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List of works by Piotr Skowron

A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

scientific article published on July 2003

A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

scientific article

A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognising 5′-TARCCA(N11/9)-3′ sequences

scientific article published on 04 December 2014

A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.

scientific article published in March 2016

A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications

scientific article published on 13 November 2019

An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

scientific article published on 24 June 2020

Antimicrobial Potential of the Genera Geobacillus and Parageobacillus, as Well as Endolysins Biosynthesized by Their Bacteriophages

scientific article published in 2022

Artificial plasmid labeled with 5-bromo-2'-deoxyuridine: a universal molecular system for strand break detection

scientific article published on 21 May 2014

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

scientific article

Boosting toxic protein biosynthesis: transient in vivo inactivation of engineered bacterial alkaline phosphatase

scientific article published on 18 August 2020

Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency

scientific article published on 01 June 2011

Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family

scientific article published on 29 May 2009

Cloning and applications of the two/three-base restriction endonuclease R·CviJI from IL-3A virus-infected chlorella ⬇️

scientific article published on May 19, 1995

Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N₄₇₃A variant in the NPPY motif

scientific article published on 19 January 2014

Correction: Sequence, genome organization, annotation and proteomics of the thermophilic, 47.7-kb Geobacillus stearothermophilus bacteriophage TP-84 and its classification in the new Tp84virus genus.

scientific article published on 26 April 2018

Crystallization and preliminary X-ray diffraction analysis of Val57 mutants of the amyloidogenic protein human cystatin C.

scientific article published on 26 November 2011

Data regarding a new, vector-enzymatic DNA fragment amplification-expression technology for the construction of artificial, concatemeric DNA, RNA and proteins, as well as biological effects of selected polypeptides obtained using this method

scientific article published on 31 December 2019

Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.

scientific article published on 17 February 2016

Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence

scientific article

Epigenetic inhibitor zebularine activates ear pinna wound closure in the mouse

scientific article published on 11 July 2019

Identification of bacterial species in probiotic consortiums in selected commercial cleaning preparations

scientific article published on 01 June 2019

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells

scientific article published on 27 July 2018

Imunofan-RDKVYR Peptide-Stimulates Skin Cell Proliferation and Promotes Tissue Repair

scientific article published on 23 June 2020

MmeI, a class-IIS restriction endonuclease: Purification and characterization ⬇️

scientific article published on May 19, 1995

MmoSTI restriction endonuclease, isolated from Morganella morganii infecting a tropical moth, Actias selene, cleaving 5'-|CCNGG-3' sequences

scientific article published on 18 August 2015

Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

scientific article published on 11 January 2014

NMR and crystallographic structural studies of the extremely stable monomeric variant of human cystatin C with single amino acid substitution.

scientific article

Novel parameter describing restriction endonucleases: Secondary-Cognate-Specificity and chemical stimulation of TsoI leading to substrate specificity change

scientific article published on 16 March 2019

PCR synthesis of double stranded DNA labeled with 5-bromouridine. A step towards finding a bromonucleoside for clinical trials.

scientific article

Purification and characterization of the restriction endonuclease AvcI from actinomyces cristalomycini ⬇️

scientific article published on May 19, 1995

Randomized DNA libraries construction tool: a new 3-bp 'frequent cutter' TthHB27I/sinefungin endonuclease with chemically-induced specificity.

scientific article

Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities

scientific article published on 10 April 2012

Sequence, genome organization, annotation and proteomics of the thermophilic, 47.7-kb Geobacillus stearothermophilus bacteriophage TP-84 and its classification in the new Tp84virus genus.

scientific article published on 6 April 2018

Structural characterization of V57D and V57P mutants of human cystatin C, an amyloidogenic protein

scientific article

The promises and risks of probiotic Bacillus species

scientific article published on 01 December 2018

The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.

scientific article published on September 2017

Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene

scientific article published on 17 October 2017

Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

scientific article

TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.

scientific article published on April 2002

Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

scientific article published on 6 December 2014

Unexpected photoproduct generated via the acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine in a water/isopropanol solution: experimental and computational studies

scientific article published on 30 November 2010

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