List of works - Daniel Ladant

3'-5' phosphoadenosine phosphate is an inhibitor of PARP-1 and a potential mediator of the lithium-dependent inhibition of PARP-1 in vivo

scientific article

A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning

scientific article published on 01 January 2018

A Gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system.

scientific article

A bacterial two-hybrid system based on a reconstituted signal transduction pathway

scientific article (publication date: 12 May 1998)

A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli

scientific article published on 01 January 2000

Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins.

scientific article

Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli.

scientific article

Affinity-based chromatography utilizing genetically engineered proteins. Interaction of Bordetella pertussis adenylate cyclase with calmodulin

scientific article published on 01 March 1988

Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies

scientific article published on July 2014

An increase in antimycobacterial Th1-cell responses by prime-boost protocols of immunization does not enhance protection against tuberculosis

scientific article

Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique.

scientific article

Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY

scientific article

Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay

scientific article

Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases.

scientific article

Antigen targeting to CD11b+ dendritic cells in association with TLR4/TRIF signaling promotes strong CD8+ T cell responses

scientific article published on 14 July 2014

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

scientific article published on 20 July 2018

Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo.

scientific article published in December 2004

Bordetella pertussis adenylate cyclase toxin as a tool to analyze molecular interactions in a bacterial two-hybrid system

scientific article published on 01 October 2000

Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer

scientific article published on 02 December 2013

Bordetella pertussis adenylate cyclase toxin: a vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells

scientific article published on 01 January 2000

Bordetella pertussis adenylate cyclase. Purification, characterization, and radioimmunoassay.

scientific article

Bordetella pertussis adenylate cyclase: a toxin with multiple talents

scientific article published on 01 April 1993

Calcium and membrane binding properties of bovine neurocalcin delta expressed in Escherichia coli

scientific article

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

scientific article

Calcium-dependent disorder-to-order transitions are central to the secretion and folding of the CyaA toxin of Bordetella pertussis, the causative agent of whooping cough.

scientific article

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

scientific article

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

scientific article published on 29 December 2017

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

scientific article published in January 2010

Cell entry and cAMP imaging of anthrax edema toxin

scientific article published on 2 November 2006

Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery

scientific article

Characterization of a beta-galactosidase hybrid protein carrying the catalytic domain of Escherichia coli adenylate cyclase

scientific article published on 01 September 1986

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

scientific article published on 24 September 2013

Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

scientific article

Characterization of mutant Bordetella pertussis adenylate cyclase toxins with reduced affinity for calmodulin. Implications for the mechanism of toxin entry into target cells

scientific article published on 01 February 1997

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin.

scientific article published on 22 January 2010

Charge-dependent translocation of Bordetella pertussis adenylate cyclase toxin into eukaryotic cells: implication for the in vivo delivery of CD8(+) T cell epitopes into antigen-presenting cells.

scientific article published on October 1998

Compartmentalized Cyclic AMP Production by the Bordetella pertussis and Bacillus anthracis Adenylate Cyclase Toxins Differentially Affects the Immune Synapse in T Lymphocytes.

scientific article

Correction: Anthrax Edema Toxin Modulates PKA- and CREB-Dependent Signaling in Two Phases.

scientific article published on 5 November 2008

Defining Membrane Protein Topology Using pho-lac Reporter Fusions

scientific article published in January 2017

Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates.

scientific article published on 21 February 2006

Differential mechanisms for calcium-dependent protein/membrane association as evidenced from SPR-binding studies on supported biomimetic membranes.

scientific article published in December 2003

Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin

scientific article published in PLoS ONE

Direct delivery of the Bordetella pertussis adenylate cyclase toxin to the MHC class I antigen presentation pathway.

scientific article published on February 1999

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

scientific article

Editorial: Pathogens and disease special issue ETOX18

scientific article published on 01 March 2019

Efficient display of two enzymes on filamentous phage using an improved signal sequence.

scientific article

Eradication of established tumors by vaccination with recombinant Bordetella pertussis adenylate cyclase carrying the human papillomavirus 16 E7 oncoprotein.

scientific article published in January 2005

Eradication of large tumors in mice by a tritherapy targeting the innate, adaptive, and regulatory components of the immune system

scientific article published on 01 September 2007

Essential role of methionine residues in calmodulin binding to Bordetella pertussis adenylate cyclase, as probed by selective oxidation and repair by the peptide methionine sulfoxide reductases.

scientific article

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

scientific article published on 01 January 2012

ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation.

scientific article

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview.

scientific article published on 16 December 2017

Exposure to Bordetella pertussis adenylate cyclase toxin affects integrin-mediated adhesion and mechanics in alveolar epithelial cells.

scientific article

Expression and characterization of calcium-myristoyl switch proteins.

scientific article published on January 1995

Human immunodeficiency virus (HIV) type 1 transframe protein can restore activity to a dimerization-deficient HIV protease variant.

scientific article

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin.

scientific article published on 12 January 2012

In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying viral CD8+ T cell epitopes

scientific article published on 01 June 1996

In vivo receptor-mediated delivery of a recombinant invasive bacterial toxoid to CD11c + CD8 alpha -CD11bhigh dendritic cells

scientific article published on November 2002

Insertional mutagenesis of Bordetella pertussis adenylate cyclase ⬇️

scientific article published on February 5, 1992

Interaction Network among Membrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis

scientific article published in 2008

Interaction of Bordetella pertussis adenylate cyclase with CD11b/CD18: Role of toxin acylation and identification of the main integrin interaction domain

scientific article published on 28 July 2003

Interaction of Bordetella pertussis adenylate cyclase with calmodulin. Identification of two separated calmodulin-binding domains

scientific article published on 01 February 1988

Interaction of Calcium withBordetella pertussisAdenylate Cyclase Toxin

scientific article published in Journal of Biological Chemistry

Lack of MHC class II molecules favors CD8+ T-cell infiltration into tumors associated with an increased control of tumor growth.

scientific article published on 7 December 2017

Large-scale study of the interactions between proteins involved in type IV pilus biology in Neisseria meningitidis: characterization of a subcomplex involved in pilus assembly

scientific article published on 24 April 2012

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements.

scientific article published in January 2012

Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region

scientific article published on 14 November 2017

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein.

scientific article published on 31 July 2013

Oral macrophage-like cells play a key role in tolerance induction following sublingual immunotherapy of asthmatic mice

scientific article published on 20 July 2011

Post-translational acylation controls the folding and functions of the CyaA RTX toxin

scientific article published on 21 June 2019

Protein-Protein Interaction: Bacterial Two-Hybrid.

scientific article

Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system.

scientific article

Purification and immunological characterization of bacterial and secreted adenylate cyclase of "Bordetella pertussis"

scientific article published on 01 January 1986

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion.

scientific article published on 17 November 2008

Rapamycin Impairs Antitumor CD8+ T-cell Responses and Vaccine-Induced Tumor Eradication

scientific article

Recombinant adenylate cyclase toxin of Bordetella pertussis induces cytotoxic T lymphocyte responses against HLA*0201-restricted melanoma epitopes.

scientific article

Repeat sequences in the Bordetella pertussis adenylate cyclase toxin can be recognized as alternative carboxy-proximal secretion signals by the Escherichia coli alpha-haemolysin translocator.

scientific article

Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB.

scientific article

SEC-SAXS and HDX-MS: A powerful combination. The case of the calcium-binding domain of a bacterial toxin.

scientific article published on 2 August 2017

Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli.

scientific article

Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis.

scientific article published on 10 February 2017

Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin.

scientific article

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion

scientific article (publication date: 2015)

Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review).

scientific article

The Adenylate Cyclase (CyaA) Toxin from Has No Detectable Phospholipase A (PLA) Activity In Vitro

scientific article published on 13 February 2019

The adenylate cyclase of Bordetella pertussis: a vector to target antigen presenting cells.

scientific article

The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18)

scientific article

The adenylate cyclase toxins of Bacillus anthracis and Bordetella pertussis promote Th2 cell development by shaping T cell antigen receptor signaling

scientific article

The effector protein ExoY secreted by Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for GTP.

scientific article published in August 2011

The extreme C-terminus of the effector ExoY is crucial for binding to its eukaryotic activator, F-actin

scientific article published in Journal of Biological Chemistry

The β-lactam resistance protein Blr, a small membrane polypeptide, is a component of the Escherichia coli cell division machinery.

scientific article

Therapy of murine tumors with recombinant Bordetella pertussis adenylate cyclase carrying a cytotoxic T cell epitope

scientific article published on 01 April 1999

Translocation and calmodulin-activation of the adenylate cyclase toxin (CyaA) of Bordetella pertussis

scientific article published on 01 November 2018

Two-hybrid systems and their usage in infection biology.

scientific article

Use of allostery to identify inhibitors of calmodulin-induced activation of Bacillus anthracis edema factor.

scientific article

ZapE is a novel cell division protein interacting with FtsZ and modulating the Z-ring dynamics

scientific article published on 04 March 2014

cAMP assays

scientific article published on 01 April 2007

List of works by Daniel Ladant

3'-5' phosphoadenosine phosphate is an inhibitor of PARP-1 and a potential mediator of the lithium-dependent inhibition of PARP-1 in vivo

A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning

A Gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system.

A bacterial two-hybrid system based on a reconstituted signal transduction pathway

A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli

Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins.

Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli.

Affinity-based chromatography utilizing genetically engineered proteins. Interaction of Bordetella pertussis adenylate cyclase with calmodulin

Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies

An increase in antimycobacterial Th1-cell responses by prime-boost protocols of immunization does not enhance protection against tuberculosis

Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique.

Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY

Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay

Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases.

Antigen targeting to CD11b+ dendritic cells in association with TLR4/TRIF signaling promotes strong CD8+ T cell responses

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

Bordetella pertussis adenylate cyclase delivers chemically coupled CD8+ T-cell epitopes to dendritic cells and elicits CTL in vivo.

Bordetella pertussis adenylate cyclase toxin as a tool to analyze molecular interactions in a bacterial two-hybrid system

Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer

Bordetella pertussis adenylate cyclase toxin: a vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells

Bordetella pertussis adenylate cyclase. Purification, characterization, and radioimmunoassay.

Bordetella pertussis adenylate cyclase: a toxin with multiple talents

Calcium and membrane binding properties of bovine neurocalcin delta expressed in Escherichia coli

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

Calcium-dependent disorder-to-order transitions are central to the secretion and folding of the CyaA toxin of Bordetella pertussis, the causative agent of whooping cough.

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

Cell entry and cAMP imaging of anthrax edema toxin

Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery

Characterization of a beta-galactosidase hybrid protein carrying the catalytic domain of Escherichia coli adenylate cyclase

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis

Characterization of mutant Bordetella pertussis adenylate cyclase toxins with reduced affinity for calmodulin. Implications for the mechanism of toxin entry into target cells

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin.

Charge-dependent translocation of Bordetella pertussis adenylate cyclase toxin into eukaryotic cells: implication for the in vivo delivery of CD8(+) T cell epitopes into antigen-presenting cells.

Compartmentalized Cyclic AMP Production by the Bordetella pertussis and Bacillus anthracis Adenylate Cyclase Toxins Differentially Affects the Immune Synapse in T Lymphocytes.

Correction: Anthrax Edema Toxin Modulates PKA- and CREB-Dependent Signaling in Two Phases.

Defining Membrane Protein Topology Using pho-lac Reporter Fusions

Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates.

Differential mechanisms for calcium-dependent protein/membrane association as evidenced from SPR-binding studies on supported biomimetic membranes.

Differential regulation of actin-activated nucleotidyl cyclase virulence factors by filamentous and globular actin

Direct delivery of the Bordetella pertussis adenylate cyclase toxin to the MHC class I antigen presentation pathway.

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

Editorial: Pathogens and disease special issue ETOX18

Efficient display of two enzymes on filamentous phage using an improved signal sequence.

Eradication of established tumors by vaccination with recombinant Bordetella pertussis adenylate cyclase carrying the human papillomavirus 16 E7 oncoprotein.

Eradication of large tumors in mice by a tritherapy targeting the innate, adaptive, and regulatory components of the immune system

Essential role of methionine residues in calmodulin binding to Bordetella pertussis adenylate cyclase, as probed by selective oxidation and repair by the peptide methionine sulfoxide reductases.

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

ExoY from Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for cGMP and cUMP formation.

ExoY, an actin-activated nucleotidyl cyclase toxin from P. aeruginosa: A minireview.

Exposure to Bordetella pertussis adenylate cyclase toxin affects integrin-mediated adhesion and mechanics in alveolar epithelial cells.

Expression and characterization of calcium-myristoyl switch proteins.

Human immunodeficiency virus (HIV) type 1 transframe protein can restore activity to a dimerization-deficient HIV protease variant.

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin.

In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying viral CD8+ T cell epitopes

In vivo receptor-mediated delivery of a recombinant invasive bacterial toxoid to CD11c + CD8 alpha -CD11bhigh dendritic cells

Insertional mutagenesis of Bordetella pertussis adenylate cyclase ⬇️

Interaction Network among Membrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis

Interaction of Bordetella pertussis adenylate cyclase with CD11b/CD18: Role of toxin acylation and identification of the main integrin interaction domain

Interaction of Bordetella pertussis adenylate cyclase with calmodulin. Identification of two separated calmodulin-binding domains

Interaction of Calcium withBordetella pertussisAdenylate Cyclase Toxin

Lack of MHC class II molecules favors CD8+ T-cell infiltration into tumors associated with an increased control of tumor growth.

Large-scale study of the interactions between proteins involved in type IV pilus biology in Neisseria meningitidis: characterization of a subcomplex involved in pilus assembly

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements.

Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein.

Oral macrophage-like cells play a key role in tolerance induction following sublingual immunotherapy of asthmatic mice

Post-translational acylation controls the folding and functions of the CyaA RTX toxin

Protein-Protein Interaction: Bacterial Two-Hybrid.

Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system.

Purification and immunological characterization of bacterial and secreted adenylate cyclase of "Bordetella pertussis"

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion.

Rapamycin Impairs Antitumor CD8+ T-cell Responses and Vaccine-Induced Tumor Eradication

Recombinant adenylate cyclase toxin of Bordetella pertussis induces cytotoxic T lymphocyte responses against HLA*0201-restricted melanoma epitopes.

Repeat sequences in the Bordetella pertussis adenylate cyclase toxin can be recognized as alternative carboxy-proximal secretion signals by the Escherichia coli alpha-haemolysin translocator.

Role of leucine zipper motifs in association of the Escherichia coli cell division proteins FtsL and FtsB.

SEC-SAXS and HDX-MS: A powerful combination. The case of the calcium-binding domain of a bacterial toxin.