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List of works by Yoshihiro Shimizu

70 S ribosomes bind to Shine-Dalgarno sequences without required dissociations.

scientific article

A novel screening system for self-mRNA targeting proteins.

scientific article

A point mutation in ribosomal protein L7/L12 reduces its ability to form a compact dimer structure and to assemble into the GTPase center.

scientific article published in April 2003

A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing.

scientific article published on 5 March 2018

ArfA Recruits RF2 into Stalled Ribosomes ⬇️

scientific article published on August 21, 2012

Biochemical aspects of bacterial strategies for handling the incomplete translation processes.

scientific article published on 10 April 2014

Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics.

scientific article published on 21 February 2018

Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation

scientific article published on 05 May 2020

Crystal structure analysis of the translation factor RF3 (release factor 3) ⬇️

scientific article published on September 6, 2012

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8

scientific article published on 27 February 2013

Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins.

scientific article

Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system

scientific article published on 25 October 2007

Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis system

scientific article

Esterification of Escherichia coli tRNAs with D-histidine and D-lysine by aminoacyl-tRNA synthetases.

scientific article published in May 2005

Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.

scientific article

G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System

scientific article published on 02 November 2018

High-resolution crystal structure of peptidyl-tRNA hydrolase from Thermus thermophilus

scientific article published on 27 December 2018

In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli

scientific article published on 25 March 2020

Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells

scientific article published on 05 January 2019

Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene.

scientific article published on 6 January 2016

Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins

scientific article published on 31 May 2016

Mg2+ dependence of 70 S ribosomal protein flexibility revealed by hydrogen/deuterium exchange and mass spectrometry.

scientific article published on 18 December 2009

Phosphorogenic and spontaneous formation of tris(bipyridine)ruthenium in peptide scaffolds

scientific article published on 19 February 2019

Protein synthesis by pure translation systems.

scientific article

Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase

scientific article

Quantitative Characterization of Translational Riboregulators Using an in Vitro Transcription-Translation System

scientific article published on 4 April 2018

Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling.

scientific article published on 06 February 2017

Real-time monitoring of cell-free translation on a quartz-crystal microbalance.

scientific article

Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs

scientific article published on 03 July 2020

Reconstitution of 30S ribosomal subunits in vitro using ribosome biogenesis factors

scientific article published on 03 August 2018

Ribosomal protein S1 is not essential for the trans-translation machinery.

scientific article published on 2 March 2007

Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational Modeling

scientific article published on 26 July 2018

Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome

scientific article published on 28 January 2011

Single-Cell Screening of Tamoxifen Abundance and Effect Using Mass Spectrometry and Raman-Spectroscopy

scientific article published on 05 February 2019

SmpB triggers GTP hydrolysis of elongation factor Tu on ribosomes by compensating for the lack of codon-anticodon interaction during trans-translation initiation

scientific article published on 06 April 2006

Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase

scientific article

The PURE system for protein production.

scientific article published in January 2014

The role of SmpB protein in trans-translation.

scientific article published in March 2002

Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

scientific article published on 27 April 2020

Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis

scientific article published on 17 April 2014

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