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List of works by Robert T Sauer

1H-NMR study of the λ operator siteOL1: assignment of the imino and adenine H2 resonances ⬇️

scientific article published on May 11, 1984

A conserved activation cluster is required for allosteric communication in HtrA-family proteases.

scientific article published on 19 February 2015

A mutation in the N domain of Escherichia coli lon stabilizes dodecamers and selectively alters degradation of model substrates.

scientific article published on 11 October 2013

A simple fragment of cyclic acyldepsipeptides is necessary and sufficient for ClpP activation and antibacterial activity

scientific article published on 11 September 2014

AAA+ Proteases: ATP-Fueled Machines of Protein Destruction ⬇️

scientific article published on January 1, 2011

ATP-dependent proteases of bacteria: recognition logic and operating principles

scientific article published on 30 October 2006

Acceleration of the refolding of Arc repressor by nucleic acids and other polyanions

scientific article published on 01 June 1999

Allosteric activation of DegS, a stress sensor PDZ protease

scientific article

Allosteric regulation of DegS protease subunits through a shared energy landscape ⬇️

scientific article published on December 2, 2012

Allostery Is an Intrinsic Property of the Protease Domain of DegS: IMPLICATIONS FOR ENZYME FUNCTION AND EVOLUTION

scientific article

Altered specificity of a AAA+ protease.

scientific article published on January 2007

An evolutionary bridge to a new protein fold.

scientific article published in December 2000

Analyzing the interaction of RseA and RseB, the two negative regulators of the sigmaE envelope stress response, using a combined bioinformatic and experimental strategy

scientific article published on 22 December 2008

Antibacterial activity of and resistance to small molecule inhibitors of the ClpP peptidase

scientific article published on 4 October 2013

Architecture and assembly of the archaeal Cdc48*20S proteasome

scientific article

Are buried salt bridges important for protein stability and conformational specificity?

scientific article

Assaying the kinetics of protein denaturation catalyzed by AAA+ unfolding machines and proteases

scientific article published on 13 April 2015

Assignments of the 1H,13C, and 15N resonances of the substrate-binding SSD domain from Lon protease.

scientific article published in December 2001

Asymmetric interactions of ATP with the AAA+ ClpX6 unfoldase: allosteric control of a protein machine.

scientific article published in July 2005

Asymmetric nucleotide transactions of the HslUV protease.

scientific article published on 4 June 2008

Bacteriophage P22 Mnt repressor. DNA binding and effects on transcription in vitro.

scientific article published in May 1987

Barriers to protein folding: formation of buried polar interactions is a slow step in acquisition of structure.

scientific article published on April 1996

Biophysical characterization of the TraY protein of Escherichia coli F factor

scientific article published on 01 January 1998

Bipartite determinants mediate an evolutionarily conserved interaction between Cdc48 and the 20S peptidase

scientific article published on 11 February 2013

Bivalent tethering of SspB to ClpXP is required for efficient substrate delivery: a protein-design study.

scientific article published in February 2004

C-terminal domain mutations in ClpX uncouple substrate binding from an engagement step required for unfolding.

scientific article published in April 2003

Cage assembly of DegP protease is not required for substrate-dependent regulation of proteolytic activity or high-temperature cell survival

scientific article

Cleavage of the A site mRNA codon during ribosome pausing provides a mechanism for translational quality control

scientific article published on 01 October 2003

ClpAP proteolysis does not require rotation of the ClpA unfoldase relative to ClpP

scientific article published on 01 December 2020

ClpXP, an ATP-powered unfolding and protein-degradation machine ⬇️

scientific article published on June 27, 2011

Computational and experimental probes of symmetry mismatches in the Arc repressor-DNA complex.

scientific article

Control of Pseudomonas aeruginosa AlgW protease cleavage of MucA by peptide signals and MucB

scientific journal article

Control of phage P22 tail protein expression by transcription termination ⬇️

scientific article published on March 15, 1983

Control of substrate gating and translocation into ClpP by channel residues and ClpX binding.

scientific article

Coordinated gripping of substrate by subunits of a AAA+ proteolytic machine

scientific article published on 19 January 2015

Covalent Linkage of Distinct Substrate Degrons Controls Assembly and Disassembly of DegP Proteolytic Cages ⬇️

scientific article published on April 1, 2011

Covalent attachment of Arc repressor subunits by a peptide linker enhances affinity for operator DNA.

scientific article published in January 1996

Crystal structure of the nickel-responsive transcription factor NikR

scientific article published on 14 September 2003

Cytoplasmic degradation of ssrA-tagged proteins.

scientific article published in September 2005

Deciphering the Roles of Multicomponent Recognition Signals by the AAA+ Unfoldase ClpX.

scientific article

Degrons in protein substrates program the speed and operating efficiency of the AAA+ Lon proteolytic machine.

scientific article published on 19 October 2009

Deletion of the prc (tsp) gene provides evidence for additional tail-specific proteolytic activity in Escherichia coli K-12.

scientific article

Design, construction and characterization of a set of insulated bacterial promoters

scientific article

Direct and adaptor-mediated substrate recognition by an essential AAA+ protease

scholarly article

Dissection of Axial-Pore Loop Function during Unfolding and Translocation by a AAA+ Proteolytic Machine

scientific article published on 28 July 2015

Distinct peptide signals in the UmuD and UmuD' subunits of UmuD/D' mediate tethering and substrate processing by the ClpXP protease.

scientific article published on 31 October 2003

Distinct quaternary structures of the AAA+ Lon protease control substrate degradation.

scientific article

Distinct regulatory mechanisms balance DegP proteolysis to maintain cellular fitness during heat stress.

scientific article published on April 2014

Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP.

scientific article published on 24 February 2008

Diverse effects of mutations in the signal sequence on the secretion of beta-lactamase in Salmonella typhimurium.

scientific article published in October 1982

Diverse pore loops of the AAA+ ClpX machine mediate unassisted and adaptor-dependent recognition of ssrA-tagged substrates.

scientific article published in February 2008

Division of labor between the pore-1 loops of the D1 and D2 AAA+ rings coordinates substrate selectivity of the ClpAP protease

scientific article published on 12 November 2021

Domain structure and quaternary organization of the bacteriophage P22 Erf protein.

scientific article published on December 1983

Domains of Mnt repressor: roles in tetramer formation, protein stability, and operator DNA binding

scientific article published on 01 October 1995

Dual Molecular Signals Mediate the Bacterial Response to Outer-Membrane Stress ⬇️

scientific article published on May 17, 2013

Dynamic and static components power unfolding in topologically closed rings of a AAA+ proteolytic machine ⬇️

scientific article published on May 6, 2012

Energy-dependent degradation: Linkage between ClpX-catalyzed nucleotide hydrolysis and protein-substrate processing.

scientific article published in May 2003

Engineering controllable protein degradation.

scientific article published in June 2006

Engineering fluorescent protein substrates for the AAA+ Lon protease.

scientific article published on 28 January 2013

Engineering synthetic adaptors and substrates for controlled ClpXP degradation

scientific article published on 23 June 2009

Evidence for partial secondary structure formation in the transition state for arc repressor refolding and dimerization

scientific article published on 01 July 2000

Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease

scientific article

Examination of a Structural Model of Peptidomimicry by Cyclic Acyldepsipeptide Antibiotics in Their Interaction with the ClpP Peptidase

scientific article published on 06 July 2015

Expression of N-formylated proteins in Escherichia coli.

scientific article

Flexible linkers leash the substrate binding domain of SspB to a peptide module that stabilizes delivery complexes with the AAA+ ClpXP protease.

scientific article published in August 2003

Formation of a denatured dimer limits the thermal stability of Arc repressor 1 1Edited by P. E. Wright

article

Homology among DNA-binding proteins suggests use of a conserved super-secondary structure

scientific article published in Nature

Identification of active site residues of the Tsp protease.

scientific article published in December 1995

Identification of endogenous SsrA-tagged proteins reveals tagging at positions corresponding to stop codons.

scientific article

Identification of the Cdc48•20S proteasome as an ancient AAA+ proteolytic machine.

scientific article published on 26 July 2012

Inhibition of regulated proteolysis by RseB.

scholarly article

Interaction of mutant lambda repressors with operator and non-operator DNA

scientific article published on 01 November 1986

Interactions of Arg2 in the Mnt N-terminal arm with the central and flanking regions of the Mnt operator.

scientific article published on August 2000

Lambda repressor mutants that are better substrates for RecA-mediated cleavage.

scientific article

Lambda repressor mutations that increase the affinity and specificity of operator binding.

scientific article

Latent ClpX-recognition signals ensure LexA destruction after DNA damage.

scientific article

Linkage between ATP consumption and mechanical unfolding during the protein processing reactions of an AAA+ degradation machine.

scientific article published in August 2003

Mechanochemical basis of protein degradation by a double-ring AAA+ machine

scientific article

Modular and coordinated activity of AAA+ active sites in the double-ring ClpA unfoldase of the ClpAP protease

scientific article published on 05 October 2020

Molecular determinants of complex formation between Clp/Hsp100 ATPases and the ClpP peptidase.

scientific article

Multiple sequence signals direct recognition and degradation of protein substrates by the AAA+ protease HslUV.

scientific article published on 15 September 2010

Multistep substrate binding and engagement by the AAA+ ClpXP protease

scientific article published on 26 October 2020

Mutagenic dissection of the sequence determinants of protein folding, recognition, and machine function.

scientific article published on 18 September 2013

Mutational studies of protein stability and folding of the hyperstable MYL Arc repressor variant.

scientific article

NMR structure determination of the tetramerization domain of the Mnt repressor: An asymmetric alpha-helical assembly in slow exchange.

scientific article published in September 1999

Nickel coordination is regulated by the DNA-bound state of NikR.

scientific article published in February 2003

NikR repressor: high-affinity nickel binding to the C-terminal domain regulates binding to operator DNA.

scientific article published in October 2002

Nonlinear free energy relationships in Arc repressor unfolding imply the existence of unstable, native-like folding intermediates

scientific article published on 01 April 1996

Nucleotide Binding and Conformational Switching in the Hexameric Ring of a AAA+ Machine

scientific article

Nucleotide-dependent substrate handoff from the SspB adaptor to the AAA+ ClpXP protease.

scientific article published in November 2004

Nucleotide-dependent substrate recognition by the AAA+ HslUV protease.

scientific article

OMP Peptides Activate the DegS Stress-Sensor Protease by a Relief of Inhibition Mechanism

scientific article

OMP peptides modulate the activity of DegS protease by differential binding to active and inactive conformations.

scientific article published on January 2009

Optimizing the stability of single-chain proteins by linker length and composition mutagenesis ⬇️

scientific article published on May 26, 1998

Overexpression of CupB5 activates alginate overproduction in Pseudomonas aeruginosa by a novel AlgW-dependent mechanism

scientific article

Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis.

scientific article

P22 Arc repressor: role of cooperativity in repression and binding to operators with altered half-site spacing

scientific article published on 01 June 1995

PDZ-like domains mediate binding specificity in the Clp/Hsp100 family of chaperones and protease regulatory subunits

scientific article published on 01 December 1997

Polypeptide translocation by the AAA+ ClpXP protease machine.

scientific article published on June 2009

Preferential heterodimer formation via undercompensated electrostatic interactions.

scientific article published in February 2001

Primary structure of the immI immunity region of bacteriophage P22 ⬇️

scientific article published on August 25, 1983

Protein stabilization by removal of unsatisfied polar groups: computational approaches and experimental tests

scientific article published on 01 June 1996

Protein unfolding and degradation by the AAA+ Lon protease ⬇️

scientific article published on January 4, 2012

Protein unfolding by a AAA+ protease is dependent on ATP-hydrolysis rates and substrate energy landscapes.

scientific article published on 27 January 2008

Proteomic Profiling of ClpXP Substrates after DNA Damage Reveals Extensive Instability within SOS Regulon

article

Regulatory functions of the lambda repressor reside in the amino-terminal domain.

scientific article published in May 1979

Remodeling of a delivery complex allows ClpS-mediated degradation of N-degron substrates.

scientific article

Restriction of the conformational dynamics of the cyclic acyldepsipeptide antibiotics improves their antibacterial activity

scientific article published on 24 January 2014

Reverse hydrophobic effects relieved by amino-acid substitutions at a protein surface.

scientific article published in March 1990

Revisiting the mechanism of macrolide-antibiotic resistance mediated by ribosomal protein L22.

scholarly article

Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules

scholarly article

Role of an N(cap) residue in determining the stability and operator-binding affinity of Arc repressor.

scientific article published in January 2003

Roles of the N domain of the AAA+ Lon protease in substrate recognition, allosteric regulation and chaperone activity.

scientific article

Sculpting the proteome with AAA(+) proteases and disassembly machines.

scientific article published in October 2004

Sequence determinants of C-terminal substrate recognition by the Tsp protease.

scientific article

Sequence space, folding and protein design

scientific article

Signal detection by the PhoQ sensor-transmitter. Characterization of the sensor domain and a response-impaired mutant that identifies ligand-binding determinants

scientific article published on 01 October 1996

Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA.

scientific article published in November 2001

Single amino acid substitutions uncouple the DNA binding and strand scission activities of Fok I endonuclease.

scientific article published on October 1993

Single-molecule protein unfolding and translocation by an ATP-fueled proteolytic machine.

scientific article

Small-molecule control of protein degradation using split adaptors.

scientific article published on 8 September 2011

Specificity of minor-groove and major-groove interactions in a homeodomain-DNA complex.

scientific article

Specificity versus stability in computational protein design

scientific article

Stability and DNA binding of the phd protein of the phage P1 plasmid addiction system.

scientific article published in January 1999

Stepwise unfolding of a β barrel protein by the AAA+ ClpXP protease.

scientific article

Stochastic but highly coordinated protein unfolding and translocation by the ClpXP proteolytic machine.

scientific article published on July 2014

Striking stabilization of Arc repressor by an engineered disulfide bond

scientific article published on 01 October 2000

Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates

scientific article published on 22 October 2020

Structural biology. A glimpse into tmRNA-mediated ribosome rescue.

scientific article

Structure and dynamics of the tetrameric mnt repressor and a model for its DNA complex.

scientific article

Structure of a Delivery Protein for an AAA+ Protease in Complex with a Peptide Degradation Tag ⬇️

scientific article published on August 1, 2003

Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate

scientific article published on 28 February 2020

Substrate delivery by the AAA+ ClpX and ClpC1 unfoldases activates the mycobacterial ClpP1P2 peptidase

scientific article published on 13 July 2014

Subunit asymmetry and roles of conformational switching in the hexameric AAA+ ring of ClpX

scientific article published on 13 April 2015

The AAA+ ClpX machine unfolds a keystone subunit to remodel the Mu transpososome.

scientific article

The ClpS Adaptor Mediates Staged Delivery of N-End Rule Substrates to the AAA+ ClpAP Protease

scientific article

The Doc toxin and Phd antidote proteins of the bacteriophage P1 plasmid addiction system form a heterotrimeric complex.

scientific article published in June 1999

The I domain of the AAA+ HslUV protease coordinates substrate binding, ATP hydrolysis, and protein degradation

scientific article published on 4 January 2012

The tetramerization domain of the Mnt repressor consists of two right-handed coiled coils

scientific article

The tmRNA system for translational surveillance and ribosome rescue.

scientific article published on January 2007

Toxin-antitoxin pairs in bacteria: killers or stress regulators?

scientific article published on 01 January 2003

Versatile modes of peptide recognition by the AAA+ adaptor protein SspB.

scientific article

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