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List of works by Arne Skerra

A Fab fragment directed against the neural cell adhesion molecule L1 enhances functional recovery after injury of the adult mouse spinal cord.

scientific article

A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope.

scientific article

A Tetrahedral Boronic Acid Diester Formed by an Unnatural Amino Acid in the Ligand Pocket of an Engineered Lipocalin

scientific article published on 07 November 2019

A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand.

scientific article

A catalytic bioscavenger with improved stability and reduced susceptibility to oxidation for treatment of acute poisoning with neurotoxic organophosphorus compounds

scientific article published on 28 December 2019

A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition

scientific article

A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments

scientific article published in April 1994

A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of F(ab) fragments and leads to improved recombinant immunoglobulin stability

scientific article

A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity

scientific article

A novel method for the N-terminal modification of native proteins

scientific article published in March 2004

A photoswitchable neurotransmitter analogue bound to its receptor

scientific article

A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery

scientific article published in Scientific Reports

A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli.

scientific article

ANTICALIgN: visualizing, editing and analyzing combined nucleotide and amino acid sequence alignments for combinatorial protein engineering

scientific article

Aligning, analyzing, and visualizing sequences for antibody engineering: Automated recognition of immunoglobulin variable region features

scientific article published on 22 October 2016

Alternative Protein Scaffolds as Novel Biotherapeutics

article published in 2015

Alternative binding proteins get mature: Rivalling antibodies

article published in 2008

Alternative binding proteins: anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities

scientific article

Alternative non-antibody scaffolds for molecular recognition

scientific article

Amination of Ketones by Employing Two New (S)-Selective ω-Transaminases and the His-Tagged ω-TA from Vibrio fluvialis

article

An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies.

scholarly article

Anticalins as an alternative to antibody technology

scientific article

Anticalins directed against the fibronectin extra domain B as diagnostic tracers for glioblastomas

scientific article published on 30 September 2015

Anticalins directed against vascular endothelial growth factor receptor 3 (VEGFR-3) with picomolar affinities show potential for medical therapy and in vivo imaging

scientific article

Anticalins in drug development

scientific article published in January 2005

Anticalins small engineered binding proteins based on the lipocalin scaffold

scientific article

Anticalins: An emerging class of novel biologics to treat cancer and other severe diseases

Anticalins: exploiting a non-Ig scaffold with hypervariable loops for the engineering of binding proteins

scientific article

Anticalins: the lipocalin family as a novel protein scaffold for the development of next-generation immunotherapies

scientific article

Anticalin® Proteins as Therapeutic Agents in Human Diseases.

scientific article published in June 2018

Applications of a peptide ligand for streptavidin: the Strep-tag.

scientific article

Bacterial expression of immunoglobulin fragments

scientific article

Bacterial production and functional characterization of the Fab fragment of the murine IgG1/lambda monoclonal antibody cmHsp70.1, a reagent for tumour diagnostics

scientific article published in February 2010

Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv)

scientific article published on 01 January 1995

Binding proteins from alternative scaffolds

scientific article published in July 2004

Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes

scientific article published on 7 February 2014

Biochemical characterization of the recombinant human Nogo-A ectodomain

scientific article published on 16 April 2007

Biochemie 2009

Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore.

scientific article published on 22 February 2011

Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

scientific article published in October 1998

Colony assays for antibody fragments expressed in bacteria.

scientific article published in June 1991

Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin

scientific article

Comparative analysis reveals selective recognition of glycans by the dendritic cell receptors DC-SIGN and Langerin

scientific article

Comparative ligand-binding analysis of ten human lipocalins

scientific article

Comparison of enzymatic properties and small molecule inhibition of γ-glutamyltranspeptidases from pathogenic and commensal bacteria

scientific article published on 16 September 2016

Construction of an artificial receptor protein ("anticalin") based on the human apolipoprotein D.

scientific article

Coupled Enzymatic Alcohol-to-Amine Conversion of Isosorbide using Engineered Transaminases and Dehydrogenases

Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel

scientific article published on 15 August 2010

Crystal structure of the human odorant binding protein, OBPIIa

scientific article

Crystal structure of the sensory domain ofEscherichia coliCadC, a member of the ToxR-like protein family

scientific article

Crystal structure of the ω-aminotransferase from Paracoccus denitrificans and its phylogenetic relationship with other class III aminotransferases that have biotechnological potential

scientific article

Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity

scientific article

Crystallographic analysis of an "anticalin" with tailored specificity for fluorescein reveals high structural plasticity of the lipocalin loop region

scientific article

Current Strategies for Pharmacokinetic Optimization

DNA vaccination efficiently induces antibodies to Nogo-A and does not exacerbate experimental autoimmune encephalomyelitis

scientific article published on 16 April 2008

Deactivation of the E. coli pH stress sensor CadC by cadaverine.

scientific article published on 18 September 2012

Derivatization of antibody Fab fragments: a designer enzyme for native protein modification

scientific article

Design and characterization of a modular membrane protein anchor to functionalize the moss Physcomitrella patens with extracellular catalytic and/or binding activities

scientific article published on December 2014

Design of a surrogate Anticalin® protein directed against CD98hc for preclinical studies in mice

scientific article published on 28 May 2020

Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment

scientific article published on 23 June 2011

Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells

scientific article

Duocalins: Engineered Ligand-Binding Proteins with Dual Specificity Derived from the Lipocalin Fold ⬇️

scientific article published on September 1, 2001

Endoplasmic Reticulum Stress Response Promotes Cytotoxic Phenotype of CD8 + Intraepithelial Lymphocytes in a Mouse Model for Crohn's Disease-like Ileitis

article

Enge molekulare Erkennung von Benzo[a ]pyren durch einen hochaffinen Antikörper

Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes

scientific article published in February 1995

Engineered protein scaffolds as next-generation antibody therapeutics.

scientific article

Engineering of alanine dehydrogenase from Bacillus subtilis for novel cofactor specificity

scientific article published on 23 July 2015

Engineering of an orthogonal aminoacyl-tRNA synthetase for efficient incorporation of the non-natural amino acid O-methyl-L-tyrosine using fluorescence-based bacterial cell sorting

scientific article

Enhanced in vivo efficacy of a type I interferon superagonist with extended plasma half-life in a mouse model of multiple sclerosis

scientific article

Epitope mapping of the neuronal growth inhibitor Nogo-A for the Nogo receptor and the cognate monoclonal antibody IN-1 by means of the SPOT technique

scientific article published in May 2007

Expression and Purification of EPHA2 Tyrosine Kinase Domain for Crystallographic and NMR Studies

scientific article published on 29 September 2016

Extra-domain B in Oncofetal Fibronectin Structurally Promotes Fibrillar Head-to-tail Dimerization of Extracellular Matrix Protein

scientific article

Fermenter production of an artificial fab fragment, rationally designed for the antigen cystatin, and its optimized crystallization through constant domain shuffling.

scientific article

Filter screening of antibody Fab fragments secreted from individual bacterial colonies: Specific detection of antigen binding with a two-membrane system

article

First In-Human Medical Imaging with a PASylated 89Zr-Labeled Anti-HER2 Fab-Fragment in a Patient with Metastatic Breast Cancer

scientific article published on 20 April 2020

Fixation of gaseous CO2 by reversing a decarboxylase for the biocatalytic synthesis of the essential amino acid l-methionine

Functional characterization of a VEGF-A-targeting Anticalin, prototype of a novel therapeutic human protein class

scientific article published on 9 December 2015

Functional humanization of an anti-CD16 Fab fragment: obstacles of switching from murine {lambda} to human {lambda} or {kappa} light chains.

scientific article published on 19 November 2008

Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach.

scientific article published on December 2004

Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life

scientific article published on 26 June 2007

Generation of anticalins with specificity for a nonsymmetric phthalic acid ester

scientific article

Half-Life Extension of Therapeutic Proteins via Genetic Fusion to Recombinant PEG Mimetics

Half-life extension and non-human primate pharmacokinetic safety studies of i-body AD-114 targeting human CXCR4

scientific article published on 23 August 2019

High contrast tumor imaging with radio-labeled antibody Fab fragments tailored for optimized pharmacokinetics via PASylation

scientific article

High molecular mass radioimmunoconjugates are promising for intraperitoneal α-emitter immunotherapy due to prolonged retention in the peritoneum

scientific article published on 3 March 2012

High-affinity Anticalins with aggregation-blocking activity directed against the Alzheimer β-amyloid peptide

scientific article

High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2

scientific article

High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface

scientific article

Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp.

scientific article

Imitating the humoral immune response

scientific article

Immobilization of histidine-tagged proteins on gold surfaces using chelator thioalkanes

scientific article published on 01 February 1999

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site

scientific article

Incomplete inhibition by eculizumab: mechanistic evidence for residual C5 activity during strong complement activation

scientific article

Innenrücktitelbild: Neuprogrammierung von humanem Siderocalin zur Neutralisierung von Petrobactin, dem essenziellen Eisenfänger des Milzbrand-Bazillus (Angew. Chem. 44/2018)

Inside Back Cover: Reprogramming Human Siderocalin To Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus (Angew. Chem. Int. Ed. 44/2018)

scholarly article by Martin Dauner et al published 17 October 2018 in Angewandte Chemie International Edition

Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways

scientific article

Intracellular expression of a cloned antibody fragment interferes with hepatitis B virus surface antigen secretion

scientific article published in February 1999

Intravenous application of an anticalin dramatically lowers plasma digoxin levels and reduces its toxic effects in rats

article

Lipocalins as a scaffold

scientific article

Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins".

scientific article

Long-Acting PASylated Leptin Ameliorates Obesity by Promoting Satiety and Preventing Hypometabolism in Leptin-Deficient Lep(ob/ob) Mice

scientific article published on 22 October 2015

Molecular Design of <sup>68</sup>Ga- and <sup>89</sup>Zr-Labeled Anticalin Radioligands for PET-Imaging of PSMA-Positive Tumors

Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin

scientific article

Moos ganz groß — der PhyscoFilter

NMR Structure and Dynamics of the Engineered Fluorescein-Binding Lipocalin FluA Reveal Rigidification of β-Barrel and Variable Loops upon Enthalpy-Driven Ligand Binding

scientific article

NMR structure of lipoprotein YxeF from Bacillus subtilis reveals a calycin fold and distant homology with the lipocalin Blc from Escherichia coli

scientific article

Neuartige biopharmazeutische Wirkstoffe auf der Grundlage von Lipocalinen

Neuprogrammierung von humanem Siderocalin zur Neutralisierung von Petrobactin, dem essenziellen Eisenfänger des Milzbrand-Bazillus

Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition

scientific article

New insights into the signaling mechanism of the pH-responsive, membrane-integrated transcriptional activator CadC of Escherichia coli

scientific article published on 7 January 2011

Non-Antibody Scaffolds

Non-Antibody Scaffolds as Alternative Therapeutic Agents

Novel Monoclonal Antibodies Recognizing Human Prostate-Specific Membrane Antigen (PSMA) as Research and Theranostic Tools.

scientific article published in March 2017

Novel serial positive enrichment technology enables clinical multiparameter cell sorting

scientific article (publication date: 2012)

One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

scientific article

Optimisation of a system for the co-translational incorporation of a keto amino acid and its application to a tumour-specific Anticalin

scientific article published on 23 September 2015

PAS-cal: A repetitive peptide sequence calibration standard for MALDI mass spectrometry

scientific article

PAS-cal: a generic recombinant peptide calibration standard for mass spectrometry

scientific article

PASylated Coversin, a C5-Specific Complement Inhibitor with Extended Pharmacokinetics, Shows Enhanced Anti-Hemolytic Activity in Vitro

scientific article

PASylated Thymosin α1: A Long-Acting Immunostimulatory Peptide for Applications in Oncology and Virology

scientific article published on 24 December 2020

PASylation of IL-1 receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis

scientific article published on 09 December 2019

PASylation of Murine Leptin Leads to Extended Plasma Half-Life and Enhanced in Vivo Efficacy

PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins

scientific article published on 10 June 2013

Periplasmic chaperones used to enhance functional secretion of proteins in E. coli

scientific article published on January 2011

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

scientific article published on July 1992

Proline/alanine-rich sequence (PAS) polypeptides as an alternative to PEG precipitants for protein crystallization

scientific article published on 01 July 2020

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

article

Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination

article

Rational Design of an Anticalin-Type Sugar-Binding Protein Using a Genetically Encoded Boronate Side Chain.

scientific article

Rational engineering of a fluorescein-binding anticalin for improved ligand affinity

scientific article published in November 2005

Regeneration of auditory nerve following complete sectioning and intrathecal application of the IN-1 antibody

scientific article published in February 2002

Reprogramming Human Siderocalin To Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus

scholarly article by Martin Dauner et al published 2 October 2018 in Angewandte Chemie

Reprogramming the body weight set point by a reciprocal interaction of hypothalamic leptin sensitivity and Pomc gene expression reverts extreme obesity

scientific article published on 05 August 2016

Resonance assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA.

scientific article published in October 2003

Scavenging Bacterial Siderophores with Engineered Lipocalin Proteins as an Alternative Antimicrobial Strategy

scientific article published on 13 December 2019

Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines ⬇️

scientific article published on December 1, 1991

Selection and characterization of Anticalins targeting human prostate-specific membrane antigen (PSMA).

scientific article published on 21 January 2016

Sequence analysis and bacterial production of the anti-c-myc antibody 9E10: the V(H) domain has an extended CDR-H3 and exhibits unusual solubility

scientific article published on September 1997

Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine.

scientific article

Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold.

scientific article

Solubility engineering and crystallization of human apolipoprotein D.

scientific article published on December 2005

Sortase-catalyzed in vitro functionalization of a HER2-specific recombinant Fab for tumor targeting of the plant cytotoxin gelonin

scientific article published on 09 December 2013

Stability engineering of the Geobacillus stearothermophilus alcohol dehydrogenase and application for the synthesis of a polyamide 12 precursor

scientific article published on 2 September 2015

Structural Basis for the Enzymatic Formation of the Key Strawberry Flavor Compound 4-Hydroxy-2,5-dimethyl-3(2H)-furanone

scientific article

Structural Basis for the Interconversion of Maltodextrins by MalQ, the Amylomaltase of Escherichia coli.

scientific article

Structural Basis for the Specific Cotranslational Incorporation of p-Boronophenylalanine into Biosynthetic Proteins.

scientific article published on 18 April 2018

Structural analysis of the L-alanoyl-D-glutamate endopeptidase domain of Listeria bacteriophage endolysin Ply500 reveals a new member of the LAS peptidase family

scientific article

Structural and biochemical analyses reveal a monomeric state of the bacterial lipocalin Blc

scientific article

Structural basis for cellulose binding by the type A carbohydrate-binding module 64 of Spirochaeta thermophila

scientific article

Structural differences between the ectodomains of murine and human CD98hc

scientific article published on 23 April 2019

Structural features of the McPC603 Fab fragment not defined in the X-ray structure.

scientific article

Structural insight into the dual ligand specificity and mode of high density lipoprotein association of apolipoprotein D

scientific article

Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein

scientific article

Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin

scientific article

Structure of lumazine protein, an optical transponder of luminescent bacteria

scientific article

Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

scientific article

Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture.

scientific article

Structure-guided engineering of Anticalins with improved binding behavior and biochemical characteristics for application in radio-immuno imaging and/or therapy

scientific article

Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody

scientific article

Temperature and pressure dependence of protein stability: the engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram

scholarly article

The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin

scientific article

The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands

scientific journal article

The Escherichia coli-derived Fab fragment of the IgM/kappa antibody IN-1 recognizes and neutralizes myelin-associated inhibitors of neurite growth

scientific article

The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture

scientific article published in January 2015

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

scientific article

The bilin-binding protein of Pieris brassicae. cDNA sequence and regulation of expression reveal distinct features of this insect pigment protein

scientific article published on February 1994

The carbohydrate recognition domain of Langerin reveals high structural similarity with the one of DC-SIGN but an additional, calcium-independent sugar-binding site

scientific article

The crystal structure of human α(1)-microglobulin reveals a potential haem-binding site

scientific article

The crystal structure of the bacteriophage PSA endolysin reveals a unique fold responsible for specific recognition of Listeria cell walls

scientific article

The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins

scientific article

The dimeric crystal structure of the human fertility lipocalin glycodelin reveals a protein scaffold for the presentation of complex glycans

scientific article

The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique.

scientific article published in March 1991

The menagerie of human lipocalins: a natural protein scaffold for molecular recognition of physiological compounds

scientific article

The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization

scientific article published in February 2004

The polypeptide biophysics of proline/alanine-rich sequences (PAS): Recombinant biopolymers with PEG-like properties

scientific article

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

scientific article published on January 1993

The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab fragment

scientific article

The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops

scientific article

Tight Molecular Recognition of Benzo[a]pyrene by a High-Affinity Antibody

scientific article

Translating the Concept of Bispecific Antibodies to Engineering Heterodimeric Phosphotriesterases with Broad Organophosphate Substrate Recognition

scientific article published on 04 November 2020

Treatment of diet-induced lipodystrophic C57BL/6J mice with long-acting PASylated leptin normalises insulin sensitivity and hepatic steatosis by promoting lipid utilisation

scientific article published on 7 June 2016

Tumor Uptake of Anti-CD20 Fabs Depends on Tumor Perfusion

scientific article published on 14 July 2016

Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach

scientific article published in May 2002

Type I IFN signaling blockade by a PASylated antagonist during chronic SIV infection suppresses specific inflammatory pathways but does not alter T cell activation or virus replication

scientific article published on 24 August 2018

Ultrafast electron transfer in the complex between fluorescein and a cognate engineered lipocalin protein, a so-called anticalin

scientific article published in March 2002

Use of Thiophilic Adsorption Chromatography for the One-Step Purification of a Bacterially Produced Antibody Fab Fragment without the Need for an Affinity Tag

article by Markus Fiedler & Arne Skerra published December 1999 in Protein Expression and Purification

Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates

scientific article published on 01 September 1998

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

article

[34] Expression of functional antibody Fv and Fab fragments in Escherichia coli

scientific article published on 01 January 1989

proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermenter production of a recombinant antibody fragment

scientific article published in August 2001

β-Barrel scaffolds for the grafting of extracellular loops from G-protein-coupled receptors

scientific article

⁸⁹Zr-Labeled Versus ¹²⁴I-Labeled αHER2 Fab with Optimized Plasma Half-Life for High-Contrast Tumor Imaging In Vivo

scientific article

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