List of works by Christopher Mark Smales

Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates

scientific article published on 01 November 1998

Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS

scientific article

Alternative promoters regulate cold inducible RNA-binding (CIRP) gene expression and enhance transgene expression in mammalian cells.

scientific article published on June 2013

An ultra scale-down approach identifies host cell protein differences across a panel of mAb producing CHO cell line variants

scientific article published on 18 November 2015

Application of ER Stress Biomarkers to Predict Formulated Monoclonal Antibody Stability

scientific article published on 28 June 2019

Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single-Cell Level

scientific article published on 02 May 2019

Application of microRNA Targeted 3'UTRs to Repress DHFR Selection Marker Expression for Development of Recombinant Antibody Expressing CHO Cell Pools

scientific article published on 16 July 2018

Biochemical and structural insights into bacterial organelle form and biogenesis.

scientific article

Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity.

scientific article published on 29 September 2015

Biotherapeutic protein formulation variables influence protein integrity and can promote post-translational modifications as shown using chicken egg white lysozyme as a model system.

scientific article published on 23 December 2015

Characterisation of lysozyme-steroid glucuronide conjugates

1997 doctoral thesis by Christopher Mark Smales at Massey University

Characterization of lysozyme-estrone glucuronide conjugates. The effect of the coupling reagent on the substitution level and sites of acylation

scientific article published on 01 July 1999

Characterization of therapeutic proteins by membrane and in-gel tryptic digestion

scientific article published on 01 January 2005

Cold-inducible RNA binding protein (CIRP) expression is modulated by alternative mRNAs

scientific article published on 27 April 2009

Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate.

scientific article

Comparison of the effects of 2,2,2-trifluoroethanol on peptide and protein structure and function.

scientific article

Computational modelling elucidates the mechanism of ciliary regulation in health and disease.

scientific article

Control and regulation of mRNA translation

scientific article published in February 2014

Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems.

scientific article published on July 2006

Cooling-induced SUMOylation of EXOSC10 down-regulates ribosome biogenesis.

scientific article published on 8 February 2016

Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq

scientific article published on 18 December 2019

Developments in the production of mucosal antibodies in plants.

scientific article

Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells.

scientific article published on March 2017

Engineering an improved IgG4 molecule with reduced disulfide bond heterogeneity and increased Fab domain thermal stability.

scientific article published on 18 May 2012

Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein Expression

scientific article published on 04 June 2021

Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production

scientific article published on 02 December 2019

Engineering the chaperone network of CHO cells for optimal recombinant protein production and authenticity.

scientific article

Eukaryotic Elongation Factor 2 Kinase Activity Is Required for the Phenotypes of the Rpl24 Mouse

scientific article published in 2022

Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.

scientific article published in January 2015

Evaluating data integrity in ribosome footprinting datasets through modelled polysome profiles

scientific article published in 2022

Evaluation of individual protein errors in silver-stained two-dimensional gels.

scientific article published in July 2003

Experimental and in silico modelling analyses of the gene expression pathway for recombinant antibody and by-product production in NS0 cell lines

scientific article

Expression of Trypanosoma brucei gambiense Antigens in Leishmania tarentolae. Potential for Use in Rapid Serodiagnostic Tests (RDTs)

scientific article

Global changes in gene expression observed at the transition from growth to stationary phase in Listeria monocytogenes ScottA batch culture

scientific article published on 01 January 2004

Host cell protein adsorption characteristics during protein A chromatography.

scientific article

How can thermal processing modify the antigenicity of proteins?

scientific article

Intact-Cell MALDI-ToF Mass Spectrometry for the Authentication of Drug-Adapted Cancer Cell Lines

scientific article published on 02 October 2019

Investigating variables and mechanisms that influence protein integrity in low water content amorphous carbohydrate matrices.

scientific article published in September 2009

Investigations into, and development of, a lyophilized and formulated recombinant human factor IX produced from CHO cells.

scientific article

Lysozyme conjugate immune complex formation and the effects on substrate hydrolysis.

scientific article published in May 2003

Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses

scientific article published on 15 July 2014

Mechanisms of protein modification during model anti-viral heat-treatment bioprocessing of beta-lactoglobulin variant A in the presence of sucrose

scientific article published on 01 October 2000

Meta-Analysis of Publicly Available Chinese Hamster Ovary (CHO) Cell Transcriptomic Datasets for Identifying Engineering Targets to Enhance Recombinant Protein Yields

scientific article published on 05 July 2018

Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures.

scientific article published on 6 February 2008

Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis

scientific article published on 30 September 2016

Monitoring changes in nisin susceptibility of Listeria monocytogenes Scott A as an indicator of growth phase using FACS.

scientific article published on 28 November 2005

NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural distortion upon amadori modification.

scientific article

Noncovalently linked nuclear localization peptides for enhanced calcium phosphate transfection.

scientific article published in May 2006

On the statistical analysis of the GS-NS0 cell proteome: imputation, clustering and variability testing.

scientific article published on 19 May 2006

Polysome profiling of mAb producing CHO cell lines links translational control of cell proliferation and recombinant mRNA loading onto ribosomes with global and recombinant protein synthesis.

scientific article

Post-translational events of a model reporter protein proceed with higher fidelity and accuracy upon mild hypothermic culturing of Chinese hamster ovary cells.

scientific article published in January 2010

Proceedings from the 8th PEACe Conference on Protein Expression in Animal Cells, Angra Dos Reis, Brazil, September 16-20, 2007

article

Protein modification during anti-viral heat-treatment bioprocessing of factor VIII concentrates, factor IX concentrates, and model proteins in the presence of sucrose.

scientific article published in January 2002

Protein modification during antiviral heat bioprocessing

scientific article published on 01 January 2000

Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line

scientific article published on 23 May 2016

RTN3 Is a Novel Cold-Induced Protein and Mediates Neuroprotective Effects of RBM3.

scientific article

Rapid high-throughput characterisation, classification and selection of recombinant mammalian cell line phenotypes using intact cell MALDI-ToF mass spectrometry fingerprinting and PLS-DA modelling ⬇️

scientific article

Residual on column host cell protein analysis during lifetime studies of protein A chromatography

scientific article

The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions

scientific article published on 05 June 2018

The challenges of product- and process-related impurities to an evolving biopharmaceutical industry.

scientific article published in January 2013

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

scientific article published on March 2014

The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production.

scientific article

The effect of formulation variables on protein stability and integrity of a model IgG4 monoclonal antibody and translation to formulation of a model ScFv

scientific article

The effect of peptide glycation on local secondary structure.

scientific article

The future of host cell protein (HCP) identification during process development and manufacturing linked to a risk-based management for their control.

scientific article

Transient gene expression levels from multigene expression vectors.

scientific article published on 27 January 2007

Use of ion-exchange and hydrophobic-interaction chromatography for the rapid purification of lysozyme-estrone glucuronide conjugates

scientific article published on 01 December 1994

mTORC1 signalling and eIF4E/4E-BP1 translation initiation factor stoichiometry influence recombinant protein productivity from GS-CHOK1 cells.

scientific article published on 19 October 2016

p58IPK is an inhibitor of the eIF2α kinase GCN2 and its localization and expression underpin protein synthesis and ER processing capacity

scientific article

¹H NMR spectroscopy profiling of metabolic reprogramming of Chinese hamster ovary cells upon a temperature shift during culture.

scientific article

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