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List of works by Tetsushi Sakuma

A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector

scientific article published on 17 March 2015

ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair

scientific article published on 17 November 2017

ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk

scientific article published on 22 July 2020

Biased genome editing using the local accumulation of DSB repair molecules system

scientific article published in Nature Communications

C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection

scientific article published on 16 November 2016

Cas9, Cpf1 and C2c1/2/3-What's next?

scientific article published on 31 January 2017

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.

scientific article published on 29 April 2015

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice.

scientific article

Depdc5 knockout rat: A novel model of mTORopathy

scientific article published on 9 February 2016

Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins

scientific article published on 13 May 2015

Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant.

scientific article published on 22 October 2016

Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies

scientific article published on 27 August 2014

EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step

scientific article

EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD

scientific article published on 17 February 2020

Efficient TALEN construction and evaluation methods for human cell and animal applications.

scientific article published on 6 February 2013

Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases ⬇️

scientific article published on January 3, 2013

Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES-2145

scientific article published on 05 September 2020

Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes ⬇️

scientific article published on February 13, 2013

Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets.

scientific article published on 21 October 2015

Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases

scientific article published on 28 May 2012

Enhancer activity sensitive to the orientation of the gene it regulates in the chordategenome ⬇️

scientific article published on December 27, 2012

Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9.

scientific article

Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells.

scientific article published on 16 March 2016

Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO.

scientific article

Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease

scientific article published on 20 October 2017

FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions

scientific article

Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation

scientific article (publication date: 2016)

Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa

scientific article published on 20 June 2016

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

scientific article

Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9

scientific article published on 9 June 2015

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9.

scientific article

Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.

scientific article published on 19 March 2014

Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells.

scientific article published in February 2017

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

scientific article published on 4 March 2013

Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system.

scientific article

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

scientific article

Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus

scientific article published on 23 September 2016

Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs

scientific article published on 29 April 2015

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids.

scientific article

Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx.

scientific article published on 13 March 2017

Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine

scientific article

Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation

scientific article published on 19 November 2015

In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration

scientific article published on 24 February 2016

MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner

article

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

scientific article published on 17 December 2015

Magic wands of CRISPR-lots of choices for gene knock-in.

scientific article

Microhomology-assisted scarless genome editing in human iPSCs.

scientific article published on 5 March 2018

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

scientific article

Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology

scientific article published on 9 November 2017

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

scientific article published on 23 June 2014

Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy

scientific article published on 24 November 2016

Nuclease-mediated genome editing: At the front-line of functional genomics technology

scientific article published on 05 January 2014

PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells.

scientific article published on 3 July 2017

PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.

scientific article

Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.

scientific article published on 26 November 2014

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

scientific article

Production of Sry knockout mouse using TALEN via oocyte injection.

scientific article

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

scientific article published on 22 May 2015

Quantitative assay for TALEN activity at endogenous genomic loci

scientific article

Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines

scientific article

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.

scientific article

Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells

scientific article published on 16 July 2015

Simple knockout by electroporation of engineered endonucleases into intact rat embryos

scientific article

Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency.

scientific article published on May 2016

Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.

scientific article published on 18 June 2015

Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato

scientific article published on 12 September 2014

Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells

scientific article

Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities.

scientific article published on 27 April 2016

TALEN-Mediated Gene Editing of slc24a5 (Solute Carrier Family 24, Member 5) in Kawakawa, Euthynnus affinis

scientific article published in 2021

TALEN-induced gene knock out in Drosophila

scientific article

TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome.

scientific article published on 16 December 2013

Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus

article

Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex

scientific article

Targeted mutagenesis in sea urchin embryos using TALENs.

scientific article published on 21 November 2013

Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases.

scientific article published on 11 December 2013

Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish

scientific article published on 29 March 2016

The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin

scientific article published on 25 January 2013

The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos

scientific article published on 18 November 2015

The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation.

scientific article published on 04 February 2015

The third type III module of human fibronectin mediates cell adhesion and migration.

scientific article published on 29 October 2009

Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona.

scientific article published on 18 December 2013

Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.

scientific article published on 11 December 2013

Transcriptional regulation of a horizontally transferred gene from bacterium to chordate.

scientific article published in December 2016

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.

scientific article published on 07 July 2016

Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus

scientific article published on 9 February 2018

Unliganded thyroid hormone receptor α regulates developmental timing via gene repression in Xenopus tropicalis

scientific article

Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies

scientific article published on 08 June 2020

Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans

scientific article published on 17 December 2013

Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos.

scientific article

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