List of works - Rafał Tomecki

2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion

2’- O -methylation of the second transcribed nucleotide within mRNA 5’ cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion

scientific article published on 3 February 2022

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

scientific article

An integrative approach uncovers transcriptome‐wide determinants of mRNA stability regulation in Saccharomyces cerevisiae

scientific article published in 2021

Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3'-terminal positions of synthesized tails

scientific article

Catalytic properties of the eukaryotic exosome

scientific article published on January 2011

Catalytic properties of the eukaryotic exosome.

scientific article published on January 2010

Comparison of preribosomal RNA processing pathways in yeast, plant and human cells - focus on coordinated action of endo- and exoribonucleases.

scientific article

Cytoplasmic RNA Decay

Cytoplasmic RNA decay pathways - Enzymes and mechanisms

scientific article

DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts

scientific article

Differential stability of mitochondrial mRNA in HeLa cells.

scientific article published on 21 December 2005

Elimination of 01/A'-A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3.

scientific article published on 28 September 2018

Endonucleolytic RNA cleavage by a eukaryotic exosome

scientific article

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA

scientific article

Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae

scientific article published on 16 August 2021

Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase

scientific article

Mechanisms of RNA degradation by the eukaryotic exosome

scientific article published on May 2010

Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target

scientific article published on 22 October 2013

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism

scientific article published on 30 July 2010

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs

scientific article published on 18 July 2016

Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by In Vitro Enzymatic Assays

RNA channelling by the eukaryotic exosome

scientific article

RNA degradation in yeast and human mitochondria

scientific article published in January 2004

Reproducible and efficient new method of RNA 3'-end labelling by CutA nucleotidyltransferase-mediated CC-tailing

scientific article published on 12 November 2021

Specific induction and carbon/nitrogen repression of arginine catabolism gene of Aspergillus nidulans--functional in vivo analysis of the otaA promoter

scientific article published in March 2003

Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine

scientific article published on 12 August 2020

Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family

scientific article

The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro

scientific article published on 12 February 2013

The RNA-Binding Landscape of HAX1 Protein Indicates Its Involvement in Translation and Ribosome Assembly

The eukaryotic RNA exosome: same scaffold but variable catalytic subunits

scientific article published on January 2011

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L

scientific article

Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines

scientific article published on 26 February 2006

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

scientific article published on 28 March 2018

hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0

scientific article

List of works by Rafał Tomecki

2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion

2’- O -methylation of the second transcribed nucleotide within mRNA 5’ cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

An integrative approach uncovers transcriptome‐wide determinants of mRNA stability regulation in Saccharomyces cerevisiae

Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3'-terminal positions of synthesized tails

Catalytic properties of the eukaryotic exosome

Catalytic properties of the eukaryotic exosome.

Comparison of preribosomal RNA processing pathways in yeast, plant and human cells - focus on coordinated action of endo- and exoribonucleases.

Cytoplasmic RNA Decay

Cytoplasmic RNA decay pathways - Enzymes and mechanisms

DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts

Differential stability of mitochondrial mRNA in HeLa cells.

Elimination of 01/A'-A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3.

Endonucleolytic RNA cleavage by a eukaryotic exosome

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA

Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae

Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase

Mechanisms of RNA degradation by the eukaryotic exosome

Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs

Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by In Vitro Enzymatic Assays

RNA channelling by the eukaryotic exosome

RNA degradation in yeast and human mitochondria

Reproducible and efficient new method of RNA 3'-end labelling by CutA nucleotidyltransferase-mediated CC-tailing

Specific induction and carbon/nitrogen repression of arginine catabolism gene of Aspergillus nidulans--functional in vivo analysis of the otaA promoter

Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine

Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family

The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro

The RNA-Binding Landscape of HAX1 Protein Indicates Its Involvement in Translation and Ribosome Assembly

The eukaryotic RNA exosome: same scaffold but variable catalytic subunits

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L

Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0