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List of works by José María Requena

A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum

scientific article published on 01 March 2003

A Proteogenomic Approach to Unravel New Proteins Encoded in the Leishmania donovani (HU3) Genome

scientific article published in 2024

A dataset of proteins associated with Trypanosoma cruzi LYT1 mRNAs

research article

A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi

scientific article published in February 1988

A postgenomic view of the heat shock proteins in kinetoplastids

scientific article published on 25 April 2007

A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei

scientific article

A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage.

scientific article published on 29 April 2016

A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes

scientific article published in January 1997

ARAMIS: From systematic errors of NGS long reads to accurate assemblies

research article

Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection

scientific article published on 01 June 2004

Alpha tubulin genes from Leishmania braziliensis: genomic organization, gene structure and insights on their expression

scientific article

Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major

scientific article published on 06 May 2019

Analysis of post-transcriptional regulation operating on transcription products of the tandemly linked Leishmania infantum hsp70 genes

scientific article published in February 1997

Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis.

scientific article

Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination

scientific article

Analysis of the antigenic properties of the L. infantum Hsp70: design of synthetic peptides for specific serodiagnosis of human leishmaniasis

article

Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis

scientific article published on 01 February 1999

Antigenic properties of the Leishmania infantum GRP94 and mapping of linear B-cell epitopes

scientific article published on 01 March 2002

Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis.

scientific article

Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis

scientific article

Assembly of a Large Collection of Maxicircle Sequences and Their Usefulness for Leishmania Taxonomy and Strain Typing

research article

Assignment of genes to Leishmania infantum chromosomes: karyotype and ploidy

scientific article published on 01 June 1995

Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells

scientific article published on 01 October 1993

Cell-cycle-dependent translation of histone mRNAs is the key control point for regulation of histone biosynthesis in Leishmania infantum

scientific article published on May 2004

Characterisation of a monoclonal antibody recognising specifically the HSP70 from Leishmania ⬇️

scientific article published on 01 November 2001

Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification

scientific article

Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3'-end of a poly(A)+ transcript.

scientific article published in September 1994

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi ⬇️

scientific article published on December 1, 1997

Characterization of the Immunostimulatory Properties ofLeishmania infantumHSP70 by Fusion to theEscherichia coliMaltose-Binding Protein in Normal andnu/nuBALB/c Mice ⬇️

scientific article published on January 1, 1998

Characterization of the antigenic determinants of the Leishmania infantum histone H3 recognized by antibodies elicited during canine visceral leishmaniasis

scientific article published on 01 December 1996

Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing

research article

Cloning of 20 kDa heat shock protein of Leishmania amazonensis

research article

Cloning of genes and expression and antigenicity analysis of the Leishmania infantum KMP-11 protein

scientific article published in January 1997

Cloning, sequencing, and expression of the PSA genes from Leishmania infantum

scientific article published in January 1998

Complete and de novo assembly of the Leishmania braziliensis (M2904) genome

article by Sandra González-de la Fuente et al published 10 December 2018 in Memórias do Instituto Oswaldo Cruz

Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation

scientific article published on 16 April 2019

Complete nucleotide sequence of the hsp70 gene of T. cruzi

scientific article published on January 1989

Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes

scientific article published on 01 January 2003

Contributions to the evolution and taxonomic classification of medical importance genus of the Trypanosomatidae family based on the phylogenetic analysis of the heat shock protein 70 gene

scientific article

Cytoplasmic-nuclear translocation of the Hsp70 protein during environmental stress in Trypanosoma cruzi

scientific article published on 01 November 1993

DNA binding properties and antileukemic (L1210) activity of a Pt-pentamidine complex

scientific article published on January 1991

Dataset for distribution of SIDER2 elements in the Leishmania major genome and transcriptome.

scientific article published on 10 January 2017

Datasets of Iso-Seq transcripts for decoding transcriptome complexity in four Leishmania species

scientific article published on November 2023

Detection of Leishmania spp. based on the gene encoding HSP20

scientific article published in October 2014

Dipeptidyl peptidase 3, a novel protease from Leishmania braziliensis.

scientific article published on 5 January 2018

Draft Genome Sequence of the Trypanosoma cruzi B. M. López Strain (TcIa), Isolated from a Colombian Patient

scientific article published on 30 April 2020

During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins

scientific article published on 01 May 1995

During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response

scientific article published on September 1996

During canine viscero-cutaneous leishmaniasis the anti-Hsp70 antibodies are specifically elicited by the parasite protein

scientific article published on March 1996

Effects of the disruption of the HSP70-II gene on the growth, morphology, and virulence of Leishmania infantum promastigotes.

scientific article

Efficient 5′-End Labeling of Oligonucleotides Containing Self-Complementary Sequences ⬇️

scientific article published on 01 October 1997

Evidence of RNA editing in Leishmania braziliensis promastigotes

scientific article published on 4 December 2010

Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene ⬇️

scientific article published on May 28, 2013

Evolutionarily conserved proteins as prominent immunogens during Leishmania infections

scientific article

Exploring the functionality and conservation of Alba proteins in Trypanosoma cruzi: A focus on biological diversity and RNA binding ability

scientific article published in June 2024

Gene annotation and transcriptome delineation on a de novo genome assembly for the reference Leishmania major friedlin strain

scientific article

Genes and Chromosomes of Leishmania infantum ⬇️

scientific article published on November 1, 1997

Genomic DNA macroarrays as a tool for analysis of gene expression in Leishmania

scientific article

Genomic and transcriptomic alterations in Leishmania donovani lines experimentally resistant to antileishmanial drugs

scientific article published on 13 April 2018

Genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the Leishmania major SIDER2 family and identification of SIDER2-containing transcripts.

scientific article published on 26 December 2016

Genomic clustering of the Trypanosoma cruzi nonlong terminal L1Tc retrotransposon with defined interspersed repeated DNA elements

scientific article published on 01 August 2000

Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species

scientific article published on 23 October 2006

Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania.

scientific article

Genomic repetitive DNA elements of Trypanosoma cruzi

scientific article published on 01 July 1996

Gimatecan and other camptothecin derivatives poison Leishmania DNA-topoisomerase IB leading to a strong leishmanicidal effect.

scientific article published in March 2013

High-efficiency plating method for Leishmania infantum

scientific article published in August 2003

Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control

scientific article published in February 2000

Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain

article

Identification of a putative regulatory element in the 3'-untranslated region that controls expression of HSP70 in Leishmania infantum

scientific article published on 01 September 2000

Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis.

scientific article published on 20 September 2013

Identification of regulatory proteins of gene expression in trypanosomatids

research article

Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization

scientific article

Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis

scientific article published on 01 November 1995

Identification of the interactomes associated with SCD6 and RBP42 proteins in Leishmania braziliensis

scientific article published on 06 December 2020

Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model

scientific article published on 01 October 2000

Immunity to visceral leishmaniasis

scientific article published on 28 December 2011

Immunization with Leishmania amazonensis subgenomic libraries protects BALB/c mice against the challenge

scientific article published in May 2004

Immunogenicity of HSP-70, KMP-11 and PFR-2 leishmanial antigens in the experimental model of canine visceral leishmaniasis

article

Immunohistological features of visceral leishmaniasis in BALB/c mice

scientific article published on 01 May 2006

Immunostimulatory properties of the Leishmania infantum heat shock proteins HSP70 and HSP83

scientific article published on 01 December 1999

Indotecan (LMP400) and AM13-55: two novel indenoisoquinolines show potential for treating visceral leishmaniasis

scientific article

Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

scientific article

Isolation of Trypanosoma cruzi specific nuclear repeated DNA sequences

scientific article published on 01 January 1993

Isolation, characterization and analysis of the expression of the Leishmania ribosomal P0 protein genes

scientific article

Leishmania Mitochondrial Genomes: Maxicircle Structure and Heterogeneity of Minicircles

scientific article published on 26 September 2019

Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity.

scientific article published on 19 December 2017

Leishmania braziliensis genome

Leishmania braziliensis (M2904) genome sequence

Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA.

scientific article published on 12 December 2014

Leishmania donovani genome assembly

Assembly and sequencing of L. donovani (HU3 strain) genome

Leishmania infantum (JPCM5) Transcriptome, Gene Models and Resources for an Active Curation of Gene Annotations

scientific article

Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation ⬇️

scientific article published on July 27, 2011

Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression

scientific article published in August 2003

Leishmania infantum transfected with toxic plasmid induces protection in mice infected with wild type L. infantum or L. amazonensis

scientific article published on 17 September 2020

Leishmania infantum: gene cloning of the GRP94 homologue, its expression as recombinant protein, and analysis of antigenicity

scientific article published in October 2000

Lights and shadows on gene organization and regulation of gene expression in Leishmania ⬇️

scientific article published on June 1, 2011

Live attenuated vaccines, a favorable strategy to provide long-term immunity against protozoan diseases

research article

Mapping of antigenic determinants of the T. cruzi hsp70 in chagasic and healthy individuals

scientific article published on 01 August 1993

Mapping of the antigenic determinants of the Leishmania infantum gp63 protein recognized by antibodies elicited during canine visceral leishmaniasis.

scientific article published on June 1997

Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis

scientific article published on 01 December 1995

Mapping of the linear antigenic determinants of the Leishmania infantum hsp70 recognized by leishmaniasis sera

scientific article published on 01 September 1996

Maxicircle genome partial sequence of leishmania braziliensis: Assembling and comparison with other trypanosomatids

scientific article wrote in English

Molecular Chaperones of Leishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes

scientific article published on 18 June 2015

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

scientific article published on 31 March 2020

Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani.

scientific article

Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes

scientific article published in December 1997

Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes

scientific article

Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi genome project

scientific article

More panantigens in Leishmania

scientific article

Multicomponent Chimeric Antigen for Serodiagnosis of Canine Visceral Leishmaniasis ⬇️

scientific article published on January 1, 1998

Omics approaches for understanding gene expression in Leishmania: clues for tackling leishmaniasis

Book chapter

Organization, transcription and regulation of the Leishmania infantum histone H3 genes

scientific article published on September 1996

Phage recovery by electroporation of naked DNA into host cells avoids the use of packaging extracts.

scientific article

Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania

scientific article published on 05 August 2007

Polymerase Chain Reaction Procedure to Detect Trypanosoma cruzi in Blood Samples from Chronic Chagasic Patients ⬇️

scientific article published on 01 March 1998

Pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis

scientific article published on 27 November 2017

Presence of antibodies in the aqueous humour and cerebrospinal fluid during Leishmania infections in dogs. Pathological features at the central nervous system ⬇️

scientific article published on November 1, 1996

Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant

research article

Proteins interacting with Leishmania major PUF1: A proteomic dataset.

scientific article

Protozoan parasites in the multi-omics era: present and future

scientific article published on 2023

Recent advances in vaccines for leishmaniasis

scientific article published in September 2004

Refinement of Leishmania donovani Genome Annotations in the Light of Ribosome-Protected mRNAs Fragments (Ribo-Seq Data)

scientific article published on 17 August 2023

Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase

scientific article published on 01 July 1992

Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome

scientific article published on 08 February 2016

Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs.

scientific article published on 22 December 2017

Sequence analysis of the 3’-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification ⬇️

scientific article published on April 28, 2012

Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA.

scientific article published in September 1990

Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.

scientific article

Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice

scientific article published on 23 March 2020

The Astonishing Large Family of HSP40/DnaJ Proteins Existing in Leishmania

research article

The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

scientific article published on 02 September 2020

The Leishmania HSP20 is antigenic during natural infections, but, as DNA vaccine, it does not protect BALB/c mice against experimental L. amazonensis infection

scientific article published on January 2008

The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis

scientific article

The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-gamma) production by murine splenocytes

scientific article published on 01 November 2000

The Leishmania infantum acidic ribosomal protein P0 administered as a DNA vaccine confers protective immunity to Leishmania major infection in BALB/c mice

scientific article

The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain

scientific article published on 01 October 1994

The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship

scientific article

The anti Z-DNA reactivity of Z-DNA forming sequences is affected by platinum antitumor drugs.

scientific article

The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation

scientific article

The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes of Drosophila hydei

scientific article published in September 1990

The heat shock proteins, Hsp70 and Hsp83, of Leishmania infantum are mitogens for mouse B cells

scientific article

The mRNA coding for the nucleosomal protein H2A ofLeishmaniais polyadenylated and has stem-loops at the 3′ end ⬇️

scientific article published on August 25, 1991

The transcriptome of Leishmania major in the axenic promastigote stage: transcript annotation and relative expression levels by RNA-seq.

scientific article

The translational efficiencies of the two Leishmania infantum HSP70 mRNAs, differing in their 3'-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms

scientific article published on 15 August 2005

Towards the physical map of the Trypanosoma cruzi nuclear genome: construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener

scientific article

Transcriptionally Driven DNA Replication Program of the Human Parasite Leishmania major

scientific article

Trypanosoma cruzi Ikiakarora (TcIII) Draft Genome Sequence

scientific article published on 02 July 2020

Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models

scientific article

Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis

scientific article published on 01 September 2004

Vaccine candidates against leishmania under current research.

scientific article

Variability of the Pr77 sequence of L1Tc retrotransposon among six T. cruzi strains belonging to different discrete typing units (DTUs)

scientific article

Virulence factors and immune evasion in Leishmania

Book chapter

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