List of works - Diego Orzaez

A Synthetic Biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins.

scientific article published on 29 August 2017

A VIN1 GUS::GFP fusion reveals activated sucrose metabolism programming occurring in interspersed cells during tomato fruit ripening.

scientific article published on 15 April 2013

A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.

scientific article

A functional polymeric immunoglobulin receptor in chicken (Gallus gallus) indicates ancient role of secretory IgA in mucosal immunity.

scientific article published in June 2004

A memory switch for plant synthetic biology based on the phage ϕC31 integration system

scientific article published on 01 April 2020

A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

scientific article published on February 2016

A visual reporter system for virus-induced gene silencing in tomato fruit based on anthocyanin accumulation.

scientific article published on 8 May 2009

Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.

scientific article published in January 2006

Anthocyanins double the shelf life of tomatoes by delaying overripening and reducing susceptibility to gray mold

scientific article

Assessment of Cas12a‐mediated gene editing efficiency in plants

scientific article published on 19 April 2019

Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

scientific article

Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.

scientific article

Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants.

scientific article published on 18 March 2013

DNA assembly standards: Setting the low-level programming code for plant biotechnology

scientific article published on 03 March 2018

Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy.

scientific article published in January 2014

Display and selection of chicken IgA Fab fragments.

scientific article

Dually biofortified cisgenic tomatoes with increased flavonoids and branched‐chain amino acids content

scientific article published in 2023

Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants

scientific article published on 18 December 2015

Elucidation of the first step in betalain biosynthesis allows the heterologous production of betalain pigments in plants

scientific article published on 14 December 2016

Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against rotavirus.

scientific article published on August 2014

Evolutionarily distinct carotenoid cleavage dioxygenases are responsible for crocetin production in Buddleja davidii.

scientific article

Exploiting Multisite Gateway and pENFRUIT plasmid collection for fruit genetic engineering

scientific article published on 01 January 2012

FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology

scientific article published on 20 April 2018

GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data.

scientific article

GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.

scientific article published in July 2013

GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules

scientific article (publication date: 2011)

Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword?

scientific article (publication date: 10 April 2014)

Multigene Engineering by GoldenBraid Cloning: From Plants to Filamentous Fungi and Beyond

scientific article published on 01 March 2020

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.

scientific article published on 28 June 2017

Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

scientific article published on 10 November 2011

New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

scientific article

Programme of senescence in petals and carpels of Pisum sativum L. flowers and its control by ethylene.

scientific article published in April 1999

Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity

scientific article published on 07 December 2012

Reverse genetics and transient gene expression in fleshy fruits: overcoming plant stable transformation.

scientific article published on 29 September 2009

Software-assisted stacking of gene modules using GoldenBraid 2.0 DNA-assembly framework.

scientific article published in January 2015

Standards for plant synthetic biology: a common syntax for exchange of DNA parts.

scientific article

Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator

scientific article published on 23 May 2019

The plant homologue of the defender against apoptotic death gene is down-regulated during senescence of flower petals.

scientific article published in March 1997

Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants

scientific article published on 29 January 2016

Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population.

scientific article

VIGS: a tool to study fruit development in Solanum lycopersicum.

scientific article published in January 2013

List of works by Diego Orzaez

A Synthetic Biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins.

A VIN1 GUS::GFP fusion reveals activated sucrose metabolism programming occurring in interspersed cells during tomato fruit ripening.

A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein.

A functional polymeric immunoglobulin receptor in chicken (Gallus gallus) indicates ancient role of secretory IgA in mucosal immunity.

A memory switch for plant synthetic biology based on the phage ϕC31 integration system

A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

A visual reporter system for virus-induced gene silencing in tomato fruit based on anthocyanin accumulation.

Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.

Anthocyanins double the shelf life of tomatoes by delaying overripening and reducing susceptibility to gray mold

Assessment of Cas12a‐mediated gene editing efficiency in plants

Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.

Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants.

DNA assembly standards: Setting the low-level programming code for plant biotechnology

Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy.

Display and selection of chicken IgA Fab fragments.

Dually biofortified cisgenic tomatoes with increased flavonoids and branched‐chain amino acids content

Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants

Elucidation of the first step in betalain biosynthesis allows the heterologous production of betalain pigments in plants

Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against rotavirus.

Evolutionarily distinct carotenoid cleavage dioxygenases are responsible for crocetin production in Buddleja davidii.

Exploiting Multisite Gateway and pENFRUIT plasmid collection for fruit genetic engineering

FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology

GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data.

GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.

GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules

Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword?

Multigene Engineering by GoldenBraid Cloning: From Plants to Filamentous Fungi and Beyond

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.

Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

Programme of senescence in petals and carpels of Pisum sativum L. flowers and its control by ethylene.

Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity

Reverse genetics and transient gene expression in fleshy fruits: overcoming plant stable transformation.

Software-assisted stacking of gene modules using GoldenBraid 2.0 DNA-assembly framework.

Standards for plant synthetic biology: a common syntax for exchange of DNA parts.

Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator

The plant homologue of the defender against apoptotic death gene is down-regulated during senescence of flower petals.

Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants

Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population.

VIGS: a tool to study fruit development in Solanum lycopersicum.