List of works - Purnananda Guptasarma

'Super-perfect' enzymes: Structural stabilities and activities of recombinant triose phosphate isomerases from Pyrococcus furiosus and Thermococcus onnurineus produced in Escherichia coli.

scientific article published on 27 March 2015

A backbone-reversed form of an all-beta alpha-crystallin domain from a small heat-shock protein (retro-HSP12.6) folds and assembles into structured multimers.

scientific article published on 27 April 2003

Arsenic and 17-β-estradiol bind to each other and neutralize each other's signaling effects.

scientific article published on 23 June 2016

Attenuation of ionic interactions profoundly lowers the kinetic thermal stability of Pyrococcus furiosus triosephosphate isomerase.

scientific article published on 21 March 2009

Calcium binding to beta-2-microglobulin at physiological pH drives the occurrence of conformational changes which cause the protein to precipitate into amorphous forms that subsequently transform into amyloid aggregates.

scientific article

Characterization of a mildly alkalophilic and thermostable recombinant Thermus thermophilus laccase with applications in decolourization of dyes.

scientific article published on 23 October 2017

Coalescence of spherical beads of retro-HSP12.6 into linear and ring-shaped amyloid nanofibers.

scientific article published in June 2008

Confocal spectrofluorimetric evidence for the hetero-aggregation of sequence-scrambled forms of two model all-beta sheet proteins.

scientific article

Cooperative relaxation of supercoils and periodic transcriptional initiation within polymerase batteries.

scientific article

Creation of active TIM barrel enzymes through genetic fusion of half-barrel domain constructs derived from two distantly related glycosyl hydrolases

scientific article published on 17 October 2016

Design of a soluble mini-protein through tandem duplication of the minimally engineered beta hairpin 'tongue' motif of alpha-hemolysin.

scientific article

Dimorphic aggregation behavior of a fusion polypeptide incorporating a stable protein domain (EGFP) with an amyloidogenic sequence (retroCspA).

scientific article published on 20 May 2008

Direct N-terminal sequencing of polypeptides using a thermostable bacterial aminopeptidase and MALDI-TOF mass spectrometry.

scientific article published on 21 July 2015

Direct proteolysis-based purification of an overexpressed hyperthermophile protein from Escherichia coli lysate: a novel exploitation of the link between structural stability and proteolytic resistance.

scientific article

Dityrosine formation in the proteins of the eye lens

scientific article published on November 1, 1992

Endoglucanase activity at a second site in Pyrococcus furiosus triosephosphate isomerase-Promiscuity or compensation for a metabolic handicap?

scientific article published on 11 July 2017

Erratum to: Insufficient (Sub-native) Helix Content in Soluble/Solid Aggregates of Recombinant and Engineered Forms of IL-2 Throws Light on How Aggregated IL-2 is Biologically Active

scientific article published on 01 August 2015

Evidence of native-like substructure(s) in polypeptide chains of carbonic anhydrase deposited into insoluble aggregates during thermal unfolding

scientific article published on 01 January 2008

Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures.

scientific article published on 18 March 2008

Folding behavior of a backbone-reversed protein: reversible polyproline type II to beta-sheet thermal transitions in retro-GroES multimers with GroES-like features.

scientific article

Folding of beta/alpha-unit scrambled forms of S. cerevisiae triosephosphate isomerase: Evidence for autonomy of substructure formation and plasticity of hydrophobic and hydrogen bonding interactions in core of (beta/alpha)8-barrel.

scientific article

HU-AB simulacrum: Fusion of HU-B and HU-A into HU-B-A, a functional analog of the Escherichia coli HU-AB heterodimer

scientific article published in 2021

Hydrophobic dye inhibits aggregation of molten carbonic anhydrase during thermal unfolding and refolding

scientific article published on 01 November 1999

Hydroxyl radical mediated damage to proteins, with special reference to the crystallins

scientific article published on 01 May 1992

Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding

scientific article (publication date: 2014)

Identification and characterization of a spontaneously aggregating amyloid-forming variant of human PrP((90-231)) through phage-display screening of variants randomized between residues 101 and 112.

scientific article

Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening.

scientific article

Metal-catalyzed proteolysis, conformational antigenicity, photosensitized oxidation, and electrical dysfunction explain the pathogenicity of protein aggregates.

scientific article published on 08 April 2010

N-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of α-amino groups before tryptic digestion.

scientific article

Partial destabilization of native structure by a combination of heat and denaturant facilitates cold denaturation in a hyperthermophile protein.

scientific article published in August 2008

Peptide scanning-based identification of regions of gamma-II crystallin involved in thermal aggregation: evidence of the involvement of structurally analogous, helix-containing loops from the two double Greek key domains of the molecule

scientific article published on 01 February 2003

Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant.

scientific article published on 27 July 2015

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins.

scientific article published on 30 January 2018

Protein engineering of domains in flavoprotein disulphide oxidoreductases: contributions to folding and assembly.

scientific article published on February 1996

Reversal of peptide backbone direction may result in the mirroring of protein structure

scientific article published on October 5, 1992

Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system.

scientific article published on 14 June 2015

Slow irreversible unfolding of Pyrococcus furiosus triosephosphate isomerase: separation and quantitation of conformers through a novel electrophoretic approach.

scientific article published on 19 September 2005

Solution-state characteristics of the ultraviolet A-induced visible fluorescence from proteins.

scientific article

Structure-guided mutational evidence and postulates explaining how a glycohydrolase from Pyrococcus furiosus functions simultaneously as an amylase and as a 4-α-glucanotransferase

scientific article published on 11 January 2019

Structures of differently aggregated and precipitated forms of gamma B crystallin: an FTIR spectroscopic and EM study.

scientific article published in September 2010

The Achilles' Heel of "Ultrastable" Hyperthermophile Proteins: Submillimolar Concentrations of SDS Stimulate Rapid Conformational Change, Aggregation, and Amyloid Formation in Proteins Carrying Overall Positive Charge

scientific article published on 22 June 2016

The DNA-binding protein HU is a molecular glue that attaches bacteria to extracellular DNA in biofilms

scientific article published on 01 January 2021

The excised heat-shock domain of alphaB crystallin is a folded, proteolytically susceptible trimer with significant surface hydrophobicity and a tendency to self-aggregate upon heating.

scientific article published in August 2004

The key to the extraordinary thermal stability of P. furiosus holo-rubredoxin: iron binding-guided packing of a core aromatic cluster responsible for high kinetic stability of the native structure

scientific article

Understanding anomalous mobility of proteins on SDS-PAGE with special reference to the highly acidic extracellular domains of human E- and N-cadherins

scientific article published on 12 February 2019

Use of a hydrophobic dye to indirectly probe the structural organization and conformational plasticity of molecules in amorphous aggregates of carbonic anhydrase.

scientific article

Use of tandem cuvettes to determine whether radiative (trivial) energy transfer can contaminate steady-state measurements of fluorescence resonance energy transfer

scientific article published on 01 September 1995

List of works by Purnananda Guptasarma

'Super-perfect' enzymes: Structural stabilities and activities of recombinant triose phosphate isomerases from Pyrococcus furiosus and Thermococcus onnurineus produced in Escherichia coli.

A backbone-reversed form of an all-beta alpha-crystallin domain from a small heat-shock protein (retro-HSP12.6) folds and assembles into structured multimers.

Arsenic and 17-β-estradiol bind to each other and neutralize each other's signaling effects.

Attenuation of ionic interactions profoundly lowers the kinetic thermal stability of Pyrococcus furiosus triosephosphate isomerase.

Calcium binding to beta-2-microglobulin at physiological pH drives the occurrence of conformational changes which cause the protein to precipitate into amorphous forms that subsequently transform into amyloid aggregates.

Characterization of a mildly alkalophilic and thermostable recombinant Thermus thermophilus laccase with applications in decolourization of dyes.

Coalescence of spherical beads of retro-HSP12.6 into linear and ring-shaped amyloid nanofibers.

Confocal spectrofluorimetric evidence for the hetero-aggregation of sequence-scrambled forms of two model all-beta sheet proteins.

Cooperative relaxation of supercoils and periodic transcriptional initiation within polymerase batteries.

Creation of active TIM barrel enzymes through genetic fusion of half-barrel domain constructs derived from two distantly related glycosyl hydrolases

Design of a soluble mini-protein through tandem duplication of the minimally engineered beta hairpin 'tongue' motif of alpha-hemolysin.

Dimorphic aggregation behavior of a fusion polypeptide incorporating a stable protein domain (EGFP) with an amyloidogenic sequence (retroCspA).

Direct N-terminal sequencing of polypeptides using a thermostable bacterial aminopeptidase and MALDI-TOF mass spectrometry.

Direct proteolysis-based purification of an overexpressed hyperthermophile protein from Escherichia coli lysate: a novel exploitation of the link between structural stability and proteolytic resistance.

Dityrosine formation in the proteins of the eye lens

Endoglucanase activity at a second site in Pyrococcus furiosus triosephosphate isomerase-Promiscuity or compensation for a metabolic handicap?

Erratum to: Insufficient (Sub-native) Helix Content in Soluble/Solid Aggregates of Recombinant and Engineered Forms of IL-2 Throws Light on How Aggregated IL-2 is Biologically Active

Evidence of native-like substructure(s) in polypeptide chains of carbonic anhydrase deposited into insoluble aggregates during thermal unfolding

Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures.

Folding behavior of a backbone-reversed protein: reversible polyproline type II to beta-sheet thermal transitions in retro-GroES multimers with GroES-like features.

Folding of beta/alpha-unit scrambled forms of S. cerevisiae triosephosphate isomerase: Evidence for autonomy of substructure formation and plasticity of hydrophobic and hydrogen bonding interactions in core of (beta/alpha)8-barrel.

HU-AB simulacrum: Fusion of HU-B and HU-A into HU-B-A, a functional analog of the Escherichia coli HU-AB heterodimer

Hydrophobic dye inhibits aggregation of molten carbonic anhydrase during thermal unfolding and refolding

Hydroxyl radical mediated damage to proteins, with special reference to the crystallins

Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding

Identification and characterization of a spontaneously aggregating amyloid-forming variant of human PrP((90-231)) through phage-display screening of variants randomized between residues 101 and 112.

Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening.

Metal-catalyzed proteolysis, conformational antigenicity, photosensitized oxidation, and electrical dysfunction explain the pathogenicity of protein aggregates.

N-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of α-amino groups before tryptic digestion.

Partial destabilization of native structure by a combination of heat and denaturant facilitates cold denaturation in a hyperthermophile protein.

Peptide scanning-based identification of regions of gamma-II crystallin involved in thermal aggregation: evidence of the involvement of structurally analogous, helix-containing loops from the two double Greek key domains of the molecule

Probing protease sensitivity of recombinant human erythropoietin reveals α3-α4 inter-helical loop as a stability determinant.

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins.

Protein engineering of domains in flavoprotein disulphide oxidoreductases: contributions to folding and assembly.

Reversal of peptide backbone direction may result in the mirroring of protein structure

Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system.

Slow irreversible unfolding of Pyrococcus furiosus triosephosphate isomerase: separation and quantitation of conformers through a novel electrophoretic approach.

Solution-state characteristics of the ultraviolet A-induced visible fluorescence from proteins.

Structure-guided mutational evidence and postulates explaining how a glycohydrolase from Pyrococcus furiosus functions simultaneously as an amylase and as a 4-α-glucanotransferase

Structures of differently aggregated and precipitated forms of gamma B crystallin: an FTIR spectroscopic and EM study.

The Achilles' Heel of "Ultrastable" Hyperthermophile Proteins: Submillimolar Concentrations of SDS Stimulate Rapid Conformational Change, Aggregation, and Amyloid Formation in Proteins Carrying Overall Positive Charge

The DNA-binding protein HU is a molecular glue that attaches bacteria to extracellular DNA in biofilms

The excised heat-shock domain of alphaB crystallin is a folded, proteolytically susceptible trimer with significant surface hydrophobicity and a tendency to self-aggregate upon heating.

The key to the extraordinary thermal stability of P. furiosus holo-rubredoxin: iron binding-guided packing of a core aromatic cluster responsible for high kinetic stability of the native structure

Understanding anomalous mobility of proteins on SDS-PAGE with special reference to the highly acidic extracellular domains of human E- and N-cadherins

Use of a hydrophobic dye to indirectly probe the structural organization and conformational plasticity of molecules in amorphous aggregates of carbonic anhydrase.

Use of tandem cuvettes to determine whether radiative (trivial) energy transfer can contaminate steady-state measurements of fluorescence resonance energy transfer