List of works - Leoš Valášek

A Unique ISR Program Determines Cellular Responses to Chronic Stress.

scientific article published in December 2017

A multifactor complex of eIF1, eIF2, eIF3, eIF5, and tRNA(i)Met promotes initiation complex assembly and couples GTP hydrolysis to AUG recognition.

scientific article

A systematic computational analysis of the rRNA-3' UTR sequence complementarity suggests a regulatory mechanism influencing post-termination events in metazoan translation

scientific article

ABCE1: A special factor that orchestrates translation at the crossroad between recycling and initiation

scientific article published on 12 May 2017

An emergency brake for protein synthesis

scientific article published on 25 April 2017

Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?

scientific article published on 26 July 2017

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

scientific article published on 14 July 2018

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle.

scientific article published on 14 September 2017

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex.

scientific article

Fail-safe mechanism of GCN4 translational control--uORF2 promotes reinitiation by analogous mechanism to uORF1 and thus secures its key role in GCN4 expression.

scientific article

Functional and biochemical characterization of human eukaryotic translation initiation factor 3 in living cells

scientific article

Functional characterization of the role of the N-terminal domain of the c/Nip1 subunit of eukaryotic initiation factor 3 (eIF3) in AUG recognition.

scientific article published on 20 June 2012

Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer

scientific article published on 19 October 2016

In vivo deletion analysis of the architecture of a multiprotein complex of translation initiation factors.

scientific article published on January 2007

In vivo evidence that eIF3 stays bound to ribosomes elongating and terminating on short upstream ORFs to promote reinitiation

scientific article published on 23 January 2017

In vivo stabilization of preinitiation complexes by formaldehyde cross-linking.

scientific article published in January 2007

In-depth analysis of cis-determinants that either promote or inhibit reinitiation on GCN4 mRNA after translation of its four short uORFs.

scientific article published on 28 January 2016

Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins.

scientific article

Please do not recycle! Translation reinitiation in microbes and higher eukaryotes.

scientific article

Polysome profile analysis--yeast

scientific article published on 01 January 2013

RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria

scientific article published in 2024

Robust heat shock induces eIF2alpha-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae.

scientific article

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

scientific article published on 12 January 2016

Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits.

scientific article published on 16 July 2001

Study of translational control of eukaryotic gene expression using yeast.

scientific article

The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.

scientific article

The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection

scientific article

The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo.

scientific article

Translation initiation factor eIF3 promotes programmed stop codon readthrough.

scientific article

Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells

scientific article (publication date: November 2013)

Yeast 18 S rRNA is directly involved in the ribosomal response to stringent AUG selection during translation initiation.

scientific article

Yeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domains

scientific article

eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA.

scientific article published on September 2008

List of works by Leoš Valášek

A Unique ISR Program Determines Cellular Responses to Chronic Stress.

A multifactor complex of eIF1, eIF2, eIF3, eIF5, and tRNA(i)Met promotes initiation complex assembly and couples GTP hydrolysis to AUG recognition.

A systematic computational analysis of the rRNA-3' UTR sequence complementarity suggests a regulatory mechanism influencing post-termination events in metazoan translation

ABCE1: A special factor that orchestrates translation at the crossroad between recycling and initiation

An emergency brake for protein synthesis

Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle.

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex.

Fail-safe mechanism of GCN4 translational control--uORF2 promotes reinitiation by analogous mechanism to uORF1 and thus secures its key role in GCN4 expression.

Functional and biochemical characterization of human eukaryotic translation initiation factor 3 in living cells

Functional characterization of the role of the N-terminal domain of the c/Nip1 subunit of eukaryotic initiation factor 3 (eIF3) in AUG recognition.

Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer

In vivo deletion analysis of the architecture of a multiprotein complex of translation initiation factors.

In vivo evidence that eIF3 stays bound to ribosomes elongating and terminating on short upstream ORFs to promote reinitiation

In vivo stabilization of preinitiation complexes by formaldehyde cross-linking.

In-depth analysis of cis-determinants that either promote or inhibit reinitiation on GCN4 mRNA after translation of its four short uORFs.

Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins.

Please do not recycle! Translation reinitiation in microbes and higher eukaryotes.

Polysome profile analysis--yeast

RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria

Robust heat shock induces eIF2alpha-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae.

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits.

Study of translational control of eukaryotic gene expression using yeast.

The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.

The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection

The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo.

Translation initiation factor eIF3 promotes programmed stop codon readthrough.

Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells

Yeast 18 S rRNA is directly involved in the ribosomal response to stringent AUG selection during translation initiation.

Yeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domains

eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA.