List of works - Frank Hannemann

2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation.

scientific article published on 3 March 2015

A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol.

scientific article

A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase

scientific article published on 26 May 2016

A natural heme-signature variant of CYP267A1 from Sorangium cellulosum So ce56 executes diverse ω-hydroxylation.

scientific article published on 4 November 2014

A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-β-boswellic acid (KBA) based on a recombinant cytochrome P450 system.

scientific article

A new application of the yeast two-hybrid system in protein engineering.

scientific article published on 9 February 2007

A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale.

scientific article published on 25 February 2015

A solution model of the complex formed by adrenodoxin and adrenodoxin reductase determined by paramagnetic NMR spectroscopy

scientific article published on 01 August 2010

Aldosterone synthase deficiency caused by a homozygous L451F mutation in the CYP11B2 gene.

scientific article published on 21 February 2008

Alu Sx repeat-induced homozygous deletion of the StAR gene causes lipoid congenital adrenal hyperplasia ⬇️

scientific article published on January 11, 2012

An indole-deficient Escherichia coli strain improves screening of cytochromes P450 for biotechnological applications

scientific article

Application of a new versatile electron transfer system for cytochrome P450-based Escherichia coli whole-cell bioconversions ⬇️

scientific article published on December 20, 2012

Autodisplay of functional CYP106A2 in Escherichia coli.

scientific article published on 8 March 2012

Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.

scientific article published on 22 September 2017

Biotransformation of the mineralocorticoid receptor antagonists spironolactone and canrenone by human CYP11B1 and CYP11B2: Characterization of the products and their influence on mineralocorticoid receptor transactivation.

scientific article

CYP105A1 mediated 3-hydroxylation of glimepiride and glibenclamide using a recombinant Bacillus megaterium whole-cell catalyst

scientific article published on 21 December 2011

CYP109E1 is a novel versatile statin and terpene oxidase from Bacillus megaterium

scientific article published on 11 October 2017

Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli.

scientific article

Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone.

scientific article

Characterization and engineering of a carotenoid biosynthesis operon from Bacillus megaterium

scientific article published on 26 July 2018

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase.

scientific article

Characterization of the gene cluster CYP264B1-geoA from Sorangium cellulosum So ce56: biosynthesis of (+)-eremophilene and its hydroxylation.

scientific article

Cloning and expression analysis of NhL1, a gene encoding an extracellular lipase from the fungal pea pathogen Nectria haematococca MP VI (Fusarium solani f. sp. pisi) that is expressed in planta.

scientific article

Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.

scientific article

Cytochrome P450 systems--biological variations of electron transport chains.

scientific article published on 2 August 2006

Deletions in the loop surrounding the iron-sulfur cluster of adrenodoxin severely affect the interactions with its native redox partners adrenodoxin reductase and cytochrome P450(scc) (CYP11A1).

scientific article published in September 2002

Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases.

scientific article published on 28 February 2006

Direct and mediated electrochemical response of the cytochrome P450 106A2 from Bacillus megaterium ATCC 13368

scientific article published on 30 January 2012

Efficient conversion of 11-deoxycortisol to cortisol (hydrocortisone) by recombinant fission yeast Schizosaccharomyces pombe.

scientific article

Expanding the promoter toolbox of Bacillus megaterium

scientific article published on 13 February 2019

Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol

scientific article published on 27 November 2015

Five novel mutations in CYP11B2 gene detected in patients with aldosterone synthase deficiency type I: Functional characterization and structural analyses.

scientific article published on 21 May 2010

Function and engineering of the 15beta-hydroxylase CYP106A2.

scientific article

Functional characterization of Fdx1: evidence for an evolutionary relationship between P450-type and ISC-type ferredoxins.

scientific article published on 16 September 2011

Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium

scientific article published on 29 July 2015

Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.

scientific article

Genetic engineering and overexpression of ribosomal L12 protein genes from three different archaebacteria in E coli ⬇️

scientific article published on June 1, 1991

High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium

scientific article published on 15 June 2019

Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol.

scientific article

Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level.

scientific article published on 16 March 2012

Identification and circumvention of bottlenecks in CYP21A2-mediated premedrol production using recombinant Escherichia coli

scientific article published on 18 December 2019

Identification of CYP106A2 as a regioselective allylic bacterial diterpene hydroxylase

scientific article published on 26 January 2011

Intermolecular dynamics studied by paramagnetic tagging.

scientific article published on 10 March 2009

Investigation of cytochromes P450 in myxobacteria: excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56.

scientific article

Metabolism of Oral Turinabol by Human Steroid Hormone-Synthesizing Cytochrome P450 Enzymes.

scientific article published on 9 December 2015

Phenotypic, metabolic, and molecular genetic characterization of six patients with congenital adrenal hyperplasia caused by novel mutations in the CYP11B1 gene

scientific article published on 14 October 2015

Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli.

scientific article published on 22 January 2008

Regioselective Acetylation of C21 Hydroxysteroids by the Bacterial Chloramphenicol Acetyltransferase I.

scientific article

Self-association of adrenodoxin studied by using analytical ultracentrifugation

scientific article published on 17 August 2006

Structural and thermodynamic characterization of the adrenodoxin-like domain of the electron-transfer protein Etp1 from Schizosaccharomyces pombe

scientific article

The CYP11B subfamily.

scientific article

The CYPome of Sorangium cellulosum So ce56 and Identification of CYP109D1 as a New Fatty Acid Hydroxylase

scientific article published on 01 December 2010

The endogenous adrenodoxin reductase-like flavoprotein arh1 supports heterologous cytochrome P450-dependent substrate conversions in Schizosaccharomyces pombe.

scientific article published on 8 April 2008

The interaction domain of the redox protein adrenodoxin is mandatory for binding of the electron acceptor CYP11A1, but is not required for binding of the electron donor adrenodoxin reductase.

scientific article published on 22 August 2005

The interaction of bovine adrenodoxin with CYP11A1 (cytochrome P450scc) and CYP11B1 (cytochrome P45011beta ). Acceleration of reduction and substrate conversion by site-directed mutagenesis of adrenodoxin.

scientific article published on 17 July 2001

Unfolding and conformational studies on bovine adrenodoxin probed by engineered intrinsic tryptophan fluorescence.

scientific article

List of works by Frank Hannemann

2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation.

A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol.

A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase

A natural heme-signature variant of CYP267A1 from Sorangium cellulosum So ce56 executes diverse ω-hydroxylation.

A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-β-boswellic acid (KBA) based on a recombinant cytochrome P450 system.

A new application of the yeast two-hybrid system in protein engineering.

A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale.

A solution model of the complex formed by adrenodoxin and adrenodoxin reductase determined by paramagnetic NMR spectroscopy

Aldosterone synthase deficiency caused by a homozygous L451F mutation in the CYP11B2 gene.

Alu Sx repeat-induced homozygous deletion of the StAR gene causes lipoid congenital adrenal hyperplasia ⬇️

An indole-deficient Escherichia coli strain improves screening of cytochromes P450 for biotechnological applications

Application of a new versatile electron transfer system for cytochrome P450-based Escherichia coli whole-cell bioconversions ⬇️

Autodisplay of functional CYP106A2 in Escherichia coli.

Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.

Biotransformation of the mineralocorticoid receptor antagonists spironolactone and canrenone by human CYP11B1 and CYP11B2: Characterization of the products and their influence on mineralocorticoid receptor transactivation.

CYP105A1 mediated 3-hydroxylation of glimepiride and glibenclamide using a recombinant Bacillus megaterium whole-cell catalyst

CYP109E1 is a novel versatile statin and terpene oxidase from Bacillus megaterium

Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli.

Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone.

Characterization and engineering of a carotenoid biosynthesis operon from Bacillus megaterium

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase.

Characterization of the gene cluster CYP264B1-geoA from Sorangium cellulosum So ce56: biosynthesis of (+)-eremophilene and its hydroxylation.

Cloning and expression analysis of NhL1, a gene encoding an extracellular lipase from the fungal pea pathogen Nectria haematococca MP VI (Fusarium solani f. sp. pisi) that is expressed in planta.

Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.

Cytochrome P450 systems--biological variations of electron transport chains.

Deletions in the loop surrounding the iron-sulfur cluster of adrenodoxin severely affect the interactions with its native redox partners adrenodoxin reductase and cytochrome P450(scc) (CYP11A1).

Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases.

Direct and mediated electrochemical response of the cytochrome P450 106A2 from Bacillus megaterium ATCC 13368

Efficient conversion of 11-deoxycortisol to cortisol (hydrocortisone) by recombinant fission yeast Schizosaccharomyces pombe.

Expanding the promoter toolbox of Bacillus megaterium

Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol

Five novel mutations in CYP11B2 gene detected in patients with aldosterone synthase deficiency type I: Functional characterization and structural analyses.

Function and engineering of the 15beta-hydroxylase CYP106A2.

Functional characterization of Fdx1: evidence for an evolutionary relationship between P450-type and ISC-type ferredoxins.

Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium

Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.

Genetic engineering and overexpression of ribosomal L12 protein genes from three different archaebacteria in E coli ⬇️

High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium

Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol.

Human aldosterone synthase: recombinant expression in E. coli and purification enables a detailed biochemical analysis of the protein on the molecular level.

Identification and circumvention of bottlenecks in CYP21A2-mediated premedrol production using recombinant Escherichia coli

Identification of CYP106A2 as a regioselective allylic bacterial diterpene hydroxylase

Intermolecular dynamics studied by paramagnetic tagging.

Investigation of cytochromes P450 in myxobacteria: excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56.

Metabolism of Oral Turinabol by Human Steroid Hormone-Synthesizing Cytochrome P450 Enzymes.

Phenotypic, metabolic, and molecular genetic characterization of six patients with congenital adrenal hyperplasia caused by novel mutations in the CYP11B1 gene

Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli.

Regioselective Acetylation of C21 Hydroxysteroids by the Bacterial Chloramphenicol Acetyltransferase I.

Self-association of adrenodoxin studied by using analytical ultracentrifugation

Structural and thermodynamic characterization of the adrenodoxin-like domain of the electron-transfer protein Etp1 from Schizosaccharomyces pombe

The CYP11B subfamily.

The CYPome of Sorangium cellulosum So ce56 and Identification of CYP109D1 as a New Fatty Acid Hydroxylase

The endogenous adrenodoxin reductase-like flavoprotein arh1 supports heterologous cytochrome P450-dependent substrate conversions in Schizosaccharomyces pombe.

The interaction domain of the redox protein adrenodoxin is mandatory for binding of the electron acceptor CYP11A1, but is not required for binding of the electron donor adrenodoxin reductase.

The interaction of bovine adrenodoxin with CYP11A1 (cytochrome P450scc) and CYP11B1 (cytochrome P45011beta ). Acceleration of reduction and substrate conversion by site-directed mutagenesis of adrenodoxin.

Unfolding and conformational studies on bovine adrenodoxin probed by engineered intrinsic tryptophan fluorescence.