List of works - Hideo Nishitani

A dual-specificity phosphatase Cdc25B is an unstable protein and triggers p34(cdc2)/cyclin B activation in hamster BHK21 cells arrested with hydroxyurea.

scientific article

A hamster temperature-sensitive G1 mutant, tsBN250 has a single point mutation in histidyl-tRNA synthetase that inhibits an accumulation of cyclin D1.

scientific article published on December 1996

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system.

scientific article published on 10 April 2018

CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation

scientific article

Caffeine mimics adenine and 2′‐deoxyadenosine, both of which inhibit the guanine‐nucleotide exchange activity of RCC1 and the kinase activity of ATR ⬇️

scientific article published on May 1, 2003

Cdc18 transcription and proteolysis couple S phase to passage through mitosis ⬇️

scientific article published on October 1, 1998

Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines

scientific article published on August 2004

Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin

scientific article

Cdt1 is differentially targeted for degradation by anticancer chemotherapeutic drugs

scientific article

Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding

scientific article

Cell cycle-dependent subcellular translocation of the human DNA licensing inhibitor geminin.

scientific article

Checkpoint kinase ATR phosphorylates Cdt2, a substrate receptor of CRL4 ubiquitin ligase, and promotes the degradation of Cdt1 following UV irradiation.

scientific article

Chromatin fractionation analysis of licensing factors in mammalian cells.

scientific article published in January 2014

Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm ⬇️

scientific article published on December 1, 1992

Cloning of Xenopus RCC1 cDNA, a homolog of the human RCC1 gene: complementation of tsBN2 mutation and identification of the product.

scientific article

Control of Genome Integrity by RFC Complexes; Conductors of PCNA Loading onto and Unloading from Chromatin during DNA Replication

scientific article published on 26 January 2017

Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration ⬇️

scientific article published on October 1, 1995

Direct binding of Cdt2 to PCNA is important for targeting the CRL4 E3 ligase activity to Cdt1

scientific article published on 31 December 2018

Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma

scientific article published on 29 January 2007

Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction

scientific article published on 27 June 2007

Imaging analysis of cell cycle-dependent degradation of Cdt1 in mammalian cells

scientific article published on 01 January 2014

Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob.

scientific article

Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe

scientific article published on 01 January 2006

Mismatch repair proteins recruited to ultraviolet light-damaged sites lead to degradation of licensing factor Cdt1 in the G1 phase

scientific article

Mitotic UV irradiation induces a DNA replication-licensing defect that potentiates G1 arrest response

scientific article published on 23 March 2015

Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.

scientific article published on February 2003

Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase

scientific article published on 9 February 2018

Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe

scientific article

PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells.

scientific article

Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair.

scientific article

Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.

scholarly article

Positively charged residues located downstream of PIP box, together with TD amino acids within PIP box, are important for CRL4(Cdt2) -mediated proteolysis

scientific article published on 09 December 2010

Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene

scientific article

Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.

scientific article

RCC1, a regulator of mitosis, is essential for DNA replication ⬇️

scientific article published on August 1, 1992

RanBP9 modulates AICD localization and transcriptional activity via direct interaction with Tip60.

scientific article published in January 2014

START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.

scientific article published on 23 January 2009

Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation.

scientific article published on 18 August 2004

Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran

scientific article

Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway

scientific article published on 13 December 2006

The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA.

scientific article

The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase

scientific article (publication date: 30 November 2001)

Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms.

scientific article

Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.

scientific article

Two Ubiquitin-Conjugating Enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, Have Distinct Functions for Ubiquitination of Mitotic Cyclin ⬇️

scientific article published on May 1, 2003

Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation.

scientific article

When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin

scientific article

p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast

scientific article published on 01 November 1995

tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.

scientific article published on April 1992

List of works by Hideo Nishitani

A dual-specificity phosphatase Cdc25B is an unstable protein and triggers p34(cdc2)/cyclin B activation in hamster BHK21 cells arrested with hydroxyurea.

A hamster temperature-sensitive G1 mutant, tsBN250 has a single point mutation in histidyl-tRNA synthetase that inhibits an accumulation of cyclin D1.

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system.

CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation

Caffeine mimics adenine and 2′‐deoxyadenosine, both of which inhibit the guanine‐nucleotide exchange activity of RCC1 and the kinase activity of ATR ⬇️

Cdc18 transcription and proteolysis couple S phase to passage through mitosis ⬇️

Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines

Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin

Cdt1 is differentially targeted for degradation by anticancer chemotherapeutic drugs

Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding

Cell cycle-dependent subcellular translocation of the human DNA licensing inhibitor geminin.

Checkpoint kinase ATR phosphorylates Cdt2, a substrate receptor of CRL4 ubiquitin ligase, and promotes the degradation of Cdt1 following UV irradiation.

Chromatin fractionation analysis of licensing factors in mammalian cells.

Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm ⬇️

Cloning of Xenopus RCC1 cDNA, a homolog of the human RCC1 gene: complementation of tsBN2 mutation and identification of the product.

Control of Genome Integrity by RFC Complexes; Conductors of PCNA Loading onto and Unloading from Chromatin during DNA Replication

Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration ⬇️

Direct binding of Cdt2 to PCNA is important for targeting the CRL4 E3 ligase activity to Cdt1

Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma

Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction

Imaging analysis of cell cycle-dependent degradation of Cdt1 in mammalian cells

Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob.

Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe

Mismatch repair proteins recruited to ultraviolet light-damaged sites lead to degradation of licensing factor Cdt1 in the G1 phase

Mitotic UV irradiation induces a DNA replication-licensing defect that potentiates G1 arrest response

Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.

Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase

Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe

PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells.

Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair.

Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.

Positively charged residues located downstream of PIP box, together with TD amino acids within PIP box, are important for CRL4(Cdt2) -mediated proteolysis

Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene

Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.

RCC1, a regulator of mitosis, is essential for DNA replication ⬇️

RanBP9 modulates AICD localization and transcriptional activity via direct interaction with Tip60.

START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.

Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation.

Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran

Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway

The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into duplex DNA.

The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase

Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms.

Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.

Two Ubiquitin-Conjugating Enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, Have Distinct Functions for Ubiquitination of Mitotic Cyclin ⬇️

Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation.

When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin

p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast

tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.