List of works - Sigrid De Keersmaecker

2D proteome analysis initiates new insights on the Salmonella Typhimurium LuxS protein

scientific article

A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors.

scientific article

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine

scientific article published on 05 August 2020

A combined approach to study the protein glycosylation potential of Lactobacillus rhamnosus GG (LGG)

scientific article published on 01 January 2012

A community effort towards a knowledge-base and mathematical model of the human pathogen Salmonella Typhimurium LT2

scientific article

A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay.

scientific article published on 28 November 2015

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

scientific article

A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment.

scientific article published on 6 February 2018

Adherence factors of Lactobacillus in the human gastrointestinal tract.

scientific article published on 19 September 2007

Assessment of the Feasibility of a Future Integrated Larger-Scale Epidemiological Study to Evaluate Health Risks of Air Pollution Episodes in Children

scientific article published on 13 July 2022

Characterization of MabA, a modulator of Lactobacillus rhamnosus GG adhesion and biofilm formation.

scientific article published on 9 April 2010

Chemical synthesis of (S)-4,5-dihydroxy-2,3-pentanedione, a bacterial signal molecule precursor, and validation of its activity in Salmonella typhimurium.

scientific article published on 24 March 2005

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

scientific article published on 09 March 2020

Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein

scientific article

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies

scientific article

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

scientific article

Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium

scientific article published in April 2005

Concise and diversity-oriented route toward polysubstituted 2-aminoimidazole alkaloids and their analogues.

scientific article published on December 2010

DISTILLER: a data integration framework to reveal condition dependency of complex regulons in Escherichia coli

scientific article

Delineation of the Salmonella enterica serovar Typhimurium HilA regulon through genome-wide location and transcript analysis

scientific article published on 04 May 2007

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

scientific article published on 20 February 2018

Detection of Plasmid-Mediated Colistin Resistance, mcr-1 and mcr-2 Genes, in Salmonella spp. Isolated from Food at Retail in Belgium from 2012 to 2015.

scientific article published on 28 November 2017

Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria

scientific article published on 01 September 2008

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination

scientific article

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

scientific article published on 17 July 2015

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

scientific article published on 10 June 2016

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination

scientific article published on 11 August 2020

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

scientific article

Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

scientific article published on 21 August 2015

Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine

scientific article published on 17 August 2010

Experimental approaches to identify small RNAs and their diverse roles in bacteria--what we have learnt in one decade of MicA research

scientific article

Exploiting the Advantages of Molecular Tools for the Monitoring of Fungal Indoor Air Contamination: First Detection of Exophiala jeanselmei in Indoor Air of Air-Conditioned Offices

scientific article published on 10 December 2019

FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts

article

FabR regulates Salmonella biofilm formation via its direct target FabB.

scientific article

Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins.

scientific article published on July 2006

Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG.

scientific article published on 13 April 2007

Functional characterization of a mucus-specific LPXTG surface adhesin from probiotic Lactobacillus rhamnosus GG.

scientific article

Gender-dependent association between exhaled nitric oxide and the CC16 38AA genotype in young school children

scientific article published on 06 August 2020

Gene expression analysis of monospecies Salmonella typhimurium biofilms using differential fluorescence induction.

scientific article published on 21 January 2011

Genetic and biochemical characterization of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG

scientific article

Genome Sequence of EU-Unauthorized Genetically Modified Bacillus subtilis Strain 2014-3557 Overproducing Riboflavin, Isolated from a Vitamin B2 80% Feed Additive

scientific article

Genome Sequence of the Salmonella enterica subsp. enterica Serovar Namur Strain 05-2929, Lacking the Salmonella Atypical Fimbrial Operon

scientific article published on 10 April 2014

Genome-specific higher-order background models to improve motif detection.

scientific article published in February 2003

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

scientific article

High-throughput screening in search of broad applicable, small molecule inhibitors of salmonella biofilm formation.

scientific article

How to deal with the upcoming challenges in GMO detection in food and feed

scientific article

Identification and characterization of 4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-5H-pyrimido[5,4-b]indole derivatives as Salmonella biofilm inhibitors.

scientific article

Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products.

scientific article published on September 2007

Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing

scientific article published on 27 April 2020

Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.

scientific article

Impact of lipoteichoic acid modification on the performance of the probiotic Lactobacillus rhamnosus GG in experimental colitis.

scientific article

In silico identification and experimental validation of PmrAB targets in Salmonella typhimurium by regulatory motif detection

scientific article

Influence of genetic background on transformation and expression of Green Fluorescent Protein in Actinobacillus actinomycetemcomitans.

scientific article published in October 2005

Interference with the quorum sensing systems of Salmonella enterica serovar typhimurium: possibilities and implications.

scientific article published in January 2007

Lessons from probiotic–host interaction studies in murine models of experimental colitis ⬇️

scientific article published on July 28, 2011

Let LuxS speak up in AI-2 signaling.

scientific article published on 3 February 2006

Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG.

scientific article published on 15 December 2012

Living on the edge: probiotic cell surface modification and its impact on microbe-host interactions

scientific article published on 01 January 2009

MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.

scientific article

Mechanisms of probiotic-host interaction with IBD as a case study: a role for exopolysaccharides?

scientific article

Microarray analysis and motif detection reveal new targets of the Salmonella enterica serovar Typhimurium HilA regulatory protein, including hilA itself.

scientific article

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

scientific article published on January 2017

More robust detection of motifs in coexpressed genes by using phylogenetic information.

scientific article

NGS for (Hemato-) Oncology in Belgium: Evaluation of Laboratory Performance and Feasibility of a National External Quality Assessment Program

scientific article published on 29 October 2020

Noninvasive integrative approach applied to children in the context of recent air pollution exposure demonstrates association between fractional exhaled nitric oxide (FeNO) and urinary CC16

scientific article published in 2023

Nurturing scientific mutualism: a report from the 'Young Microbiologists Mini-Symposium on microbe signalling, organisation and pathogenesis'.

scientific article published on 28 July 2009

Optimized MOL-PCR for Characterization of Microbial Pathogens.

scientific article published on 6 January 2016

Small molecules for interference with cell-cell-communication systems in Gram-negative bacteria.

scientific article

Statistical framework for detection of genetically modified organisms based on Next Generation Sequencing

scientific article published on 21 July 2015

Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study

scientific article published on 08 August 2020

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution

scientific article published on 06 November 2021

Stretching polysaccharides on live cells using single molecule force spectroscopy

scientific article published on 28 May 2009

Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid.

scientific article published in June 2006

Structure-activity relationship of 2-hydroxy-2-aryl-2,3-dihydro-imidazo[1,2-a]pyrimidinium salts and 2N-substituted 4(5)-aryl-2-amino-1H-imidazoles as inhibitors of biofilm formation by Salmonella Typhimurium and Pseudomonas aeruginosa.

scientific article

Structure-activity relationship of 4(5)-aryl-2-amino-1H-imidazoles, N1-substituted 2-aminoimidazoles and imidazo[1,2-a]pyrimidinium salts as inhibitors of biofilm formation by Salmonella typhimurium and Pseudomonas aeruginosa.

scientific article published on 20 December 2010

Synthesis of N-acyl homoserine lactone analogues reveals strong activators of SdiA, the Salmonella enterica serovar Typhimurium LuxR homologue.

scientific article

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country

scientific article published on 01 August 2020

The genetic structure of the Belgian population.

scientific article

The proteome of Salmonella Typhimurium grown under in vivo-mimicking conditions.

scientific article published in February 2009

The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation

scientific article

Towards understanding gene expression in multispecies biofilms containing Salmonella typhimurium

scientific article published on January 1, 2012

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection

scientific article published on 04 August 2020

Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

scientific article

Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods

scientific article published in 2021

Whole Genome Sequence Analysis of Salmonella Enteritidis PT4 Outbreaks from a National Reference Laboratory's Viewpoint.

scientific article published on 11 September 2015

List of works by Sigrid De Keersmaecker

2D proteome analysis initiates new insights on the Salmonella Typhimurium LuxS protein

A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors.

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine

A combined approach to study the protein glycosylation potential of Lactobacillus rhamnosus GG (LGG)

A community effort towards a knowledge-base and mathematical model of the human pathogen Salmonella Typhimurium LT2

A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay.

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment.

Adherence factors of Lactobacillus in the human gastrointestinal tract.

Assessment of the Feasibility of a Future Integrated Larger-Scale Epidemiological Study to Evaluate Health Risks of Air Pollution Episodes in Children

Characterization of MabA, a modulator of Lactobacillus rhamnosus GG adhesion and biofilm formation.

Chemical synthesis of (S)-4,5-dihydroxy-2,3-pentanedione, a bacterial signal molecule precursor, and validation of its activity in Salmonella typhimurium.

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium

Concise and diversity-oriented route toward polysubstituted 2-aminoimidazole alkaloids and their analogues.

DISTILLER: a data integration framework to reveal condition dependency of complex regulons in Escherichia coli

Delineation of the Salmonella enterica serovar Typhimurium HilA regulon through genome-wide location and transcript analysis

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

Detection of Plasmid-Mediated Colistin Resistance, mcr-1 and mcr-2 Genes, in Salmonella spp. Isolated from Food at Retail in Belgium from 2012 to 2015.

Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Exopolysaccharides of Lactobacillus rhamnosus GG form a protective shield against innate immune factors in the intestine

Experimental approaches to identify small RNAs and their diverse roles in bacteria--what we have learnt in one decade of MicA research

Exploiting the Advantages of Molecular Tools for the Monitoring of Fungal Indoor Air Contamination: First Detection of Exophiala jeanselmei in Indoor Air of Air-Conditioned Offices

FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts

FabR regulates Salmonella biofilm formation via its direct target FabB.

Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins.

Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG.

Functional characterization of a mucus-specific LPXTG surface adhesin from probiotic Lactobacillus rhamnosus GG.

Gender-dependent association between exhaled nitric oxide and the CC16 38AA genotype in young school children

Gene expression analysis of monospecies Salmonella typhimurium biofilms using differential fluorescence induction.

Genetic and biochemical characterization of the cell wall hydrolase activity of the major secreted protein of Lactobacillus rhamnosus GG

Genome Sequence of EU-Unauthorized Genetically Modified Bacillus subtilis Strain 2014-3557 Overproducing Riboflavin, Isolated from a Vitamin B2 80% Feed Additive

Genome Sequence of the Salmonella enterica subsp. enterica Serovar Namur Strain 05-2929, Lacking the Salmonella Atypical Fimbrial Operon

Genome-specific higher-order background models to improve motif detection.

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

High-throughput screening in search of broad applicable, small molecule inhibitors of salmonella biofilm formation.

How to deal with the upcoming challenges in GMO detection in food and feed

Identification and characterization of 4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-5H-pyrimido[5,4-b]indole derivatives as Salmonella biofilm inhibitors.

Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products.

Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing

Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.

Impact of lipoteichoic acid modification on the performance of the probiotic Lactobacillus rhamnosus GG in experimental colitis.

In silico identification and experimental validation of PmrAB targets in Salmonella typhimurium by regulatory motif detection

Influence of genetic background on transformation and expression of Green Fluorescent Protein in Actinobacillus actinomycetemcomitans.

Interference with the quorum sensing systems of Salmonella enterica serovar typhimurium: possibilities and implications.

Lessons from probiotic–host interaction studies in murine models of experimental colitis ⬇️

Let LuxS speak up in AI-2 signaling.

Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG.

Living on the edge: probiotic cell surface modification and its impact on microbe-host interactions

MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.

Mechanisms of probiotic-host interaction with IBD as a case study: a role for exopolysaccharides?

Microarray analysis and motif detection reveal new targets of the Salmonella enterica serovar Typhimurium HilA regulatory protein, including hilA itself.

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

More robust detection of motifs in coexpressed genes by using phylogenetic information.

NGS for (Hemato-) Oncology in Belgium: Evaluation of Laboratory Performance and Feasibility of a National External Quality Assessment Program

Noninvasive integrative approach applied to children in the context of recent air pollution exposure demonstrates association between fractional exhaled nitric oxide (FeNO) and urinary CC16

Nurturing scientific mutualism: a report from the 'Young Microbiologists Mini-Symposium on microbe signalling, organisation and pathogenesis'.

Optimized MOL-PCR for Characterization of Microbial Pathogens.

Small molecules for interference with cell-cell-communication systems in Gram-negative bacteria.

Statistical framework for detection of genetically modified organisms based on Next Generation Sequencing

Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution

Stretching polysaccharides on live cells using single molecule force spectroscopy

Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid.

Structure-activity relationship of 2-hydroxy-2-aryl-2,3-dihydro-imidazo[1,2-a]pyrimidinium salts and 2N-substituted 4(5)-aryl-2-amino-1H-imidazoles as inhibitors of biofilm formation by Salmonella Typhimurium and Pseudomonas aeruginosa.

Structure-activity relationship of 4(5)-aryl-2-amino-1H-imidazoles, N1-substituted 2-aminoimidazoles and imidazo[1,2-a]pyrimidinium salts as inhibitors of biofilm formation by Salmonella typhimurium and Pseudomonas aeruginosa.

Synthesis of N-acyl homoserine lactone analogues reveals strong activators of SdiA, the Salmonella enterica serovar Typhimurium LuxR homologue.

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country

The genetic structure of the Belgian population.

The proteome of Salmonella Typhimurium grown under in vivo-mimicking conditions.