List of works - Gema Alvarez-García

A century of bovine besnoitiosis: an unknown disease re-emerging in Europe.

scientific article published on 03 July 2013

A model for chronic bovine besnoitiosis: Parasite stage and inoculation route are key factors

scientific article published on 18 September 2019

A new lyophilized tachyzoite based ELISA to diagnose Besnoitia spp. infection in bovids and wild ruminants improves specificity.

scientific article published on 25 July 2017

A serosurvey of selected cystogenic coccidia in Spanish equids: first detection of anti-Besnoitia spp. specific antibodies in Europe.

scientific article

A time-resolved fluorescence immunoassay for the detection of anti-Neospora caninum antibodies in sheep

scientific article published on 19 November 2019

A vaccine formulation combining rhoptry proteins NcROP40 and NcROP2 improves pup survival in a pregnant mouse model of neosporosis.

scientific article

Abortions in bovines and Neospora caninum transmission in an embryo transfer center

scientific article published on 26 June 2010

Absence of antibodies specific to Besnoitia spp. in European sheep and goats from areas in Spain where bovine besnoitiosis is endemic.

scientific article published on 4 November 2016

Added value of IgM detection and low avidity index as markers of acute bovine besnoitiosis

scientific article published on 13 December 2019

Advances in the diagnosis of bovine besnoitiosis: current options and applications for control.

scientific article published on 8 September 2017

An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle.

scientific article published on 16 September 2017

An inter-laboratory comparative study of serological tools employed in the diagnosis of Besnoitia besnoiti infection in bovines.

scientific article published on 19 March 2012

Anti-Neospora caninum and anti-Sarcocystis spp. specific antibodies cross-react with Besnoitia besnoiti and influence the serological diagnosis of bovine besnoitiosis.

scientific article published on 8 September 2015

Besnoitia besnoiti among cattle in insular and northwestern Italy: endemic infection or isolated outbreaks?

scientific article

Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates.

scientific article published on 29 February 2016

Bovine chronic besnoitiosis in a calf: Characterization of a novel B. besnoiti isolate from an unusual case report.

scientific article published on 23 September 2017

Characterization of an outbreak of emerging bovine besnoitiosis in southwestern Spain.

scientific article published on 14 April 2016

Characterization of pathology and parasite load in outbred and inbred mouse models of chronic Neospora caninum infection.

scientific article

Characterization of the Neospora caninum NcROP40 and NcROP2Fam-1 rhoptry proteins during the tachyzoite lytic cycle.

scientific article published on 2 November 2015

Chronic bovine besnoitiosis: intra-organ parasite distribution, parasite loads and parasite-associated lesions in subclinical cases.

scientific article

Clinical and Serological Dynamics of Besnoitia besnoiti Infection in Three Endemically Infected Beef Cattle Herds.

scientific article published on 24 August 2015

Detection of Toxoplasma gondii antibodies in Antarctic pinnipeds.

scientific article

Development and characterization of monoclonal antibodies against Besnoitia besnoiti tachyzoites

scientific article published on 16 August 2018

Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain

scientific article published on 01 June 2010

Dynamics of Besnoitia besnoiti infection in cattle

scientific article

EFFECT OF DIFFERENT ECOSYSTEMS AND MANAGEMENT PRACTICES ON TOXOPLASMA GONDII AND NEOSPORA CANINUM INFECTIONS IN WILD RUMINANTS IN SPAIN.

scientific article

Effect of parasite dose and host age on the infection with Besnoitia besnoiti tachyzoites in cattle

scientific article published on 12 August 2018

Exposure to Neospora spp. and Besnoitia spp. in wildlife from Israel

scholarly article published by Daniel Gutiérrez-Expósito in 2018

Failure of a vaccine using immunogenic recombinant proteins rNcSAG4 and rNcGRA7 against neosporosis in mice.

scientific article published on 25 September 2009

First 2-DE approach towards characterising the proteome and immunome of Besnoitia besnoiti in the tachyzoite stage.

scientific article

First detection of anti-Besnoitia spp. specific antibodies in horses and donkeys in Italy

scientific article published on 20 June 2018

First isolation of Besnoitia besnoiti from a chronically infected cow in Spain.

scientific article

First report of Neospora caninum infection in adult alpacas (Vicugna pacos) and llamas (Lama glama)

scientific article published on 01 August 2004

From the mainland to Ireland - bovine besnoitiosis and its spread in Europe.

scientific article published on June 2016

Genetic characterization of Neospora caninum from aborted bovine foetuses in Aguascalientes, Mexico

scientific article published on 11 September 2016

Hypodermosis of red deer in Spain

scientific article published on 01 April 2001

Identification and molecular cloning of the Neospora caninum SAG4 gene specifically expressed at bradyzoite stage.

scientific article

Identification of Neospora caninum proteins regulated during the differentiation process from tachyzoite to bradyzoite stage by DIGE.

scientific article published in May 2010

Identification of a gene cluster for cell-surface genes of the SRS superfamily in Neospora caninum and characterization of the novel SRS9 gene.

scientific article published on 23 September 2011

Identification of novel rhoptry proteins in Neospora caninum by LC/MS-MS analysis of subcellular fractions.

scientific article published on 21 February 2011

In vitro efficacy of bumped kinase inhibitors against Besnoitia besnoiti tachyzoites.

scientific article published on 9 September 2017

In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum ⬇️

scientific article

Infected dendritic cells facilitate systemic dissemination and transplacental passage of the obligate intracellular parasite Neospora caninum in mice

scientific article

Influence of age and purpose for testing on the cut-off selection of serological methods in bovine neosporosis

scientific article published on 01 May 2003

Isolation and characterization of a bovine isolate of Neospora caninum with low virulence.

scientific article published on 11 October 2008

Low rates of Neospora caninum infection reactivation during gestation are observed in both chronically and congenitally infected mice.

scientific article published on 12 October 2012

Molecular characterisation of BSR4, a novel bradyzoite-specific gene from Neospora caninum.

scientific article published on 17 February 2007

Neospora caninum infection as a cause of reproductive failure in a sheep flock

scientific article

Neospora caninum infection in breeder bulls: seroprevalence and comparison of serological methods used for diagnosis

scientific article published on 01 September 2004

Neospora caninum seroprevalence in dairy and beef cattle from the northwest region of Spain, Galicia.

scientific article published on 9 December 2010

Neospora caninum tachyzoite immunome study reveals differences among three biologically different isolates

scientific article published on 21 August 2015

Neospora caninum tachyzoites inoculated by the conjunctival route are not vertically transmitted in pregnant cattle: a descriptive study

scientific article published on 16 October 2013

Nodular onchocercosis of red deer in central Spain

scientific article published on 01 May 2003

Pattern of recognition of Besnoitia besnoiti tachyzoite and bradyzoite antigens by naturally infected cattle

scientific article published on 24 June 2009

Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses

scientific article published on 01 July 2002

Peripheral and placental immune responses in goats after primoinfection with Neospora caninum at early, mid and late gestation.

scientific article published on 24 May 2017

Prevalence of Besnoitia besnoiti infection in beef cattle from the Spanish Pyrenees

scientific article published on 25 February 2014

Proteome expression changes among virulent and attenuated Neospora caninum isolates

scientific article published on 10 February 2012

Proteomics reveals differences in protein abundance and highly similar antigenic profiles between Besnoitia besnoiti and Besnoitia tarandi.

scientific article

Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR

scientific article

Repurposing of commercially available anti-coccidials identifies diclazuril and decoquinate as potential therapeutic candidates against Besnoitia besnoiti infection

scientific article published on 31 August 2018

Serological diagnosis of bovine neosporosis: a comparative study of commercially available ELISA tests

scientific article published on 01 August 2013

Serological dynamics and risk factors of Besnoitia besnoiti infection in breeding bulls from an endemically infected purebred beef herd

scientific article published on 10 March 2017

Seroprevalence and risk factors associated with Neospora caninum infection in different dog populations in Spain

scientific article published on 08 December 2007

Seroprevalence of Besnoitia besnoiti infection and associated risk factors in cattle from an endemic region in Europe.

scientific article published on 25 February 2014

Seroprevalence of Leptospirosis, Brucellosis, and Q Fever in a Wild Red Deer (Cervus elaphus) Population Kept in a Fenced Reserve in Absence of Contact with Livestock

scientific article published on 5 September 2017

Specific antibody responses against Neospora caninum recombinant rNcGRA7, rNcSAG4, rNcBSR4 and rNcSRS9 proteins are correlated with virulence in mice.

scientific article

Stage-specific expression of Nc SAG4 as a marker of chronic Neospora caninum infection in a mouse model.

scientific article published on 30 April 2009

Systemic Besnoitiosis in a Juvenile Roe Deer (Capreolus capreolus).

scientific article published on 26 May 2016

Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice

scientific article

The NcGRA7 gene encodes the immunodominant 17 kDa antigen of Neospora caninum.

scientific article published on 11 October 2006

The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation

scientific article published on 03 May 2012

The role of wild ruminants as reservoirs of Besnoitia besnoiti infection in cattle.

scientific article published on 5 April 2016

The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress.

scientific article published on 21 June 2016

Toxoplasma gondii and Neospora caninum seroprevalences in domestic South American camelids of the Peruvian Andes

scientific article published on 25 May 2014

Toxoplasma gondii infection in adult llamas (Lama glama) and vicunas (Vicugnavicugna) in the Peruvian Andean region

scientific article published on 26 April 2005

Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice

article

Use of an immunodominant P17 antigenic fraction of Neospora caninum in detection of antibody response in cattle

scientific article published on 01 August 2006

Use of avidity enzyme-linked immunosorbent assay and avidity Western blot to discriminate between acute and chronic Neospora caninum infection in cattle.

scientific article published in September 2005

Usefulness of rNcGRA7- and rNcSAG4-based ELISA tests for distinguishing primo-infection, recrudescence, and chronic bovine neosporosis.

scientific article published on 13 August 2008

List of works by Gema Alvarez-García

A century of bovine besnoitiosis: an unknown disease re-emerging in Europe.

A model for chronic bovine besnoitiosis: Parasite stage and inoculation route are key factors

A new lyophilized tachyzoite based ELISA to diagnose Besnoitia spp. infection in bovids and wild ruminants improves specificity.

A serosurvey of selected cystogenic coccidia in Spanish equids: first detection of anti-Besnoitia spp. specific antibodies in Europe.

A time-resolved fluorescence immunoassay for the detection of anti-Neospora caninum antibodies in sheep

A vaccine formulation combining rhoptry proteins NcROP40 and NcROP2 improves pup survival in a pregnant mouse model of neosporosis.

Abortions in bovines and Neospora caninum transmission in an embryo transfer center

Absence of antibodies specific to Besnoitia spp. in European sheep and goats from areas in Spain where bovine besnoitiosis is endemic.

Added value of IgM detection and low avidity index as markers of acute bovine besnoitiosis

Advances in the diagnosis of bovine besnoitiosis: current options and applications for control.

An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle.

An inter-laboratory comparative study of serological tools employed in the diagnosis of Besnoitia besnoiti infection in bovines.

Anti-Neospora caninum and anti-Sarcocystis spp. specific antibodies cross-react with Besnoitia besnoiti and influence the serological diagnosis of bovine besnoitiosis.

Besnoitia besnoiti among cattle in insular and northwestern Italy: endemic infection or isolated outbreaks?

Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates.

Bovine chronic besnoitiosis in a calf: Characterization of a novel B. besnoiti isolate from an unusual case report.

Characterization of an outbreak of emerging bovine besnoitiosis in southwestern Spain.

Characterization of pathology and parasite load in outbred and inbred mouse models of chronic Neospora caninum infection.

Characterization of the Neospora caninum NcROP40 and NcROP2Fam-1 rhoptry proteins during the tachyzoite lytic cycle.

Chronic bovine besnoitiosis: intra-organ parasite distribution, parasite loads and parasite-associated lesions in subclinical cases.

Clinical and Serological Dynamics of Besnoitia besnoiti Infection in Three Endemically Infected Beef Cattle Herds.

Detection of Toxoplasma gondii antibodies in Antarctic pinnipeds.

Development and characterization of monoclonal antibodies against Besnoitia besnoiti tachyzoites

Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain

Dynamics of Besnoitia besnoiti infection in cattle

EFFECT OF DIFFERENT ECOSYSTEMS AND MANAGEMENT PRACTICES ON TOXOPLASMA GONDII AND NEOSPORA CANINUM INFECTIONS IN WILD RUMINANTS IN SPAIN.

Effect of parasite dose and host age on the infection with Besnoitia besnoiti tachyzoites in cattle

Exposure to Neospora spp. and Besnoitia spp. in wildlife from Israel

Failure of a vaccine using immunogenic recombinant proteins rNcSAG4 and rNcGRA7 against neosporosis in mice.

First 2-DE approach towards characterising the proteome and immunome of Besnoitia besnoiti in the tachyzoite stage.

First detection of anti-Besnoitia spp. specific antibodies in horses and donkeys in Italy

First isolation of Besnoitia besnoiti from a chronically infected cow in Spain.

First report of Neospora caninum infection in adult alpacas (Vicugna pacos) and llamas (Lama glama)

From the mainland to Ireland - bovine besnoitiosis and its spread in Europe.

Genetic characterization of Neospora caninum from aborted bovine foetuses in Aguascalientes, Mexico

Hypodermosis of red deer in Spain

Identification and molecular cloning of the Neospora caninum SAG4 gene specifically expressed at bradyzoite stage.

Identification of Neospora caninum proteins regulated during the differentiation process from tachyzoite to bradyzoite stage by DIGE.

Identification of a gene cluster for cell-surface genes of the SRS superfamily in Neospora caninum and characterization of the novel SRS9 gene.

Identification of novel rhoptry proteins in Neospora caninum by LC/MS-MS analysis of subcellular fractions.

In vitro efficacy of bumped kinase inhibitors against Besnoitia besnoiti tachyzoites.

In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum ⬇️

Infected dendritic cells facilitate systemic dissemination and transplacental passage of the obligate intracellular parasite Neospora caninum in mice

Influence of age and purpose for testing on the cut-off selection of serological methods in bovine neosporosis

Isolation and characterization of a bovine isolate of Neospora caninum with low virulence.

Low rates of Neospora caninum infection reactivation during gestation are observed in both chronically and congenitally infected mice.

Molecular characterisation of BSR4, a novel bradyzoite-specific gene from Neospora caninum.

Neospora caninum infection as a cause of reproductive failure in a sheep flock

Neospora caninum infection in breeder bulls: seroprevalence and comparison of serological methods used for diagnosis

Neospora caninum seroprevalence in dairy and beef cattle from the northwest region of Spain, Galicia.

Neospora caninum tachyzoite immunome study reveals differences among three biologically different isolates

Neospora caninum tachyzoites inoculated by the conjunctival route are not vertically transmitted in pregnant cattle: a descriptive study

Nodular onchocercosis of red deer in central Spain

Pattern of recognition of Besnoitia besnoiti tachyzoite and bradyzoite antigens by naturally infected cattle

Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses

Peripheral and placental immune responses in goats after primoinfection with Neospora caninum at early, mid and late gestation.

Prevalence of Besnoitia besnoiti infection in beef cattle from the Spanish Pyrenees

Proteome expression changes among virulent and attenuated Neospora caninum isolates

Proteomics reveals differences in protein abundance and highly similar antigenic profiles between Besnoitia besnoiti and Besnoitia tarandi.

Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR

Repurposing of commercially available anti-coccidials identifies diclazuril and decoquinate as potential therapeutic candidates against Besnoitia besnoiti infection

Serological diagnosis of bovine neosporosis: a comparative study of commercially available ELISA tests

Serological dynamics and risk factors of Besnoitia besnoiti infection in breeding bulls from an endemically infected purebred beef herd

Seroprevalence and risk factors associated with Neospora caninum infection in different dog populations in Spain

Seroprevalence of Besnoitia besnoiti infection and associated risk factors in cattle from an endemic region in Europe.

Seroprevalence of Leptospirosis, Brucellosis, and Q Fever in a Wild Red Deer (Cervus elaphus) Population Kept in a Fenced Reserve in Absence of Contact with Livestock

Specific antibody responses against Neospora caninum recombinant rNcGRA7, rNcSAG4, rNcBSR4 and rNcSRS9 proteins are correlated with virulence in mice.

Stage-specific expression of Nc SAG4 as a marker of chronic Neospora caninum infection in a mouse model.

Systemic Besnoitiosis in a Juvenile Roe Deer (Capreolus capreolus).

Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice

The NcGRA7 gene encodes the immunodominant 17 kDa antigen of Neospora caninum.

The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation

The role of wild ruminants as reservoirs of Besnoitia besnoiti infection in cattle.

The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress.

Toxoplasma gondii and Neospora caninum seroprevalences in domestic South American camelids of the Peruvian Andes

Toxoplasma gondii infection in adult llamas (Lama glama) and vicunas (Vicugnavicugna) in the Peruvian Andean region

Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice

Use of an immunodominant P17 antigenic fraction of Neospora caninum in detection of antibody response in cattle

Use of avidity enzyme-linked immunosorbent assay and avidity Western blot to discriminate between acute and chronic Neospora caninum infection in cattle.