List of works - Miguel de Vega

A conserved insertion in protein-primed DNA polymerases is involved in primer terminus stabilisation.

scientific article

An array of basic residues is essential for the nucleolytic activity of the PHP domain of bacterial/archaeal PolX DNA polymerases

scientific article published on 09 July 2019

An aspartic acid residue in TPR-1, a specific region of protein-priming DNA polymerases, is required for the functional interaction with primer terminal protein

scientific article published on 01 December 2000

An invariant lysine residue is involved in catalysis at the 3′-5′ exonuclease active site of eukaryotic-type DNA polymerases ⬇️

scientific article published on July 4, 1997

Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining

scientific article published in June 2019

DNA polymerase from temperate phage Bam35 is endowed with processive polymerization and abasic sites translesion synthesis capacity.

scientific article published on 22 June 2015

DNA stabilization at the Bacillus subtilis PolX core--a binding model to coordinate polymerase, AP-endonuclease and 3'-5' exonuclease activities ⬇️

scientific article published on July 25, 2012

DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication

scientific article

Dual role of φ29 DNA polymerase Lys529 in stabilisation of the DNA priming-terminus and the terminal protein-priming residue at the polymerisation site

scientific article

Efficient processing of abasic sites by bacterial nonhomologous end-joining Ku proteins

scientific article published on 29 October 2014

Identification of a conserved 5'-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair.

scientific article published on 29 January 2016

Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs ⬇️

scientific article published on September 7, 2010

Intrinsic apurinic/apyrimidinic (AP) endonuclease activity enables Bacillus subtilis DNA polymerase X to recognize, incise, and further repair abasic sites ⬇️

scientific article published on October 25, 2010

Involvement of residues of the ϕ29 terminal protein intermediate and priming domains in the formation of a stable and functional heterodimer with the replicative DNA polymerase ⬇️

scientific article published on December 30, 2011

Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding.

scientific article

Mutational analysis of ø29 DNA polymerase residues acting as ssDNA ligands for 3′-5′ exonucleolysis ⬇️

scientific article published on June 19, 1998

New insights into the coordination between the polymerization and 3'-5' exonuclease activities in ϕ29 DNA polymerase

scientific article published in Scientific Reports

Noncatalytic aspartate at the exonuclease domain of proofreading DNA polymerases regulates both degradative and synthetic activities

article published in the Proceedings of the National Academy of Sciences of the United States of America

Phaeocystis globosa Virus DNA Polymerase X: a "Swiss Army knife", Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair.

scientific article

Phage phi 29 DNA polymerase residues involved in the proper stabilisation of the primer-terminus at the 3'-5' exonuclease active site

scientific article published on 01 November 2000

Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase.

scientific article

Structural Determinants Responsible for the Preferential Insertion of Ribonucleotides by Bacterial NHEJ PolDom

scientific article published on 30 January 2020

Terminal protein-primed DNA amplification

scientific article

Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage Phi29.

scientific article published on 7 November 2011

The Loop of the TPR1 Subdomain of Phi29 DNA Polymerase Plays a Pivotal Role in Primer-Terminus Stabilization at the Polymerization Active Site

scientific article published on 24 October 2019

The anti/syn conformation of 8-oxo-7,8-dihydro-2'-deoxyguanosine is modulated by Bacillus subtilis PolX active site residues His255 and Asn263. Efficient processing of damaged 3'-ends.

scientific article published on 16 February 2017

The essential role of the 3' terminal template base in the first steps of protein-primed DNA replication

scientific article

The minimal Bacillus subtilis nonhomologous end joining repair machinery

scientific article

phi 29 DNA polymerase residue Leu384, highly conserved in motif B of eukaryotic type DNA replicases, is involved in nucleotide insertion fidelity.

scientific article

List of works by Miguel de Vega

A conserved insertion in protein-primed DNA polymerases is involved in primer terminus stabilisation.

An array of basic residues is essential for the nucleolytic activity of the PHP domain of bacterial/archaeal PolX DNA polymerases

An aspartic acid residue in TPR-1, a specific region of protein-priming DNA polymerases, is required for the functional interaction with primer terminal protein

An invariant lysine residue is involved in catalysis at the 3′-5′ exonuclease active site of eukaryotic-type DNA polymerases ⬇️

Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining

DNA polymerase from temperate phage Bam35 is endowed with processive polymerization and abasic sites translesion synthesis capacity.

DNA stabilization at the Bacillus subtilis PolX core--a binding model to coordinate polymerase, AP-endonuclease and 3'-5' exonuclease activities ⬇️

DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication

Dual role of φ29 DNA polymerase Lys529 in stabilisation of the DNA priming-terminus and the terminal protein-priming residue at the polymerisation site

Efficient processing of abasic sites by bacterial nonhomologous end-joining Ku proteins

Identification of a conserved 5'-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair.

Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs ⬇️

Intrinsic apurinic/apyrimidinic (AP) endonuclease activity enables Bacillus subtilis DNA polymerase X to recognize, incise, and further repair abasic sites ⬇️

Involvement of residues of the ϕ29 terminal protein intermediate and priming domains in the formation of a stable and functional heterodimer with the replicative DNA polymerase ⬇️

Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding.

Mutational analysis of ø29 DNA polymerase residues acting as ssDNA ligands for 3′-5′ exonucleolysis ⬇️

New insights into the coordination between the polymerization and 3'-5' exonuclease activities in ϕ29 DNA polymerase

Noncatalytic aspartate at the exonuclease domain of proofreading DNA polymerases regulates both degradative and synthetic activities

Phaeocystis globosa Virus DNA Polymerase X: a "Swiss Army knife", Multifunctional DNA polymerase-lyase-ligase for Base Excision Repair.

Phage phi 29 DNA polymerase residues involved in the proper stabilisation of the primer-terminus at the 3'-5' exonuclease active site

Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase.

Structural Determinants Responsible for the Preferential Insertion of Ribonucleotides by Bacterial NHEJ PolDom

Terminal protein-primed DNA amplification

Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage Phi29.

The Loop of the TPR1 Subdomain of Phi29 DNA Polymerase Plays a Pivotal Role in Primer-Terminus Stabilization at the Polymerization Active Site

The anti/syn conformation of 8-oxo-7,8-dihydro-2'-deoxyguanosine is modulated by Bacillus subtilis PolX active site residues His255 and Asn263. Efficient processing of damaged 3'-ends.

The essential role of the 3' terminal template base in the first steps of protein-primed DNA replication

The minimal Bacillus subtilis nonhomologous end joining repair machinery

phi 29 DNA polymerase residue Leu384, highly conserved in motif B of eukaryotic type DNA replicases, is involved in nucleotide insertion fidelity.