List of works - Elizabeth M. Gillam

'S-SXR-RMs'? Selective SXR response modulators--the future of designer drug interactions?

scientific article published on 01 August 2002

A shuffled CYP1A library shows both structural integrity and functional diversity.

scientific article

A shuffled CYP2C library with a high degree of structural integrity and functional versatility.

scientific article

An evaluation of potential mechanism-based inactivation of human drug metabolizing cytochromes P450 by monoamine oxidase inhibitors, including isoniazid.

scientific article published in May 2006

Assessment of arginine 97 and lysine 72 as determinants of substrate specificity in cytochrome P450 2C9 (CYP2C9).

scientific article published in April 2004

Association of CYP1B1 genetic polymorphism with incidence to breast and lung cancer

scientific article published on 01 February 2000

Bacterial expression of two human aryl sulfotransferases

scientific article published on 01 February 1998

Bioactivation of phenytoin by human cytochrome P450: characterization of the mechanism and targets of covalent adduct formation.

scientific article published on September 1997

Cellular energy charge in the heart and liver of the rat. The effects of ethanol and acetaldehyde

scientific article published on 01 January 1986

Characterization of the human cytochrome P450 forms involved in metabolism of tamoxifen to its alpha-hydroxy and alpha,4-dihydroxy derivatives.

scientific article published in October 2005

Computational tools for directed evolution: a comparison of prospective and retrospective strategies.

scientific article

Cytochrome P450-mediated metabolism of haloperidol and reduced haloperidol to pyridinium metabolites.

scientific article

Differential expression of cytochrome P450 enzymes from the CYP2C subfamily in the human brain.

scientific article published on 11 December 2014

Differential expression of human cytochrome P450 enzymes from the CYP3A subfamily in the brains of alcoholic subjects and drug-free controls.

scientific article published on 14 March 2013

Directed evolution of cytochrome P450 enzymes for biocatalysis: exploiting the catalytic versatility of enzymes with relaxed substrate specificity.

scientific article published on April 2015

Directed evolution reveals requisite sequence elements in the functional expression of P450 2F1 in Escherichia coli

scientific article

Drug metabolism by Escherichia coli expressing human cytochromes P450 ⬇️

scientific article published on August 1, 1997

Echinacea metabolism and drug interactions: the case for standardization of a complementary medicine.

scientific article published on 08 May 2009

Emerging roles for brain drug-metabolizing cytochrome P450 enzymes in neuropsychiatric conditions and responses to drugs.

scientific article

Engineering thermostable CYP2D enzymes for biocatalysis using combinatorial libraries of ancestors for directed evolution (CLADE)

Exploring the past and the future of protein evolution with ancestral sequence reconstruction: the 'retro' approach to protein engineering.

scientific article published on January 2017

Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.

scientific article

Expression of CYP2E1 and CYP2U1 proteins in amygdala and prefrontal cortex: influence of alcoholism and smoking

scientific article

Expression of Cytochrome-P450-3A5 in Escherichia Coli: Effects of 5′ Modification, Purification, Spectral Characterization, Reconstitution Conditions, and Catalytic Activities

scientific article published on 01 March 1995

Expression of cytochrome P450 2D6 in Escherichia coli, purification, and spectral and catalytic characterization

scientific article published on 01 June 1995

Expression of cytochrome P450 3A7 in Escherichia coli: effects of 5' modification and catalytic characterization of recombinant enzyme expressed in bicistronic format with NADPH-cytochrome P450 reductase

scientific article published on 01 October 1997

Expression of human cytochrome P450 enzymes in yeast and bacteria and relevance to studies on catalytic specificity.

scientific article

Expression of modified human cytochrome P450 1A1 in Escherichia coli: effects of 5' substitution, stabilization, purification, spectral characterization, and catalytic properties

scientific article published on 01 August 1994

Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties.

scientific article

Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme.

scientific article

Extending the capabilities of nature's most versatile catalysts: directed evolution of mammalian xenobiotic-metabolizing P450s.

scientific article published on 15 May 2007

Extending the diversity of cytochrome P450 enzymes by DNA family shuffling.

scientific article

Formation of a dihydroxy metabolite of phenytoin in human liver microsomes/cytosol: roles of cytochromes P450 2C9, 2C19, and 3A4

scientific article published on 01 November 2000

Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

scientific article

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR.

scientific article published on 22 June 2015

Human cytochrome P450 enzymes expressed in bacteria: reagents to probe molecular interactions in toxicology

scientific article published on 01 November 1998

Identification of the human cytochromes P450 catalysing the rate-limiting pathways of gliclazide elimination

scientific article

Identification, Synthesis, and Biological Evaluation of the Major Human Metabolite of NLRP3 Inflammasome Inhibitor MCC950.

scientific article published on 27 September 2016

Immunotoxic side-effects of drug therapy.

scientific article

In silico characterization of protein chimeras: relating sequence and function within the same fold.

scientific article

In vitro metabolism of the anti-inflammatory clerodane diterpenoid polyandric acid A and its hydrolysis product by human liver microsomes and recombinant cytochrome P450 and UDP-glucuronosyltransferase enzymes

scientific article published on 14 July 2016

Membrane integration of recombinant human P450 forms.

scientific article

Metabolism of benzo[a]pyrene to trans-7,8-dihydroxy-7, 8-dihydrobenzo[a]pyrene by recombinant human cytochrome P450 1B1 and purified liver epoxide hydrolase.

scientific article published in July 1999

Metabolism of tamoxifen by recombinant human cytochrome P450 enzymes: formation of the 4-hydroxy, 4'-hydroxy and N-desmethyl metabolites and isomerization of trans-4-hydroxytamoxifen.

scientific article

Metabolism of the major Echinacea alkylamide N-isobutyldodeca-2E,4E,8Z,10Z-tetraenamide by human recombinant cytochrome P450 enzymes and human liver microsomes.

scientific article published in August 2010

Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.

scientific article published in April 2005

Molecular modelling of human CYP1B1 substrate interactions and investigation of allelic variant effects on metabolism.

scientific article published in June 2003

New applications of bacterial systems to problems in toxicology.

scientific article published on September 1996

P450 2C18 catalyzes the metabolic bioactivation of phenytoin.

scientific article published in December 2005

Phenytoin metabolism by human cytochrome P450: involvement of P450 3A and 2C forms in secondary metabolism and drug-protein adduct formation.

scientific article published in August 2000

Physical partitioning as the major source of metoprolol uptake by hepatic microsomes

scientific article published on 01 December 1987

Prospects for Applying Synthetic Biology to Toxicology: Future Opportunities and Current Limitations for the Repurposing of Cytochrome P450 Systems.

scientific article published on 13 December 2016

Rabbit CYP4B1 engineered for high-level expression in Escherichia coli: ligand stabilization and processing of the N-terminus and heme prosthetic group.

scientific article published on August 2003

Re-engineering of CYP2C9 to probe acid-base substrate selectivity

scientific article

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries.

scientific article

Recombinant expression and characterization of Lucilia cuprina CYP6G3: Activity and binding properties toward multiple pesticides.

scientific article

Recombinant human cytochrome P450 1B1 expression in Escherichia coli

scientific article published on 01 September 1998

Reconstitution of recombinant cytochrome P450 2C10(2C9) and comparison with cytochrome P450 3A4 and other forms: effects of cytochrome P450-P450 and cytochrome P450-b5 interactions.

scientific article

Requirements for cytochrome b5 in the oxidation of 7-ethoxycoumarin, chlorzoxazone, aniline, and N-nitrosodimethylamine by recombinant cytochrome P450 2E1 and by human liver microsomes

scientific article published on 01 July 1996

Restriction enzyme-mediated DNA family shuffling

scientific article

Roles of NADPH-P450 reductase in the O-deethylation of 7-ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli.

scientific article

STAR: predicting recombination sites from amino acid sequence

scientific article

Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: purification, activity, and binding properties toward multiple pesticides.

scientific article published on 5 March 2013

Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability

scientific article published on 10 March 2020

The PXR ligand-binding domain: how to be picky and promiscuous at the same time.

scientific article

The concept lens diagram: a new mechanism for presenting biochemistry content in terms of "big ideas"

scientific article published on 01 July 2011

The dark side of a 'detoxification' mechanism.

scientific article

The evolution of cytochrome P450 enzymes as biocatalysts in drug discovery and development.

scientific article

Transgenic xenosensors: humanizing mice

scientific article published on 01 September 2000

Use of heterologously-expressed cytochrome P450 and glutathione transferase enzymes in toxicity assays.

scientific article

List of works by Elizabeth M. Gillam

'S-SXR-RMs'? Selective SXR response modulators--the future of designer drug interactions?

A shuffled CYP1A library shows both structural integrity and functional diversity.

A shuffled CYP2C library with a high degree of structural integrity and functional versatility.

An evaluation of potential mechanism-based inactivation of human drug metabolizing cytochromes P450 by monoamine oxidase inhibitors, including isoniazid.

Assessment of arginine 97 and lysine 72 as determinants of substrate specificity in cytochrome P450 2C9 (CYP2C9).

Association of CYP1B1 genetic polymorphism with incidence to breast and lung cancer

Bacterial expression of two human aryl sulfotransferases

Bioactivation of phenytoin by human cytochrome P450: characterization of the mechanism and targets of covalent adduct formation.

Cellular energy charge in the heart and liver of the rat. The effects of ethanol and acetaldehyde

Characterization of the human cytochrome P450 forms involved in metabolism of tamoxifen to its alpha-hydroxy and alpha,4-dihydroxy derivatives.

Computational tools for directed evolution: a comparison of prospective and retrospective strategies.

Cytochrome P450-mediated metabolism of haloperidol and reduced haloperidol to pyridinium metabolites.

Differential expression of cytochrome P450 enzymes from the CYP2C subfamily in the human brain.

Differential expression of human cytochrome P450 enzymes from the CYP3A subfamily in the brains of alcoholic subjects and drug-free controls.

Directed evolution of cytochrome P450 enzymes for biocatalysis: exploiting the catalytic versatility of enzymes with relaxed substrate specificity.

Directed evolution reveals requisite sequence elements in the functional expression of P450 2F1 in Escherichia coli

Drug metabolism by Escherichia coli expressing human cytochromes P450 ⬇️

Echinacea metabolism and drug interactions: the case for standardization of a complementary medicine.

Emerging roles for brain drug-metabolizing cytochrome P450 enzymes in neuropsychiatric conditions and responses to drugs.

Engineering thermostable CYP2D enzymes for biocatalysis using combinatorial libraries of ancestors for directed evolution (CLADE)

Exploring the past and the future of protein evolution with ancestral sequence reconstruction: the 'retro' approach to protein engineering.

Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.

Expression of CYP2E1 and CYP2U1 proteins in amygdala and prefrontal cortex: influence of alcoholism and smoking

Expression of Cytochrome-P450-3A5 in Escherichia Coli: Effects of 5′ Modification, Purification, Spectral Characterization, Reconstitution Conditions, and Catalytic Activities

Expression of cytochrome P450 2D6 in Escherichia coli, purification, and spectral and catalytic characterization

Expression of cytochrome P450 3A7 in Escherichia coli: effects of 5' modification and catalytic characterization of recombinant enzyme expressed in bicistronic format with NADPH-cytochrome P450 reductase

Expression of human cytochrome P450 enzymes in yeast and bacteria and relevance to studies on catalytic specificity.

Expression of modified human cytochrome P450 1A1 in Escherichia coli: effects of 5' substitution, stabilization, purification, spectral characterization, and catalytic properties

Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties.

Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme.

Extending the capabilities of nature's most versatile catalysts: directed evolution of mammalian xenobiotic-metabolizing P450s.

Extending the diversity of cytochrome P450 enzymes by DNA family shuffling.

Formation of a dihydroxy metabolite of phenytoin in human liver microsomes/cytosol: roles of cytochromes P450 2C9, 2C19, and 3A4

Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

Gene expression profiling of cytochromes P450, ABC transporters and their principal transcription factors in the amygdala and prefrontal cortex of alcoholics, smokers and drug-free controls by qRT-PCR.

Human cytochrome P450 enzymes expressed in bacteria: reagents to probe molecular interactions in toxicology

Identification of the human cytochromes P450 catalysing the rate-limiting pathways of gliclazide elimination

Identification, Synthesis, and Biological Evaluation of the Major Human Metabolite of NLRP3 Inflammasome Inhibitor MCC950.

Immunotoxic side-effects of drug therapy.

In silico characterization of protein chimeras: relating sequence and function within the same fold.

In vitro metabolism of the anti-inflammatory clerodane diterpenoid polyandric acid A and its hydrolysis product by human liver microsomes and recombinant cytochrome P450 and UDP-glucuronosyltransferase enzymes

Membrane integration of recombinant human P450 forms.

Metabolism of benzo[a]pyrene to trans-7,8-dihydroxy-7, 8-dihydrobenzo[a]pyrene by recombinant human cytochrome P450 1B1 and purified liver epoxide hydrolase.

Metabolism of tamoxifen by recombinant human cytochrome P450 enzymes: formation of the 4-hydroxy, 4'-hydroxy and N-desmethyl metabolites and isomerization of trans-4-hydroxytamoxifen.

Metabolism of the major Echinacea alkylamide N-isobutyldodeca-2E,4E,8Z,10Z-tetraenamide by human recombinant cytochrome P450 enzymes and human liver microsomes.

Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.

Molecular modelling of human CYP1B1 substrate interactions and investigation of allelic variant effects on metabolism.

New applications of bacterial systems to problems in toxicology.

P450 2C18 catalyzes the metabolic bioactivation of phenytoin.

Phenytoin metabolism by human cytochrome P450: involvement of P450 3A and 2C forms in secondary metabolism and drug-protein adduct formation.

Physical partitioning as the major source of metoprolol uptake by hepatic microsomes

Prospects for Applying Synthetic Biology to Toxicology: Future Opportunities and Current Limitations for the Repurposing of Cytochrome P450 Systems.

Rabbit CYP4B1 engineered for high-level expression in Escherichia coli: ligand stabilization and processing of the N-terminus and heme prosthetic group.

Re-engineering of CYP2C9 to probe acid-base substrate selectivity

ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries.

Recombinant expression and characterization of Lucilia cuprina CYP6G3: Activity and binding properties toward multiple pesticides.

Recombinant human cytochrome P450 1B1 expression in Escherichia coli

Reconstitution of recombinant cytochrome P450 2C10(2C9) and comparison with cytochrome P450 3A4 and other forms: effects of cytochrome P450-P450 and cytochrome P450-b5 interactions.

Requirements for cytochrome b5 in the oxidation of 7-ethoxycoumarin, chlorzoxazone, aniline, and N-nitrosodimethylamine by recombinant cytochrome P450 2E1 and by human liver microsomes

Restriction enzyme-mediated DNA family shuffling

Roles of NADPH-P450 reductase in the O-deethylation of 7-ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli.

STAR: predicting recombination sites from amino acid sequence

Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: purification, activity, and binding properties toward multiple pesticides.

Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability

The PXR ligand-binding domain: how to be picky and promiscuous at the same time.

The concept lens diagram: a new mechanism for presenting biochemistry content in terms of "big ideas"

The dark side of a 'detoxification' mechanism.

The evolution of cytochrome P450 enzymes as biocatalysts in drug discovery and development.

Transgenic xenosensors: humanizing mice

Use of heterologously-expressed cytochrome P450 and glutathione transferase enzymes in toxicity assays.