List of works - Nico Smit

Automated Multiplex LC-MS/MS Assay for Quantifying Serum Apolipoproteins A-I, B, C-I, C-II, C-III, and E with Qualitative Apolipoprotein E Phenotyping

scientific article published on 19 November 2015

Biochemical characterization of a new melanoma model--the minipig MeLiM strain

scientific article published on 01 December 2003

Calcineurin activity and inhibition in skin and (epi)dermal cell cultures.

scientific article

Catechol-O-methyltransferase in vitiligo

scientific article published on 01 January 1994

Coat color dilution in mice because of inactivation of the melanoma antigen MART-1

scientific article published on 07 October 2011

Cytotoxic interactions of Zn2+ in vitro: melanoma cells are more susceptible than melanocytes

scientific article published on 01 December 1997

Cytotoxicity of a selected series of substituted phenols towards cultured melanoma cells

scientific article published on December 1, 1992

Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands

scientific article published on 01 September 2018

Determination of catechol O-methyltransferase activity in relation to melanin metabolism using high-performance liquid chromatography with fluorimetric detection

scientific article published on 01 November 1990

Determination of pheomelanin by measurement of aminohydroxyphenylalanine isomers with high-performance liquid chromatography.

scientific article published on October 1997

Disturbed melanin synthesis and chronic oxidative stress in dysplastic naevi

scientific article published on 01 June 2004

Effects of arsenite and UVA-1 radiation on calcineurin signaling.

scientific article

Effects of ultraviolet A-1 radiation on calcineurin activity and cytokine production in (skin) cell cultures

Evaluation of interspecimen trypsin digestion efficiency prior to multiple reaction monitoring-based absolute protein quantification with native protein calibrators.

scientific article published on 13 November 2013

Everolimus and sirolimus antagonize tacrolimus based calcineurin inhibition via competition for FK-binding protein 12.

scientific article published on 30 December 2008

Evidence of the indirect formation of the catecholic intermediate substrate responsible for the autoactivation kinetics of tyrosinase.

scientific article published in October 1997

Glutamate receptors on human melanocytes regulate the expression of MiTF.

scientific article published in February 2006

Increased melanogenesis is a risk factor for oxidative DNA damage--study on cultured melanocytes and atypical nevus cells.

scientific article published in May 2008

Keratinocytes control the pheo/eumelanin ratio in cultured normal human melanocytes

scientific article published on 01 December 2002

Large-scale cultivation of human melanocytes using collagen-coated Sephadex beads (cytodex 3)

scientific article published on 01 January 1989

Ligand-dependent activation of the melanocortin 5 receptor: cAMP production and ryanodine receptor-dependent elevations of [Ca(2+)](I).

scientific article published on January 2002

Loss of adhesion to basement membrane components but not to keratinocytes in proliferating melanocytes

scientific article published on 01 May 1996

MS-based proteomics: a metrological sound and robust alternative for apolipoprotein E phenotyping in a multiplexed test

scientific article published on 01 April 2019

Melanin content of cultured human melanocytes and UV-induced cytotoxicity.

scientific article published on August 2001

Melanin has a role in Ca2+ homeostasis in human melanocytes.

scientific article published in April 2003

Melanogenesis in cultured melanocytes can be substantially influenced by L-tyrosine and L-cysteine

scientific article published on 01 December 1997

Melanogenesis in transfected fibroblasts induces lysosomal activation.

scientific article published on February 1997

Melanosomal pH controls rate of melanogenesis, eumelanin/phaeomelanin ratio and melanosome maturation in melanocytes and melanoma cells.

scientific article

Metabolic interference of melanogenesis in pigment cells.

scientific article published on January 1996

Metrological traceability and harmonization of medical tests: a quantum leap forward is needed to keep pace with globalization and stringent IVD-regulations in the 21st century!

scientific article published on 01 September 2018

Metrological traceability in mass spectrometry-based targeted protein quantitation: a proof-of-principle study for serum apolipoproteins A-I and B100

scientific article published on 25 June 2014

Molecular diagnostics of calcineurin-related pathologies.

scientific article published on 20 October 2011

O-methylation of L-dopa in melanin metabolism and the presence of catechol-O-methyltransferase in melanocytes

scientific article published in December 1994

Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers.

scientific article

Properties and genetic control of anthocyanin 5-O-glucosyltransferase in flowers of Petunia hybrida

scientific article published on 01 March 1984

Quantification of serum apolipoproteins A-I and B-100 in clinical samples using an automated SISCAPA-MALDI-TOF-MS workflow

scientific article published on 9 March 2015

Quantifying protein measurands by peptide measurements: where do errors arise?

scientific article published on 6 January 2015

Regulatory Mechanisms of Calcineurin Phosphatase Activity ⬇️

scientific article published on January 1, 2011

Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory.

scientific article published on 29 January 2018

Spectrophotometric assay for calcineurin activity in leukocytes isolated from human blood.

scientific article published on 24 August 2006

Stimulation of cultured melanocytes in medium containing a serum substitute: Ultroser-G

scientific article published on 01 February 1995

Study of DT-diaphorase in pigment-producing cells

scientific article published on 01 November 1999

Subcellular fractionation of cultured normal human melanocytes: new insights into the relationship of melanosomes with lysosomes and peroxisomes.

scientific article published on March 1993

Temperature-dependent instability of the cTnI subunit in NIST SRM2921 characterized by tryptic peptide mapping.

scientific article

The combined effects of extracts containing carotenoids and vitamins E and C on growth and pigmentation of cultured human melanocytes.

scientific article published in September 2004

The effect of RBC transfusions on cytokine gene expression after cardiac surgery in patients developing post-operative multiple organ failure.

scientific article published on 24 April 2011

UVA1 radiation inhibits calcineurin through oxidative damage mediated by photosensitization

scientific article published on 24 February 2011

Ultraviolet-A (UVA-1) radiation suppresses immunoglobulin production of activated B lymphocytes in vitro

scientific article published on 01 September 2006

Variation in leukocyte subset concentrations affects calcineurin activity measurement: implications for pharmacodynamic monitoring strategies.

scientific article published on 24 January 2008

Variations in melanin formation by cultured melanocytes from different skin types

scientific article published on 01 June 1998

p16/cyclin-dependent kinase inhibitor 2A deficiency in human melanocyte senescence, apoptosis, and immortalization: possible implications for melanoma progression.

scientific article published on May 2003

List of works by Nico Smit

Automated Multiplex LC-MS/MS Assay for Quantifying Serum Apolipoproteins A-I, B, C-I, C-II, C-III, and E with Qualitative Apolipoprotein E Phenotyping

Biochemical characterization of a new melanoma model--the minipig MeLiM strain

Calcineurin activity and inhibition in skin and (epi)dermal cell cultures.

Catechol-O-methyltransferase in vitiligo

Coat color dilution in mice because of inactivation of the melanoma antigen MART-1

Cytotoxic interactions of Zn2+ in vitro: melanoma cells are more susceptible than melanocytes

Cytotoxicity of a selected series of substituted phenols towards cultured melanoma cells

Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands

Determination of catechol O-methyltransferase activity in relation to melanin metabolism using high-performance liquid chromatography with fluorimetric detection

Determination of pheomelanin by measurement of aminohydroxyphenylalanine isomers with high-performance liquid chromatography.

Disturbed melanin synthesis and chronic oxidative stress in dysplastic naevi

Effects of arsenite and UVA-1 radiation on calcineurin signaling.

Effects of ultraviolet A-1 radiation on calcineurin activity and cytokine production in (skin) cell cultures

Evaluation of interspecimen trypsin digestion efficiency prior to multiple reaction monitoring-based absolute protein quantification with native protein calibrators.

Everolimus and sirolimus antagonize tacrolimus based calcineurin inhibition via competition for FK-binding protein 12.

Evidence of the indirect formation of the catecholic intermediate substrate responsible for the autoactivation kinetics of tyrosinase.

Glutamate receptors on human melanocytes regulate the expression of MiTF.

Increased melanogenesis is a risk factor for oxidative DNA damage--study on cultured melanocytes and atypical nevus cells.

Keratinocytes control the pheo/eumelanin ratio in cultured normal human melanocytes

Large-scale cultivation of human melanocytes using collagen-coated Sephadex beads (cytodex 3)

Ligand-dependent activation of the melanocortin 5 receptor: cAMP production and ryanodine receptor-dependent elevations of [Ca(2+)](I).

Loss of adhesion to basement membrane components but not to keratinocytes in proliferating melanocytes

MS-based proteomics: a metrological sound and robust alternative for apolipoprotein E phenotyping in a multiplexed test

Melanin content of cultured human melanocytes and UV-induced cytotoxicity.

Melanin has a role in Ca2+ homeostasis in human melanocytes.

Melanogenesis in cultured melanocytes can be substantially influenced by L-tyrosine and L-cysteine

Melanogenesis in transfected fibroblasts induces lysosomal activation.

Melanosomal pH controls rate of melanogenesis, eumelanin/phaeomelanin ratio and melanosome maturation in melanocytes and melanoma cells.

Metabolic interference of melanogenesis in pigment cells.

Metrological traceability and harmonization of medical tests: a quantum leap forward is needed to keep pace with globalization and stringent IVD-regulations in the 21st century!

Metrological traceability in mass spectrometry-based targeted protein quantitation: a proof-of-principle study for serum apolipoproteins A-I and B100

Molecular diagnostics of calcineurin-related pathologies.

O-methylation of L-dopa in melanin metabolism and the presence of catechol-O-methyltransferase in melanocytes

Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers.

Properties and genetic control of anthocyanin 5-O-glucosyltransferase in flowers of Petunia hybrida

Quantification of serum apolipoproteins A-I and B-100 in clinical samples using an automated SISCAPA-MALDI-TOF-MS workflow

Quantifying protein measurands by peptide measurements: where do errors arise?

Regulatory Mechanisms of Calcineurin Phosphatase Activity ⬇️

Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory.

Spectrophotometric assay for calcineurin activity in leukocytes isolated from human blood.

Stimulation of cultured melanocytes in medium containing a serum substitute: Ultroser-G

Study of DT-diaphorase in pigment-producing cells

Subcellular fractionation of cultured normal human melanocytes: new insights into the relationship of melanosomes with lysosomes and peroxisomes.

Temperature-dependent instability of the cTnI subunit in NIST SRM2921 characterized by tryptic peptide mapping.

The combined effects of extracts containing carotenoids and vitamins E and C on growth and pigmentation of cultured human melanocytes.

The effect of RBC transfusions on cytokine gene expression after cardiac surgery in patients developing post-operative multiple organ failure.

UVA1 radiation inhibits calcineurin through oxidative damage mediated by photosensitization

Ultraviolet-A (UVA-1) radiation suppresses immunoglobulin production of activated B lymphocytes in vitro

Variation in leukocyte subset concentrations affects calcineurin activity measurement: implications for pharmacodynamic monitoring strategies.

Variations in melanin formation by cultured melanocytes from different skin types

p16/cyclin-dependent kinase inhibitor 2A deficiency in human melanocyte senescence, apoptosis, and immortalization: possible implications for melanoma progression.