List of works - Ashley M Toye

A new junctional hierarchy.

scientific article

An N-terminal GFP tag does not alter the functional expression to the plasma membrane of red cell and kidney anion exchanger (AE1) in mammalian cells.

scientific article published in July 2002

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

scientific article published on 14 March 2017

Band 3 Courcouronnes (Ser667Phe): a trafficking mutant differentially rescued by wild-type band 3 and glycophorin A.

scientific article published on 03 January 2008

Band 3 Walton, a C-terminal deletion associated with distal renal tubular acidosis, is expressed in the red cell membrane but retained internally in kidney cells.

scientific article

Band 3 function and dysfunction in a structural context.

scientific article published on 12 February 2018

CD47 surface stability is sensitive to actin disruption prior to inclusion within the band 3 macrocomplex

scientific article

CFTR potentiators partially restore channel function to A561E-CFTR, a cystic fibrosis mutant with a similar mechanism of dysfunction as F508del-CFTR

scientific article

Characteristic phenotypes associated with congenital dyserythropoietic anemia (type II) manifest at different stages of erythropoiesis

scientific article published on 09 August 2013

Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation.

scientific article published on 22 March 2016

Critical band 3 multiprotein complex interactions establish early during human erythropoiesis.

scientific article published on 28 April 2011

Defective kidney anion-exchanger 1 (AE1, Band 3) trafficking in dominant distal renal tubular acidosis (dRTA).

scientific article

Differential Proteomic Analysis of Human Erythroblasts Undergoing Apoptosis Induced by Epo-Withdrawal ⬇️

scientific article published on June 18, 2012

Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing

scientific article published on 26 April 2018

Ephrin/Eph receptor interaction facilitates macrophage recognition of differentiating human erythroblasts

scientific article published on 13 June 2019

Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

scientific article published on 15 March 2023

Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

scientific article published on 23 August 2019

Glucocorticoids induce differentiation of monocytes towards macrophages that share functional and phenotypical aspects with erythroblastic island macrophages

scientific article published on 28 December 2017

Glyceraldehyde 3-phosphate dehydrogenase is required for band 3 (anion exchanger 1) membrane residency in the mammalian kidney

scientific journal article

Glycophorin A: Band 3 aid.

scientific article published on 04 March 2008

HCO(3)(-)-independent conductance with a mutant Na(+)/HCO(3)(-) cotransporter (SLC4A4) in a case of proximal renal tubular acidosis with hypokalaemic paralysis

scientific article

Immunohistochemical comparison of a case of inherited distal renal tubular acidosis (with a unique AE1 mutation) with an acquired case secondary to autoimmune disease

scientific article published on 05 January 2007

Investigating the key membrane protein changes during in vitro erythropoiesis of protein 4.2 (-) cells (mutations Chartres 1 and 2).

scientific article

Keratinocyte Superoxide Generation ⬇️

scientific article published on February 1, 1998

Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression.

scientific article published on 4 June 2018

Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte

scientific article

Non-muscle Myosin II drives vesicle loss during human reticulocyte maturation

scientific article published on 03 August 2018

Opsin stability and folding: the role of Cys185 and abnormal disulfide bond formation in the intradiscal domain.

scientific article published on 10 October 2007

Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors.

scientific article published on 30 August 2016

Protein distribution during human erythroblast enucleation in vitro

scientific article

Protein-4.2 association with band 3 (AE1, SLCA4) in Xenopus oocytes: effects of three natural protein-4.2 mutations associated with hemolytic anemia.

scientific article published on 3 February 2005

Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS

scientific article

Quantitative measurement of red cell surface protein expression reveals new biomarkers for hereditary spherocytosis

scientific article published on 10 May 2018

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

scientific article published on 01 January 2018

Rapid diagnosis of hereditary haemolytic anaemias using automated rheoscopy and supervised machine learning

scientific article published on 05 July 2020

Red-cell glycophorin A-band 3 interactions associated with the movement of band 3 to the cell surface.

scientific article published on August 2000

Regions of human kidney anion exchanger 1 (kAE1) required for basolateral targeting of kAE1 in polarised kidney cells: mis-targeting explains dominant renal tubular acidosis (dRTA).

scientific article published on 2 March 2004

Relative CO₂/NH₃ permeabilities of human RhAG, RhBG and RhCG.

scientific article published in December 2013

Remodelling of the erythrocyte cytoskeleton during P. falciparum merozoite invasion

scientific article published on 22 September 2014

Reticulocyte and red blood cell deformation triggers specific phosphorylation events

scientific article published on 01 September 2019

RhCG is the major putative ammonia transporter expressed in the human kidney, and RhBG is not expressed at detectable levels.

scientific article published on 08 April 2009

Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts

scientific article published on 31 May 2016

The human NBCe1-A mutant R881C, associated with proximal renal tubular acidosis, retains function but is mistargeted in polarized renal epithelia

scientific article published on 17 May 2006

The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.

scientific article published on 7 April 2010

The sorting of blood group active proteins during enucleation

scientific article

Transduction with BBF2H7/CREB3L2 upregulates SEC23A protein in erythroblasts and partially corrects the hypo-glycosylation phenotype associated with CDAII

scientific article published on 14 March 2018

Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation.

scientific article published on 29 April 2019

Young infants exhibit robust functional antibody responses and restrained IFN-γ production to SARS-CoV-2

publication published on 09 June 2021

List of works by Ashley M Toye

A new junctional hierarchy.

An N-terminal GFP tag does not alter the functional expression to the plasma membrane of red cell and kidney anion exchanger (AE1) in mammalian cells.

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

Band 3 Courcouronnes (Ser667Phe): a trafficking mutant differentially rescued by wild-type band 3 and glycophorin A.

Band 3 Walton, a C-terminal deletion associated with distal renal tubular acidosis, is expressed in the red cell membrane but retained internally in kidney cells.

Band 3 function and dysfunction in a structural context.

CD47 surface stability is sensitive to actin disruption prior to inclusion within the band 3 macrocomplex

CFTR potentiators partially restore channel function to A561E-CFTR, a cystic fibrosis mutant with a similar mechanism of dysfunction as F508del-CFTR

Characteristic phenotypes associated with congenital dyserythropoietic anemia (type II) manifest at different stages of erythropoiesis

Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation.

Critical band 3 multiprotein complex interactions establish early during human erythropoiesis.

Defective kidney anion-exchanger 1 (AE1, Band 3) trafficking in dominant distal renal tubular acidosis (dRTA).

Differential Proteomic Analysis of Human Erythroblasts Undergoing Apoptosis Induced by Epo-Withdrawal ⬇️

Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing

Ephrin/Eph receptor interaction facilitates macrophage recognition of differentiating human erythroblasts

Evaluation and deployment of isotype-specific salivary antibody assays for detecting previous SARS-CoV-2 infection in children and adults

Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

Glucocorticoids induce differentiation of monocytes towards macrophages that share functional and phenotypical aspects with erythroblastic island macrophages

Glyceraldehyde 3-phosphate dehydrogenase is required for band 3 (anion exchanger 1) membrane residency in the mammalian kidney

Glycophorin A: Band 3 aid.

HCO(3)(-)-independent conductance with a mutant Na(+)/HCO(3)(-) cotransporter (SLC4A4) in a case of proximal renal tubular acidosis with hypokalaemic paralysis

Immunohistochemical comparison of a case of inherited distal renal tubular acidosis (with a unique AE1 mutation) with an acquired case secondary to autoimmune disease

Investigating the key membrane protein changes during in vitro erythropoiesis of protein 4.2 (-) cells (mutations Chartres 1 and 2).

Keratinocyte Superoxide Generation ⬇️

Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression.

Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte

Non-muscle Myosin II drives vesicle loss during human reticulocyte maturation

Opsin stability and folding: the role of Cys185 and abnormal disulfide bond formation in the intradiscal domain.

Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors.

Protein distribution during human erythroblast enucleation in vitro

Protein-4.2 association with band 3 (AE1, SLCA4) in Xenopus oocytes: effects of three natural protein-4.2 mutations associated with hemolytic anemia.

Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS

Quantitative measurement of red cell surface protein expression reveals new biomarkers for hereditary spherocytosis

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

Rapid diagnosis of hereditary haemolytic anaemias using automated rheoscopy and supervised machine learning

Red-cell glycophorin A-band 3 interactions associated with the movement of band 3 to the cell surface.

Regions of human kidney anion exchanger 1 (kAE1) required for basolateral targeting of kAE1 in polarised kidney cells: mis-targeting explains dominant renal tubular acidosis (dRTA).

Relative CO₂/NH₃ permeabilities of human RhAG, RhBG and RhCG.

Remodelling of the erythrocyte cytoskeleton during P. falciparum merozoite invasion

Reticulocyte and red blood cell deformation triggers specific phosphorylation events

RhCG is the major putative ammonia transporter expressed in the human kidney, and RhBG is not expressed at detectable levels.

Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts

The human NBCe1-A mutant R881C, associated with proximal renal tubular acidosis, retains function but is mistargeted in polarized renal epithelia

The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.

The sorting of blood group active proteins during enucleation

Transduction with BBF2H7/CREB3L2 upregulates SEC23A protein in erythroblasts and partially corrects the hypo-glycosylation phenotype associated with CDAII

Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation.

Young infants exhibit robust functional antibody responses and restrained IFN-γ production to SARS-CoV-2