List of works - Marc Ostermeier

A Comprehensive, High-Resolution Map of a Gene's Fitness Landscape.

scientific article

A Positive Selection for Nucleoside Kinases in E. coli

scientific article

A comprehensive, high-resolution map of a gene's fitness landscape

scientific article

A hot-spot motif characterizes the interface between a designed ankyrin-repeat protein and its target ligand.

scientific article published on 7 February 2012

A molecular switch created by in vitro recombination of nonhomologous genes.

scientific article published in November 2004

A protein therapeutic modality founded on molecular regulation.

scientific article

A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination

scientific article

Advancements in the development of HIF-1α-activated protein switches for use in enzyme prodrug therapy

scientific article

An engineered split M.HhaI-zinc finger fusion lacks the intended methyltransferase specificity.

scientific article published on October 2008

An externally tunable bacterial band-pass filter.

scholarly article

Beyond cataloging the Library of Babel.

scientific article

Characterization of monobody scaffold interactions with ligand via force spectroscopy and steered molecular dynamics.

scientific article published on 4 February 2015

Circular permutation in the Ω-loop of TEM-1 β-lactamase results in improved activity and altered substrate specificity

scientific article

Collateral fitness effects of mutations

scientific article published on 08 May 2020

Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation

scientific article published on 30 April 2019

Construction of Protein Switches by Domain Insertion and Directed Evolution.

scientific article

Creation of an allosteric enzyme by domain insertion.

scientific article

Design and synthesis of a beta-lactamase activated 5-fluorouracil prodrug.

scientific article

Design of protein switches based on an ensemble model of allostery

scientific article

Development of a cancer-marker activated enzymatic switch from the herpes simplex virus thymidine kinase

scientific article

Directed evolution of improved zinc finger methyltransferases

scientific article

Directed evolution of protein switches and their application to the creation of ligand-binding proteins.

scientific article published in August 2005

Electrochemical activation of engineered protein switches

scientific article

Environmental changes bridge evolutionary valleys

scientific article

Enzymatic protein switches built from paralogous input domains

scientific article

Fitness Effects of Single Amino Acid Insertions and Deletions in TEM-1 β-Lactamase

scientific article published on 26 April 2019

Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells.

scientific article

Hybrid enzymes: manipulating enzyme design ⬇️

scientific article published on June 1, 1998

In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells

scientific article (publication date: 2011)

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

scientific article published on 15 August 2015

Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression

scientific article

Mathematical expressions useful in the construction, description and evaluation of protein libraries.

scientific article published in June 2005

Modulation of effector affinity by hinge region mutations also modulates switching activity in an engineered allosteric TEM1 beta-lactamase switch.

scientific article

NMR characterization of an engineered domain fusion between maltose binding protein and TEM1 beta-lactamase provides insight into its structure and allosteric mechanism.

scientific article published in May 2010

PFunkel: efficient, expansive, user-defined mutagenesis.

scientific article

Pervasive Pairwise Intragenic Epistasis among Sequential Mutations in TEM-1 β-Lactamase

scientific article published on 25 March 2019

Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria

scientific article published in PLoS ONE

Protein switch engineering by domain insertion.

scientific article

Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled‐form plasmid DNA ⬇️

scientific article published on June 15, 2011

Protein-Programmed Accumulation of Yeast Cytosine Deaminase in Cancer Cells in Response to Mock-Hypoxia

scientific article published on 23 April 2019

Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

scientific article

Rational design of a fusion protein to exhibit disulfide-mediated logic gate behavior.

scientific article published on 27 August 2014

Shifting Fitness and Epistatic Landscapes Reflect Trade-offs along an Evolutionary Pathway

scientific article

Sites in the AAV5 capsid tolerant to deletions and tandem duplications

scientific article

Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation ⬇️

scientific article published on June 14, 2012

Structure prediction of domain insertion proteins from structures of individual domains.

scientific article

Surface-tethered protein switches

scientific article published on 18 February 2011

Synthetic gene libraries: in search of the optimal diversity.

scientific article

Targeted DNA methylation in human cells using engineered dCas9-methyltransferases.

scientific article

The high-affinity maltose switch MBP317-347 has low affinity for glucose: implications for targeting tumors with metabolically directed enzyme prodrug therapy

scientific article published on 26 December 2013

The interplay between effector binding and allostery in an engineered protein switch.

scientific article

The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus confer 3'-azido-3'-deoxythymidine resistance to Escherichia coli.

scientific article published on 5 November 2014

Theoretical distribution of truncation lengths in incremental truncation libraries.

scientific article

Thermostability Enhancement of GH 62 α- l -Arabinofuranosidase by Directed Evolution and Rational Design

scientific article published on 14 February 2024

List of works by Marc Ostermeier

A Comprehensive, High-Resolution Map of a Gene's Fitness Landscape.

A Positive Selection for Nucleoside Kinases in E. coli

A comprehensive, high-resolution map of a gene's fitness landscape

A hot-spot motif characterizes the interface between a designed ankyrin-repeat protein and its target ligand.

A molecular switch created by in vitro recombination of nonhomologous genes.

A protein therapeutic modality founded on molecular regulation.

A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination

Advancements in the development of HIF-1α-activated protein switches for use in enzyme prodrug therapy

An engineered split M.HhaI-zinc finger fusion lacks the intended methyltransferase specificity.

An externally tunable bacterial band-pass filter.

Beyond cataloging the Library of Babel.

Characterization of monobody scaffold interactions with ligand via force spectroscopy and steered molecular dynamics.

Circular permutation in the Ω-loop of TEM-1 β-lactamase results in improved activity and altered substrate specificity

Collateral fitness effects of mutations

Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation

Construction of Protein Switches by Domain Insertion and Directed Evolution.

Creation of an allosteric enzyme by domain insertion.

Design and synthesis of a beta-lactamase activated 5-fluorouracil prodrug.

Design of protein switches based on an ensemble model of allostery

Development of a cancer-marker activated enzymatic switch from the herpes simplex virus thymidine kinase

Directed evolution of improved zinc finger methyltransferases

Directed evolution of protein switches and their application to the creation of ligand-binding proteins.

Electrochemical activation of engineered protein switches

Environmental changes bridge evolutionary valleys

Enzymatic protein switches built from paralogous input domains

Fitness Effects of Single Amino Acid Insertions and Deletions in TEM-1 β-Lactamase

Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells.

Hybrid enzymes: manipulating enzyme design ⬇️

In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression

Mathematical expressions useful in the construction, description and evaluation of protein libraries.

Modulation of effector affinity by hinge region mutations also modulates switching activity in an engineered allosteric TEM1 beta-lactamase switch.

NMR characterization of an engineered domain fusion between maltose binding protein and TEM1 beta-lactamase provides insight into its structure and allosteric mechanism.

PFunkel: efficient, expansive, user-defined mutagenesis.

Pervasive Pairwise Intragenic Epistasis among Sequential Mutations in TEM-1 β-Lactamase

Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria

Protein switch engineering by domain insertion.

Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled‐form plasmid DNA ⬇️

Protein-Programmed Accumulation of Yeast Cytosine Deaminase in Cancer Cells in Response to Mock-Hypoxia

Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

Rational design of a fusion protein to exhibit disulfide-mediated logic gate behavior.

Shifting Fitness and Epistatic Landscapes Reflect Trade-offs along an Evolutionary Pathway

Sites in the AAV5 capsid tolerant to deletions and tandem duplications

Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation ⬇️

Structure prediction of domain insertion proteins from structures of individual domains.

Surface-tethered protein switches

Synthetic gene libraries: in search of the optimal diversity.

Targeted DNA methylation in human cells using engineered dCas9-methyltransferases.

The high-affinity maltose switch MBP317-347 has low affinity for glucose: implications for targeting tumors with metabolically directed enzyme prodrug therapy

The interplay between effector binding and allostery in an engineered protein switch.

The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus confer 3'-azido-3'-deoxythymidine resistance to Escherichia coli.

Theoretical distribution of truncation lengths in incremental truncation libraries.

Thermostability Enhancement of GH 62 α- l -Arabinofuranosidase by Directed Evolution and Rational Design