List of works - Amir Aharoni

A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in <i>Saccharomyces cerevisiae</i>

scientific article published on 16 September 2021

A Live-Cell Imaging Approach for Measuring DNA Replication Rates

scientific article published on 01 July 2018

A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells.

scientific article published on 19 June 2018

A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities.

scientific article

Application of directed evolution and back-to-consensus algorithms to human alpha1-antitrypsin leads to diminished anti-protease activity and augmented anti-inflammatory activities

scientific article published on 21 May 2020

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

scientific article published on 14 July 2016

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

scientific article

Bacteriorhodpsin experiences light-induced conformational alterations in nonisomerizable C(13)=C(14) pigments. A study with EPR

scientific article published on 01 July 2000

Bioengineering: Evolved to overcome Bt-toxin resistance

scientific article published on 27 April 2016

Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability

scientific article published on 06 October 2020

Coevolution predicts direct interactions between mtDNA-encoded and nDNA-encoded subunits of oxidative phosphorylation complex i

scientific article published on 22 September 2010

Computational protein design suggests that human PCNA-partner interactions are not optimized for affinity.

scientific article

Condition-specific genetic interaction maps reveal crosstalk between the cAMP/PKA and the HOG MAPK pathways in the activation of the general stress response

scientific article

Conserved motifs in the Msn2-activating domain are important for Msn2-mediated yeast stress response

article

Covalent inhibition of bacterial quorum sensing.

scientific article published in August 2009

Directed evolution of SIRT6 for improved deacylation and glucose homeostasis maintenance

scientific article published on 23 February 2018

Directed evolution of a soluble human DR3 receptor for the inhibition of TL1A induced cytokine secretion

scientific article

Directed evolution of a soluble human IL-17A receptor for the inhibition of psoriasis plaque formation in a mouse model.

scientific article published in February 2013

Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization

scientific article

Directed evolution of proteins for heterologous expression and stability.

scientific article

Directed evolution of recombinant serum paraoxonase (PON) variants

scientific article published on April 2004

Dissecting the roles of the N- and C-flanking residues of acetyllysine substrates for SIRT1 activity

scientific article published on 20 February 2013

Employing directed evolution for the functional analysis of multi-specific proteins

scientific article published on 27 April 2013

Engineering of Methylation State Specific 3xMBT Domain Using ELISA Screening

scientific article

Enhanced PKMT-substrate recognition through non active-site interactions

scientific article published on 24 May 2018

Evolution in microfluidic droplet

scientific article published on 01 August 2012

Experimental analysis of co-evolution within protein complexes: the yeast exosome as a model

scientific article

Fine-tuning of the Msn2/4-mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners

scientific article

Fusion peptides derived from the HIV type 1 glycoprotein 41 associate within phospholipid membranes and inhibit cell-cell Fusion. Structure-function study.

scientific article published in May 1997

Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness

scientific article

Harnessing the oxidation susceptibility of deubiquitinases for inhibition with small molecules

scientific article published on 19 October 2014

High-throughput Screens and Selections of Enzyme-encoding Genes

scientific article published on 5 May 2006

High-throughput screening methodology for the directed evolution of glycosyltransferases

scientific article

High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.

scientific article

High-throughput screens and selections of enzyme-encoding genes.

scientific article

Human SIRT1 multi-specificity is modulated by active-site vicinity substitutions during natural evolution

scientific article published on 21 September 2020

Increased Potency of a Bi-specific TL1A-ADAM17 (TACE) Inhibitor by Cell Surface Targeting.

scientific article published on 22 August 2017

Inter-ring communication is disrupted in the GroEL mutant Arg13 --> Gly; Ala126 --> Val with known crystal structure.

scientific article published in May 1996

Interplay of metagenomics and in vitro compartmentalization.

scientific article

Light-induced hydrolysis and rebinding of nonisomerizable bacteriorhodopsin pigment

scientific article

Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.

scientific article published on 6 April 2018

Mining for new enzymes

scientific article published on March 2009

Novel hybrid esterase-haloacid dehalogenase enzyme

scientific article published on 01 September 2010

Photoreduction of bacteriorhodopsin Schiff base at low humidity. A study with C13=C14 nonisomerizable artificial pigments

scientific article

Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures

scientific article published on 01 December 2018

Point Mutation of a Non-Elastase-Binding Site in Human α1-Antitrypsin Alters Its Anti-Inflammatory Properties.

scientific article

Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44.

scientific article

Proteasome accessory factor A (PafA) transferase activity makes sense in the light of its homology with glutamine synthetase.

scientific article published in February 2018

Protein co-evolution: how do we combine bioinformatics and experimental approaches?

scientific article

Sequential switching of binding partners on PCNA during in vitro Okazaki fragment maturation

scientific article

Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes

scientific article

Subtle alterations in PCNA-partner interactions severely impair DNA replication and repair

scientific article

Surface display of redox enzymes in microbial fuel cells.

scientific article published in September 2009

Targeting deubiquitinases enabled by chemical synthesis of proteins.

scientific article

The 'evolvability' of promiscuous protein functions

scientific article

The Molecular Basis for the Broad Substrate Specificity of Human Sulfotransferase 1A1

scientific article

The evolution of a G1/S transcriptional network in yeasts

scientific article published on 25 July 2017

The functional importance of co-evolving residues in proteins.

scientific article published on September 2013

The transition of human estrogen sulfotransferase from generalist to specialist using directed enzyme evolution

scientific article

Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility

scientific article published on 17 January 2012

Transcription-replication coordination revealed in single live cells

scientific article published on 01 February 2022

List of works by Amir Aharoni

A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in <i>Saccharomyces cerevisiae</i>

A Live-Cell Imaging Approach for Measuring DNA Replication Rates

A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells.

A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities.

Application of directed evolution and back-to-consensus algorithms to human alpha1-antitrypsin leads to diminished anti-protease activity and augmented anti-inflammatory activities

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.

Bacteriorhodpsin experiences light-induced conformational alterations in nonisomerizable C(13)=C(14) pigments. A study with EPR

Bioengineering: Evolved to overcome Bt-toxin resistance

Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability

Coevolution predicts direct interactions between mtDNA-encoded and nDNA-encoded subunits of oxidative phosphorylation complex i

Computational protein design suggests that human PCNA-partner interactions are not optimized for affinity.

Condition-specific genetic interaction maps reveal crosstalk between the cAMP/PKA and the HOG MAPK pathways in the activation of the general stress response

Conserved motifs in the Msn2-activating domain are important for Msn2-mediated yeast stress response

Covalent inhibition of bacterial quorum sensing.

Directed evolution of SIRT6 for improved deacylation and glucose homeostasis maintenance

Directed evolution of a soluble human DR3 receptor for the inhibition of TL1A induced cytokine secretion

Directed evolution of a soluble human IL-17A receptor for the inhibition of psoriasis plaque formation in a mouse model.

Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization

Directed evolution of proteins for heterologous expression and stability.

Directed evolution of recombinant serum paraoxonase (PON) variants

Dissecting the roles of the N- and C-flanking residues of acetyllysine substrates for SIRT1 activity

Employing directed evolution for the functional analysis of multi-specific proteins

Engineering of Methylation State Specific 3xMBT Domain Using ELISA Screening

Enhanced PKMT-substrate recognition through non active-site interactions

Evolution in microfluidic droplet

Experimental analysis of co-evolution within protein complexes: the yeast exosome as a model

Fine-tuning of the Msn2/4-mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners

Fusion peptides derived from the HIV type 1 glycoprotein 41 associate within phospholipid membranes and inhibit cell-cell Fusion. Structure-function study.

Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness

Harnessing the oxidation susceptibility of deubiquitinases for inhibition with small molecules

High-throughput Screens and Selections of Enzyme-encoding Genes

High-throughput screening methodology for the directed evolution of glycosyltransferases

High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.

High-throughput screens and selections of enzyme-encoding genes.

Human SIRT1 multi-specificity is modulated by active-site vicinity substitutions during natural evolution

Increased Potency of a Bi-specific TL1A-ADAM17 (TACE) Inhibitor by Cell Surface Targeting.

Inter-ring communication is disrupted in the GroEL mutant Arg13 --> Gly; Ala126 --> Val with known crystal structure.

Interplay of metagenomics and in vitro compartmentalization.

Light-induced hydrolysis and rebinding of nonisomerizable bacteriorhodopsin pigment

Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.

Mining for new enzymes

Novel hybrid esterase-haloacid dehalogenase enzyme

Photoreduction of bacteriorhodopsin Schiff base at low humidity. A study with C13=C14 nonisomerizable artificial pigments

Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures

Point Mutation of a Non-Elastase-Binding Site in Human α1-Antitrypsin Alters Its Anti-Inflammatory Properties.

Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44.

Proteasome accessory factor A (PafA) transferase activity makes sense in the light of its homology with glutamine synthetase.

Protein co-evolution: how do we combine bioinformatics and experimental approaches?

Sequential switching of binding partners on PCNA during in vitro Okazaki fragment maturation

Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes

Subtle alterations in PCNA-partner interactions severely impair DNA replication and repair

Surface display of redox enzymes in microbial fuel cells.

Targeting deubiquitinases enabled by chemical synthesis of proteins.

The 'evolvability' of promiscuous protein functions

The Molecular Basis for the Broad Substrate Specificity of Human Sulfotransferase 1A1

The evolution of a G1/S transcriptional network in yeasts

The functional importance of co-evolving residues in proteins.

The transition of human estrogen sulfotransferase from generalist to specialist using directed enzyme evolution

Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility

Transcription-replication coordination revealed in single live cells