List of works - Daan van Aalten

A mechanism-inspired UDP-<i>N</i>-acetylglucosamine pyrophosphorylase inhibitor

scientific article published on 24 March 2020

A missense mutation in a patient with developmental delay affects the activity and structure of the hexosamine biosynthetic pathway enzyme AGX1

scientific article published on 24 October 2020

A missense mutation in the catalytic domain of O-GlcNAc transferase links perturbations in protein O-GlcNAcylation to X-linked intellectual disability

scientific article published on 07 November 2019

A mouse model for functional dissection of TAB1 O-GlcNAcylation

scientific article published on 27 August 2019

A mutant O-GlcNAcase enriches Drosophila developmental regulators.

scientific article published on 12 June 2017

A structural and biochemical model of processive chitin synthesis

scientific article

A structural snapshot of base-pair opening in DNA

scientific article

An efficient synthesis of argifin: a natural product chitinase inhibitor with chemotherapeutic potential.

scientific article

An intellectual disability syndrome with single-nucleotide variants in O-GlcNAc transferase

scientific article published on 20 February 2020

Analysis of the LKB1-STRAD-MO25 complex

scientific article

Analyzing Airway Inflammation with Chemical Biology: Dissection of Acidic Mammalian Chitinase Function with a Selective Drug-like Inhibitor

scientific article

Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein

scientific article

Bisdionin C-a rationally designed, submicromolar inhibitor of family 18 chitinases

scientific article

Bisubstrate UDP–peptide conjugates as human O-GlcNAc transferase inhibitors

scientific article

BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm.

scientific article

Catalytic deficiency of O-GlcNAc transferase leads to X-linked intellectual disability

scientific article published on 11 July 2019

Charge-Surrounded Pockets and Electrostatic Interactions with Small Ions Modulate the Activity of Retroviral Fusion Proteins ⬇️

scientific article published on February 3, 2011

Chemical Dissection of the Link between Streptozotocin, O-GlcNAc, and Pancreatic Cell Death

scientific article

Chemical tools to probe cellular O-GlcNAc signalling.

scientific article

Comparative structural analysis of retroviral fusion proteins identifies regions that modulate membrane fusion: a potential retroviral achilles heal?

Conformational substates in different crystal forms of the photoactive yellow protein-Correlation with theoretical and experimental flexibility

scientific article

Crystal structure and binding properties of the Serratia marcescens chitin-binding protein CBP21.

scientific article

Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 Å resolution 1 1Edited by R. Huber ⬇️

scientific article published on November 9, 2001

Crystal structures of allosamidin derivatives in complex with human macrophage chitinase

scientific article (publication date: 30 May 2003)

Crystallization and X-ray diffraction analysis of peroxisomal Δ3-Δ2-enoyl-CoA isomerase fromSaccharomyces cerevisiae

scientific article published on 01 August 2000

Crystallization and X-ray diffraction studies of the fatty-acid responsive transcription factor FadR from Escherichia coli

scientific article published on 01 April 2000

Discovery of catalytically active orthologues of the Parkinson's disease kinase PINK1: analysis of substrate specificity and impact of mutations.

scientific article published on November 2011

Domain motions in bacteriophage T4 lysozyme: a comparison between molecular dynamics and crystallographic data.

scientific article

Dual functionality of O-GlcNAc transferase is required for Drosophila development.

scientific article

Dynamic properties of the guanine nucleotide binding protein alpha subunit and comparison of its guanosine triphosphate hydrolase domain with that of ras p21.

scientific article published in March 1998

Efficient synthesis of 1,3,7-substituted xanthines by a safety-catch protection strategy

Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ESC differentiation without affecting naïve to primed cell transition.

scientific article published on October 2014

Essential Dynamics from NMR Clusters: Dynamic Properties of the Myb DNA-Binding Domain and a Hinge-Bending Enhancing Variant ⬇️

scientific article published on March 1, 1998

Essential dynamics of DNA containing a cis.syn cyclobutane thymine dimer lesion.

scientific article

Evidence for a Functional O-Linked N-Acetylglucosamine (O-GlcNAc) System in the Thermophilic Bacterium Thermobaculum terrenum

scientific article

Functional concerted motions in the bovine serum retinol-binding protein

scientific article published on 01 January 1999

Genetic recoding to dissect the roles of site-specific protein O-GlcNAcylation

scientific article published on 06 November 2019

Genetic validation of Aspergillus fumigatus phosphoglucomutase as a viable therapeutic target in invasive aspergillosis

scientific article published on 30 April 2022

GlcNAcstatin: a picomolar, selective O-GlcNAcase inhibitor that modulates intracellular O-glcNAcylation levels

scientific article

GlcNAcstatins are nanomolar inhibitors of human O -GlcNAcase inducing cellular hyper- O -GlcNAcylation

scientific article

Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy

scientific article published on 25 April 2016

Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex

scientific article

High resolution crystal structures of Siglec-7. Insights into ligand specificity in the Siglec family

scientific article

High resolution crystal structures of unliganded and liganded human liver ACBP reveal a new mode of binding for the acyl-CoA ligand

scientific article published on 01 January 2007

Human OGA binds substrates in a conserved peptide recognition groove ⬇️

scientific article published on November 15, 2010

Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties

scientific article

Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone

scientific article

Lead optimization of a pyrazole sulfonamide series of Trypanosoma brucei N-myristoyltransferase inhibitors: identification and evaluation of CNS penetrant compounds as potential treatments for stage 2 human African trypanosomiasis

scientific article

Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice

scientific article published on 27 November 2019

Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases

scientific article published on 10 April 2019

Methylxanthine drugs are chitinase inhibitors: investigation of inhibition and binding modes.

scientific article published in September 2005

Molecular Mechanisms of Yeast Cell Wall Glucan Remodeling

scientific article

Molecular mechanisms of O-GlcNAcylation.

scientific article published on 06 October 2008

Molecular recognition: O-GlcNAc transfer: size matters.

scientific article published on March 2011

Mutation of the PDK1 PH Domain Inhibits Protein Kinase B/Akt, Leading to Small Size and Insulin Resistance

scientific article

Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase.

scientific article

Mutations in N-acetylglucosamine (O-GlcNAc) transferase in patients with X-linked intellectual disability

scientific article published on 05 June 2017

N-myristoyltransferase inhibitors as new leads to treat sleeping sickness

scientific article

N-myristoyltransferase is a cell wall target in Aspergillus fumigatus

scientific article published on 27 February 2015

Natural Product–Guided Discovery of a Fungal Chitinase Inhibitor

scientific article

O-GlcNAcase contributes to cognitive function in Drosophila

scientific article published on 24 February 2020

O-GlcNAcase fragment discovery with fluorescence polarimetry.

scientific article published on 11 April 2018

O-GlcNAcase: promiscuous hexosaminidase or key regulator of O-GlcNAc signaling?

scientific article

O-GlcNAcylation of TAB1 modulates TAK1-mediated cytokine release.

scientific article

PRODRG: a tool for high-throughput crystallography of protein-ligand complexes

scientific article (publication date: August 2004)

Phosphorylation of synaptic vesicle protein 2A at Thr84 by casein kinase 1 family kinases controls the specific retrieval of synaptotagmin-1.

scientific article

Prediction of protein conformational freedom from distance constraints

scientific article published on 01 October 1997

Protein O-GlcNAcylation is required for fibroblast growth factor signaling in Drosophila.

scientific article published on 20 December 2011

Proteome wide purification and identification of O-GlcNAc-modified proteins using click chemistry and mass spectrometry

scientific article

Pseudokinases-remnants of evolution or key allosteric regulators?

scientific article

Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus

scientific article

Purification, crystallization and preliminary X-ray diffraction of a proteolytic fragment of PDK1 containing the pleckstrin homology domain.

scientific article published on 23 January 2004

Putting glycobiology on a structural footing.

scientific article published in October 2008

Recognition of a glycosylation substrate by the O-GlcNAc transferase TPR repeats

scientific article published on June 2017

Screening-based discovery of drug-likeO-GlcNAcase inhibitor scaffolds ⬇️

scientific article

Screening-based discovery ofAspergillus fumigatusplant-type chitinase inhibitors

scientific article

Sequence, chromophore extraction and 3-D model of the photoactive yellow protein from Rhodobacter sphaeroides.

scientific article published on June 1998

Solid-phase synthesis of cyclic peptide chitinase inhibitors: SAR of the argifin scaffold.

scientific article published on 13 November 2008

Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases.

scientific article published in January 2005

Structural and biochemical characterization of a trapped coenzyme A adduct ofCaenorhabditis elegansglucosamine-6-phosphateN-acetyltransferase 1

scientific article

Structural and kinetic differences between human and Aspergillus fumigatus D-glucosamine-6-phosphate N-acetyltransferase

scientific article

Structural basis of reduction-dependent activation of human cystatin F.

scientific article

Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis

scientific article

Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates.

scientific article published on 30 September 2004

Structure and Mechanism of Chitin Deacetylase from the Fungal PathogenColletotrichum lindemuthianum†,‡

scientific article published on 01 August 2006

Structure of PINK1 and mechanisms of Parkinson's disease-associated mutations.

scientific article published on 5 October 2017

Structure of Saccharomyces cerevisiae chitinase 1 and screening-based discovery of potent inhibitors

scientific article

Structure of a bacterial putative acetyltransferase defines the fold of the human O-GlcNAcase C-terminal domain

scientific article

Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin

scientific article

Substrate and product analogues as human O-GlcNAc transferase inhibitors

scientific article

Synergy of Peptide and Sugar in O-GlcNAcase Substrate Recognition

scientific article

TAK1-binding protein 1 is a pseudophosphatase.

scientific article published on November 2006

Targeting a critical step in fungal hexosamine biosynthesis

scientific article published on 27 April 2020

The Early Metazoan Trichoplax adhaerens Possesses a Functional O-GlcNAc System

scientific article

The N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme.

scientific article published on 6 April 2005

The O-GlcNAc Transferase Intellectual Disability Mutation L254F Distorts the TPR Helix.

scientific article published on 23 March 2018

The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces

scientific article

The active site of O-GlcNAc transferase imposes constraints on substrate sequence

scientific article published on 3 August 2015

The conserved threonine-rich region of the HCF-1PRO repeat activates promiscuous OGT:UDP-GlcNAc glycosylation and proteolysis activities

scientific article published in Journal of Biological Chemistry

The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase

scientific article published in June 2001

The essential dynamics of thermolysin: Confirmation of the hinge-bending motion and comparison of simulations in vacuum and water

article

The non-catalytic chitin-binding protein CBP21 from Serratia marcescens is essential for chitin degradation.

scientific article published in June 2005

The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR

scientific article

The structure of siglec-7 in complex with sialosides: leads for rational structure-based inhibitor design

scientific article

UDP-GlcNAc Analogues as Inhibitors of O -GlcNAc Transferase (OGT): Spectroscopic, Computational, and Biological Studies

scientific article published on 30 April 2018

Yeast Mnn9 is both a priming glycosyltransferase and an allosteric activator of mannan biosynthesis

scientific article

List of works by Daan van Aalten

A mechanism-inspired UDP-<i>N</i>-acetylglucosamine pyrophosphorylase inhibitor

A missense mutation in a patient with developmental delay affects the activity and structure of the hexosamine biosynthetic pathway enzyme AGX1

A missense mutation in the catalytic domain of O-GlcNAc transferase links perturbations in protein O-GlcNAcylation to X-linked intellectual disability

A mouse model for functional dissection of TAB1 O-GlcNAcylation

A mutant O-GlcNAcase enriches Drosophila developmental regulators.

A structural and biochemical model of processive chitin synthesis

A structural snapshot of base-pair opening in DNA

An efficient synthesis of argifin: a natural product chitinase inhibitor with chemotherapeutic potential.

An intellectual disability syndrome with single-nucleotide variants in O-GlcNAc transferase

Analysis of the LKB1-STRAD-MO25 complex

Analyzing Airway Inflammation with Chemical Biology: Dissection of Acidic Mammalian Chitinase Function with a Selective Drug-like Inhibitor

Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein

Bisdionin C-a rationally designed, submicromolar inhibitor of family 18 chitinases

Bisubstrate UDP–peptide conjugates as human O-GlcNAc transferase inhibitors

BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm.

Catalytic deficiency of O-GlcNAc transferase leads to X-linked intellectual disability

Charge-Surrounded Pockets and Electrostatic Interactions with Small Ions Modulate the Activity of Retroviral Fusion Proteins ⬇️

Chemical Dissection of the Link between Streptozotocin, O-GlcNAc, and Pancreatic Cell Death

Chemical tools to probe cellular O-GlcNAc signalling.

Comparative structural analysis of retroviral fusion proteins identifies regions that modulate membrane fusion: a potential retroviral achilles heal?

Conformational substates in different crystal forms of the photoactive yellow protein-Correlation with theoretical and experimental flexibility

Crystal structure and binding properties of the Serratia marcescens chitin-binding protein CBP21.

Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 Å resolution 1 1Edited by R. Huber ⬇️

Crystal structures of allosamidin derivatives in complex with human macrophage chitinase

Crystallization and X-ray diffraction analysis of peroxisomal Δ3-Δ2-enoyl-CoA isomerase fromSaccharomyces cerevisiae

Crystallization and X-ray diffraction studies of the fatty-acid responsive transcription factor FadR from Escherichia coli

Discovery of catalytically active orthologues of the Parkinson's disease kinase PINK1: analysis of substrate specificity and impact of mutations.

Domain motions in bacteriophage T4 lysozyme: a comparison between molecular dynamics and crystallographic data.

Dual functionality of O-GlcNAc transferase is required for Drosophila development.

Dynamic properties of the guanine nucleotide binding protein alpha subunit and comparison of its guanosine triphosphate hydrolase domain with that of ras p21.

Efficient synthesis of 1,3,7-substituted xanthines by a safety-catch protection strategy

Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ESC differentiation without affecting naïve to primed cell transition.

Essential Dynamics from NMR Clusters: Dynamic Properties of the Myb DNA-Binding Domain and a Hinge-Bending Enhancing Variant ⬇️

Essential dynamics of DNA containing a cis.syn cyclobutane thymine dimer lesion.

Evidence for a Functional O-Linked N-Acetylglucosamine (O-GlcNAc) System in the Thermophilic Bacterium Thermobaculum terrenum

Functional concerted motions in the bovine serum retinol-binding protein

Genetic recoding to dissect the roles of site-specific protein O-GlcNAcylation

Genetic validation of Aspergillus fumigatus phosphoglucomutase as a viable therapeutic target in invasive aspergillosis

GlcNAcstatin: a picomolar, selective O-GlcNAcase inhibitor that modulates intracellular O-glcNAcylation levels

GlcNAcstatins are nanomolar inhibitors of human O -GlcNAcase inducing cellular hyper- O -GlcNAcylation

Glucose and glutamine fuel protein O-GlcNAcylation to control T cell self-renewal and malignancy

Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex

High resolution crystal structures of Siglec-7. Insights into ligand specificity in the Siglec family

High resolution crystal structures of unliganded and liganded human liver ACBP reveal a new mode of binding for the acyl-CoA ligand

Human OGA binds substrates in a conserved peptide recognition groove ⬇️

Human YKL-39 is a pseudo-chitinase with retained chitooligosaccharide-binding properties

Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone

Lead optimization of a pyrazole sulfonamide series of Trypanosoma brucei N-myristoyltransferase inhibitors: identification and evaluation of CNS penetrant compounds as potential treatments for stage 2 human African trypanosomiasis

Loss of CRMP2 O-GlcNAcylation leads to reduced novel object recognition performance in mice

Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases

Methylxanthine drugs are chitinase inhibitors: investigation of inhibition and binding modes.

Molecular Mechanisms of Yeast Cell Wall Glucan Remodeling

Molecular mechanisms of O-GlcNAcylation.

Molecular recognition: O-GlcNAc transfer: size matters.

Mutation of the PDK1 PH Domain Inhibits Protein Kinase B/Akt, Leading to Small Size and Insulin Resistance

Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase.

Mutations in N-acetylglucosamine (O-GlcNAc) transferase in patients with X-linked intellectual disability

N-myristoyltransferase inhibitors as new leads to treat sleeping sickness

N-myristoyltransferase is a cell wall target in Aspergillus fumigatus

Natural Product–Guided Discovery of a Fungal Chitinase Inhibitor

O-GlcNAcase contributes to cognitive function in Drosophila

O-GlcNAcase fragment discovery with fluorescence polarimetry.

O-GlcNAcase: promiscuous hexosaminidase or key regulator of O-GlcNAc signaling?

O-GlcNAcylation of TAB1 modulates TAK1-mediated cytokine release.

PRODRG: a tool for high-throughput crystallography of protein-ligand complexes

Phosphorylation of synaptic vesicle protein 2A at Thr84 by casein kinase 1 family kinases controls the specific retrieval of synaptotagmin-1.

Prediction of protein conformational freedom from distance constraints

Protein O-GlcNAcylation is required for fibroblast growth factor signaling in Drosophila.

Proteome wide purification and identification of O-GlcNAc-modified proteins using click chemistry and mass spectrometry

Pseudokinases-remnants of evolution or key allosteric regulators?

Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus

Purification, crystallization and preliminary X-ray diffraction of a proteolytic fragment of PDK1 containing the pleckstrin homology domain.

Putting glycobiology on a structural footing.

Recognition of a glycosylation substrate by the O-GlcNAc transferase TPR repeats

Screening-based discovery of drug-likeO-GlcNAcase inhibitor scaffolds ⬇️

Screening-based discovery ofAspergillus fumigatusplant-type chitinase inhibitors

Sequence, chromophore extraction and 3-D model of the photoactive yellow protein from Rhodobacter sphaeroides.

Solid-phase synthesis of cyclic peptide chitinase inhibitors: SAR of the argifin scaffold.

Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases.