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List of works by Hiroaki FUNAHASHI

A microfluidic device to reduce treatment time of intracytoplasmic sperm injection ⬇️

scientific article published on November 2, 2012

A phosphodiesterase type-5 inhibitor, sildenafil, induces sperm capacitation and penetration into porcine oocytes in a chemically defined medium.

scientific article published on 12 September 2015

Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos.

scientific article published on 13 December 2015

Boar seminal plasma or hen's egg yolk decrease the in-vitro chemotactic and phagocytotic activities of neutrophils when co-incubated with boar or bull sperm

scientific article published on 26 August 2011

Both fertilization promoting peptide and adenosine stimulate capacitation but inhibit spontaneous acrosome loss in ejaculated boar spermatozoa in vitro

scientific article published on 01 January 2000

Caffeine, dibutyryl cyclic-AMP and heparin affect the chemotactic and phagocytotic activities of neutrophils for boar sperm in vitro

scientific article published on 04 February 2011

Chlortetracycline fluorescence patterns andin vitrofertilisation of frozen-thawed boar spermatozoa incubated under various bicarbonate concentrations ⬇️

scientific article published on May 1, 1997

Development competence and relative transcript abundance of oocytes derived from small and medium follicles of prepubertal gilts.

scientific article published on 27 August 2013

Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity

scientific article published on 01 January 1994

Developmental ability of porcine oocytes matured and fertilized in vitro.

scientific article published in January 1994

Developmental changes in the intracellular Ca2+ release mechanisms in porcine oocytes.

scientific article published in April 1997

Different hormonal requirements of pig oocyte-cumulus complexes during maturation in vitro.

scientific article

Effect of beta-mercaptoethanol during in vitro fertilization procedures on sperm penetration into porcine oocytes and the early development in vitro.

scientific article published in December 2005

Effect of glucose and pyruvate on nuclear and cytoplasmic maturation of porcine oocytes in a chemically defined medium.

scientific article published on 26 July 2008

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes.

scientific article

Effect of the addition of beta-mercaptoethanol to a thawing solution supplemented with caffeine on the function of frozen-thawed boar sperm and on the fertility of sows after artificial insemination

scientific article published on 23 November 2011

Effects of caffeine on sperm characteristics after thawing and inflammatory response in the uterus after artificial insemination with frozen-thawed boar semen.

scientific article published on 30 October 2012

Effects of different serum supplements in maturation medium on meiotic and cytoplasmic maturation of pig oocytes.

scientific article published in April 1993

Effects of electrical stimulation before or after in vitro fertilization on sperm penetration and pronuclear formation of pig oocytes.

scientific article published in November 1993

Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes.

scientific article published in September 1993

Effects of injecting calcium chloride into in vitro-matured porcine oocytes.

scientific article published in February 1996

Exogenous adenosine reduces the mitochondrial membrane potential of murine oocytes during the latter half of in vitro maturation and pronuclear formation following chemical activation.

scientific article published on 6 February 2009

Fertilization and early cleavage in vitro of ageing bovine oocytes after maturation in culture.

scientific article published in March 1992

First cleavage of enucleated rat eggs following transplantation of karyoplast removed from pronuclear eggs stored at 2 to 6 degrees C for various durations

scientific article published on 01 September 1991

Glycosaminoglycans improves early development of zona-free 8-cell rat embryos to blastocysts in a chemically defined medium, but not the pregnancy rate following transfer of the blastocysts.

scientific article published on 2 March 2012

Hydrophobic silicone elastomer chamber for recording trajectories of motile porcine sperms without adsorption

scientific article published on 12 October 2010

Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl2.

scientific article published on 7 September 2009

In vitro development of in vitro-matured porcine oocytes following chemical activation or in vitro fertilization.

scientific article

In vitro development of non-enucleated rat oocytes following microinjection of a cumulus nucleus and chemical activation.

scientific article published in May 2008

In vitro maturation and fertilization of porcine oocytes after a 48 h culture in roscovitine, an inhibitor of p34cdc2/cyclin B kinase.

scientific article published on 28 July 2005

In-vitro culture with a tilting device in chemically defined media during meiotic maturation and early development improves the quality of blastocysts derived from in-vitro matured and fertilized porcine oocytes

scientific article published on 16 June 2010

Induction of capacitation and the acrosome reaction of boar spermatozoa by L-arginine and nitric oxide synthesis associated with the anion transport system.

scientific article published in December 2002

In vitro fertilization in pigs: New molecules and protocols to consider in the forthcoming years.

scientific article published on 23 July 2015

Levels of cyclic-AMP and cyclic-GMP in porcine oocyte-cumulus complexes and cumulus-free oocytes derived from small and middle follicles during the first 24-hour period of in vitro maturation.

scientific article published on 23 February 2017

Methods for Improving In Vitro and In Vivo Boar Sperm Fertility.

scientific article published on July 2015

Microtubule organization in porcine oocytes during fertilization and parthenogenesis.

scientific article published on June 1996

Modulation of the function of boar spermatozoa via adenosine and fertilization promoting peptide receptors reduce the incidence of polyspermic penetration into porcine oocytes.

scientific article published in October 2000

Nuclear transfer of blastomeres expressing EGFP-reporter gene may improve the efficiency of transgenic cattle.

scientific article published in January 2001

Polyspermic penetration in porcine IVM-IVF systems.

scientific article

Preincubation of cumulus-oocyte complexes before exposure to gonadotropins improves the developmental competence of porcine embryos matured and fertilized in vitro.

scientific article published in February 1997

Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles.

scientific article published on 12 December 2016

Production of plasminogen activators (PAs) in bovine cumulus-oocyte complexes during maturation in vitro: effects of epidermal growth factor on production of PAs in oocytes and cumulus cells.

scientific article published in July 1999

Pronuclear visibility, development and transgene expression in IVM/IVF-derived porcine embryos

scientific article published on 01 August 1995

Rapid thawing and stabilizing procedure improve postthaw survival and in vitro penetrability of boar spermatozoa cryopreserved with a glycerol-free trehalose-based extender

scientific article published on 06 June 2015

Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage.

scientific article published in October 1998

Simple vitrification for small numbers of human spermatozoa.

scientific article published on 2 December 2011

Stage-Specific Requirement of Cysteine during in Vitro Maturation of Porcine Oocytes for Glutathione Synthesis Associated with Male Pronuclear Formation1 ⬇️

scientific article published on July 1, 1997

Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia ⬇️

scientific article published on August 15, 2012

Successful piglet production in a chemically defined system for in-vitro production of porcine embryos: dibutyryl cyclic amp and epidermal growth factor-family peptides support in-vitro maturation of oocytes in the absence of gonadotropins.

scientific article published on 14 May 2009

Supplementation with cumulus cell masses improves the in vitro meiotic competence of porcine cumulus-oocytes complexes derived from small follicles.

scientific article published on 30 March 2017

Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization.

scientific article published in July 1997

What is the optimal condition for fertilization of IVM oocytes?

scientific article

Zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy.

scientific article published in November 2000

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