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List of works by Jason Micklefield

A Flavin-Dependent Decarboxylase-Dehydrogenase-Monooxygenase Assembles the Warhead of α,β-Epoxyketone Proteasome Inhibitors.

scientific article published on 25 March 2016

A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase

scientific article

A high-throughput assay for arylamine halogenation based on a peroxidase-mediated quinone-amine coupling with applications in the screening of enzymatic halogenations.

scientific article published on 15 October 2014

A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity

scientific article published on 08 December 2011

A non-enzymatic, DNA template-directed morpholino primer extension approach.

scientific article

Active site modification of the β-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of the CDA lipopeptides

scientific article published on 06 December 2010

Active-site modifications of adenylation domains lead to hydrolysis of upstream nonribosomal peptidyl thioester intermediates.

scientific article published in April 2004

An Engineered Tryptophan Synthase Opens New Enzymatic Pathways to β-Methyltryptophan and Derivatives.

scientific article published on 22 December 2016

An Enzyme Cascade for Selective Modification of Tyrosine Residues in Structurally Diverse Peptides and Proteins

scientific article published on 11 February 2016

An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics

scientific article published on 01 March 2007

An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals

scientific article published on 08 June 2018

Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli

scientific article published on 18 July 2019

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues.

scientific article published on 11 February 2008

Backbone modification of nucleic acids: synthesis, structure and therapeutic applications.

scientific article

Bioengineering natural product biosynthetic pathways for therapeutic applications.

scientific article

Biophysical and Cellular‐Uptake Properties of Mixed‐Sequence Pyrrolidine–Amide Oligonucleotide Mimics ⬇️

scientific article published on November 11, 2011

Biotransformations in low-boiling hydrofluorocarbon solvents

scientific article published on 01 October 2004

Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms

scientific article

Chapter 14. Biosynthesis of nonribosomal peptide precursors.

scientific article published in January 2009

Daptomycin structure and mechanism of action revealed.

scientific article

De novo Biosynthesis of "Non-Natural" Thaxtomin Phytotoxins.

scientific article

Design, synthesis, conformational analysis and nucleic acid hybridisation properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM).

scientific article

Development of Halogenase Enzymes for Use in Synthesis.

scientific article published on 3 May 2017

Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase.

scientific article published on 23 August 2008

Dual transcriptional-translational cascade permits cellular level tuneable expression control.

scientific article published on 23 September 2015

Effects of Active-Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase.

scientific article published on 21 January 2016

Engineered biosynthesis of enduracidin lipoglycopeptide antibiotics using the ramoplanin mannosyltransferase Ram29.

scientific article published on 15 April 2015

Engineered biosynthesis of nonribosomal lipopeptides with modified fatty acid side chains.

scientific article published on 17 November 2007

Engineering enzymatic assembly lines to produce new antibiotics

scientific article published on 01 October 2019

Enzymatic enantioselective decarboxylative protonation of heteroaryl malonates.

scientific article published on 12 March 2015

Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes.

scientific article published on 10 April 2015

From Multistep Enzyme Monitoring to Whole-Cell Biotransformations: Development of Real-Time Ultraviolet Resonance Raman Spectroscopy

scientific article published on 27 October 2017

Functional Exchangeability of Oxidase and Dehydrogenase Reactions in the Biosynthesis of Hydroxyphenylglycine, a Nonribosomal Peptide Building Block.

scientific article published on 9 March 2015

Gene editing enables rapid engineering of complex antibiotic assembly lines

scientific article published on 25 November 2021

Generation of orthogonally selective bacterial riboswitches by targeted mutagenesis and in vivo screening

scientific article

Integrated catalysis opens new arylation pathways via regiodivergent enzymatic C-H activation.

scientific article published on 10 June 2016

Introduction of a non-natural amino acid into a nonribosomal peptide antibiotic by modification of adenylation domain specificity

scientific article published on 18 June 2012

Kinetically selective binding of single stranded RNA over DNA by a pyrrolidine-amide oligonucleotide mimic (POM).

scientific article published on April 2001

Minimum Information about a Biosynthetic Gene cluster.

scientific article published on August 2015

Mixed-sequence pyrrolidine-amide oligonucleotide mimics: Boc(Z) synthesis and DNA/RNA binding properties.

scientific article published on December 2006

Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species

scientific article

NMR confirmation that tryptophan dehydrogenation occurs with syn stereochemistry during the biosynthesis of CDA in Streptomyces coelicolor.

scientific article published on 11 October 2007

NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin.

scientific article

Nanoscale Biomolecular Structures on Self-Assembled Monolayers Generated from Modular Pegylated Disulfides

scientific article published on 14 September 2010

Nature's protection racket.

scientific article published in June 2005

Orthogonal riboswitches for tuneable coexpression in bacteria

scientific article published on 01 March 2012

Parallel scanning near-field photolithography: the snomipede.

scientific article published on November 2010

Precursor-directed biosynthesis of nonribosomal lipopeptides with modified glutamate residues

scientific article published on 29 May 2007

Probing riboswitch-ligand interactions using thiamine pyrophosphate analogues.

scientific article

Protein Micro- and Nanopatterning Using Aminosilanes with Protein-Resistant Photolabile Protecting Groups

scientific article published on 08 February 2011

RNA-selective cross-pairing of backbone-extended pyrrolidine-amide oligonucleotide mimics (bePOMs).

scientific article published in November 2007

RadH: A Versatile Halogenase for Integration into Synthetic Pathways.

scientific article

Rational Re-engineering of a Transcriptional Silencing PreQ1 Riboswitch

scientific article published on 9 July 2015

Real-Time Monitoring of Enzyme-Catalysed Reactions using Deep UV Resonance Raman Spectroscopy.

scientific article

Recent advances in engineering nonribosomal peptide assembly lines

scientific article published on 24 December 2015

Recent advances in methyltransferase biocatalysis

scientific article published on March 2017

Reengineering orthogonally selective riboswitches

scientific article

Rewiring Riboswitches to Create New Genetic Circuits in Bacteria.

scientific article

S-adenosyl-methionine-dependent methyltransferases: highly versatile enzymes in biocatalysis, biosynthesis and other biotechnological applications.

scientific article

Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles

scientific article published on 23 December 2009

Site-specific bioalkylation of rapamycin by the RapM 16-O-methyltransferase.

scientific article published on 2 March 2015

Stereochemical course of tryptophan dehydrogenation during biosynthesis of the calcium-dependent lipopeptide antibiotics.

scientific article published on 14 March 2007

Stereospecific backbone methylation of pyrrolidine-amide oligonucleotide mimics (POM)

scientific article published on 23 February 2006

Stereospecific enzymatic transformation of alpha-ketoglutarate to (2S,3R)-3-methyl glutamate during acidic lipopeptide biosynthesis.

scientific article published on 5 September 2007

Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase

scientific article published on 28 June 2018

Structure and biocatalytic scope of thermophilic flavin-dependent halogenase and flavin reductase enzymes

scientific article

Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. ⬇️

scientific article

Subsurface biomolecular imaging of Streptomyces coelicolor using secondary ion mass spectrometry.

scientific article published on 22 February 2008

The cycloaspeptides: uncovering a new model for methylated nonribosomal peptide biosynthesis.

scientific article published on 10 April 2018

Thermal bifunctionality of bacterial phenylalanine aminomutase and ammonia lyase enzymes

scientific article published on 27 March 2012

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