List of works - Krzysztof Rolka

A new proteinase 3 substrate with improved selectivity over human neutrophil elastase.

scientific article

A potentiometric and spectroscopic study of the proton and copper(II) complexes of methionine enkephalin and some related ligands

article published in 1985

A simple method for selection of trypsin chromogenic substrates using combinatorial chemistry approach.

scientific article

Activities of Venom Proteins and Peptides with Possible Therapeutic Applications from Bees and WASPS.

scientific article published on 18 June 2016

Analogues of Cucurbita maxima trypsin inhibitor III (CMTI-III) with elastase inhibitory activity

scientific article published on 01 April 1994

Analogues of Trypsin Inhibitor SFTI-1 with Disulfide Bridge Substituted by Various Length of Carbonyl Bridges

scientific article published on 01 October 2010

Analogues of trypsin inhibitor SFTI-1 modified in the conserved P₁' position by synthetic or non-proteinogenic amino acids retain their inhibitory activity

scientific article

Antibiotic-Based Conjugates Containing Antimicrobial HLopt2 Peptide: Design, Synthesis, Antimicrobial and Cytotoxic Activities

scientific article published on 25 September 2019

Antifungal action of human cathelicidin fragment (LL13-37) on Candida albicans

scientific article published on 26 August 2011

Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin

scientific article published on 10 August 2018

Atomic resolution crystal structure of HV-BBI protease inhibitor from amphibian skin in complex with bovine trypsin

scientific article

Biochemical and structural characterization of SplD protease from Staphylococcus aureus

scientific article

Bladder cancer detection using a peptide substrate of the 20S proteasome.

scientific article published on 21 June 2016

C-Terminal glycine is crucial for hyperalgesic activity of nociceptin/orphanin FQ-(1-6).

scientific article

Chemical synthesis and kinetic study of the smallest naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues

scientific article published on 01 April 2002

Chemical synthesis of new trypsin, chymotrypsin and elastase inhibitors by amino-acid substitutions in a trypsin inhibitor from squash seeds (CMTI III)

scientific article published on 01 January 1991

Chromogenic substrates of bovine beta-trypsin: the influence of an amino acid residue in P1 position on their interaction with the enzyme

scientific article published on 01 August 2001

Conformational solution studies of neuropeptide gamma using CD and NMR spectroscopy.

scientific article published in May 2002

Conformational studies of [Abu(3, 11)]-SFTI-1, an analogue of the trypsin inhibitor isolated from sunflower seeds.

scientific article

Conjugates of Ciprofloxacin and Levofloxacin with Cell-Penetrating Peptide Exhibit Antifungal Activity and Mammalian Cytotoxicity ⬇️

scientific article published on 30 June 2020

Convenient preparation of deuterium-labeled analogs of peptides containing N-substituted glycines for a stable isotope dilution LC-MS quantitative analysis.

scientific article

Copper(II) complexes with Fusobacterium nucleatum adhesin FadA: Coordination pattern, physicochemical properties and reactivity

scientific article published on 18 September 2018

Correction: Stability of Cu(II) complexes with FomA protein fragments containing two His residues in the peptide chain

scientific article published on 15 December 2020

Cu(II) complexes with peptides from FomA protein containing -His-Xaa-Yaa-Zaa-His and -His-His-motifs. ROS generation and DNA degradation

scientific article published on 06 September 2020

Cyclic Peptidic Furin Inhibitors Developed by Combinatorial Chemistry

scientific article published on 13 April 2023

Design and chemical syntheses of potent matriptase-2 inhibitors based on trypsin inhibitor SFTI-1 isolated from sunflower seeds.

scientific article published on 27 May 2017

Design and synthesis of new substrates of HtrA2 protease

scientific article published on 29 January 2015

Design of selective substrates of proteinase 3 using combinatorial chemistry methods

scientific article published on 07 April 2008

Design of serine proteinase inhibitors by combinatorial chemistry using trypsin inhibitor SFTI-1 as a starting structure.

scientific article

Design, chemical synthesis and kinetic studies of trypsin chromogenic substrates based on the proteinase binding loop of Cucurbita maxima trypsin inhibitor (CMTI-III)

scientific article published on 01 March 2000

Designing of substrates and inhibitors of bovine alpha-chymotrypsin with synthetic phenylalanine analogues in position P(1).

scientific article published in January 2008

Determination of nociceptin-orphanin FQ metabolites by capillary LC-MS

scientific article published on 01 November 2000

Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods.

scientific article

Distance between the basic group of the amino acid residue's side chain in position P1 of trypsin inhibitor CMTI-III and Asp189 in the substrate pocket of trypsin has an essential influence on the inhibitory activity

scientific article published on 01 November 1997

Dynorphin A inhibits nociceptin-converting enzyme from the rat spinal cord.

scientific article published in October 2001

Effect of scyliorhinin I and synthetic scyliorhinin I derivatives at mammalian tachykinin NK1, NK2 and NK3 receptors

scientific article published on 01 December 1993

Effects of cathelicidin and its fragments on three key enzymes of HIV-1.

scientific article published on 22 April 2011

Examples of peptide-peptoid hybrid serine protease inhibitors based on the trypsin inhibitor SFTI-1 with complete protease resistance at the P1-P1' reactive site

scientific article published in June 2005

Expanding chemical diversity of conotoxins: peptoid-peptide chimeras of the sodium channel blocker μ-KIIIA and its selenopeptide analogues

scientific article

Fluorescent analogs of trypsin inhibitor SFTI-1 isolated from sunflower seeds--synthesis and applications.

scientific article published on January 2014

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

article by Daniel J Klionsky et al published January 2021 in Autophagy

Highly specific substrates of proteinase 3 containing 3-(2-benzoxazol-5-yl)-l-alanine and their application for detection of this enzyme in human serum.

scientific article

Hybrid analogues of SFTI-1 modified in P1position by β- and γ-amino acids andN-substituted β-alanines

scientific article published on 01 April 2013

Identification of bikunin as an endogenous inhibitor of dynorphin convertase in human cerebrospinal fluid

scientific article published on 01 November 2006

Implication of the disulfide bridge in trypsin inhibitor SFTI-1 in its interaction with serine proteinases.

scientific article published on 29 October 2010

In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma

scientific article published in January 2003

Inhibition of human and yeast 20S proteasome by analogues of trypsin inhibitor SFTI-1

scientific article

Inhibitors of Matriptase-2 Based on the Trypsin Inhibitor SFTI-1

scientific article published on 19 June 2015

Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing

scientific article published on 19 April 2010

Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin

scientific article

Interactions between trypsin and its peptidic inhibitors studied by isothermal titration calorimetry (ITC)

Introduction of Pro and Its Analogues in the Conserved P1 Position of Trypsin Inhibitor SFTI-1 Retains Its Inhibitory Activity

scientific article published on 01 November 2011

Introduction of alpha-hydroxymethyamino acid residues in substrate specificity P1 position of trypsin inhibitor SFTI-1 from sunflower seeds retains its activity.

scientific article published on 20 December 2005

Introduction of non-natural amino acid residues into the substrate-specific P1 position of trypsin inhibitor SFTI-1 yields potent chymotrypsin and cathepsin G inhibitors.

scientific article published on 27 March 2009

Investigation of Serine-Proteinase-Catalyzed Peptide Splicing in Analogues of Sunflower Trypsin Inhibitor 1 (SFTI-1)

scientific article

Investigation of peptide splicing using two-peptide-chain analogs of trypsin inhibitor SFTI-1.

scientific article

Low-molecular-weight aldehyde inhibitors of cathepsin G.

scientific article

Matriptase-2: monitoring and inhibiting its proteolytic activity

Modifications outside the proteinase binding loop in Cucurbita maxima trypsin inhibitor III (CMTI-III) analogues change the binding energy with bovine beta-trypsin

scientific article published on 01 October 1998

Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials

scientific article published on 09 April 2020

New active analogues of Cucurbita maxima trypsin inhibitor III (CMTI-III) modified in the non-contact region

scientific article published on 01 January 1994

New chromogenic substrates of human neutrophil cathepsin G containing non-natural aromatic amino acid residues in position P(1) selected by combinatorial chemistry methods.

scientific article

New cyclosporin A analogue: synthesis and immunosuppressive activity

scientific article published on 01 September 1992

New potent cathepsin G phosphonate inhibitors

scientific article published on 31 August 2008

New ribosome-inactivating proteins and other proteins with protein synthesis-inhibiting activities

scientific article published on 19 March 2020

Nociceptin and its natural and specifically-modified fragments: Structural studies.

scientific article published on December 2010

Nociceptin inhibits acquisition of amphetamine-induced place preference and sensitization to stereotypy in rats.

scientific article published in August 2003

Noncovalent inhibitors of human 20S and 26S proteasome based on trypsin inhibitor SFTI-1.

scientific article published on 3 June 2016

Novel internally quenched substrate of the trypsin-like subunit of 20S eukaryotic proteasome

scientific article published on 24 August 2015

Orphanin FQ/nociceptin inhibits morphine withdrawal

scientific article

PEGylated substrates of NSP4 protease: A tool to study protease specificity.

scientific article

Pegylated fluorescent peptides as substrates of proteolytic enzymes

scientific article published on 01 December 2012

Peptide conjugates of lactoferricin analogues and antimicrobials-Design, chemical synthesis, and evaluation of antimicrobial activity and mammalian cytotoxicity

scientific article published on 05 April 2019

Peptide splicing in a double-sequence analogue of trypsin inhibitor SFTI-1 substituted in the P₁ positions by peptoid monomers.

scientific article published in May 2015

Peptomeric analogues of trypsin inhibitor SFTI-1 isolated from sunflower seeds

scientific article published in April 2008

Potentiometric and spectroscopic studies of the Cu(II) complexes of Ala-Arg8-vasopressin and oxytocin: two vasopressin-like peptides

scientific article published on 01 February 1992

Rat neuronal cells in primary culture as a model for nociceptin/orphanin FQ metabolism.

scientific article published in September 2003

Relationship between copper(ii) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

scientific article published on 01 April 2018

Selection of low-molecular-mass trypsin and chymotrypsin inhibitors based on the binding loop of CMTI-III using combinatorial chemistry methods.

scientific article published in October 2003

Selection of new chromogenic substrates of serine proteinases using combinatorial chemistry methods.

scientific article

Selection of peptomeric inhibitors of bovine alpha-chymotrypsin and cathepsin G based on trypsin inhibitor SFTI-1 using a combinatorial chemistry approach

scientific article

Selenopeptide analogs of EETI-II retain potent trypsin inhibitory activities.

scientific article

Solution conformational study of Scyliorhinin I analogues with conformational constraints by two-dimensional NMR and theoretical conformational analysis.

scientific article

Spliced analogues of trypsin inhibitor SFTI-1 and their application for tracing proteolysis and delivery of cargos inside the cells

scientific article published on 14 September 2016

Structural and Dynamic Characterization of Copper(II) Binding of the Human Prion Protein Outside the Octarepeat Region

scientific article published on 01 January 2007

Structure of a proteolytically resistant analogue of (NLys)5SFTI-1 in complex with trypsin: evidence for the direct participation of the Ser214 carbonyl group in serine protease-mediated proteolysis

scientific article

Substrate specificity and inhibitory study of human airway trypsin-like protease.

scientific article

Substrate specificity of Staphylococcus aureus cysteine proteases--Staphopains A, B and C.

scientific article

Substrate specificity of human matriptase-2.

scientific article

Sunflower trypsin inhibitor 1 as a molecular scaffold for drug discovery

scientific article published on December 2011

The antifungal properties of chicken egg cystatin against Candida yeast isolates showing different levels of azole resistance.

scientific article published on 22 June 2009

The dimeric and tetrameric octarepeat fragments of prion protein behave differently to its monomeric unit

scientific article published on 29 March 2004

The influence of substrate peptide length on human beta-tryptase specificity

scientific article

The new fluorogenic substrates of neutrophil proteinase 3 optimized in prime site region.

scientific article published on 13 January 2010

The prion peptide forms ion channels in planar lipid bilayers.

scientific article published in January 2003

Three Wavelength Substrate System of Neutrophil Serine Proteinases

scientific article published on 08 August 2012

Truncation of Huia versabilis Bowman-Birk inhibitor increases its selectivity, matriptase-1 inhibitory activity and proteolytic stability

scientific article published on 10 March 2020

Trypsin inhibitors from the garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) seeds: isolation, characterization and chemical synthesis

scientific article published on 03 May 2007

Tuber Lectins with Potentially Exploitable Bioactivities

scientific article published on 01 January 2018

Ultrasensitive internally quenched substrates of human cathepsin L.

scientific article published on 23 August 2014

List of works by Krzysztof Rolka

A new proteinase 3 substrate with improved selectivity over human neutrophil elastase.

A potentiometric and spectroscopic study of the proton and copper(II) complexes of methionine enkephalin and some related ligands

A simple method for selection of trypsin chromogenic substrates using combinatorial chemistry approach.

Activities of Venom Proteins and Peptides with Possible Therapeutic Applications from Bees and WASPS.

Analogues of Cucurbita maxima trypsin inhibitor III (CMTI-III) with elastase inhibitory activity

Analogues of Trypsin Inhibitor SFTI-1 with Disulfide Bridge Substituted by Various Length of Carbonyl Bridges

Analogues of trypsin inhibitor SFTI-1 modified in the conserved P₁' position by synthetic or non-proteinogenic amino acids retain their inhibitory activity

Antibiotic-Based Conjugates Containing Antimicrobial HLopt2 Peptide: Design, Synthesis, Antimicrobial and Cytotoxic Activities

Antifungal action of human cathelicidin fragment (LL13-37) on Candida albicans

Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin

Atomic resolution crystal structure of HV-BBI protease inhibitor from amphibian skin in complex with bovine trypsin

Biochemical and structural characterization of SplD protease from Staphylococcus aureus

Bladder cancer detection using a peptide substrate of the 20S proteasome.

C-Terminal glycine is crucial for hyperalgesic activity of nociceptin/orphanin FQ-(1-6).

Chemical synthesis and kinetic study of the smallest naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues

Chemical synthesis of new trypsin, chymotrypsin and elastase inhibitors by amino-acid substitutions in a trypsin inhibitor from squash seeds (CMTI III)

Chromogenic substrates of bovine beta-trypsin: the influence of an amino acid residue in P1 position on their interaction with the enzyme

Conformational solution studies of neuropeptide gamma using CD and NMR spectroscopy.

Conformational studies of [Abu(3, 11)]-SFTI-1, an analogue of the trypsin inhibitor isolated from sunflower seeds.

Conjugates of Ciprofloxacin and Levofloxacin with Cell-Penetrating Peptide Exhibit Antifungal Activity and Mammalian Cytotoxicity ⬇️

Convenient preparation of deuterium-labeled analogs of peptides containing N-substituted glycines for a stable isotope dilution LC-MS quantitative analysis.

Copper(II) complexes with Fusobacterium nucleatum adhesin FadA: Coordination pattern, physicochemical properties and reactivity

Correction: Stability of Cu(II) complexes with FomA protein fragments containing two His residues in the peptide chain

Cu(II) complexes with peptides from FomA protein containing -His-Xaa-Yaa-Zaa-His and -His-His-motifs. ROS generation and DNA degradation

Cyclic Peptidic Furin Inhibitors Developed by Combinatorial Chemistry

Design and chemical syntheses of potent matriptase-2 inhibitors based on trypsin inhibitor SFTI-1 isolated from sunflower seeds.

Design and synthesis of new substrates of HtrA2 protease

Design of selective substrates of proteinase 3 using combinatorial chemistry methods

Design of serine proteinase inhibitors by combinatorial chemistry using trypsin inhibitor SFTI-1 as a starting structure.

Design, chemical synthesis and kinetic studies of trypsin chromogenic substrates based on the proteinase binding loop of Cucurbita maxima trypsin inhibitor (CMTI-III)

Designing of substrates and inhibitors of bovine alpha-chymotrypsin with synthetic phenylalanine analogues in position P(1).

Determination of nociceptin-orphanin FQ metabolites by capillary LC-MS

Development of sensitive cathepsin G fluorogenic substrate using combinatorial chemistry methods.

Distance between the basic group of the amino acid residue's side chain in position P1 of trypsin inhibitor CMTI-III and Asp189 in the substrate pocket of trypsin has an essential influence on the inhibitory activity

Dynorphin A inhibits nociceptin-converting enzyme from the rat spinal cord.

Effect of scyliorhinin I and synthetic scyliorhinin I derivatives at mammalian tachykinin NK1, NK2 and NK3 receptors

Effects of cathelicidin and its fragments on three key enzymes of HIV-1.

Examples of peptide-peptoid hybrid serine protease inhibitors based on the trypsin inhibitor SFTI-1 with complete protease resistance at the P1-P1' reactive site

Expanding chemical diversity of conotoxins: peptoid-peptide chimeras of the sodium channel blocker μ-KIIIA and its selenopeptide analogues

Fluorescent analogs of trypsin inhibitor SFTI-1 isolated from sunflower seeds--synthesis and applications.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Highly specific substrates of proteinase 3 containing 3-(2-benzoxazol-5-yl)-l-alanine and their application for detection of this enzyme in human serum.

Hybrid analogues of SFTI-1 modified in P1position by β- and γ-amino acids andN-substituted β-alanines

Identification of bikunin as an endogenous inhibitor of dynorphin convertase in human cerebrospinal fluid

Implication of the disulfide bridge in trypsin inhibitor SFTI-1 in its interaction with serine proteinases.

In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma

Inhibition of human and yeast 20S proteasome by analogues of trypsin inhibitor SFTI-1

Inhibitors of Matriptase-2 Based on the Trypsin Inhibitor SFTI-1

Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing

Inhibitory and antimicrobial activities of OGTI and HV-BBI peptides, fragments and analogs derived from amphibian skin

Interactions between trypsin and its peptidic inhibitors studied by isothermal titration calorimetry (ITC)

Introduction of Pro and Its Analogues in the Conserved P1 Position of Trypsin Inhibitor SFTI-1 Retains Its Inhibitory Activity

Introduction of alpha-hydroxymethyamino acid residues in substrate specificity P1 position of trypsin inhibitor SFTI-1 from sunflower seeds retains its activity.

Introduction of non-natural amino acid residues into the substrate-specific P1 position of trypsin inhibitor SFTI-1 yields potent chymotrypsin and cathepsin G inhibitors.

Investigation of Serine-Proteinase-Catalyzed Peptide Splicing in Analogues of Sunflower Trypsin Inhibitor 1 (SFTI-1)

Investigation of peptide splicing using two-peptide-chain analogs of trypsin inhibitor SFTI-1.

Low-molecular-weight aldehyde inhibitors of cathepsin G.

Matriptase-2: monitoring and inhibiting its proteolytic activity

Modifications outside the proteinase binding loop in Cucurbita maxima trypsin inhibitor III (CMTI-III) analogues change the binding energy with bovine beta-trypsin

Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials

New active analogues of Cucurbita maxima trypsin inhibitor III (CMTI-III) modified in the non-contact region

New chromogenic substrates of human neutrophil cathepsin G containing non-natural aromatic amino acid residues in position P(1) selected by combinatorial chemistry methods.

New cyclosporin A analogue: synthesis and immunosuppressive activity

New potent cathepsin G phosphonate inhibitors

New ribosome-inactivating proteins and other proteins with protein synthesis-inhibiting activities

Nociceptin and its natural and specifically-modified fragments: Structural studies.

Nociceptin inhibits acquisition of amphetamine-induced place preference and sensitization to stereotypy in rats.

Noncovalent inhibitors of human 20S and 26S proteasome based on trypsin inhibitor SFTI-1.

Novel internally quenched substrate of the trypsin-like subunit of 20S eukaryotic proteasome

Orphanin FQ/nociceptin inhibits morphine withdrawal

PEGylated substrates of NSP4 protease: A tool to study protease specificity.

Pegylated fluorescent peptides as substrates of proteolytic enzymes

Peptide conjugates of lactoferricin analogues and antimicrobials-Design, chemical synthesis, and evaluation of antimicrobial activity and mammalian cytotoxicity

Peptide splicing in a double-sequence analogue of trypsin inhibitor SFTI-1 substituted in the P₁ positions by peptoid monomers.

Peptomeric analogues of trypsin inhibitor SFTI-1 isolated from sunflower seeds

Potentiometric and spectroscopic studies of the Cu(II) complexes of Ala-Arg8-vasopressin and oxytocin: two vasopressin-like peptides

Rat neuronal cells in primary culture as a model for nociceptin/orphanin FQ metabolism.

Relationship between copper(ii) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

Selection of low-molecular-mass trypsin and chymotrypsin inhibitors based on the binding loop of CMTI-III using combinatorial chemistry methods.