List of works - Junichi Takagi

2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching.

scientific article

A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein ⬇️

scientific article published on April 29, 2013

A murine monoclonal antibody that binds N-terminal extracellular segment of human protease-activated receptor-4.

scientific article

A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration.

scientific article published on January 2013

A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system

scientific article

A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP

scientific article

A systematic survey of conformational states in β1 and β4 integrins using negative-stain electron microscopy

scientific article published on 22 May 2018

Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.

scientific article published on 23 February 2016

Advances in domain and subunit localization technology for electron microscopy

scientific article published on 05 March 2019

Allosteric Inhibition of a Semaphorin 4D Receptor Plexin B1 by a High-Affinity Macrocyclic Peptide

scientific article published on 27 October 2016

An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system.

scientific article published on 14 March 2018

Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist

scientific article published in Scientific Reports

Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus.

scientific article published on 27 November 2017

Conformational Freedom of the LRP6 Ectodomain Is Regulated by N-glycosylation and the Binding of the Wnt Antagonist Dkk1.

scientific article

Corrigendum: Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors

scientific article published on 14 February 2018

Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling

scientific article

Crystal structure of a mammalian Wnt-frizzled complex

scientific article published on 29 April 2019

Crystal structure of autotaxin and insight into GPCR activation by lipid mediators

scientific article

Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor ⬇️

scientific article published on March 26, 2012

Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor

scientific article published on 30 October 2009

Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain.

scientific article published on 23 December 2010

De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist

scientific article published on 16 October 2021

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation.

scientific article published on 26 November 2009

Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy.

scientific article published on 20 November 2017

Development of a novel affinity tag system for the next generation structural biology

scientific article published on September 1, 2009

Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation

scientific article published on 23 November 2020

Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach

scientific article published on 17 June 2019

Dynamic regulation of receptor transmembrane domain assembly during signal transmission across the plasma membrane

scientific article published in August 2005

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

scientific article published on 21 June 2021

Engineering ACE2 decoy receptors to combat viral escapability

scientific article published in 2022

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1.

scientific article published on 27 June 2012

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

scientific article published on 24 May 2014

Facile Synthesis of Dimeric Thioether-Macrocyclic Peptides with Antibody-like Affinity against Plexin-B1

scientific article published on 21 May 2018

First atomic view of alpha V beta 3 integrin extracellular domain

scientific article

Functional importance of covalent homodimer of reelin protein linked via its central region

scientific article

Functional role of the Frizzled linker domain in the Wnt signaling pathway

scientific article published on 05 May 2022

Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability

scientific article

Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin

scientific article

Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors

scientific article

Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System

scientific article published on January 2017

Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer

scientific article published on 13 August 2019

Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration

scientific article published on 7 October 2015

Key interactions in integrin ectodomain responsible for global conformational change detected by elastic network normal-mode analysis

scientific article

Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins

scientific article published on 18 January 2006

Loss of Parkinson's disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c

scientific article

Lysosomal sorting of amyloid-β by the SORLA receptor is impaired by a familial Alzheimer's disease mutation

scientific article

Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor

scientific article published on 17 May 2019

Mechanistic basis for the recognition of laminin-511 by α6β1 integrin.

scientific article published on September 2017

Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation.

scientific article published on 10 April 2017

Molecular Basis of the Ligand Binding Specificity of αvβ8 Integrin

scientific article published on 31 March 2016

Molecular basis of the recognition of nephronectin by integrin alpha8beta1

scientific journal article

Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding

scientific article published on 24 June 2020

New era of structural analysis of proteins by electron microscopy-application to a dynamic structural analysis of integrin cell adhesion receptor-

scientific article published in May 2005

Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification

scientific article

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy

scientific article published in Scientific Reports

PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin

scientific article published on 28 January 2014

Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151

scientific article

Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies

scientific article

Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13.

scientific article published on 30 June 2009

Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5.

scientific article published on 30 September 2016

Schwann cell myelination requires integration of laminin activities

scientific article

Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization.

scientific article

Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin

scientific journal article

Site-specific epitope insertion into recombinant proteins using the MAP tag system

scientific article published on 09 May 2020

Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin

scientific article

Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay

scientific article published on 01 April 2019

Structural basis for amyloidogenic peptide recognition by sorLA

scientific article published on 02 February 2015

Structural basis for ligand capture and release by the endocytic receptor ApoER2.

scientific article published on 26 April 2017

Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1

scientific article

Structural basis for oligomerization of the prokaryotic peptide transporter PepT

scientific article published on 24 April 2019

Structural basis for perception of diverse chemical substances by T1r taste receptors

scientific article

Structural basis for semaphorin signalling through the plexin receptor

scientific article

Structural basis for specific recognition of reelin by its receptors

scientific article

Structural mechanism of laminin recognition by integrin

scientific article published on 29 June 2021

Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization

scientific article published on 29 December 2020

Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase

scientific article published on 18 February 2016

Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V

scientific article published in Nature Communications

Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors

scientific article

Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography

scientific article

Structure of the F-spondin reeler domain reveals a unique beta-sandwich fold with a deformable disulfide-bonded loop

scientific article

Structure of the Plexin Ectodomain Bound by Semaphorin-Mimicking Antibodies

scientific article

Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state

scientific article

The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology

scientific article published on 01 January 2018

The activated conformation of integrin β7 is a novel multiple myeloma-specific target for CAR T cell therapy

scientific article

The constitutive high affinity Met binding site in the kringle domain is dispensable for the signaling activity of hepatocyte growth factor

scientific article published on 14 January 2020

Three-dimensional architecture of virus-packed tubule

scientific article published in June 2007

Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer

scientific article

Yeast Inner-Subunit PA-NZ-1 Labeling Strategy for Accurate Subunit Identification in a Macromolecular Complex through Cryo-EM Analysis

scientific article published on 3 April 2018

[Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface].

scientific article

[Multiple discontinuous exosites for von Willebrand factor of ADAMTS13]

scientific article published on 01 October 2010

List of works by Junichi Takagi

2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching.

A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein ⬇️

A murine monoclonal antibody that binds N-terminal extracellular segment of human protease-activated receptor-4.

A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration.

A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system

A structure-based model of substrate discrimination by a noncanonical PDZ tandem in the intramembrane-cleaving protease RseP

A systematic survey of conformational states in β1 and β4 integrins using negative-stain electron microscopy

Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.

Advances in domain and subunit localization technology for electron microscopy

Allosteric Inhibition of a Semaphorin 4D Receptor Plexin B1 by a High-Affinity Macrocyclic Peptide

An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system.

Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist

Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus.

Conformational Freedom of the LRP6 Ectodomain Is Regulated by N-glycosylation and the Binding of the Wnt Antagonist Dkk1.

Corrigendum: Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors

Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling

Crystal structure of a mammalian Wnt-frizzled complex

Crystal structure of autotaxin and insight into GPCR activation by lipid mediators

Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor ⬇️

Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor

Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain.

De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation.

Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy.

Development of a novel affinity tag system for the next generation structural biology

Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation

Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach

Dynamic regulation of receptor transmembrane domain assembly during signal transmission across the plasma membrane

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

Engineering ACE2 decoy receptors to combat viral escapability

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

Facile Synthesis of Dimeric Thioether-Macrocyclic Peptides with Antibody-like Affinity against Plexin-B1

First atomic view of alpha V beta 3 integrin extracellular domain

Functional importance of covalent homodimer of reelin protein linked via its central region

Functional role of the Frizzled linker domain in the Wnt signaling pathway

Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability

Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin

Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors

Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System

Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer

Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration

Key interactions in integrin ectodomain responsible for global conformational change detected by elastic network normal-mode analysis

Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins

Loss of Parkinson's disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c

Lysosomal sorting of amyloid-β by the SORLA receptor is impaired by a familial Alzheimer's disease mutation

Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor

Mechanistic basis for the recognition of laminin-511 by α6β1 integrin.

Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation.

Molecular Basis of the Ligand Binding Specificity of αvβ8 Integrin

Molecular basis of the recognition of nephronectin by integrin alpha8beta1

Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding

New era of structural analysis of proteins by electron microscopy-application to a dynamic structural analysis of integrin cell adhesion receptor-

Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy

PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin

Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151

Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies

Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13.

Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5.

Schwann cell myelination requires integration of laminin activities

Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization.

Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin

Site-specific epitope insertion into recombinant proteins using the MAP tag system

Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin

Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay

Structural basis for amyloidogenic peptide recognition by sorLA

Structural basis for ligand capture and release by the endocytic receptor ApoER2.

Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1

Structural basis for oligomerization of the prokaryotic peptide transporter PepT

Structural basis for perception of diverse chemical substances by T1r taste receptors

Structural basis for semaphorin signalling through the plexin receptor

Structural basis for specific recognition of reelin by its receptors

Structural mechanism of laminin recognition by integrin

Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization

Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase

Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V

Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors

Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography