List of works - Toshitsugu Fujita

A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells

scientific article

A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences

scientific article published in Scientific Reports

Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus

scientific article published on 28 July 2016

An enChIP system for the analysis of bacterial genome functions.

scientific article

Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research

scientific article

Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies

scientific article

Detection of genome-edited cells by oligoribonucleotide interference-PCR.

scientific article published in August 2018

Direct identification of insulator components by insertional chromatin immunoprecipitation

scientific article (publication date: 2011)

Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins

scientific article (publication date: 27 November 2014)

Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins

scientific article published on 5 February 2016

Functions of fasciculation and elongation protein zeta-1 (FEZ1) in the brain.

scientific article published on 17 August 2010

Gene-specific recruitment of positive and negative elongation factors during stimulated transcription of the MKP-1 gene in neuroendocrine cells.

scientific article published on 26 January 2007

Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.

scientific article

Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1

scientific article (publication date: 16 January 2004)

Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing

scientific article

Identification of physical interactions between genomic regions by enChIP-Seq.

scientific article

Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR

scientific article (publication date: 2014)

Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP).

scientific article

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.

scientific article published on 20 January 2016

Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins

scientific article

Isolation of specific genomic regions and identification of associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.

scientific article published on January 2015

Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line

scientific article

Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immnoprecipitation (iChIP)

Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation

scientific article published on 10 January 2013

Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice

scientific article published on 22 July 2019

Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR): a simple method for suppressing PCR amplification of specific DNA sequences using ORNs.

scientific article

Promoter-proximal pausing of RNA polymerase II: an opportunity to regulate gene transcription

scientific article published on 01 February 2010

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

scientific article published on 17 August 2019

Species-specific 5′-genomic structure and multiple transcription start sites in the chicken Pax5 gene ⬇️

scientific article published on January 15, 2011

The rate of c-fos transcription in vivo is continuously regulated at the level of elongation by dynamic stimulus-coupled recruitment of positive transcription elongation factor b.

scientific article published on 12 December 2006

The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner.

scientific article

Transcription elongation factors are involved in programming hormone production in pituitary neuroendocrine GH4C1 cells

scientific journal article

Transcription start sites and usage of the first exon of mouse Foxp3 gene ⬇️

scientific article published on June 22, 2012

Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.

scientific article published on 26 February 2018

Up-regulation of P-TEFb by the MEK1-extracellular signal-regulated kinase signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells

scientific journal article

enChIP systems using different CRISPR orthologues and epitope tags.

scientific article published on 27 February 2018

enChIP-Seq Analyzer: A Software Program to Analyze and Interpret enChIP-Seq Data for the Detection of Physical Interactions between Genomic Regions

scientific article published on 07 March 2022

List of works by Toshitsugu Fujita

A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells

A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences

Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus

An enChIP system for the analysis of bacterial genome functions.

Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research

Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies

Detection of genome-edited cells by oligoribonucleotide interference-PCR.

Direct identification of insulator components by insertional chromatin immunoprecipitation

Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins

Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins

Functions of fasciculation and elongation protein zeta-1 (FEZ1) in the brain.

Gene-specific recruitment of positive and negative elongation factors during stimulated transcription of the MKP-1 gene in neuroendocrine cells.

Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.

Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1

Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing

Identification of physical interactions between genomic regions by enChIP-Seq.

Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR

Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP).

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.

Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins

Isolation of specific genomic regions and identification of associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.

Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line

Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immnoprecipitation (iChIP)

Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation

Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice

Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR): a simple method for suppressing PCR amplification of specific DNA sequences using ORNs.

Promoter-proximal pausing of RNA polymerase II: an opportunity to regulate gene transcription

Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR

Species-specific 5′-genomic structure and multiple transcription start sites in the chicken Pax5 gene ⬇️

The rate of c-fos transcription in vivo is continuously regulated at the level of elongation by dynamic stimulus-coupled recruitment of positive transcription elongation factor b.

The transcription elongation factors NELF, DSIF and P-TEFb control constitutive transcription in a gene-specific manner.

Transcription elongation factors are involved in programming hormone production in pituitary neuroendocrine GH4C1 cells

Transcription start sites and usage of the first exon of mouse Foxp3 gene ⬇️

Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.

Up-regulation of P-TEFb by the MEK1-extracellular signal-regulated kinase signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells

enChIP systems using different CRISPR orthologues and epitope tags.

enChIP-Seq Analyzer: A Software Program to Analyze and Interpret enChIP-Seq Data for the Detection of Physical Interactions between Genomic Regions