List of works - Tomáš Obšil

14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.

scientific article published on 4 September 2004

14-3-3 protein binding blocks the dimerization interface of caspase-2

scientific article published on 21 January 2020

14-3-3 protein directly interacts with the kinase domain of calcium/calmodulin-dependent protein kinase kinase (CaMKK2).

scientific article published on 9 April 2018

14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G protein signaling 3 (RGS3)

scientific article

14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4.

scientific article

14-3-3 protein masks the nuclear localization sequence of caspase-2

scientific article published on 15 October 2018

14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites

scientific article published on 19 August 2021

14-3-3 proteins – A family of universal scaffolds and regulators ⬇️

scientific article published on September 29, 2011

14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains

scientific article published on 22 July 2021

14-3-3zeta C-terminal stretch changes its conformation upon ligand binding and phosphorylation at Thr232.

scientific article published on 12 November 2003

A Structural Study of the Cytoplasmic Chaperone Effect of 14-3-3 Proteins on Ataxin-1

scientific article published on 21 July 2021

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

scientific article published on 21 May 2015

A new role for 14-3-3 protein in steroidogenesis

scientific article published on 16 August 2020

ATP binding site on the C-terminus of the vanilloid receptor

scientific article published on 21 July 2007

Activation and Regulation of Purinergic P2X Receptor Channels ⬇️

scientific article published on July 7, 2011

Allosteric activation of yeast enzyme neutral trehalase by calcium and 14-3-3 protein

scientific article published on 10 January 2019

Biophysical and structural characterization of the thioredoxin-binding domain of protein kinase ASK1 and its interaction with reduced thioredoxin

scientific article published on 17 July 2014

Both the N-terminal loop and wing W2 of the forkhead domain of transcription factor Foxo4 are important for DNA binding

scientific article

CaMKK2 kinase domain interacts with the autoinhibitory region through the N-terminal lobe including the RP insert

scientific article published on 24 July 2018

Cysteine residues mediate high-affinity binding of thioredoxin to ASK1.

scientific article

Detailed kinetic analysis of the interaction between the FOXO4-DNA-binding domain and DNA.

scientific article published on 15 September 2013

Different cation binding to the I domains of α1 and α2 integrins: implication of the binding site structure

scientific article published on 01 September 1999

Effect of aminophospholipid glycation on order parameter and hydration of phospholipid bilayer

scientific article published on 01 August 1999

Effects of fluorescent pseudo-ATP and ATP-metal analogs on secondary structure of Na(+)/K(+)-ATPase

scientific article published on 01 February 2000

Erythrosin 5'-isothiocyanate labels Cys549 as part of the low-affinity ATP binding site of Na+/K+-ATPase

scientific article published on 01 December 1998

FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA

scientific article published in May 2022

Forkhead Domains of FOXO Transcription Factors Differ in both Overall Conformation and Dynamics

scientific article published on 24 August 2019

Functional characterization of mutants in the transmembrane domains of the rat P2X7 receptor that regulate pore conductivity and agonist sensitivity.

scientific article published on 18 March 2015

Hippo/Mst1 stimulates transcription of the proapoptotic mediator NOXA in a FoxO1-dependent manner

scientific article published on 18 January 2011

Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding

scientific article

Lengthening the Guanidine-Aryl Linker of Phenylpyrimidinylguanidines Increases Their Potency as Inhibitors of FOXO3-Induced Gene Transcription

scientific article published on 27 September 2022

Ligand binding to the human MT2 melatonin receptor: the role of residues in transmembrane domains 3, 6, and 7.

scientific article

Microenvironment of the high affinity ATP-binding site of Na+/K+-ATPase is slightly acidic

scientific article published on 01 January 1999

Modulating FOXO3 transcriptional activity by small, DBD-binding molecules

scientific article published on 4 December 2019

Modulators of 14-3-3 Protein-Protein Interactions ⬇️

scientific article

Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.

scientific article published on 30 October 2017

Molecular distance measurements reveal an (alpha beta)2 dimeric structure of Na+/K+-ATPase. High affinity ATP binding site and K+-activated phosphatase reside on different alpha-subunits

scientific article published on 01 October 1998

Molecular modeling of human MT2 melatonin receptor: the role of Val204, Leu272 and Tyr298 in ligand binding.

scientific article

Participation of the Lys313-Ile333 sequence of the purinergic P2X4 receptor in agonist binding and transduction of signals to the channel gate

scientific article published on 5 September 2006

Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1.

scientific article

Role of the EF-hand-like motif in the 14-3-3 protein-mediated activation of yeast neutral trehalase Nth1.

scientific article

Set-up and screening of a fragment library targeting the 14-3-3 protein interface

scientific article published on 22 July 2019

Stabilization of Protein-Protein Interactions between CaMKK2 and 14-3-3 by Fusicoccins

scientific article published on 04 November 2020

Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function

scientific article published in April 2017

Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.

scientific article published on 13 May 2015

Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1).

scientific article published on 11 August 2016

Structural Insights into the Function of P2X4: An ATP-Gated Cation Channel of Neuroendocrine Cells ⬇️

scientific article published on November 25, 2010

Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding ⬇️

scientific article published on November 7, 2012

Structural aspects of protein kinase ASK1 regulation.

scientific article

Structural basis of 14-3-3 protein functions.

scientific article published on 06 September 2011

Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.

scientific article published on 29 May 2013

Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA

scientific article

The 14-3-3 Proteins as Important Allosteric Regulators of Protein Kinases

scientific article published on 21 November 2020

The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase

scientific article

The C-terminal segment of yeast BMH proteins exhibits different structure compared to other 14-3-3 protein isoforms

scientific article published on 01 May 2010

The activity of Saccharomyces cerevisiae Na+, K+/H+ antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481

scientific article published on 22 August 2019

The interactions of the C-terminal region of the TRPC6 channel with calmodulin.

scientific article

The isolated H4-H5 cytoplasmic loop of Na,K-ATPase overexpressed in Escherichia coli retains its ability to bind ATP

scientific article published on 01 April 1998

The redox-active site of thioredoxin is directly involved in apoptosis signal-regulating kinase 1 binding that is modulated by oxidative stress

scientific article published on 17 October 2019

Two 14-3-3 binding motifs are required for stable association of Forkhead transcription factor FOXO4 with 14-3-3 proteins and inhibition of DNA binding.

scientific article published in December 2003

List of works by Tomáš Obšil

14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.

14-3-3 protein binding blocks the dimerization interface of caspase-2

14-3-3 protein directly interacts with the kinase domain of calcium/calmodulin-dependent protein kinase kinase (CaMKK2).

14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G protein signaling 3 (RGS3)

14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4.

14-3-3 protein masks the nuclear localization sequence of caspase-2

14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites

14-3-3 proteins – A family of universal scaffolds and regulators ⬇️

14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains

14-3-3zeta C-terminal stretch changes its conformation upon ligand binding and phosphorylation at Thr232.

A Structural Study of the Cytoplasmic Chaperone Effect of 14-3-3 Proteins on Ataxin-1

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

A new role for 14-3-3 protein in steroidogenesis

ATP binding site on the C-terminus of the vanilloid receptor

Activation and Regulation of Purinergic P2X Receptor Channels ⬇️

Allosteric activation of yeast enzyme neutral trehalase by calcium and 14-3-3 protein

Biophysical and structural characterization of the thioredoxin-binding domain of protein kinase ASK1 and its interaction with reduced thioredoxin

Both the N-terminal loop and wing W2 of the forkhead domain of transcription factor Foxo4 are important for DNA binding

CaMKK2 kinase domain interacts with the autoinhibitory region through the N-terminal lobe including the RP insert

Cysteine residues mediate high-affinity binding of thioredoxin to ASK1.

Detailed kinetic analysis of the interaction between the FOXO4-DNA-binding domain and DNA.

Different cation binding to the I domains of α1 and α2 integrins: implication of the binding site structure

Effect of aminophospholipid glycation on order parameter and hydration of phospholipid bilayer

Effects of fluorescent pseudo-ATP and ATP-metal analogs on secondary structure of Na(+)/K(+)-ATPase

Erythrosin 5'-isothiocyanate labels Cys549 as part of the low-affinity ATP binding site of Na+/K+-ATPase

FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA

Forkhead Domains of FOXO Transcription Factors Differ in both Overall Conformation and Dynamics

Functional characterization of mutants in the transmembrane domains of the rat P2X7 receptor that regulate pore conductivity and agonist sensitivity.

Hippo/Mst1 stimulates transcription of the proapoptotic mediator NOXA in a FoxO1-dependent manner

Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding

Lengthening the Guanidine-Aryl Linker of Phenylpyrimidinylguanidines Increases Their Potency as Inhibitors of FOXO3-Induced Gene Transcription

Ligand binding to the human MT2 melatonin receptor: the role of residues in transmembrane domains 3, 6, and 7.

Microenvironment of the high affinity ATP-binding site of Na+/K+-ATPase is slightly acidic

Modulating FOXO3 transcriptional activity by small, DBD-binding molecules

Modulators of 14-3-3 Protein-Protein Interactions ⬇️

Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.

Molecular distance measurements reveal an (alpha beta)2 dimeric structure of Na+/K+-ATPase. High affinity ATP binding site and K+-activated phosphatase reside on different alpha-subunits

Molecular modeling of human MT2 melatonin receptor: the role of Val204, Leu272 and Tyr298 in ligand binding.

Participation of the Lys313-Ile333 sequence of the purinergic P2X4 receptor in agonist binding and transduction of signals to the channel gate

Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1.

Role of the EF-hand-like motif in the 14-3-3 protein-mediated activation of yeast neutral trehalase Nth1.

Set-up and screening of a fragment library targeting the 14-3-3 protein interface

Stabilization of Protein-Protein Interactions between CaMKK2 and 14-3-3 by Fusicoccins

Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function

Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.

Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1).

Structural Insights into the Function of P2X4: An ATP-Gated Cation Channel of Neuroendocrine Cells ⬇️

Structural Modulation of Phosducin by Phosphorylation and 14-3-3 Protein Binding ⬇️

Structural aspects of protein kinase ASK1 regulation.

Structural basis of 14-3-3 protein functions.

Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.

Structure of the human FOXO4-DBD-DNA complex at 1.9 Å resolution reveals new details of FOXO binding to the DNA

The 14-3-3 Proteins as Important Allosteric Regulators of Protein Kinases

The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase

The C-terminal segment of yeast BMH proteins exhibits different structure compared to other 14-3-3 protein isoforms

The activity of Saccharomyces cerevisiae Na+, K+/H+ antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481

The interactions of the C-terminal region of the TRPC6 channel with calmodulin.

The isolated H4-H5 cytoplasmic loop of Na,K-ATPase overexpressed in Escherichia coli retains its ability to bind ATP

The redox-active site of thioredoxin is directly involved in apoptosis signal-regulating kinase 1 binding that is modulated by oxidative stress

Two 14-3-3 binding motifs are required for stable association of Forkhead transcription factor FOXO4 with 14-3-3 proteins and inhibition of DNA binding.