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List of works by Joanna Kowalska

2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion

2’- O -methylation of the second transcribed nucleotide within mRNA 5’ cap impacts protein production level in a cell specific manner and contributes to RNA immune evasion

scientific article published on 3 February 2022

5'-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes.

scientific article published on 20 April 2018

5'-fluoro(di)phosphate-labeled oligonucleotides are versatile molecular probes for studying nucleic acid secondary structure and interactions by 19F NMR

scientific article published on 09 June 2020

7-Methylguanosine monophosphate analogues with 5'-(1,2,3-triazoyl) moiety: Synthesis and evaluation as the inhibitors of cNIIIB nucleotidase.

scientific article published on 22 November 2017

A fluorescent HTS assay for phosphohydrolases based on nucleoside 5'-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I.

scientific article

A novel route for preparing 5' cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry

scientific article

A simple and rapid synthesis of nucleotide analogues containing a phosphorothioate moiety at the terminal position of the phosphate chain

scientific article

Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to decapping scavenger activity monitoring

scientific article published on 15 March 2016

Adenosine 5'-tetraphosphate is a highly potent purinergic endothelium-derived vasoconstrictor.

scientific article published on 2 October 2008

Amino-Functionalized 5' Cap Analogs as Tools for Site-Specific Sequence-Independent Labeling of mRNA.

scientific article

Analysis of decapping scavenger cap complex using modified cap analogs reveals molecular determinants for efficient cap binding.

scientific article published on 23 October 2013

Analysis of mononucleotides by tandem mass spectrometry: investigation of fragmentation pathways for phosphate- and ribose-modified nucleotide analogues

scientific article

Applications of Phosphate Modification and Labeling to Study (m)RNA Caps

scientific article

Assignment of the absolute configuration of P-chiral 5' mRNA cap analogues containing phosphorothioate moiety

scientific article published in January 2007

Azido-Functionalized 5' Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells

scientific article

Azido-Functionalized 5′ Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells

scientific article

Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation

scientific article

Biomolecular condensates amplify mRNA decapping by coupling protein interactions with conformational changes in Dcp1/Dcp2

scientific article

Cap analogs containing 6-thioguanosine--reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules.

scientific article published in July 2014

Cap analogs modified with 1,2-dithiodiphosphate moiety protect mRNA from decapping and enhance its translational potential

scientific article published on 07 October 2016

Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties

Clickable trimethylguanosine cap analogs modified within the triphosphate bridge: synthesis, conjugation to RNA and susceptibility to degradation

scientific article

Cover Feature: Fluorescent Turn‐On Probes for the Development of Binding and Hydrolytic Activity Assays for mRNA Cap‐Recognizing Proteins (Chem. Eur. J. 27/2019)

scientific article

DXO/Rai1 enzymes remove 5'-end FAD and dephospho-CoA caps on RNAs

scientific article published on 06 May 2020

Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity

scientific article published on 07 April 2020

Drosophila miR2 primarily targets the m7GpppN cap structure for translational repression

scientific article

Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP).

scientific article published in January 2010

Efficient and Rapid Synthesis of Nucleoside Diphosphate Sugars from Nucleoside Phosphorimidazolides

Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation

scientific article

Ethynyl, 2-Propynyl, and 3-Butynyl C-Phosphonate Analogues of Nucleoside Di- and Triphosphates: Synthesis and Reactivity in CuAAC.

scientific article published on 29 May 2015

Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy

scientific article

ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions

scientific article published on 01 August 2018

Exploring the potential of phosphotriazole 5' mRNA cap analogues as efficient translation initiators

scientific article published on 01 September 2018

Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding

scientific article published on 18 November 2014

Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9

scientific article published on 16 May 2022

Fluorescent Turn-On Probes for the Development of Binding and Hydrolytic Activity Assays for mRNA Cap-Recognizing Proteins

scientific article published on 01 April 2019

Fluorinated Phosphoadenosine 5′-Phosphosulfate Analogues for Continuous Sulfotransferase Activity Monitoring and Inhibitor Screening by 19 F NMR Spectroscopy

Functional and LC-MS/MS analysis ofin vitrotranscribed mRNAs carrying phosphorothioate or boranophosphate moieties reveal polyA tail modifications that prevent deadenylation without compromising protein expression

scientific article

Gold-decorated polymer vessel structures as carriers of mRNA cap analogs

Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase

scientific article

Interaction of human decapping scavenger with 5′ mRNA cap analogues: structural requirements for catalytic activity

Introducing SuFNucs: Sulfamoyl-Fluoride-Functionalized Nucleosides That Undergo Sulfur Fluoride Exchange Reaction

scientific article published in 2022

Kinetic analysis of IFIT1 and IFIT5 interactions with different native and engineered RNAs and its consequences for designing mRNA-based therapeutics

scientific article published on 28 October 2019

Magnetic-nanoparticle-decorated polypyrrole microvessels: toward encapsulation of mRNA cap analogues.

scientific article

Methylene analogues of adenosine 5'-tetraphosphate. Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases.

scientific article published in February 2006

N1-Propargylguanosine Modified mRNA Cap Analogs: Synthesis, Reactivity, and Applications to the Study of Cap-Binding Proteins.

scientific article published on 17 May 2019

NAD Analogs in Aid of Chemical Biology and Medicinal Chemistry

scientific article published on 19 November 2019

Nicotinamide-Containing Di- and Trinucleotides as Chemical Tools for Studies of NAD-Capped RNAs

scientific article published on 27 November 2018

Novel N7-Arylmethyl Substituted Dinucleotide mRNA 5' cap Analogs: Synthesis and Evaluation as Modulators of Translation

scholarly article by Radoslaw Wojcik et al published 16 November 2021 in Pharmaceutics

Phosphate-modified analogues of m(7)GTP and m(7)Gppppm(7)G-Synthesis and biochemical properties

scientific article published on 29 July 2015

Phosphodiester modifications in mRNA polyA tail prevent deadenylation without compromising protein expression

scientific article published on 20 August 2020

Phosphoroselenoate dinucleotides for modification of mRNA 5' end

scientific article published on 01 October 2009

Phosphorothioate analogs of m7GTP are enzymatically stable inhibitors of cap-dependent translation

scientific article published on 21 February 2009

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

scientific article published on 22 April 2010

Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells

scientific article

Potential therapeutic applications of RNA cap analogs.

scientific article published on June 2013

Preparation of synthetically challenging nucleotides using cyanoethyl P-imidazolides and microwaves

scientific article published on 11 September 2012

Quick Access to Nucleobase-Modified Phosphoramidites for the Synthesis of Oligoribonucleotides Containing Post-Transcriptional Modifications and Epitranscriptomic Marks

RNA Ligation for Mono and Dually Labeled RNAs

scientific article

Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation

scientific article published in Nature Communications

Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect

scientific article

Substrate-Based Design of Cytosolic Nucleotidase IIIB Inhibitors and Structural Insights into Inhibition Mechanism

scientific article published on 29 April 2022

Synthesis and characterization of mRNA cap analogs containing phosphorothioate substitutions that bind tightly to eIF4E and are resistant to the decapping pyrophosphatase DcpS

scientific article published on 22 April 2008

Synthesis and enzymatic characterization of methylene analogs of adenosine 5'-tetraphosphate (P4A).

scientific article

Synthesis and properties of mRNA cap analogs containing imidodiphosphate moiety—fairly mimicking natural cap structure, yet resistant to enzymatic hydrolysis

scientific article published on 20 January 2012

Synthesis and properties of mRNA cap analogs containing phosphorothioate moiety in 5',5'-triphosphate chain

scientific article published on 01 January 2005

Synthesis of RNA 5'-Azides from 2'-O-Pivaloyloxymethyl-Protected RNAs and Their Reactivity in Azide-Alkyne Cycloaddition Reactions

scientific article published on 21 June 2017

Synthesis of Trifluoromethylated Purine Ribonucleotides and Their Evaluation as 19F NMR Probes

scientific article published on 13 February 2020

Synthesis of biotin labelled cap analogue--incorporable into mRNA transcripts and promoting cap-dependent translation

scientific article published on 01 November 2012

Synthesis of nucleoside phosphosulfates

scientific article published on 20 April 2012

Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes

scientific article

Synthetic dinucleotide mRNA cap analogs with tetraphosphate 5',5' bridge containing methylenebis(phosphonate) modification

scientific article published on 7 September 2009

Synthetic mRNA cap analogs with a modified triphosphate bridge – synthesis, applications and prospects

Synthetic mRNAs with superior translation and stability properties.

scientific article published on January 2013

The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5' cap modulates protein expression in living cells

scientific article published on 01 February 2020

The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5’ cap modulates protein expression in living cells

scientific article

The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation.

scientific article

Towards mRNA with superior translational activity: synthesis and properties of ARCA tetraphosphates with single phosphorothioate modifications

scientific article

Towards novel efficient and stable nuclear import signals: synthesis and properties of trimethylguanosine cap analogs modified within the 5′,5′-triphosphate bridge

scientific article published on 01 December 2014

Translation initiation factor 4E in complex with m2(7,2'O)GppCCl2ppG mRNA 5' cap analog

Translation initiation factor 4E in complex with m7GppppG mRNA 5' cap analog

Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH3, Se, and NH

scientific article

Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex.

scientific article published on 29 January 2016

Virus-like particle-mediated intracellular delivery of mRNA cap analog with in vivo activity against hepatocellular carcinoma

scientific article

mRNA Cap Modification through Carbamate Chemistry: Synthesis of Amino- and Carboxy-Functionalised Cap Analogues Suitable for Labelling and Bioconjugation

scientific article

mRNAs biotinylated within the 5' cap and protected against decapping: new tools to capture RNA-protein complexes

scientific article published on 05 November 2018

mRNAs containing the histone 3' stem-loop are degraded primarily by decapping mediated by oligouridylation of the 3' end.

scientific article

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