List of works - Tomoaki Matsuura

A 5' UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna

scientific article published on 24 May 2018

Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna

scientific article published on 15 February 2019

Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem.

scientific article published on 27 May 2015

Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA

scientific article published on 20 November 2019

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.

scientific article published on 18 October 2017

CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna

scientific article

Cell-Free Protein Synthesis inside Giant Unilamellar Vesicles Analyzed by Flow Cytometry ⬇️

scientific article published on May 22, 2012

Cell-free protein synthesis from a single copy of DNA in a glass microchamber.

scientific article published on 23 May 2012

Cell-free protein synthesis in a microchamber revealed the presence of an optimum compartment volume for high-order reactions.

scientific article published on 4 September 2013

Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions

scientific article published on 01 June 2010

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine-5'-Monophosphate

scientific article published on 22 August 2019

Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna

scientific article

Combinatorial approaches to novel proteins

scientific article published in February 2004

Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue.

scientific article

Computational design of transmembrane pores

scientific article published on 26 August 2020

Concurrent In Vitro Synthesis and Functional Detection of Nascent Activity of the KcsA Channel under a Membrane Potential

scientific article published on 22 March 2018

Constructing partial models of cells.

scientific article published on 28 April 2010

Construction and characterization of protein libraries composed of secondary structure modules

scientific article published in November 2002

Construction of a gene screening system using giant unilamellar liposomes and a fluorescence-activated cell sorter.

scientific article

Construction of an in Vitro Gene Screening System of the E. coli EmrE Transporter Using Liposome Display

scientific article

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules.

scientific article

Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

scientific article published in Nature Communications

Detection of association and fusion of giant vesicles using a fluorescence-activated cell sorter

scientific article published on 01 October 2010

Development of a bicistronic expression system in the branchiopod crustacean Daphnia magna.

scientific article published on 31 October 2017

Directed Evolution of Proteins throughIn VitroProtein Synthesis in Liposomes ⬇️

scientific article published on August 16, 2012

Early embryonic expression of a putative ecdysteroid-phosphate phosphatase in the water flea, Daphnia magna (Cladocera: Daphniidae).

scientific article published in January 2014

Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis.

scientific article published on 9 July 2012

Effects of ribosomes on the kinetics of Qβ replication.

scientific article published on 20 November 2013

Effects of symbiotic bacteria on chemical sensitivity of Daphnia magna.

scientific article published on 6 March 2017

Evolutionary molecular engineering by random elongation mutagenesis

scientific article published on 01 January 2002

Femtoliter compartment in liposomes for in vitro selection of proteins.

scientific article published on 18 July 2006

Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit.

scientific article published in May 2006

Generation of white-eyed Daphnia magna mutants lacking scarlet function

scientific article published in PLoS ONE

Genomic integration and germline transmission of plasmid injected into crustacean Daphnia magna eggs.

scientific article

Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.

scientific article

Growth evaluation method by live imaging of Daphnia magna and its application to the estimation of an insect growth regulator

scientific article published in January 2015

Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna.

scientific article

Identification of two forms of Q{beta} replicase with different thermal stabilities but identical RNA replication activity.

scientific article published on 21 September 2010

Importance of compartment formation for a self-encoding system

scientific article

Importance of parasite RNA species repression for prolonged translation-coupled RNA self-replication.

scientific article published in April 2012

In vitro directed evolution of alpha-hemolysin by liposome display

scientific article

In vitro evolution of proteins.

scientific article

In vitro evolution of α-hemolysin using a liposome display.

scientific article published on 30 September 2013

In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes.

scientific article published on 3 November 2016

In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume.

scientific article published on 11 December 2013

In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.

scientific article published on 7 March 2011

In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency

scientific article published on 28 November 2018

Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: modeling of ligand-dependent and -independent expression.

scientific article published on 25 June 2012

Kinetic analysis of the entire RNA amplification process by Qbeta replicase

scientific article published on 05 April 2007

Liposome display for in vitro selection and evolution of membrane proteins.

scientific article

Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation.

scientific article published on 2 July 2015

Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.

scientific article published on 2 November 2017

Membrane Curvature Affects the Formation of α-Hemolysin Nanopores

scientific article published on 22 April 2015

Monitoring ecdysteroid activities using genetically encoded reporter gene in Daphnia magna

scientific article published on 11 July 2018

Nascent chain, mRNA, and ribosome complexes generated by a pure translation system.

scientific article published on 17 November 2006

Optimization of mRNA design for protein expression in the crustacean Daphnia magna.

scientific article published on 2 March 2014

Origin of lognormal-like distributions with a common width in a growth and division process.

scientific article published on 16 March 2011

Population analysis of structural properties of giant liposomes by flow cytometry

scientific article published on 01 September 2009

Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch

scientific article published on 26 June 2019

Programmed vesicle fusion triggers gene expression.

scientific article

Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

scientific article published on 01 October 2018

Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction.

scientific article published in December 2008

Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling.

scientific article published on 06 February 2017

Replication of genetic information with self-encoded replicase in liposomes

scientific article published on 01 October 2008

Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection.

scientific article

Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational Modeling

scientific article published on 26 July 2018

Selection based on the folding properties of proteins with ribosome display

scientific article

Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression

scientific article published on 28 July 2021

Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna

scientific article (publication date: 2016)

Strategies for selection from protein libraries composed of de novo designed secondary structure modules.

scientific article published in February 2004

Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna.

scientific article published on 12 February 2015

Synthesis of functional proteins within liposomes.

scientific article published in January 2010

Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.

scientific article published on 28 May 2014

TALEN-mediated homologous recombination in Daphnia magna.

scientific article

TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna

scientific article

The evolutionary enhancement of genotype-phenotype linkages in the presence of multiple copies of genetic material.

scientific article published on 9 September 2014

Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia

scientific article published on 20 November 2013

α-Complementation in an artificial genome replication system in liposomes

scientific article published on 28 November 2012

List of works by Tomoaki Matsuura

A 5' UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna

Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna

Betaproteobacteria Limnohabitans strains increase fecundity in the crustacean Daphnia magna: symbiotic relationship between major bacterioplankton and zooplankton in freshwater ecosystem.

Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.

CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna

Cell-Free Protein Synthesis inside Giant Unilamellar Vesicles Analyzed by Flow Cytometry ⬇️

Cell-free protein synthesis from a single copy of DNA in a glass microchamber.

Cell-free protein synthesis in a microchamber revealed the presence of an optimum compartment volume for high-order reactions.

Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine-5'-Monophosphate

Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna

Combinatorial approaches to novel proteins

Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue.

Computational design of transmembrane pores

Concurrent In Vitro Synthesis and Functional Detection of Nascent Activity of the KcsA Channel under a Membrane Potential

Constructing partial models of cells.

Construction and characterization of protein libraries composed of secondary structure modules

Construction of a gene screening system using giant unilamellar liposomes and a fluorescence-activated cell sorter.

Construction of an in Vitro Gene Screening System of the E. coli EmrE Transporter Using Liposome Display

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules.

Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

Detection of association and fusion of giant vesicles using a fluorescence-activated cell sorter

Development of a bicistronic expression system in the branchiopod crustacean Daphnia magna.

Directed Evolution of Proteins throughIn VitroProtein Synthesis in Liposomes ⬇️

Early embryonic expression of a putative ecdysteroid-phosphate phosphatase in the water flea, Daphnia magna (Cladocera: Daphniidae).

Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis.

Effects of ribosomes on the kinetics of Qβ replication.

Effects of symbiotic bacteria on chemical sensitivity of Daphnia magna.

Evolutionary molecular engineering by random elongation mutagenesis

Femtoliter compartment in liposomes for in vitro selection of proteins.

Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit.

Generation of white-eyed Daphnia magna mutants lacking scarlet function

Genomic integration and germline transmission of plasmid injected into crustacean Daphnia magna eggs.

Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.

Growth evaluation method by live imaging of Daphnia magna and its application to the estimation of an insect growth regulator

Heterodimeric TALENs induce targeted heritable mutations in the crustacean Daphnia magna.

Identification of two forms of Q{beta} replicase with different thermal stabilities but identical RNA replication activity.

Importance of compartment formation for a self-encoding system

Importance of parasite RNA species repression for prolonged translation-coupled RNA self-replication.

In vitro directed evolution of alpha-hemolysin by liposome display

In vitro evolution of proteins.

In vitro evolution of α-hemolysin using a liposome display.

In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes.

In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume.

In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.

In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency

Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system: modeling of ligand-dependent and -independent expression.

Kinetic analysis of the entire RNA amplification process by Qbeta replicase

Liposome display for in vitro selection and evolution of membrane proteins.

Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation.

Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.

Membrane Curvature Affects the Formation of α-Hemolysin Nanopores

Monitoring ecdysteroid activities using genetically encoded reporter gene in Daphnia magna

Nascent chain, mRNA, and ribosome complexes generated by a pure translation system.

Optimization of mRNA design for protein expression in the crustacean Daphnia magna.

Origin of lognormal-like distributions with a common width in a growth and division process.

Population analysis of structural properties of giant liposomes by flow cytometry

Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch

Programmed vesicle fusion triggers gene expression.

Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer

Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction.

Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling.

Replication of genetic information with self-encoded replicase in liposomes

Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection.

Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational Modeling

Selection based on the folding properties of proteins with ribosome display

Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression

Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression

Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna

Strategies for selection from protein libraries composed of de novo designed secondary structure modules.

Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna.

Synthesis of functional proteins within liposomes.

Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system.

TALEN-mediated homologous recombination in Daphnia magna.

TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna

The evolutionary enhancement of genotype-phenotype linkages in the presence of multiple copies of genetic material.

Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia

α-Complementation in an artificial genome replication system in liposomes