List of works - Kiyohiko Igarashi

"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.

scientific article

A novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose dehydrogenase

scientific article published on 21 October 2011

Activation of crystalline cellulose to cellulose III(I) results in efficient hydrolysis by cellobiohydrolase.

scientific article published on 23 February 2007

Adsorption Characteristics of Fungal Family 1 Cellulose-Binding Domain from Trichoderma reesei Cellobiohydrolase I on Crystalline Cellulose: Negative Cooperative Adsorption via a Steric Exclusion Effect ⬇️

scientific article published on September 24, 2012

Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood

scientific article

Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition ⬇️

scientific article published on April 1, 1998

Cellobiose dehydrogenase from the fungi Phanerochaete chrysosporium and Humicola insolens. A flavohemoprotein from Humicola insolens contains 6-hydroxy-FAD as the dominant active cofactor

scientific article published on 01 February 1999

Cellulose affinity purification of fusion proteins tagged with fungal family 1 cellulose-binding domain ⬇️

scientific article published on January 28, 2012

Characterization and molecular cloning of cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana.

scientific article

Characterization of carbohydrate-binding cytochrome b562 from the white-rot fungus Phanerochaete chrysosporium.

scientific article

Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium

scientific article published on 15 April 2011

Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis

scientific article published on 20 March 2012

Comparison of glycoside hydrolase family 3 β-xylosidases from basidiomycetes and ascomycetes reveals evolutionarily distinct xylan degradation systems

scientific article published on 01 February 2022

Convergent evolution of processivity in bacterial and fungal cellulases

scientific article published on 03 August 2020

Crystal Structure and Substrate Specificity Modification of Acetyl Xylan Esterase from Aspergillus luchuensis

scientific article published on 11 August 2017

Crystal Structure of Glycoside Hydrolase Family 55 -1,3-Glucanase from the Basidiomycete Phanerochaete chrysosporium

scientific article

Crystal Structure of the Catalytic and Cytochrome b Domains in a Eukaryotic Pyrroloquinoline Quinone-Dependent Dehydrogenase

scientific article published on 27 November 2019

Crystal structure of a family 6 cellobiohydrolase from the basidiomycete Phanerochaete chrysosporium

scientific article published on 17 June 2017

Crystal structure of a feruloyl esterase belonging to the tannase family: A disulfide bond near a catalytic triad

article

Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea

scientific article

Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium

scientific article

Crystallization of selenomethionyl exo-beta-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium.

scientific article

Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa.

scientific article published on 9 September 2010

Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

scientific article published on 19 September 2016

Direct electrochemistry of Phanerochaete chrysosporium cellobiose dehydrogenase covalently attached onto gold nanoparticle modified solid gold electrodes

scientific article published on 16 July 2012

Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi

scientific journal article

Effect of deglycosylation of cellobiose dehydrogenases on the enhancement of direct electron transfer with electrodes

scientific article published on 14 November 2012

Effects of xylan and starch on secretome of the basidiomycete Phanerochaete chrysosporium grown on cellulose

scientific article published on 02 June 2011

Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium.

scientific article published in June 2005

Endo-β-1,3-galactanase from Winter Mushroom Flammulina velutipes ⬇️

scientific article published on June 8, 2011

Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.

scientific article

Enzymatic synthesis of cellulose in space: gravity is a crucial factor for building cellulose II gel structure

scientific article published on 29 January 2022

Fungal PQQ-dependent dehydrogenases and their potential in biocatalysis

scientific article published on 20 December 2018

Gene cloning and heterologous expression of glycoside hydrolase family 55 beta-1,3-glucanase from the basidiomycete Phanerochaete chrysosporium

scientific article published on 01 March 2006

Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brown-rot Polyporales provides insight into mechanisms of wood decay.

scientific article

High-speed atomic force microscope combined with single-molecule fluorescence microscope.

scientific article

Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis.

scientific article published on 3 August 2017

Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium.

scientific article

Kinetics of substrate transglycosylation by glycoside hydrolase family 3 glucan (1-->3)-beta-glucosidase from the white-rot fungus Phanerochaete chrysosporium.

scientific article published in December 2004

Localization of Cellobiose Dehydrogenase in Cellulose-Grown Cultures of Phanerochaete chrysosporium ⬇️

scientific article published on April 1, 1997

Molecular cloning and characterization of a cDNA encoding cellobiose dehydrogenase from the wood-rotting fungus Grifola frondosa.

scientific article published on December 2002

Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis.

scientific article published on 25 September 2013

Production and Characterization of Recombinant Phanerochaete chrysosporium Cellobiose Dehydrogenase in the Methylotrophic Yeast Pichia pastoris ⬇️

scientific article published on September 1, 2001

Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosidase in the methylotrophic yeast Pichia pastoris.

scientific article published in January 2003

Purification, identification and molecular cloning of glycoside hydrolase family 15 glucoamylase from the brown-rot basidiomycete Fomitopsis palustris

scientific article published on 01 June 2006

Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.

scientific article published on 20 June 2008

Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose.

scientific article published on 8 September 2016

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII

scientific article

Structural analysis of β-L-arabinobiose-binding protein in the metabolic pathway of hydroxyproline-rich glycoproteins in Bifidobacterium longum

scientific article published on 04 April 2020

Structural and Biochemical Analyses of Glycoside Hydrolase Family 26 -Mannanase from a Symbiotic Protist of the Termite Reticulitermes speratus

scientific article

Surface density of cellobiohydrolase on crystalline celluloses. A critical parameter to evaluate enzymatic kinetics at a solid-liquid interface

scientific article published on 05 June 2006

Temporal alterations in the secretome of the selective ligninolytic fungus Ceriporiopsis subvermispora during growth on aspen wood reveal this organism's strategy for degrading lignocellulose.

scientific article published on 17 January 2014

The GH26 β-mannanase RsMan26H from a symbiotic protist of the termite Reticulitermes speratus is an endo-processive mannobiohydrolase: heterologous expression and characterization.

scientific article published on 27 August 2014

The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes

scientific article

The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Cel7A Is Important for Initiation of Degradation of Crystalline Cellulose ⬇️

scientific article published on March 26, 2013

The plant cell-wall enzyme AtXTH3 catalyses covalent cross-linking between cellulose and cello-oligosaccharide

scientific article published on 26 April 2017

The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose.

scientific article

The role of active site glutamate residues in catalysis of Rhodobacter capsulatus xanthine dehydrogenase.

scientific article

The use of neutron scattering to determine the functional structure of glycoside hydrolase.

scientific article published on 2 August 2016

Trade-off between processivity and hydrolytic velocity of cellobiohydrolases at the surface of crystalline cellulose

scientific article published on 14 March 2014

Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface

scientific article

Transcriptional response of the cellobiose dehydrogenase gene to cello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium

scientific article published on 9 March 2012

Two mutations convert mammalian xanthine oxidoreductase to highly superoxide-productive xanthine oxidase

scientific article

Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin.

scientific article published on 4 June 2014

Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose microcrystals

scientific article published on 01 August 1998

Unique active site and subsite features in the arabinogalactan-degrading GH43 exo-β-1,3-galactanase from Phanerochaete chrysosporium

scientific article published on 22 October 2020

Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

scientific article published on 01 January 2012

X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis

scientific article

X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium

scientific article published on 18 October 2006

List of works by Kiyohiko Igarashi

"Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography.

A novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose dehydrogenase

Activation of crystalline cellulose to cellulose III(I) results in efficient hydrolysis by cellobiohydrolase.

Adsorption Characteristics of Fungal Family 1 Cellulose-Binding Domain from Trichoderma reesei Cellobiohydrolase I on Crystalline Cellulose: Negative Cooperative Adsorption via a Steric Exclusion Effect ⬇️

Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood

Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition ⬇️

Cellobiose dehydrogenase from the fungi Phanerochaete chrysosporium and Humicola insolens. A flavohemoprotein from Humicola insolens contains 6-hydroxy-FAD as the dominant active cofactor

Cellulose affinity purification of fusion proteins tagged with fungal family 1 cellulose-binding domain ⬇️

Characterization and molecular cloning of cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana.

Characterization of carbohydrate-binding cytochrome b562 from the white-rot fungus Phanerochaete chrysosporium.

Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium

Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis

Comparison of glycoside hydrolase family 3 β-xylosidases from basidiomycetes and ascomycetes reveals evolutionarily distinct xylan degradation systems

Convergent evolution of processivity in bacterial and fungal cellulases

Crystal Structure and Substrate Specificity Modification of Acetyl Xylan Esterase from Aspergillus luchuensis

Crystal Structure of Glycoside Hydrolase Family 55 -1,3-Glucanase from the Basidiomycete Phanerochaete chrysosporium

Crystal Structure of the Catalytic and Cytochrome b Domains in a Eukaryotic Pyrroloquinoline Quinone-Dependent Dehydrogenase

Crystal structure of a family 6 cellobiohydrolase from the basidiomycete Phanerochaete chrysosporium

Crystal structure of a feruloyl esterase belonging to the tannase family: A disulfide bond near a catalytic triad

Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea

Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium

Crystallization of selenomethionyl exo-beta-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium.

Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa.

Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Direct electrochemistry of Phanerochaete chrysosporium cellobiose dehydrogenase covalently attached onto gold nanoparticle modified solid gold electrodes

Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi

Effect of deglycosylation of cellobiose dehydrogenases on the enhancement of direct electron transfer with electrodes

Effects of xylan and starch on secretome of the basidiomycete Phanerochaete chrysosporium grown on cellulose

Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium.

Endo-β-1,3-galactanase from Winter Mushroom Flammulina velutipes ⬇️

Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.

Enzymatic synthesis of cellulose in space: gravity is a crucial factor for building cellulose II gel structure

Fungal PQQ-dependent dehydrogenases and their potential in biocatalysis

Gene cloning and heterologous expression of glycoside hydrolase family 55 beta-1,3-glucanase from the basidiomycete Phanerochaete chrysosporium

Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brown-rot Polyporales provides insight into mechanisms of wood decay.

High-speed atomic force microscope combined with single-molecule fluorescence microscope.

Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis.

Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium.

Kinetics of substrate transglycosylation by glycoside hydrolase family 3 glucan (1-->3)-beta-glucosidase from the white-rot fungus Phanerochaete chrysosporium.

Localization of Cellobiose Dehydrogenase in Cellulose-Grown Cultures of Phanerochaete chrysosporium ⬇️

Molecular cloning and characterization of a cDNA encoding cellobiose dehydrogenase from the wood-rotting fungus Grifola frondosa.

Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis.

Production and Characterization of Recombinant Phanerochaete chrysosporium Cellobiose Dehydrogenase in the Methylotrophic Yeast Pichia pastoris ⬇️

Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosidase in the methylotrophic yeast Pichia pastoris.

Purification, identification and molecular cloning of glycoside hydrolase family 15 glucoamylase from the brown-rot basidiomycete Fomitopsis palustris

Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium.

Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose.

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII

Structural analysis of β-L-arabinobiose-binding protein in the metabolic pathway of hydroxyproline-rich glycoproteins in Bifidobacterium longum

Structural and Biochemical Analyses of Glycoside Hydrolase Family 26 -Mannanase from a Symbiotic Protist of the Termite Reticulitermes speratus

Surface density of cellobiohydrolase on crystalline celluloses. A critical parameter to evaluate enzymatic kinetics at a solid-liquid interface

Temporal alterations in the secretome of the selective ligninolytic fungus Ceriporiopsis subvermispora during growth on aspen wood reveal this organism's strategy for degrading lignocellulose.

The GH26 β-mannanase RsMan26H from a symbiotic protist of the termite Reticulitermes speratus is an endo-processive mannobiohydrolase: heterologous expression and characterization.

The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes

The Tryptophan Residue at the Active Site Tunnel Entrance of Trichoderma reesei Cellobiohydrolase Cel7A Is Important for Initiation of Degradation of Crystalline Cellulose ⬇️

The plant cell-wall enzyme AtXTH3 catalyses covalent cross-linking between cellulose and cello-oligosaccharide

The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose.

The role of active site glutamate residues in catalysis of Rhodobacter capsulatus xanthine dehydrogenase.

The use of neutron scattering to determine the functional structure of glycoside hydrolase.

Trade-off between processivity and hydrolytic velocity of cellobiohydrolases at the surface of crystalline cellulose

Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface

Transcriptional response of the cellobiose dehydrogenase gene to cello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium

Two mutations convert mammalian xanthine oxidoreductase to highly superoxide-productive xanthine oxidase

Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin.

Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose microcrystals

Unique active site and subsite features in the arabinogalactan-degrading GH43 exo-β-1,3-galactanase from Phanerochaete chrysosporium

Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy

X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis

X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium