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List of works by Hiroshi Iwasaki

A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast

scientific article

A yeast gene, MGS1, encoding a DNA-dependent AAA(+) ATPase is required to maintain genome stability.

scientific article

An in vitro assay for monitoring the formation and branch migration of holliday junctions mediated by a eukaryotic recombinase

scientific article published on 01 January 2011

Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions.

scientific article

Aphidicolin inhibits DNA polymerizing activity but not nucleolytic activity of Escherichia coli DNA polymerase II

scientific article published on 01 February 1992

Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli

scientific article

Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.

scientific article published on 30 May 2011

Cleavage of symmetric immobile DNA junctions by Escherichia coli RuvC

scientific article published on 01 October 2000

Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres

scientific article

Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex

scientific article published on 24 March 2020

Crystal structure of the Holliday junction DNA in complex with a single RuvA tetramer

scientific article published in July 2000

Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery

scientific article

DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres

scientific article published on 30 April 2020

Direct evidence that a conserved arginine in RuvB AAA+ ATPase acts as an allosteric effector for the ATPase activity of the adjacent subunit in a hexamer.

scholarly article

Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition

scientific article

Establishment of DNA-DNA Interactions by the Cohesin Ring

scientific article published on 10 January 2018

Evidence that phenylalanine 69 in Escherichia coli RuvC resolvase forms a stacking interaction during binding and destabilization of a Holliday junction DNA substrate.

scientific article

Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast

scientific article published on 31 August 2010

Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.

scientific article published on 15 February 2007

Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.

scientific article

Functional overlap between RecA and MgsA (RarA) in the rescue of stalled replication forks in Escherichia coli

scientific article published on 01 March 2005

Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis

scientific article

Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair.

scientific article published on 25 May 2005

Holliday junction resolvases mediate chloroplast nucleoid segregation

scientific article published in May 2017

Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase.

scientific article

In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum.

scientific article published on 30 May 2017

Involvement in DNA repair of the ruvA gene of Escherichia coli.

scientific article

Mating-type switching by homology-directed recombinational repair: a matter of choice

scientific article published on 31 October 2018

Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates.

scientific article published in December 1996

Molecular characterization of the Schizosaccharomyces pombe nbs1+ gene involved in DNA repair and telomere maintenance

scientific article published in September 2003

Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex.

scientific article published on 31 March 2008

Molecular mechanisms of Holliday junction processing in Escherichia coli.

scientific article published on January 1995

Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.

scientific article

Mutational analysis of the functional motifs of RuvB, an AAA+ class helicase and motor protein for holliday junction branch migration

scientific article published on 01 May 2000

Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC

scientific article published on 01 September 1998

Nbs1 Flexibly Tethers Ctp1 and Mre11-Rad50 to Coordinate DNA Double-Strand Break Processing and Repair

scientific article

New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.

scientific article

No braiding of Holliday junctions in positively supercoiled DNA molecules

scientific article published on 01 December 1999

Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme.

scientific article

Parallel symmetric immobile DNA junctions as substrates for E. coli RuvC Holliday junction resolvase

scientific article published on 01 September 2002

Proteolytic processing of MucA protein in SOS mutagenesis: both processed and unprocessed MucA may be active in the mutagenesis.

scientific article published in November 1990

RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light.

scientific article

Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast

scientific article

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

scientific article published on 13 February 2019

Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51

scientific article published on 11 June 2020

RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.

scientific article

RecA requires two molecules of Mg2+ ions for its optimal strand exchange activity in vitro

scientific article published on 30 January 2018

Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators

scientific article

Recruitment and delivery of the fission yeast Rst2 transcription factor via a local genome structure counteracts repression by Tup1-family corepressors.

scientific article published in September 2017

Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.

scientific article published on August 2004

Role of the RuvAB protein in avoiding spontaneous formation of deletion mutations in the Escherichia coli K-12 endogenous tonB gene

article

Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates

scientific article

Role of walker motif A of RuvB protein in promoting branch migration of holliday junctions. Walker motif a mutations affect Atp binding, Atp hydrolyzing, and DNA binding activities of Ruvb

scientific article published on 01 September 1999

Roles of the recG gene product of Escherichia coli in recombination repair: effects of the delta recG mutation on cell division and chromosome partition.

scientific article

SOS-inducible DNA polymerase II of E coli is homologous to replicative DNA polymerase of eukaryotes.

scientific article published on April 1991

SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA ⬇️

scientific article published on October 1, 1991

Srs2 plays a critical role in reversible G2 arrest upon chronic and low doses of UV irradiation via two distinct homologous recombination-dependent mechanisms in postreplication repair-deficient cells.

scientific article published on 16 August 2010

Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.

scientific article

Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament.

scientific article published on 03 December 2013

Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation

article published in the Proceedings of the National Academy of Sciences of the United States of America

The Gene Is Essential for Repairing Double-Strand DNA Breaks Spontaneously Occurring during Replication and Induced by DNA-Damaging Agents

The 4th International Symposium on 3R; DNA replication, recombination and repair

scientific article published on 01 February 2004

The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents.

scientific article

The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.

scientific article

The differentiated and conserved roles of Swi5-Sfr1 in homologous recombination.

scientific article

The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51.

scientific article

Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms

scientific article published on 15 May 2020

Two basic residues, Lys-107 and Lys-118, of RuvC resolvase are involved in critical contacts with the Holliday junction for its resolution

scientific article published on 01 October 2000

Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast

scientific article published on 8 December 2003

Two different oligomeric states of the RuvB branch migration motor protein as revealed by electron microscopy

scientific article published on 01 August 2000

Two three-strand intermediates are processed during Rad51-driven DNA strand exchange

scientific article published on 4 December 2017

Uncoupling of the ATPase activity from the branch migration activity of RuvAB protein complexes containing both wild-type and ATPase-defective RuvB proteins

scientific article published on 01 September 2003

p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC.

scientific article

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