List of works - Ilka Wittig

5-Lipoxygenase contributes to PPARγ activation in macrophages in response to apoptotic cells

scientific article published on 13 September 2013

A Bak-dependent mitochondrial amplification step contributes to Smac mimetic/glucocorticoid-induced necroptosis

scientific article published on 11 November 2016

AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis

scientific article published on 24 October 2016

AMPK activates LXRα and ABCA1 expression in human macrophages

scientific article published on 22 June 2016

APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria

scientific article

ATP synthase deficiency due to TMEM70 mutation leads to ultrastructural mitochondrial degeneration and is amenable to treatment

scientific article published on 13 October 2015

ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry

scientific article

Accessory subunit NUYM (NDUFS4) is required for stability of the electron input module and activity of mitochondrial complex I.

scientific article published on 18 November 2016

Acyl modification and binding of mitochondrial ACP to multiprotein complexes

scientific article

Advantages and limitations of clear-native PAGE.

scientific article

An Impaired Respiratory Electron Chain Triggers Down-regulation of the Energy Metabolism and De-ubiquitination of Solute Carrier Amino Acid Transporters

scientific article published on 6 February 2016

An artificial transport metabolon facilitates improved substrate utilization in yeast

scientific article published on 4 September 2017

ArgC-Like Digestion: Complementary or Alternative to Tryptic Digestion?

scientific article published on 4 January 2017

Assembly and oligomerization of human ATP synthase lacking mitochondrial subunits a and A6L.

scientific article

Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression

scientific article published on 30 October 2014

BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4

Blue native PAGE

scientific article

Broad AOX expression in a genetically tractable mouse model does not disturb normal physiology

scientific article published on 14 December 2016

Chapter 8 Two-dimensional native electrophoresis for fluorescent and functional assays of mitochondrial complexes

scientific article

Characterization of domain interfaces in monomeric and dimeric ATP synthase

scientific article

Chronic hypoxia alters mitochondrial composition in human macrophages

scientific article published on 16 October 2013

Coi1 is a novel assembly factor of the yeast complex III-complex IV supercomplex

scientific article published on 9 August 2017

Complexome profiling identifies TMEM126B as a component of the mitochondrial complex I assembly complex

scientific article published on 13 September 2012

Conserved and species-specific molecular denominators in mammalian skeletal muscle aging

scientific article

Cystathionine γ lyase sulfhydrates the RNA binding protein HuR to preserve endothelial cell function and delay atherogenesis

article published in 2018

Cytochrome c Oxidase Biogenesis and Metallochaperone Interactions: Steps in the Assembly Pathway of a Bacterial Complex

scientific article

Cytopathic effects of the cytomegalovirus-encoded apoptosis inhibitory protein vMIA.

scientific article

Data file of a deep proteome analysis of the prefrontal cortex in aged mice with progranulin deficiency or neuronal overexpression of progranulin

scientific article

Deep proteome of human nNOS/NOS1-positive versus MOCK SH-SY5Y neuroblastoma cells under full nutrition, serum free starvation and rapamycin treatment

Degradation of the mitochondrial complex I assembly factor TMEM126B under chronic hypoxia

scientific article published on 20 February 2018

Determination of protein mobility in mitochondrial membranes of living cells

scientific article

Dicer cleavage by calpain determines platelet microRNA levels and function in diabetes

scientific article

Drug-mediated intracellular donation of nitric oxide potently inhibits 5-lipoxygenase: a possible key to future anti-leukotriene therapy

scientific article

Electrophoretic methods to isolate protein complexes from mitochondria

scientific article published on January 2007

Endo-PDI is required for TNFα-induced angiogenesis

scientific article

Exome sequencing identifies ACAD9 mutations as a cause of complex I deficiency

scientific article

Features and applications of blue-native and clear-native electrophoresis

scientific article published on October 2008

Functional assays in high-resolution clear native gels to quantify mitochondrial complexes in human biopsies and cell lines

scientific article published on November 2007

Generator-specific targets of mitochondrial reactive oxygen species

scientific article

High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes

scientific article published on 9 April 2007

Homozygous missense mutation in BOLA3 causes multiple mitochondrial dysfunctions syndrome in two siblings

scientific article published on 05 May 2012

Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I

scientific article

Hydrogen Peroxide Formation by Nox4 Limits Malignant Transformation

Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors

scientific article published on 26 January 2018

Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.

scientific article

Identification of two proteins associated with mammalian ATP synthase

scientific journal article

Inactivation of tristetraprolin in chronic hypoxia provokes the expression of cathepsin B.

scientific article

Integrin α7β1 is a redox-regulated target of hydrogen peroxide in vascular smooth muscle cell adhesion

scientific article published on 31 May 2012

Interaction of the Escherichia coli transporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units ⬇️

scientific article published on July 11, 2012

Isoform-specific interaction of C-RAF with mitochondria

scientific article

Large pore gels to separate mega protein complexes larger than 10 MDa by blue native electrophoresis: Isolation of putative respiratory strings or patches ⬇️

scientific article published on September 1, 2010

Laser-induced liquid bead ion desorption-MS of protein complexes from blue-native gels, a sensitive top-down proteomic approach

scientific article

Long Noncoding RNA MANTIS Facilitates Endothelial Angiogenic Function

scientific article published on 28 March 2017

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors

scientific article

Loss of synaptic zinc transport in progranulin deficient mice may contribute to progranulin-associated psychopathology and chronic pain

scientific article published on 15 July 2017

MTO1-deficient mouse model mirrors the human phenotype showing complex I defect and cardiomyopathy

scientific article

Mass estimation of native proteins by blue native electrophoresis: principles and practical hints

scientific article published on 20 February 2010

Methods to analyse composition and dynamics of macromolecular complexes

scientific article

Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri

scientific article published on 27 June 2011

Mitochondrial dynamics generate equal distribution but patchwork localization of respiratory Complex I

scientific article published on 01 November 2006

Mitochondrion-derived reactive oxygen species lead to enhanced amyloid beta formation

scientific article published on 28 February 2012

Molecular diagnosis in mitochondrial complex I deficiency using exome sequencing

scientific article

Mutational Analysis of the QRRQ Motif in the Yeast Hig1 Type 2 Protein Rcf1 Reveals a Regulatory Role for the Cytochrome c Oxidase Complex

scientific article published on 6 February 2017

ND3, ND1 and 39kDa subunits are more exposed in the de-active form of bovine mitochondrial complex I.

scientific article published on 21 February 2014

NOVA: a software to analyze complexome profiling data

scientific article

Native electrophoretic techniques to identify protein-protein interactions

scientific article published on December 2009

Nitric oxide contributes to protein homeostasis by S-nitrosylations of the chaperone HSPA8 and the ubiquitin ligase UBE2D

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.

scientific article published on 19 July 2011

Non-classical 2-D electrophoresis

scientific article published in January 2009

Phenotypic spectrum of eleven patients and five novel MTFMT mutations identified by exome sequencing and candidate gene screening

scientific article published on 25 December 2013

Progranulin overexpression in sensory neurons attenuates neuropathic pain in mice: Role of autophagy

scientific article published on 11 September 2016

Progranulin promotes peripheral nerve regeneration and reinnervation: role of notch signaling

scientific article published on 22 October 2016

Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve

scientific article published on 14 May 2012

Quantification of protein complexes by blue native electrophoresis

scientific article published in January 2013

Quantitative Persulfide Site Identification (qPerS-SID) Reveals Protein Targets of H2S Releasing Donors in Mammalian Cells

scientific article published on 14 July 2016

Rab7-a novel redox target that modulates inflammatory pain processing

scientific article published on 7 April 2017

Recognition of enzymes lacking bound cofactor by protein quality control

scientific article published on 12 October 2016

Redox-proteomes of human NOS1-transduced versus MOCK SH-SY5Y neuroblastoma cells under full nutrition, serum-free starvation, and rapamycin treatment

Respiratory Chain Complexes in Dynamic Mitochondria Display a Patchy Distribution in Life Cells ⬇️

scientific article published on July 30, 2010

SNO-ing at the nociceptive synapse?

scientific article published on 24 March 2011

SYNCRIP-dependent Nox2 mRNA destabilization impairs ROS formation in M2-polarized macrophages

scientific article published on 29 July 2014

Sequence-specific peptide aptamers, interacting with the intracellular domain of the epidermal growth factor receptor, interfere with Stat3 activation and inhibit the growth of tumor cells.

scientific article

Signal transducer and activator of transcription 5 (STAT5), a crucial regulator of immune and cancer cells

scientific article

Structural organization of mitochondrial ATP synthase

scientific article published on 27 April 2008

Structure of the yeast F1Fo-ATP synthase dimer and its role in shaping the mitochondrial cristae

scientific article

Subcomplexes of human ATP synthase mark mitochondrial biosynthesis disorders

Subunit mass fingerprinting of mitochondrial complex I.

scientific article

Supercomplex-associated Cox26 protein binds to cytochrome c oxidase

scientific article

Supercomplexes and subcomplexes of mitochondrial oxidative phosphorylation

scientific article published on 12 May 2006

Supramolecular organization of ATP synthase and respiratory chain in mitochondrial membranes

scientific article published on 08 January 2009

TMEM126B deficiency reduces mitochondrial SDH oxidation by LPS, attenuating HIF-1α stabilization and IL-1β expression

The LYR protein subunit NB4M/NDUFA6 of mitochondrial complex I anchors an acyl carrier protein and is essential for catalytic activity

scientific article published on 24 March 2014

The interaction of specific peptide aptamers with the DNA binding domain and the dimerization domain of the transcription factor Stat3 inhibits transactivation and induces apoptosis in tumor cells

scientific article

The mitochondrial genome of the venomous cone snail Conus consors

scientific article published in 2012

Two-dimensional native electrophoretic analysis of respiratory supercomplexes from Yarrowia lipolytica

scientific article published in May 2009

Unmasking a temperature-dependent effect of theP. anserinai-AAA protease on aging and development ⬇️

scientific article published on December 15, 2011

VCP and PSMF1: Antagonistic regulators of proteasome activity

scientific article published on 15 June 2015

sST2 translation is regulated by FGF2 via an hnRNP A1-mediated IRES-dependent mechanism.

scientific article

List of works by Ilka Wittig

5-Lipoxygenase contributes to PPARγ activation in macrophages in response to apoptotic cells

A Bak-dependent mitochondrial amplification step contributes to Smac mimetic/glucocorticoid-induced necroptosis

AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis

AMPK activates LXRα and ABCA1 expression in human macrophages

APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria

ATP synthase deficiency due to TMEM70 mutation leads to ultrastructural mitochondrial degeneration and is amenable to treatment

ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry

Accessory subunit NUYM (NDUFS4) is required for stability of the electron input module and activity of mitochondrial complex I.

Acyl modification and binding of mitochondrial ACP to multiprotein complexes

Advantages and limitations of clear-native PAGE.

An Impaired Respiratory Electron Chain Triggers Down-regulation of the Energy Metabolism and De-ubiquitination of Solute Carrier Amino Acid Transporters

An artificial transport metabolon facilitates improved substrate utilization in yeast

ArgC-Like Digestion: Complementary or Alternative to Tryptic Digestion?

Assembly and oligomerization of human ATP synthase lacking mitochondrial subunits a and A6L.

Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression

BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4

Blue native PAGE

Broad AOX expression in a genetically tractable mouse model does not disturb normal physiology

Chapter 8 Two-dimensional native electrophoresis for fluorescent and functional assays of mitochondrial complexes

Characterization of domain interfaces in monomeric and dimeric ATP synthase

Chronic hypoxia alters mitochondrial composition in human macrophages

Coi1 is a novel assembly factor of the yeast complex III-complex IV supercomplex

Complexome profiling identifies TMEM126B as a component of the mitochondrial complex I assembly complex

Conserved and species-specific molecular denominators in mammalian skeletal muscle aging

Cystathionine γ lyase sulfhydrates the RNA binding protein HuR to preserve endothelial cell function and delay atherogenesis

Cytochrome c Oxidase Biogenesis and Metallochaperone Interactions: Steps in the Assembly Pathway of a Bacterial Complex

Cytopathic effects of the cytomegalovirus-encoded apoptosis inhibitory protein vMIA.

Data file of a deep proteome analysis of the prefrontal cortex in aged mice with progranulin deficiency or neuronal overexpression of progranulin

Deep proteome of human nNOS/NOS1-positive versus MOCK SH-SY5Y neuroblastoma cells under full nutrition, serum free starvation and rapamycin treatment

Degradation of the mitochondrial complex I assembly factor TMEM126B under chronic hypoxia

Determination of protein mobility in mitochondrial membranes of living cells

Dicer cleavage by calpain determines platelet microRNA levels and function in diabetes

Drug-mediated intracellular donation of nitric oxide potently inhibits 5-lipoxygenase: a possible key to future anti-leukotriene therapy

Electrophoretic methods to isolate protein complexes from mitochondria

Endo-PDI is required for TNFα-induced angiogenesis

Exome sequencing identifies ACAD9 mutations as a cause of complex I deficiency

Features and applications of blue-native and clear-native electrophoresis

Functional assays in high-resolution clear native gels to quantify mitochondrial complexes in human biopsies and cell lines

Generator-specific targets of mitochondrial reactive oxygen species

High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes

Homozygous missense mutation in BOLA3 causes multiple mitochondrial dysfunctions syndrome in two siblings

Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I

Hydrogen Peroxide Formation by Nox4 Limits Malignant Transformation

Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors

Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.

Identification of two proteins associated with mammalian ATP synthase

Inactivation of tristetraprolin in chronic hypoxia provokes the expression of cathepsin B.

Integrin α7β1 is a redox-regulated target of hydrogen peroxide in vascular smooth muscle cell adhesion

Interaction of the Escherichia coli transporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units ⬇️

Isoform-specific interaction of C-RAF with mitochondria

Large pore gels to separate mega protein complexes larger than 10 MDa by blue native electrophoresis: Isolation of putative respiratory strings or patches ⬇️

Laser-induced liquid bead ion desorption-MS of protein complexes from blue-native gels, a sensitive top-down proteomic approach

Long Noncoding RNA MANTIS Facilitates Endothelial Angiogenic Function

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors

Loss of synaptic zinc transport in progranulin deficient mice may contribute to progranulin-associated psychopathology and chronic pain

MTO1-deficient mouse model mirrors the human phenotype showing complex I defect and cardiomyopathy

Mass estimation of native proteins by blue native electrophoresis: principles and practical hints

Methods to analyse composition and dynamics of macromolecular complexes

Mitochondrial DNA copy number and function decrease with age in the short-lived fish Nothobranchius furzeri

Mitochondrial dynamics generate equal distribution but patchwork localization of respiratory Complex I

Mitochondrion-derived reactive oxygen species lead to enhanced amyloid beta formation

Molecular diagnosis in mitochondrial complex I deficiency using exome sequencing

Mutational Analysis of the QRRQ Motif in the Yeast Hig1 Type 2 Protein Rcf1 Reveals a Regulatory Role for the Cytochrome c Oxidase Complex

ND3, ND1 and 39kDa subunits are more exposed in the de-active form of bovine mitochondrial complex I.

NOVA: a software to analyze complexome profiling data

Native electrophoretic techniques to identify protein-protein interactions

Nitric oxide contributes to protein homeostasis by S-nitrosylations of the chaperone HSPA8 and the ubiquitin ligase UBE2D

Nitric oxide-associated protein 1 (NOA1) is necessary for oxygen-dependent regulation of mitochondrial respiratory complexes.

Non-classical 2-D electrophoresis

Phenotypic spectrum of eleven patients and five novel MTFMT mutations identified by exome sequencing and candidate gene screening

Progranulin overexpression in sensory neurons attenuates neuropathic pain in mice: Role of autophagy

Progranulin promotes peripheral nerve regeneration and reinnervation: role of notch signaling

Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve

Quantification of protein complexes by blue native electrophoresis

Quantitative Persulfide Site Identification (qPerS-SID) Reveals Protein Targets of H2S Releasing Donors in Mammalian Cells

Rab7-a novel redox target that modulates inflammatory pain processing

Recognition of enzymes lacking bound cofactor by protein quality control

Redox-proteomes of human NOS1-transduced versus MOCK SH-SY5Y neuroblastoma cells under full nutrition, serum-free starvation, and rapamycin treatment

Respiratory Chain Complexes in Dynamic Mitochondria Display a Patchy Distribution in Life Cells ⬇️