List of works - Carlos H.I. Ramos

1H, 15N and 13C resonance assignments of the J-domain of co-chaperone Sis1 from Saccharomyces cerevisiae

scientific article published on 30 April 2018

A spectroscopic analysis of the interaction between the human regulatory proteins RACK1 and Ki-1/57

scientific article published on 01 May 2006

A spectroscopic‐based laboratory experiment for protein conformational studies* ⬇️

scientific article published on January 1, 2004

Acyldepsipeptide Analogs Dysregulate Human Mitochondrial ClpP Protease Activity and Cause Apoptotic Cell Death

scientific article published on 28 June 2018

Amyloid fibril formation by circularly permuted and C-terminally deleted mutants

scientific article published on 12 February 2011

An overview of the role of molecular chaperones in protein homeostasis.

scientific article published on February 2011

Analysis of molecular targets of Mycobacterium tuberculosis by analytical ultracentrifugation.

scientific article published on January 2011

Are Hsp90 inhibitors good candidates against Covid-19?

scientific article published on 11 November 2020

Biochemical and biophysical characterization of small heat shock proteins from sugarcane. Involvement of a specific region located at the N-terminus with substrate specificity

scientific article published on 21 January 2007

Biophysical characterization of the recombinant merozoite surface protein-3 of Plasmodium vivax.

scientific article published on 7 April 2008

Central domain deletions affect the SAXS solution structure and function of yeast Hsp40 proteins Sis1 and Ydj1.

scientific article

Characterization of nucleotide-induced changes on the quaternary structure of human 70 kDa heat shock protein Hsp70.1 by analytical ultracentrifugation.

scientific article

Circular permutation and deletion studies of myoglobin indicate that the correct position of its N-terminus is required for native stability and solubility but not for native-like heme binding and folding.

scientific article published in March 2005

Cloning and characterization of three hypothetical secretion chaperone proteins from Xanthomonas axonopodis pv. citri.

scientific article published on 2 February 2007

Cloning, purification and characterization of a 90kDa heat shock protein from Citrus sinensis (sweet orange).

scientific article published on 16 August 2011

Comparative proteomics and metallomics studies in Arabidopsis thaliana leaf tissues: evaluation of the selenium addition in transgenic and nontransgenic plants using two-dimensional difference gel electrophoresis and laser ablation imaging.

scientific article

Conformational and functional studies of a cytosolic 90 kDa heat shock protein Hsp90 from sugarcane.

scientific article

Conformational changes in human Hsp70 induced by high hydrostatic pressure produce oligomers with ATPase activity but without chaperone activity.

scientific article published on 29 April 2014

Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones.

scientific article published on 14 August 2008

Cooperative substrate binding by a diguanylate cyclase

scientific article published on 20 November 2014

Corrigendum: αB-crystallin interacts with and prevents stress-activated proteolysis of focal adhesion kinase by calpain in cardiomyocytes.

scientific article

DNA and heparin chaperone the refolding of purified recombinant replication protein A subunit 1 from Leishmania amazonensis.

scientific article

Diffusive motions control the folding and unfolding kinetics of the apomyoglobin pH 4 molten globule intermediate

scientific article published on 17 March 2007

Disaggregases, molecular chaperones that resolubilize protein aggregates

scientific article (publication date: August 2015)

Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human alpha-tropomyosin

scientific article published on 01 October 2004

Expression and variability of molecular chaperones in the sugarcane expressome.

scientific article

Fast purification of the Apo form and of a non-binding heme mutant of recombinant sperm whale myoglobin.

scientific article published in March 2003

Free human mitochondrial GrpE is a symmetric dimer in solution.

scientific article

From Conformation to Interaction: Techniques to Explore the Hsp70/Hsp90 Network

scientific article published on 05 May 2015

Heat Shock Protein 90 kDa (Hsp90) Has a Second Functional Interaction Site with the Mitochondrial Import Receptor Tom70.

scientific article published on 8 July 2016

Heat causes oligomeric disassembly and increases the chaperone activity of small heat shock proteins from sugarcane.

scientific article published on 15 January 2010

Heparin-binding sites in granulocyte-macrophage colony-stimulating factor. Localization and regulation by histidine ionization.

scientific article published on 15 July 2005

Hsp78 (78 kDa Heat Shock Protein), a Representative AAA Family Member Found in the Mitochondrial Matrix of Saccharomyces cerevisiae.

scientific article published on 23 August 2017

Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain.

scientific article

Human hnRNP Q re-localizes to cytoplasmic granules upon PMA, thapsigargin, arsenite and heat-shock treatments

scientific article (publication date: April 2009)

Human mitochondrial Hsp70 (mortalin): shedding light on ATPase activity, interaction with adenosine nucleotides, solution structure and domain organization

scientific article

Identification of regions involved in substrate binding and dimer stabilization within the central domains of yeast Hsp40 Sis1.

scientific article

Identification of the flagellar chaperone FlgN in the phytopathogen Xanthomonas axonopodis pathovar citri by its interaction with hook-associated FlgK

scientific article published on 10 May 2007

Initial characterization of newly identified mitochondrial and chloroplast small HSPs from sugarcane shows that these chaperones have different oligomerization states and substrate specificities

scientific article published on 04 June 2018

Insights into the structure and function of the C-terminus of SGTs (small glutamine-rich TPR-containing proteins): A study of the Aedes aegypti homolog

scientific article published in 2021

Insights on the structure of amyloid fibrils from site-directed mutagenesis.

scientific article

Is there nascent structure in the intrinsically disordered region of troponin I?

scientific article published on 14 February 2011

Kinetic mechanism of the inhibition of human urinary kallikrein by basic pancreatic trypsin inhibitor

scientific article published on 01 May 1995

LaRbp38: a Leishmania amazonensis protein that binds nuclear and kinetoplast DNAs.

scientific article

LaTBP1: a Leishmania amazonensis DNA-binding protein that associates in vivo with telomeres and GT-rich DNA using a Myb-like domain.

scientific article published on 29 June 2007

Leishmania replication protein A-1 binds in vivo single-stranded telomeric DNA.

scientific article

Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures

scientific article

Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.

scientific article

Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras.

scientific article published in February 2005

Mapping subdomains in the C-terminal region of troponin I involved in its binding to troponin C and to thin filament

scientific article (publication date: 25 June 1999)

Molecular chaperones and protein quality control

scientific article published on 01 February 2011

Molecular dynamics and circular dichroism studies of human and rat C-peptides.

scientific article published on 5 June 2006

Mutant p53 aggregates into prion-like amyloid oligomers and fibrils: implications for cancer.

scientific article

New protein-protein interactions identified for the regulatory and structural components and substrates of the type III Secretion system of the phytopathogen Xanthomonas axonopodis Pathovar citri.

scientific article

On the difference in stability between horse and sperm whale myoglobins.

scientific article

On the quaternary structure of a C-type lectin from Bothrops jararacussu venom--BJ-32 (BjcuL).

scientific article published on 7 November 2008

Optimization of a Methodology for Quantification and Removal of Zinc Gives Insights Into the Effect of This Metal on the Stability and Function of the Zinc-Binding Co-chaperone Ydj1

scientific article published on 11 June 2019

Overexpression and Characterization of The C-Terminal Domain of Human Siva1, A Proapoptotic Factor and Cytoskeleton Binding Protein.

scientific article published on 26 October 2015

Phosphate closes the solution structure of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Mycobacterium tuberculosis

scientific article published on 13 June 2006

Protein cutoff scanning: A comparative analysis of cutoff dependent and cutoff free methods for prospecting contacts in proteins.

scientific article published in February 2009

Protein folding assisted by chaperones

scientific article

Proteomics: The clinical link

scientific article published on 23 February 2012

Resveratrol prevents p53 aggregation and in breast cancer cells

Revealing the interaction mode of the highly flexible Sorghum bicolor Hsp70/Hsp90 organizing protein (Hop): A conserved carboxylate clamp confers high affinity binding to Hsp90.

scientific article published on 6 February 2018

Sir2-Related Protein 1 from Leishmania amazonensis is a glycosylated NAD+-dependent deacetylase.

scientific article

Solution Conformation and Heparin-induced Dimerization of the Full-length Extracellular Domain of the Human Amyloid Precursor Protein

article

Solution NMR investigation on the structure and function of the isolated J-domain from Sis1: Evidence of transient inter-domain interactions in the full-length protein

scientific article published on 26 May 2019

Sorafenib as an Inhibitor of RUVBL2

scientific article published on 14 April 2020

Specification of Hsp70 function by Type I and Type II Hsp40.

scientific article published on January 2015

Specificity of native-like interhelical hydrophobic contacts in the apomyoglobin intermediate.

scientific article published on March 1999

Spectroscopic and thermodynamic measurements of nucleotide-induced changes in the human 70-kDa heat shock cognate protein

scientific article published on 05 June 2006

Stability and folding studies of the N-domain of troponin C. Evidence for the formation of an intermediate.

scientific article

Stoichiometry and thermodynamics of the interaction between the C-terminus of human 90kDa heat shock protein Hsp90 and the mitochondrial translocase of outer membrane Tom70

scientific article published on 14 July 2011

Structural and functional characterization of the chaperone Hsp70 from sugarcane. Insights into conformational changes during cycling from cross-linking/mass spectrometry assays

scientific article published on 12 February 2014

Structural and stability studies of the human mtHsp70-escort protein 1: an essential mortalin co-chaperone.

scientific article

Structural studies of prephenate dehydratase from Mycobacterium tuberculosis H37Rv by SAXS, ultracentrifugation, and computational analysis.

scientific article published in September 2008

Structure and activity analysis of two spider toxins that alter sodium channel inactivation kinetics.

scientific article published in April 2009

Structure of chorismate synthase from Mycobacterium tuberculosis.

scientific article

Studies on the effect of the J-domain on the substrate binding domain (SBD) of Hsp70 using a chimeric human J-SBD polypeptide

scientific article published on 17 November 2018

Sugarcane Hsp101 is a hexameric chaperone that binds nucleotides

scientific article published on 31 August 2011

Sulfate anion stabilization of native ribonuclease A both by anion binding and by the Hofmeister effect.

scientific article

The C-terminal region of the human p23 chaperone modulates its structure and function.

scientific article published on 10 November 2014

The HD-GYP domain of RpfG mediates a direct linkage between the Rpf quorum-sensing pathway and a subset of diguanylate cyclase proteins in the phytopathogen Xanthomonas axonopodis pv citri.

scientific article

The effect of celastrol, a triterpene with antitumorigenic activity, on conformational and functional aspects of the human 90kDa heat shock protein Hsp90α, a chaperone implicated in the stabilization of the tumor phenotype.

scientific article

The effect of regulatory Ca2+ on the in situ structures of troponin C and troponin I: a neutron scattering study

scientific article published on 01 August 1998

The molybdate-binding protein (ModA) of the plant pathogen Xanthomonas axonopodis pv. citri.

scientific article

The network interaction of the human cytosolic 90 kDa heat shock protein Hsp90: A target for cancer therapeutics.

scientific article published on 02 January 2012

The putative telomerase reverse transcriptase component of Leishmania amazonensis: gene cloning and characterization.

scientific article published on 14 January 2006

The stability of wild-type and deletion mutants of human C-terminus Hsp70-interacting protein (CHIP).

scientific article published in May 2013

Thermodynamic analysis of interactions of the Hsp90 with adenosine nucleotides: A comparative perspective

scientific article published on 20 February 2019

αB-crystallin interacts with and prevents stress-activated proteolysis of focal adhesion kinase by calpain in cardiomyocytes.

scientific article published on 16 October 2014

List of works by Carlos H.I. Ramos

1H, 15N and 13C resonance assignments of the J-domain of co-chaperone Sis1 from Saccharomyces cerevisiae

A spectroscopic analysis of the interaction between the human regulatory proteins RACK1 and Ki-1/57

A spectroscopic‐based laboratory experiment for protein conformational studies* ⬇️

Acyldepsipeptide Analogs Dysregulate Human Mitochondrial ClpP Protease Activity and Cause Apoptotic Cell Death

Amyloid fibril formation by circularly permuted and C-terminally deleted mutants

An overview of the role of molecular chaperones in protein homeostasis.

Analysis of molecular targets of Mycobacterium tuberculosis by analytical ultracentrifugation.

Are Hsp90 inhibitors good candidates against Covid-19?

Biochemical and biophysical characterization of small heat shock proteins from sugarcane. Involvement of a specific region located at the N-terminus with substrate specificity

Biophysical characterization of the recombinant merozoite surface protein-3 of Plasmodium vivax.

Central domain deletions affect the SAXS solution structure and function of yeast Hsp40 proteins Sis1 and Ydj1.

Characterization of nucleotide-induced changes on the quaternary structure of human 70 kDa heat shock protein Hsp70.1 by analytical ultracentrifugation.

Circular permutation and deletion studies of myoglobin indicate that the correct position of its N-terminus is required for native stability and solubility but not for native-like heme binding and folding.

Cloning and characterization of three hypothetical secretion chaperone proteins from Xanthomonas axonopodis pv. citri.

Cloning, purification and characterization of a 90kDa heat shock protein from Citrus sinensis (sweet orange).

Comparative proteomics and metallomics studies in Arabidopsis thaliana leaf tissues: evaluation of the selenium addition in transgenic and nontransgenic plants using two-dimensional difference gel electrophoresis and laser ablation imaging.

Conformational and functional studies of a cytosolic 90 kDa heat shock protein Hsp90 from sugarcane.

Conformational changes in human Hsp70 induced by high hydrostatic pressure produce oligomers with ATPase activity but without chaperone activity.

Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones.

Cooperative substrate binding by a diguanylate cyclase

Corrigendum: αB-crystallin interacts with and prevents stress-activated proteolysis of focal adhesion kinase by calpain in cardiomyocytes.

DNA and heparin chaperone the refolding of purified recombinant replication protein A subunit 1 from Leishmania amazonensis.

Diffusive motions control the folding and unfolding kinetics of the apomyoglobin pH 4 molten globule intermediate

Disaggregases, molecular chaperones that resolubilize protein aggregates

Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human alpha-tropomyosin

Expression and variability of molecular chaperones in the sugarcane expressome.

Fast purification of the Apo form and of a non-binding heme mutant of recombinant sperm whale myoglobin.

Free human mitochondrial GrpE is a symmetric dimer in solution.

From Conformation to Interaction: Techniques to Explore the Hsp70/Hsp90 Network

Heat Shock Protein 90 kDa (Hsp90) Has a Second Functional Interaction Site with the Mitochondrial Import Receptor Tom70.

Heat causes oligomeric disassembly and increases the chaperone activity of small heat shock proteins from sugarcane.

Heparin-binding sites in granulocyte-macrophage colony-stimulating factor. Localization and regulation by histidine ionization.

Hsp78 (78 kDa Heat Shock Protein), a Representative AAA Family Member Found in the Mitochondrial Matrix of Saccharomyces cerevisiae.

Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain.

Human hnRNP Q re-localizes to cytoplasmic granules upon PMA, thapsigargin, arsenite and heat-shock treatments

Human mitochondrial Hsp70 (mortalin): shedding light on ATPase activity, interaction with adenosine nucleotides, solution structure and domain organization

Identification of regions involved in substrate binding and dimer stabilization within the central domains of yeast Hsp40 Sis1.

Identification of the flagellar chaperone FlgN in the phytopathogen Xanthomonas axonopodis pathovar citri by its interaction with hook-associated FlgK

Initial characterization of newly identified mitochondrial and chloroplast small HSPs from sugarcane shows that these chaperones have different oligomerization states and substrate specificities

Insights into the structure and function of the C-terminus of SGTs (small glutamine-rich TPR-containing proteins): A study of the Aedes aegypti homolog

Insights on the structure of amyloid fibrils from site-directed mutagenesis.

Is there nascent structure in the intrinsically disordered region of troponin I?

Kinetic mechanism of the inhibition of human urinary kallikrein by basic pancreatic trypsin inhibitor

LaRbp38: a Leishmania amazonensis protein that binds nuclear and kinetoplast DNAs.

LaTBP1: a Leishmania amazonensis DNA-binding protein that associates in vivo with telomeres and GT-rich DNA using a Myb-like domain.

Leishmania replication protein A-1 binds in vivo single-stranded telomeric DNA.

Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures

Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.

Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras.

Mapping subdomains in the C-terminal region of troponin I involved in its binding to troponin C and to thin filament

Molecular chaperones and protein quality control

Molecular dynamics and circular dichroism studies of human and rat C-peptides.

Mutant p53 aggregates into prion-like amyloid oligomers and fibrils: implications for cancer.

New protein-protein interactions identified for the regulatory and structural components and substrates of the type III Secretion system of the phytopathogen Xanthomonas axonopodis Pathovar citri.

On the difference in stability between horse and sperm whale myoglobins.

On the quaternary structure of a C-type lectin from Bothrops jararacussu venom--BJ-32 (BjcuL).

Optimization of a Methodology for Quantification and Removal of Zinc Gives Insights Into the Effect of This Metal on the Stability and Function of the Zinc-Binding Co-chaperone Ydj1

Overexpression and Characterization of The C-Terminal Domain of Human Siva1, A Proapoptotic Factor and Cytoskeleton Binding Protein.

Phosphate closes the solution structure of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Mycobacterium tuberculosis

Protein cutoff scanning: A comparative analysis of cutoff dependent and cutoff free methods for prospecting contacts in proteins.

Protein folding assisted by chaperones

Proteomics: The clinical link

Resveratrol prevents p53 aggregation and in breast cancer cells

Revealing the interaction mode of the highly flexible Sorghum bicolor Hsp70/Hsp90 organizing protein (Hop): A conserved carboxylate clamp confers high affinity binding to Hsp90.

Sir2-Related Protein 1 from Leishmania amazonensis is a glycosylated NAD+-dependent deacetylase.

Solution Conformation and Heparin-induced Dimerization of the Full-length Extracellular Domain of the Human Amyloid Precursor Protein

Solution NMR investigation on the structure and function of the isolated J-domain from Sis1: Evidence of transient inter-domain interactions in the full-length protein

Sorafenib as an Inhibitor of RUVBL2

Specification of Hsp70 function by Type I and Type II Hsp40.

Specificity of native-like interhelical hydrophobic contacts in the apomyoglobin intermediate.

Spectroscopic and thermodynamic measurements of nucleotide-induced changes in the human 70-kDa heat shock cognate protein

Stability and folding studies of the N-domain of troponin C. Evidence for the formation of an intermediate.

Stoichiometry and thermodynamics of the interaction between the C-terminus of human 90kDa heat shock protein Hsp90 and the mitochondrial translocase of outer membrane Tom70

Structural and functional characterization of the chaperone Hsp70 from sugarcane. Insights into conformational changes during cycling from cross-linking/mass spectrometry assays

Structural and stability studies of the human mtHsp70-escort protein 1: an essential mortalin co-chaperone.

Structural studies of prephenate dehydratase from Mycobacterium tuberculosis H37Rv by SAXS, ultracentrifugation, and computational analysis.

Structure and activity analysis of two spider toxins that alter sodium channel inactivation kinetics.

Structure of chorismate synthase from Mycobacterium tuberculosis.

Studies on the effect of the J-domain on the substrate binding domain (SBD) of Hsp70 using a chimeric human J-SBD polypeptide