List of works - Siew Pheng Lim

2'-O methylation of internal adenosine by flavivirus NS5 methyltransferase

scientific article

A Conserved Pocket in the Dengue Virus Polymerase Identified through Fragment-based Screening

scientific article

A Crystal Structure of the Dengue Virus Non-structural Protein 5 (NS5) Polymerase Delineates Interdomain Amino Acid Residues That Enhance Its Thermostability and de Novo Initiation Activities

scientific article

A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication

scientific article

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease.

scientific article

A novel extracellular domain variant of the human integrin alpha 7 subunit generated by alternative intron splicing

scientific article

A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses.

scientific article published on 20 September 2008

Acquisition of immunogenicity by AKR leukemic cells following DNA-mediated gene transfer is associated with the reduction of constitutive reactive superoxide radicals

article

Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravir

scientific article

An anti-HIV-1 gp120 antibody expressed as an endocytotic transmembrane protein mediates internalization of HIV-1.

scientific article published on October 2003

Antiviral Nucleotide Incorporation by Recombinant Human Mitochondrial RNA Polymerase Is Predictive of Increased In Vivo Mitochondrial Toxicity Risk.

scientific article published on 19 September 2016

Arylalkylidene rhodanine with bulky and hydrophobic functional group as selective HCV NS3 protease inhibitor.

scientific article

Biochemical and genetic characterization of dengue virus methyltransferase

scientific article

CD81 engineered with endocytotic signals mediates HCV cell entry: implications for receptor usage by HCV in vivo

scientific article published on April 2003

Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

scientific article published in January 2007

Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication

scientific article

Characterization of a novel IRF-1-deficient mutant cell line

scientific article published on January 1994

Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor

scientific article

Crystal Structure of the Dengue Virus Methyltransferase Bound to a 5′-Capped Octameric RNA

scientific article

Dengue drug discovery: Progress, challenges and outlook

scientific article published on 29 December 2018

Dengue protease activity: the structural integrity and interaction of NS2B with NS3 protease and its potential as a drug target

scientific article published on October 2011

Detection and quantification of flavivirus NS5 methyl-transferase activities

scientific article published on January 2013

Differential polarization of immune responses by co-administration of antigens with chemokines.

scientific article published in December 2004

Discovery of Potent Non-Nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from a Fragment Hit Using Structure-Based Drug Design

scientific article

Discovery of Potent Non-nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from Fragment Screening and Structure-Guided Design

Flavivirus RNA methylation

scientific article published on 31 January 2014

Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication

scientific article

Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries.

scientific article

Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus

scientific article published on 29 March 2010

Identification and molecular characterisation of the complete genome of a Singapore isolate of hepatitis C virus: sequence comparison with other strains and phylogenetic analysis

scientific article published on January 2001

Identifying initiation and elongation inhibitors of dengue virus RNA polymerase in a high-throughput lead-finding campaign

scientific article

Inducible system in human hepatoma cell lines for hepatitis C virus production

scientific article published in November 2002

Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein

scientific article

Molecular basis for specific viral RNA recognition and 2'-O-ribose methylation by the dengue virus nonstructural protein 5 (NS5)

scientific article

NMR Analysis of the Dynamic Exchange of the NS2B Cofactor between Open and Closed Conformations of the West Nile Virus NS2B-NS3 Protease

scientific article

NS5 from Dengue Virus Serotype 2 Can Adopt a Conformation Analogous to That of Its Zika Virus and Japanese Encephalitis Virus Homologues

scientific article published on 12 December 2019

Novel inhibitors of dengue virus methyltransferase: discovery by in vitro-driven virtual screening on a desktop computer grid

scientific article

Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors.

scientific article published on 21 October 2005

Polymerases of hepatitis C viruses and flaviviruses: structural and mechanistic insights and drug development

scientific article published on 19 February 2014

Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling

scientific article

Purification and crystallization of dengue and West Nile virus NS2B–NS3 complexes

scientific article

Small Molecule Inhibitors That Selectively Block Dengue Virus Methyltransferase

scientific article

Stabilization of dengue virus polymerase in de novo initiation assay provides advantages for compound screening

scientific article published on 18 April 2015

Strategies for development of Dengue virus inhibitors

scientific article

Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus.

scientific article

Structure-activity relationship of uridine-based nucleoside phosphoramidate prodrugs for inhibition of dengue virus RNA-dependent RNA polymerase

scholarly article by Gang Wang et al published 15 July 2018 in Bioorganic & Medicinal Chemistry Letters

Ten years of dengue drug discovery: progress and prospects

scientific article published on 27 September 2013

The Dengue Virus Replication Complex: From RNA Replication to Protein-Protein Interactions to Evasion of Innate Immunity.

scientific article published in January 2018

The dengue virus NS5 protein as a target for drug discovery

scientific article published on 24 April 2015

The human immunodeficiency virus type 1 Tat protein up-regulates the promoter activity of the beta-chemokine monocyte chemoattractant protein 1 in the human astrocytoma cell line U-87 MG: role of SP-1, AP-1, and NF-kappaB consensus sites

scientific article

Towards the design of antiviral inhibitors against flaviviruses: the case for the multifunctional NS3 protein from Dengue virus as a target

scientific article published on 30 July 2008

Two RNA tunnel inhibitors bind in highly conserved sites in Dengue virus NS5 polymerase: structural and functional studies

scientific article published on 09 September 2020

West Nile virus drug discovery

scientific article published on 03 December 2013

Yellow fever virus NS3 protease: peptide-inhibition studies.

scientific article published on August 2007

List of works by Siew Pheng Lim

2'-O methylation of internal adenosine by flavivirus NS5 methyltransferase

A Conserved Pocket in the Dengue Virus Polymerase Identified through Fragment-based Screening

A Crystal Structure of the Dengue Virus Non-structural Protein 5 (NS5) Polymerase Delineates Interdomain Amino Acid Residues That Enhance Its Thermostability and de Novo Initiation Activities

A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease.

A novel extracellular domain variant of the human integrin alpha 7 subunit generated by alternative intron splicing

A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses.

Acquisition of immunogenicity by AKR leukemic cells following DNA-mediated gene transfer is associated with the reduction of constitutive reactive superoxide radicals

Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravir

An anti-HIV-1 gp120 antibody expressed as an endocytotic transmembrane protein mediates internalization of HIV-1.

Antiviral Nucleotide Incorporation by Recombinant Human Mitochondrial RNA Polymerase Is Predictive of Increased In Vivo Mitochondrial Toxicity Risk.

Arylalkylidene rhodanine with bulky and hydrophobic functional group as selective HCV NS3 protease inhibitor.

Biochemical and genetic characterization of dengue virus methyltransferase

CD81 engineered with endocytotic signals mediates HCV cell entry: implications for receptor usage by HCV in vivo

Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication

Characterization of a novel IRF-1-deficient mutant cell line

Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor

Crystal Structure of the Dengue Virus Methyltransferase Bound to a 5′-Capped Octameric RNA

Dengue drug discovery: Progress, challenges and outlook

Dengue protease activity: the structural integrity and interaction of NS2B with NS3 protease and its potential as a drug target

Detection and quantification of flavivirus NS5 methyl-transferase activities

Differential polarization of immune responses by co-administration of antigens with chemokines.

Discovery of Potent Non-Nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from a Fragment Hit Using Structure-Based Drug Design

Discovery of Potent Non-nucleoside Inhibitors of Dengue Viral RNA-Dependent RNA Polymerase from Fragment Screening and Structure-Guided Design

Flavivirus RNA methylation

Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication

Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries.

Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus

Identification and molecular characterisation of the complete genome of a Singapore isolate of hepatitis C virus: sequence comparison with other strains and phylogenetic analysis

Identifying initiation and elongation inhibitors of dengue virus RNA polymerase in a high-throughput lead-finding campaign

Inducible system in human hepatoma cell lines for hepatitis C virus production

Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein

Molecular basis for specific viral RNA recognition and 2'-O-ribose methylation by the dengue virus nonstructural protein 5 (NS5)

NMR Analysis of the Dynamic Exchange of the NS2B Cofactor between Open and Closed Conformations of the West Nile Virus NS2B-NS3 Protease

NS5 from Dengue Virus Serotype 2 Can Adopt a Conformation Analogous to That of Its Zika Virus and Japanese Encephalitis Virus Homologues

Novel inhibitors of dengue virus methyltransferase: discovery by in vitro-driven virtual screening on a desktop computer grid

Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors.

Polymerases of hepatitis C viruses and flaviviruses: structural and mechanistic insights and drug development

Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling

Purification and crystallization of dengue and West Nile virus NS2B–NS3 complexes

Small Molecule Inhibitors That Selectively Block Dengue Virus Methyltransferase

Stabilization of dengue virus polymerase in de novo initiation assay provides advantages for compound screening

Strategies for development of Dengue virus inhibitors

Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus.

Structure-activity relationship of uridine-based nucleoside phosphoramidate prodrugs for inhibition of dengue virus RNA-dependent RNA polymerase

Ten years of dengue drug discovery: progress and prospects

The Dengue Virus Replication Complex: From RNA Replication to Protein-Protein Interactions to Evasion of Innate Immunity.

The dengue virus NS5 protein as a target for drug discovery

The human immunodeficiency virus type 1 Tat protein up-regulates the promoter activity of the beta-chemokine monocyte chemoattractant protein 1 in the human astrocytoma cell line U-87 MG: role of SP-1, AP-1, and NF-kappaB consensus sites

Towards the design of antiviral inhibitors against flaviviruses: the case for the multifunctional NS3 protein from Dengue virus as a target

Two RNA tunnel inhibitors bind in highly conserved sites in Dengue virus NS5 polymerase: structural and functional studies

West Nile virus drug discovery

Yellow fever virus NS3 protease: peptide-inhibition studies.