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List of works by Radka Chaloupkova

Ancestral Haloalkane Dehalogenases Show Robustness and Unique Substrate Specificity.

scientific article published on 17 April 2017

Are time-dependent fluorescence shifts at the tunnel mouth of haloalkane dehalogenase enzymes dependent on the choice of the chromophore?

scientific article published on 20 June 2013

Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium ⬇️

scientific article published on May 11, 2012

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

scientific article

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

scientific article published on 05 June 2009

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

scientific article published on 11 July 2017

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

scientific article published in September 2003

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

scientific article

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

scientific article published on 26 December 2017

Construction of cryptogein mutants, a proteinaceous elicitor from Phytophthora, with altered abilities to induce a defense reaction in tobacco cells.

scientific article published in May 2005

Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5

scientific article published on 24 April 2019

Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands.

scientific article published on 22 January 2011

Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31

scientific article

Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.

scientific article published on 25 May 2013

Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17

scientific article published on 28 October 2019

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

scientific article published on 23 March 2017

DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin ⬇️

scientific article published on August 11, 2013

Elicitin–membrane interaction is driven by a positive charge on the protein surface: Role of Lys13 residue in lipids loading and resistance induction

scientific article published in March 2011

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

scientific article

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

scientific article published on 21 December 2015

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

scientific article

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

scientific article published on 3 November 2015

Functional analysis of the aglycone-binding site of the maize beta-glucosidase Zm-p60.1.

scientific article

Gram-scale production of recombinant microbial enzymes in shake flasks.

scientific article published on 5 December 2017

Haloalkane dehalogenases: biotechnological applications.

scientific article published on 11 September 2012

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

scientific article published on 25 February 2018

Instability restricts signaling of multiple fibroblast growth factors.

scientific article

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

scientific article

Lincomycin biosynthesis involves a tyrosine hydroxylating heme protein of an unusual enzyme family

scientific article

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

scientific article published on 2 October 2003

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus

scientific article

Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases

scientific article published on 21 March 2013

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

scientific article

Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance.

scientific article

Rad52 SUMOylation affects the efficiency of the DNA repair

scientific article

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

scientific article

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

scientific article

Site-Specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence

scientific article published on 09 April 2015

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

scientific article published on June 27, 2011

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

scientific article

Study of enzymatic reaction by electrophoretically mediated microanalysis in a partially filled capillary with indirect or direct detection.

scientific article published in January 2004

Study of substrate inhibition by electrophoretically mediated microanalysis in partially filled capillary

scientific article published on 29 September 2006

Suppression of protein inactivation during freezing by minimizing pH changes using ionic cryoprotectants

scientific article published on 17 May 2016

The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice

scientific article published on 5 February 2016

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.

scientific article

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