List of works - M.R. Aires-Barros

A continuous membrane bioreactor for ester synthesis in organic media: I. Operational characterization and stability.

scientific article published in January 2001

A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems

scientific article

A new look at xylanases: an overview of purification strategies

scientific article (publication date: July 2003)

A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning

scientific article published on 21 August 2011

A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples.

scientific article published on 12 September 2016

A simple method for point-of-need extraction, concentration and rapid multi-mycotoxin immunodetection in feeds using aqueous two-phase systems.

scientific article published on 5 July 2017

Affinity partitioning of human antibodies in aqueous two-phase systems.

scientific article published on 21 March 2007

Affinity-enhanced purification of human antibodies by aqueous two-phase extraction

article published in 2009

Application of aqueous two-phase systems to antibody purification: a multi-stage approach.

scientific article published in February 2009

Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies.

scientific article published on 28 December 2006

Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6

Aqueous two-phase extraction as a platform in the biomanufacturing industry: economical and environmental sustainability.

scientific article published on 08 April 2011

Aqueous two-phase systems for enhancing immunoassay sensitivity: simultaneous concentration of mycotoxins and neutralization of matrix interference.

scientific article published on 12 August 2014

Biotransformation in organic media by enzymes and whole cells

article

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.

scientific article published on November 2010

Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions.

scientific article published on 18 November 2012

Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing.

scientific article published on 27 February 2009

Conformational states of HRPA1 induced by thermal unfolding: effect of low molecular weight solutes.

scientific article published in October 2004

Continuous aqueous two-phase extraction of human antibodies using a packed column.

scientific article published on 30 November 2011

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: from concept to process.

scientific article published on 17 January 2013

Cutinase purification on poly(ethylene glycol)–hydroxypropyl starch aqueous two-phase systems

scientific article published on 01 June 1998

Deactivation and conformational changes of cutinase in reverse micelles

Design of a microfluidic platform for monoclonal antibody extraction using an aqueous two-phase system

scientific article published on 12 June 2012

Determination of aqueous two phase system binodal curves using a microfluidic device

scientific article published on 27 October 2014

Determination of partition coefficients of biomolecules in a microfluidic aqueous two phase system platform using fluorescence microscopy

scientific article published on 15 December 2016

Downstream processing of antibodies: single-stage versus multi-stage aqueous two-phase extraction.

scientific article published on 13 February 2009

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

scientific article published on 14 May 2009

Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution

scientific article published on 01 June 1998

Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET

scientific article

Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch

scientific article published on 01 January 2000

Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi

Effects of ionic surfactants used in reversed micelles on cutinase activity and stability.

scientific article

Enzymatic esterification of ethanol by an immobilised Rhizomucor miehei lipase in a perforated rotating disc bioreactor.

scientific article published in March 2000

Enzymatic properties of a neutral endo-1,3(4)-beta-xylanase Xyl II from Bacillus subtilis

scientific article published on 01 April 2002

Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles.

scientific article published on 20 July 2012

Fluorescence methods for lipoplex characterization.

scientific article published on 29 July 2011

Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium.

scientific article

High-Throughput Nanoliter-Scale Analysis and Optimization of Multimodal Chromatography for the Capture of Monoclonal Antibodies.

scientific article

Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography.

scientific article

Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins.

scientific article published in January 2003

Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite

Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: modeling of a batch stirred tank reactor.

scientific article published in July 2000

Miniaturization of aqueous two-phase extraction for biological applications: From micro-tubes to microchannels

scientific article published on 14 September 2016

Modeling the partitioning of amino acids in aqueous two phase systems

scientific article published on 16 December 2013

Molecular construction of bionanoparticles: chimaeric SIV p17-HIV I p6 nanoparticles with minimal viral protein content.

scientific article published on September 2007

On-chip sample preparation and analyte quantification using a microfluidic aqueous two-phase extraction coupled with an immunoassay

scientific article published in November 2014

Optimisation of aqueous two-phase extraction of human antibodies.

scientific article published on 24 April 2007

Optimizing the Performance of Chromatographic Separations Using Microfluidics: Multiplexed and Quantitative Screening of Ligands and Target Molecules

scientific article published on 23 July 2019

Partitioning in aqueous two-phase systems: Analysis of strengths, weaknesses, opportunities and threats.

scientific article published on 24 July 2015

Phenylboronate chromatography selectively separates glycoproteins through the manipulation of electrostatic, charge transfer, and cis-diol interactions

scientific article

Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: development and optimization of a washing step.

scientific article published on 6 June 2014

Photophysical Behavior of a Dimeric Cyanine Dye (BOBO-1) Within Cationic Liposomes

scientific article published on 01 November 2005

Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions

article

Predicting protein partition coefficients in aqueous two phase system

scientific article published in October 2016

Predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems

scientific article published on 01 November 1997

Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems.

scientific article published on 8 May 2008

Purification of lipases

scientific article published on November 1, 1992

Salt effects on solvent features of coexisting phases in aqueous polymer/polymer two-phase systems

scientific article published on 18 January 2012

Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase

scientific article published on 01 January 2000

Solvatochromic relationship: prediction of distribution of ionic solutes in aqueous two-phase systems.

scientific article published on 19 November 2012

Stability and proteolytic activity of papain in reverse micellar and aqueous media: a kinetic and spectroscopic study

scientific article published on 01 July 1994

Stability of a recombinant cutinase immobilized on zeolites

Stimuli-responsive magnetic nanoparticles for monoclonal antibody purification.

scientific article

Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads.

scientific article

Study of organic compounds-water interactions by partition in aqueous two-phase systems.

scientific article

The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules.

scientific article

Thermal unfolding of proteins at high pH range studied by UV absorbance

scientific article published on 01 February 1997

Triglyceride hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles

scientific article published on 01 January 1995

Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography.

scientific article published in November 2012

List of works by M.R. Aires-Barros

A continuous membrane bioreactor for ester synthesis in organic media: I. Operational characterization and stability.

A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems

A new look at xylanases: an overview of purification strategies

A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning

A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples.

A simple method for point-of-need extraction, concentration and rapid multi-mycotoxin immunodetection in feeds using aqueous two-phase systems.

Affinity partitioning of human antibodies in aqueous two-phase systems.

Affinity-enhanced purification of human antibodies by aqueous two-phase extraction

Application of aqueous two-phase systems to antibody purification: a multi-stage approach.

Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies.

Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6

Aqueous two-phase extraction as a platform in the biomanufacturing industry: economical and environmental sustainability.

Aqueous two-phase systems for enhancing immunoassay sensitivity: simultaneous concentration of mycotoxins and neutralization of matrix interference.

Biotransformation in organic media by enzymes and whole cells

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.

Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions.

Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing.

Conformational states of HRPA1 induced by thermal unfolding: effect of low molecular weight solutes.

Continuous aqueous two-phase extraction of human antibodies using a packed column.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: from concept to process.

Cutinase purification on poly(ethylene glycol)–hydroxypropyl starch aqueous two-phase systems

Deactivation and conformational changes of cutinase in reverse micelles

Design of a microfluidic platform for monoclonal antibody extraction using an aqueous two-phase system

Determination of aqueous two phase system binodal curves using a microfluidic device

Determination of partition coefficients of biomolecules in a microfluidic aqueous two phase system platform using fluorescence microscopy

Downstream processing of antibodies: single-stage versus multi-stage aqueous two-phase extraction.

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution

Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET

Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch

Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi

Effects of ionic surfactants used in reversed micelles on cutinase activity and stability.

Enzymatic esterification of ethanol by an immobilised Rhizomucor miehei lipase in a perforated rotating disc bioreactor.

Enzymatic properties of a neutral endo-1,3(4)-beta-xylanase Xyl II from Bacillus subtilis

Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles.

Fluorescence methods for lipoplex characterization.

Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium.

High-Throughput Nanoliter-Scale Analysis and Optimization of Multimodal Chromatography for the Capture of Monoclonal Antibodies.

Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography.

Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins.

Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite

Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: modeling of a batch stirred tank reactor.

Miniaturization of aqueous two-phase extraction for biological applications: From micro-tubes to microchannels

Modeling the partitioning of amino acids in aqueous two phase systems

Molecular construction of bionanoparticles: chimaeric SIV p17-HIV I p6 nanoparticles with minimal viral protein content.

On-chip sample preparation and analyte quantification using a microfluidic aqueous two-phase extraction coupled with an immunoassay

Optimisation of aqueous two-phase extraction of human antibodies.

Optimizing the Performance of Chromatographic Separations Using Microfluidics: Multiplexed and Quantitative Screening of Ligands and Target Molecules

Partitioning in aqueous two-phase systems: Analysis of strengths, weaknesses, opportunities and threats.

Phenylboronate chromatography selectively separates glycoproteins through the manipulation of electrostatic, charge transfer, and cis-diol interactions

Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: development and optimization of a washing step.

Photophysical Behavior of a Dimeric Cyanine Dye (BOBO-1) Within Cationic Liposomes

Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions

Predicting protein partition coefficients in aqueous two phase system

Predicting the partition coefficients of a recombinant cutinase in polyethylene glycol/phosphate aqueous two-phase systems

Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems.

Purification of lipases

Salt effects on solvent features of coexisting phases in aqueous polymer/polymer two-phase systems

Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase

Solvatochromic relationship: prediction of distribution of ionic solutes in aqueous two-phase systems.

Stability and proteolytic activity of papain in reverse micellar and aqueous media: a kinetic and spectroscopic study

Stability of a recombinant cutinase immobilized on zeolites

Stimuli-responsive magnetic nanoparticles for monoclonal antibody purification.

Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads.

Study of organic compounds-water interactions by partition in aqueous two-phase systems.

The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules.

Thermal unfolding of proteins at high pH range studied by UV absorbance

Triglyceride hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles

Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography.

Zeolites as supports for an enzymatic alcoholysis reaction

Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites