using ultralow fouling binary patterned peptide SAM

scientific article published on 01 May 2010

Selectively weakened binding of methotrexate by human dihydrofolate reductase allows rapid ex vivo selection of mammalian cells

scientific article published on 01 March 2011

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

scientific article

Sequence-activity relationships guide directed evolution

article

Simulated annealing exploration of an active-site tyrosine in TEM-1 beta-lactamase suggests the existence of alternate conformations

scientific article published on 01 November 2007

Site-saturation mutagenesis of Tyr-105 reveals its importance in substrate stabilization and discrimination in TEM-1 beta-lactamase

scientific article

Site-specific protein propargylation using tissue transglutaminase.

scientific article

Structure-Based Design of Dimeric Bisbenzimidazole Inhibitors to an Emergent Trimethoprim-Resistant Type II Dihydrofolate Reductase Guides the Design of Monomeric Analogues

scientific article published on 10 June 2019

The Bacterial Genomic Context of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases Highlights an Emerging Threat to Public Health

scientific article published on 13 April 2021

The C-terminal residues in the alpha-interacting domain (AID) helix anchor CaV beta subunit interaction and modulation of CaV2.3 channels

scientific article published on 26 October 2004

The Structural Dynamics of Engineered β-Lactamases Vary Broadly on Three Timescales yet Sustain Native Function

scientific article published on 30 April 2019

Tissue transglutaminase acylation: Proposed role of conserved active site Tyr and Trp residues revealed by molecular modeling of peptide substrate binding

scientific article published on 9 March 2004

Tracking Silent Hypersensitivity Reactions to Asparaginase during Leukemia Therapy Using Single-Chip Indirect Plasmonic and Fluorescence Immunosensing

scientific article published on 23 November 2017

Transglutaminase-Catalyzed Bioconjugation Using One-Pot Metal-Free Bioorthogonal Chemistry

scientific article

Will nanobiosensors change therapeutic drug monitoring? The case of methotrexate

scientific article published on 01 March 2015

List of works by Joelle N Pelletier

15N, 13C and 1H backbone resonance assignments of an artificially engineered TEM-1/PSE-4 class A β-lactamase chimera and its deconvoluted mutant

2-tier bacterial and in vitro selection of active and methotrexate-resistant variants of human dihydrofolate reductase

A direct fluorometric assay for tissue transglutaminase

Asymmetric mutations in the tetrameric R67 dihydrofolate reductase reveal high tolerance to active-site substitutions

Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A β-lactamase chimera

Biotechnological applications of transglutaminases

Chemical profiling of the deacetylase activity of acetyl xylan esterase A (AxeA) variants on chitooligosaccharides using hydrophilic interaction chromatography-mass spectrometry

Chimeric β-lactamases: global conservation of parental function and fast time-scale dynamics with increased slow motions

Cinnamoyl inhibitors of tissue transglutaminase.

Combinatorial exploration of the catalytic site of a drug-resistant dihydrofolate reductase: creating alternative functional configurations

Comparison of in vivo selection and rational design of heterodimeric coiled coils

Computational tools for enzyme improvement: why everyone can - and should - use them.

Development of Escherichia coli Asparaginase II for Immunosensing: A Trade-Off between Receptor Density and Sensing Efficiency

Dual-Target Inhibitors of the Folate Pathway Inhibit Intrinsically Trimethoprim-Resistant DfrB Dihydrofolate Reductases

Engineered, highly reactive substrates of microbial transglutaminase enable protein labeling within various secondary structure elements

Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening

Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis

Expression and rapid purification of highly active hexahistidine-tagged guinea pig liver transglutaminase

Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity.

Fluorometric assay for tissue transglutaminase-mediated transamidation activity.

Fragment-based design of symmetrical bis-benzimidazoles as selective inhibitors of the trimethoprim-resistant, type II R67 dihydrofolate reductase

General C-H Arylation Strategy for the Synthesis of Tunable Visible Light-Emitting Benzo[a]imidazo[2,1,5-c,d]indolizine Fluorophores.

Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling ⬇️

High tolerance to simultaneous active-site mutations in TEM-1 beta-lactamase: Distinct mutational paths provide more generalized beta-lactam recognition

Holistic engineering of Cal-A lipase chain-length selectivity identifies triglyceride binding hot-spot

Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency

Imidazolium-based ionic liquid surfaces for biosensing

In vitro selection for catalytic activity with ribosome display

Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase

Influence of the Debye length on the interaction of a small molecule-modified Au nanoparticle with a surface-bound bioreceptor

Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance

Investigation of Classical Organic and Ionic Liquid Cosolvents for Early-Stage Screening in Fragment-Based Inhibitor Design with Unrelated Bacterial and Human Dihydrofolate Reductases

Known Evolutionary Paths Are Accessible to Engineered ß-Lactamases Having Altered Protein Motions at the Timescale of Catalytic Turnover

Maintenance of native-like protein dynamics may not be required for engineering functional proteins

Microbial transglutaminase displays broad acyl-acceptor substrate specificity

Miniature multi-channel SPR instrument for methotrexate monitoring in clinical samples

Modified peptide monolayer binding His-tagged biomolecules for small ligand screening with SPR biosensors.

Monitoring methotrexate in clinical samples from cancer patients during chemotherapy with a LSPR-based competitive sensor

Multiple Conformers in Active Site of Human Dihydrofolate Reductase F31R/Q35E Double Mutant Suggest Structural Basis for Methotrexate Resistance

Mutational 'hot-spots' in mammalian, bacterial and protozoal dihydrofolate reductases associated with antifolate resistance: sequence and structural comparison

NMR investigation of Tyr105 mutants in TEM-1 beta-lactamase: dynamics are correlated with function.

Non-specific adsorption of crude cell lysate on surface plasmon resonance sensors

Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure

Peptide self-assembled monolayers for label-free and unamplified surface plasmon resonance biosensing in crude cell lysate

Photolabeling of tissue transglutaminase reveals the binding mode of potent cinnamoyl inhibitors.

Protein library design and screening: working out the probabilities

Protein motions promote catalysis

Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins

SPR Biosensing in crude serum using ultralow fouling binary patterned peptide SAM

Selectively weakened binding of methotrexate by human dihydrofolate reductase allows rapid ex vivo selection of mammalian cells

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

Sequence-activity relationships guide directed evolution

Simulated annealing exploration of an active-site tyrosine in TEM-1 beta-lactamase suggests the existence of alternate conformations

Site-saturation mutagenesis of Tyr-105 reveals its importance in substrate stabilization and discrimination in TEM-1 beta-lactamase

Site-specific protein propargylation using tissue transglutaminase.

Structure-Based Design of Dimeric Bisbenzimidazole Inhibitors to an Emergent Trimethoprim-Resistant Type II Dihydrofolate Reductase Guides the Design of Monomeric Analogues

The Bacterial Genomic Context of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases Highlights an Emerging Threat to Public Health

The C-terminal residues in the alpha-interacting domain (AID) helix anchor CaV beta subunit interaction and modulation of CaV2.3 channels

The Structural Dynamics of Engineered β-Lactamases Vary Broadly on Three Timescales yet Sustain Native Function

Tissue transglutaminase acylation: Proposed role of conserved active site Tyr and Trp residues revealed by molecular modeling of peptide substrate binding

Tracking Silent Hypersensitivity Reactions to Asparaginase during Leukemia Therapy Using Single-Chip Indirect Plasmonic and Fluorescence Immunosensing

Transglutaminase-Catalyzed Bioconjugation Using One-Pot Metal-Free Bioorthogonal Chemistry

Will nanobiosensors change therapeutic drug monitoring? The case of methotrexate