List of works - J. Paul. G. Malthouse

13C NMR analysis reveals a link between L-glutamine metabolism, D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line.

scientific article published on 4 September 2003

13C NMR of cyanylated flavodoxin from Megasphaera elsdenii and of thiocyanate model compounds

scientific article published on September 1, 1992

13C NMR study of how the oxyanion pKa values of subtilisin and chymotrypsin tetrahedral adducts are affected by different amino acid residues binding in enzyme subsites S1-S4

scientific article published on 01 May 1999

13C and1H NMR Studies of Ionizations and Hydrogen Bonding in Chymotrypsin-Glyoxal Inhibitor Complexes

scientific article published in Journal of Biological Chemistry

13C- and 1H-NMR studies of oxyanion and tetrahedral intermediate stabilization by the serine proteinases: optimizing inhibitor warhead specificity and potency by studying the inhibition of the serine proteinases by peptide-derived chloromethane and

scientific article published on 01 June 2007

13C-NMR study of the inhibition of delta-chymotrypsin by a tripeptide-glyoxal inhibitor

scientific article published on March 2002

13C-n.m.r. of the cyanylated apoflavodoxin and flavodoxin from Clostridium pasteurianum.

scientific article published on August 1993

13C-n.m.r. of the cyanylated beta-lactoglobulins: evidence that Cys-121 provides the thiol group of beta-lactoglobulins A and B

scientific article published on September 1994

1H-NMb spectroscopy of beta-thiocyanatoalanine.

scientific article published on February 1996

A 13C-NMR study of azacryptand complexes

scientific article published on 01 September 2014

A 13C-NMR study of the inhibition of papain by a dipeptide-glyoxal inhibitor.

scientific article published in September 2002

A 13C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct.

scientific article published on September 1997

A 13C-n.m.r. study of cyanylated beta-lactoglobulins

scientific article published on 01 February 1994

A 1H-NMR study of the histidine resonance's of native subtilisin and of subtilisin inhibited by benzyloxycarbonylglycylglycylphenylalanyl-chloromethane

scientific article published on 01 February 1996

A comparative study of the kinetics and stereochemistry of the serine hydroxymethyltransferase- and tryptophan synthase-catalysed exchange of the pro-2R and pro-2S protons of glycine ⬇️

scientific article published on March 15, 1991

A comparison of some of the methods available for analysing the substrate dependence of the exchange of the alpha-protons of amino acids catalysed by pyridoxal-phosphate-dependent enzymes

scientific article published on 01 February 1998

A new lysine derived glyoxal inhibitor of trypsin, its properties and utilization for studying the stabilization of tetrahedral adducts by trypsin

scientific article published on 4 January 2016

A nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secreti

scientific article

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety ⬇️

scientific article published on November 1, 1975

A study of the pH dependence of the reaction of benzyloxycarbonyltryptophanylchloromethane with chymotrypsin

scientific article published on 01 February 1996

A study of the relaxation parameters of a 13C-enriched methylene carbon and a 13C-enriched perdeuteromethylene carbon attached to chymotrypsin ⬇️

scientific article published on December 15, 1991

A study of the stabilization of tetrahedral adducts by trypsin and delta-chymotrypsin ⬇️

scientific article published on September 15, 1992

A study of the stabilization of the oxyanion of tetrahedral adducts by trypsin, chymotrypsin and subtilisin.

scientific article published on April 1995

A study of the tryptophan synthase catalysed H/D exchange of the alpha-protons of amino acids

scientific article published on 01 February 1994

A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalysed exchange of the alpha-protons of amino acids--evidence for a second catalytic site

scientific article published on 01 February 1998

An NMR study of alterations in [1-13C]glucose metabolism in C6 glioma cells by gliotoxic amino acids

scientific article

An attempt to cyanylate the thiol group of bovine serum albumin with 13C-enriched cyanide and to observe the thiocyanate carbon by 13C-NMR

scientific article published on 01 February 1998

Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6.

scientific article

Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im+H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants.

scientific article published in April 1996

Claisen-type addition of glycine to pyridoxal in water.

scientific article

Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide

scientific article

Correction to Kinetic Study of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis

scientific article published on 26 May 2020

Coupling of [33S]sulphur to molybdenum(V) in different reduced forms of xanthine oxidase.

scientific article published in December 1981

Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor

scientific article

Cyanylation of the thiol of the cysteine of flavodoxin from Anabaena PCC 7119

scientific article published on 01 February 1996

Determination of the ionization state of the active-site histidine in a subtilisin-(chloromethane inhibitor) derivative by 13C-NMR.

scientific article published on July 1996

Determination of the structure of tetrahedral transition state analogues bound at the active site of chymotrypsin using 18O and 2H isotope shifts in the 13C NMR spectra of glyoxal inhibitors.

scientific article

Effect of acute dietary standardization on the urinary, plasma, and salivary metabolomic profiles of healthy humans

scientific article published on September 2006

Effect of magnetic field strength on the linewidth and spin-lattice relaxation time of the thiocyanate carbon of cyanylated beta-lactoglobulin B: optimization of the experimental parameters for observing thiocyanate carbons in proteins.

scientific article published on March 1995

Effect of the bulk solvent on the pKa values of the oxyanions of serine protease derivatives

scientific article published on 01 February 1998

Enzymatic synthesis of alpha-deuterated amino acids

scientific article published on 01 February 1996

Enzymatic synthesis of isotopically labelled serine and tryptophan for application in peptide synthesis

scientific article published on 01 September 1997

Factors affecting the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the pro-2R and pro-2S protons of glycine

scientific article published on November 1995

Gliotoxins disrupt alanine metabolism and glutathione production in C6 glioma cells: a 13C NMR spectroscopic study.

scientific article published on December 2004

Hemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin

scientific article published on 21 March 2014

Impact of the gliotoxin L-serine-O-sulphate on cellular metabolism in cultured rat astrocytes

scientific article published on 2 February 2006

Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin

scientific article published on 25 July 2012

Intramolecular Inhibition by Enzyme of Site-Specific Modification Reactions can Mask pKa Values Characteristic of the Reaction Pathway: Do the Side Chains of Aspartic Acid-158 and Lysine-156 of Papain Form an Ion-Pair? ⬇️

scientific article published on January 1, 1978

Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism

scientific article published on 20 October 2006

Ionisations within a subtilisin-glyoxal inhibitor complex

scientific article published in May 2005

Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis

scientific article published on 03 March 2020

Kinetic analysis of the exchange of the alpha-protons of amino acids by pyridoxal-phosphate-dependent enzymes.

scientific article published in February 1996

Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions.

scientific article

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the pa ⬇️

scientific article published on November 1, 1978

Molybdenum(V) e.p.r. signals obtained from xanthine oxidase on reduction with aldehyde substrates and with 2-amino-4-hydroxy-6-formylpteridine ⬇️

scientific article published on August 1, 1981

NMR Study of the Inhibition of Pepsin by Glyoxal Inhibitors: Mechanism of Tetrahedral Intermediate Stabilization by the Aspartyl Proteases†

scientific article published on 07 September 2007

NMR and alanine scan studies of glucose-dependent insulinotropic polypeptide in water

scientific article

NMR structure of the glucose-dependent insulinotropic polypeptide fragment, GIP(1–30)amide

scientific article published on 01 December 2004

Oxyanion and tetrahedral intermediate stabilisation by subtilisin: detection of a new tetrahedral adduct.

scientific article published on 21 April 2009

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe ⬇️

scientific article published on November 1, 1976

Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells.

scientific article published on February 2009

Proof that serine hydroxymethyltransferase retains its specificity for the pro-2S proton of glycine in the absence of tetrahydrofolate

scientific article published on 01 February 1996

Quantifying tetrahedral adduct formation and stabilization in the cysteine and the serine proteases

scientific article published on 11 July 2015

Quenching of beta-lactoglobulin fluorescence by 2-nitro-5-thiobenzoic acid

scientific article published on 01 February 1998

Rapid type 2 molybdenum(V) electron-paramagnetic resonance signals from xanthine oxidase and the structure of the active centre of the enzyme ⬇️

scientific article published on March 1, 1980

Stereospecificity of alpha-proton exchange reactions catalysed by pyridoxal-5'-phosphate-dependent enzymes.

scientific article

Structural and biochemical properties of LuxF from Photobacterium leiognathi

scientific article published on 21 July 2015

Studies on thiol proteinases with fluorescent probes [proceedings]

scientific article published on January 1, 1978

Substituted Pyridines as Two-Protonic-State Reactivity Probes, Reporter-Group Delivery Vehicles and Labelling Reagents for the Study of Thiol Enzymes ⬇️

scientific article published on January 1, 1978

Synthesis of 2-guanidinyl pyridines and their trypsin inhibition and docking

scientific article published on 30 June 2020

Synthesis of benzyloxycarbonylglycylglycinephenylalanine p-nitroanilide and an evaluation of its potential as a substrate for alpha-chymotrypsin and subtilisin BPN'

scientific article published on 01 February 1998

The aspartate aminotransferase-catalysed exchange of the alpha-protons of aspartate and glutamate: the effects of the R386A and R292V mutations on this exchange reaction

scientific article published on 01 September 1999

The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy

scientific article

The case for assigning a value of approximately 4 to pKaI of the essential histidine-cysteine interactive systems of papain, bromelain and ficin ⬇️

scientific article published on February 15, 1975

The effect of different amino acid side chains on the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids.

scientific article published on March 1996

The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the alpha-protons of amino acids

scientific article published on 01 July 1998

The nature of the sulphur atom liberated from xanthine oxidase by cyanide. Evidence from e.p.r. spectroscopy after 33S substitution ⬇️

scientific article published on October 1, 1980

The pyridoxal-5'-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the alpha-protons of glycine.

scientific article published in May 1998

The stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids

scientific article published on August 2004

The synthesis and characterisation of a glyoxal inhibitor of chymotrypsin

scientific article published on 01 February 1996

The use of 13C n.m.r. and saturation transfer to detect tetrahedral intermediates in reactions catalysed by chymotrypsin and also in an amide inhibitor complex

scientific article published on 01 February 1994

Using NMR as a probe of protein structure and function.

scientific article

Vinyl sulfone-based peptidomimetics as anti-trypanosomal agents: design, synthesis, biological and computational evaluation.

scientific article published on 3 September 2013

pH stability of the stromelysin-1 catalytic domain and its mechanism of interaction with a glyoxal inhibitor

scientific article published on 18 July 2011

pH-dependent spectroscopic changes associated with the hydroquinone of FMN in flavodoxins

scientific article published on 01 March 1999

List of works by J. Paul. G. Malthouse

13C NMR analysis reveals a link between L-glutamine metabolism, D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line.

13C NMR of cyanylated flavodoxin from Megasphaera elsdenii and of thiocyanate model compounds

13C NMR study of how the oxyanion pKa values of subtilisin and chymotrypsin tetrahedral adducts are affected by different amino acid residues binding in enzyme subsites S1-S4

13C and1H NMR Studies of Ionizations and Hydrogen Bonding in Chymotrypsin-Glyoxal Inhibitor Complexes

13C- and 1H-NMR studies of oxyanion and tetrahedral intermediate stabilization by the serine proteinases: optimizing inhibitor warhead specificity and potency by studying the inhibition of the serine proteinases by peptide-derived chloromethane and

13C-NMR study of the inhibition of delta-chymotrypsin by a tripeptide-glyoxal inhibitor

13C-n.m.r. of the cyanylated apoflavodoxin and flavodoxin from Clostridium pasteurianum.

13C-n.m.r. of the cyanylated beta-lactoglobulins: evidence that Cys-121 provides the thiol group of beta-lactoglobulins A and B

1H-NMb spectroscopy of beta-thiocyanatoalanine.

A 13C-NMR study of azacryptand complexes

A 13C-NMR study of the inhibition of papain by a dipeptide-glyoxal inhibitor.

A 13C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct.

A 13C-n.m.r. study of cyanylated beta-lactoglobulins

A 1H-NMR study of the histidine resonance's of native subtilisin and of subtilisin inhibited by benzyloxycarbonylglycylglycylphenylalanyl-chloromethane

A comparative study of the kinetics and stereochemistry of the serine hydroxymethyltransferase- and tryptophan synthase-catalysed exchange of the pro-2R and pro-2S protons of glycine ⬇️

A comparison of some of the methods available for analysing the substrate dependence of the exchange of the alpha-protons of amino acids catalysed by pyridoxal-phosphate-dependent enzymes

A new lysine derived glyoxal inhibitor of trypsin, its properties and utilization for studying the stabilization of tetrahedral adducts by trypsin

A nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line: metabolism of L-alanine is important to the regulation of insulin secreti

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety ⬇️

A study of the pH dependence of the reaction of benzyloxycarbonyltryptophanylchloromethane with chymotrypsin

A study of the relaxation parameters of a 13C-enriched methylene carbon and a 13C-enriched perdeuteromethylene carbon attached to chymotrypsin ⬇️

A study of the stabilization of tetrahedral adducts by trypsin and delta-chymotrypsin ⬇️

A study of the stabilization of the oxyanion of tetrahedral adducts by trypsin, chymotrypsin and subtilisin.

A study of the tryptophan synthase catalysed H/D exchange of the alpha-protons of amino acids

A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalysed exchange of the alpha-protons of amino acids--evidence for a second catalytic site

An NMR study of alterations in [1-13C]glucose metabolism in C6 glioma cells by gliotoxic amino acids

An attempt to cyanylate the thiol group of bovine serum albumin with 13C-enriched cyanide and to observe the thiocyanate carbon by 13C-NMR

Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6.

Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im+H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants.

Claisen-type addition of glycine to pyridoxal in water.

Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide

Correction to Kinetic Study of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis

Coupling of [33S]sulphur to molybdenum(V) in different reduced forms of xanthine oxidase.

Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor

Cyanylation of the thiol of the cysteine of flavodoxin from Anabaena PCC 7119

Determination of the ionization state of the active-site histidine in a subtilisin-(chloromethane inhibitor) derivative by 13C-NMR.

Determination of the structure of tetrahedral transition state analogues bound at the active site of chymotrypsin using 18O and 2H isotope shifts in the 13C NMR spectra of glyoxal inhibitors.

Effect of acute dietary standardization on the urinary, plasma, and salivary metabolomic profiles of healthy humans

Effect of magnetic field strength on the linewidth and spin-lattice relaxation time of the thiocyanate carbon of cyanylated beta-lactoglobulin B: optimization of the experimental parameters for observing thiocyanate carbons in proteins.

Effect of the bulk solvent on the pKa values of the oxyanions of serine protease derivatives

Enzymatic synthesis of alpha-deuterated amino acids

Enzymatic synthesis of isotopically labelled serine and tryptophan for application in peptide synthesis

Factors affecting the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the pro-2R and pro-2S protons of glycine

Gliotoxins disrupt alanine metabolism and glutathione production in C6 glioma cells: a 13C NMR spectroscopic study.

Hemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin

Impact of the gliotoxin L-serine-O-sulphate on cellular metabolism in cultured rat astrocytes

Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin

Intramolecular Inhibition by Enzyme of Site-Specific Modification Reactions can Mask pKa Values Characteristic of the Reaction Pathway: Do the Side Chains of Aspartic Acid-158 and Lysine-156 of Papain Form an Ion-Pair? ⬇️

Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism

Ionisations within a subtilisin-glyoxal inhibitor complex

Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis

Kinetic analysis of the exchange of the alpha-protons of amino acids by pyridoxal-phosphate-dependent enzymes.

Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions.

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the pa ⬇️

Molybdenum(V) e.p.r. signals obtained from xanthine oxidase on reduction with aldehyde substrates and with 2-amino-4-hydroxy-6-formylpteridine ⬇️

NMR Study of the Inhibition of Pepsin by Glyoxal Inhibitors: Mechanism of Tetrahedral Intermediate Stabilization by the Aspartyl Proteases†

NMR and alanine scan studies of glucose-dependent insulinotropic polypeptide in water

NMR structure of the glucose-dependent insulinotropic polypeptide fragment, GIP(1–30)amide

Oxyanion and tetrahedral intermediate stabilisation by subtilisin: detection of a new tetrahedral adduct.

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe ⬇️

Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells.

Proof that serine hydroxymethyltransferase retains its specificity for the pro-2S proton of glycine in the absence of tetrahydrofolate

Quantifying tetrahedral adduct formation and stabilization in the cysteine and the serine proteases

Quenching of beta-lactoglobulin fluorescence by 2-nitro-5-thiobenzoic acid

Rapid type 2 molybdenum(V) electron-paramagnetic resonance signals from xanthine oxidase and the structure of the active centre of the enzyme ⬇️

Stereospecificity of alpha-proton exchange reactions catalysed by pyridoxal-5'-phosphate-dependent enzymes.

Structural and biochemical properties of LuxF from Photobacterium leiognathi

Studies on thiol proteinases with fluorescent probes [proceedings]

Substituted Pyridines as Two-Protonic-State Reactivity Probes, Reporter-Group Delivery Vehicles and Labelling Reagents for the Study of Thiol Enzymes ⬇️

Synthesis of 2-guanidinyl pyridines and their trypsin inhibition and docking

Synthesis of benzyloxycarbonylglycylglycinephenylalanine p-nitroanilide and an evaluation of its potential as a substrate for alpha-chymotrypsin and subtilisin BPN'

The aspartate aminotransferase-catalysed exchange of the alpha-protons of aspartate and glutamate: the effects of the R386A and R292V mutations on this exchange reaction

The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy

The case for assigning a value of approximately 4 to pKaI of the essential histidine-cysteine interactive systems of papain, bromelain and ficin ⬇️

The effect of different amino acid side chains on the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids.

The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the alpha-protons of amino acids

The nature of the sulphur atom liberated from xanthine oxidase by cyanide. Evidence from e.p.r. spectroscopy after 33S substitution ⬇️

The pyridoxal-5'-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the alpha-protons of glycine.

The stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids